Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,797,391 A→C 6.5% L185R (CTG→CGG)  pheT ← phenylalanine tRNA synthetase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,797,3910AC 6.5% 307.0 / 8.7 90L185R (CTG→CGG) pheTphenylalanine tRNA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (41/43);  new base C (3/3);  total (44/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAG  >  NC_000913/1797245‑1797532
                                                                                                                                                  |                                                                                                                                             
tCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGc                                                                                                                                             >  3:730427/1‑149 (MQ=255)
  cacaGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCt                                                                                                                                            <  2:243470/148‑1 (MQ=255)
  cacaGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCt                                                                                                                                            <  1:134818/148‑1 (MQ=255)
   acaGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTg                                                                                                                                           >  2:617382/1‑148 (MQ=255)
     aGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGtt                                                                                                                                        >  4:737033/1‑149 (MQ=255)
      gCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTc                                                                                                                                       <  3:496693/149‑1 (MQ=255)
       cGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCGGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGTTCa                                                                                                                                      <  3:746293/149‑1 (MQ=255)
          aGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGCTGAACCAGCTGCCGCTGGTTCaac                                                                                                                                    <  2:521971/148‑3 (MQ=255)
              ggCGCTTTAACGTTAATGCCTTTTACCAGACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGc                                                                                                                               >  3:65079/1‑149 (MQ=255)
                   tttAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACg                                                                                                                           >  1:112637/1‑148 (MQ=255)
                     tAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCa                                                                                                                        >  4:30054/1‑149 (MQ=255)
                      aaCGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCa                                                                                                                        <  1:499023/148‑1 (MQ=255)
                        cGTTAATGCCTTTTACCACACGGCTAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCAcg                                                                                                                      >  1:242981/1‑148 (MQ=255)
                        cGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCAcg                                                                                                                      <  1:824579/148‑1 (MQ=255)
                          ttAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCAcgcg                                                                                                                    <  1:263129/148‑1 (MQ=255)
                                ccTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACAcc                                                                                                             <  3:410515/149‑1 (MQ=255)
                                ccTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACAcc                                                                                                             >  3:6243/1‑149 (MQ=255)
                                  ttttACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCaa                                                                                                           >  4:70694/1‑149 (MQ=255)
                                  ttttACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCaa                                                                                                           >  3:53478/1‑149 (MQ=255)
                                  ttttACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCa                                                                                                            >  1:58109/1‑148 (MQ=255)
                                  ttttACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCa                                                                                                            >  1:898121/1‑148 (MQ=255)
                                   tttACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCaa                                                                                                           >  2:263621/1‑148 (MQ=255)
                                   tttACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCaa                                                                                                           <  2:827245/148‑1 (MQ=255)
                                   tttACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAat                                                                                                          <  3:25889/149‑1 (MQ=255)
                                   tttACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAat                                                                                                          <  4:520536/149‑1 (MQ=255)
                                      aCCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAatgat                                                                                                       <  4:390146/149‑1 (MQ=255)
                                       ccACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAatgat                                                                                                       >  1:370800/1‑148 (MQ=255)
                                        cacaCGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAatgatg                                                                                                      <  1:229841/148‑1 (MQ=255)
                                        cacaCGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGc                                                                                                     <  3:97844/149‑1 (MQ=255)
                                           acGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACg                                                                                                                           <  2:209764/124‑1 (MQ=255)
                                           acGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACg                                                                                                                           >  1:209764/1‑124 (MQ=255)
                                                cAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAg                                                                                             >  4:369923/1‑149 (MQ=255)
                                                  aGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGt                                                                                            <  1:937169/148‑1 (MQ=255)
                                                             gCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGttt                                                                                 <  2:112637/148‑1 (MQ=255)
                                                              cAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTgg                                                                               <  3:706973/149‑1 (MQ=255)
                                                                  cTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGt                                                                            >  1:130780/1‑148 (MQ=255)
                                                                  cTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTc                                                                           <  4:571103/149‑1 (MQ=255)
                                                                      cGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACg                                                                        <  1:528011/148‑1 (MQ=255)
                                                                          gcTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGAt                                                                   >  3:534292/1‑149 (MQ=255)
                                                                            ttCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAg                                                                                                                                              <  2:588072/72‑1 (MQ=255)
                                                                            ttCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAg                                                                                                                                              >  1:588072/1‑72 (MQ=255)
                                                                             tCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGAttt                                                                 <  1:273315/148‑1 (MQ=255)
                                                                             tCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGAttt                                                                 >  1:295309/1‑148 (MQ=255)
                                                                               gACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCg                                                               <  2:616135/148‑1 (MQ=255)
                                                                               gACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGa                                                              >  3:429170/1‑149 (MQ=255)
                                                                               gACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGa                                                              <  4:156602/149‑1 (MQ=255)
                                                                                aCTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGa                                                              <  2:569317/148‑1 (MQ=255)
                                                                                  tGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATgg                                                           >  3:642791/1‑149 (MQ=255)
                                                                                  tGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATgg                                                           >  3:899437/1‑149 (MQ=255)
                                                                                  tGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATgg                                                           >  4:690777/1‑149 (MQ=255)
                                                                                      aTCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGgtgt                                                        <  2:829840/148‑1 (MQ=255)
                                                                                      aTCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGtgtt                                                       <  4:42028/149‑1 (MQ=255)
                                                                                       tCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGtgttg                                                      >  4:612896/1‑149 (MQ=255)
                                                                                        cGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGtgttg                                                      >  2:24388/1‑148 (MQ=255)
                                                                                          gCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAAAACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGtca                                                   >  4:120147/1‑149 (MQ=255)
                                                                                              cGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGtcatca                                                <  2:242981/148‑1 (MQ=255)
                                                                                              cGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGtcatca                                                <  1:617382/148‑1 (MQ=255)
                                                                                                tgtCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGt                                             <  3:185863/149‑1 (MQ=255)
                                                                                                  tcgtcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGttt                                           >  4:349445/1‑149 (MQ=255)
                                                                                                  tcgtcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGtt                                            <  1:747194/148‑1 (MQ=255)
                                                                                                    gtcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTc                                          <  1:24388/148‑1 (MQ=255)
                                                                                                     tcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAg                                        <  3:346850/149‑1 (MQ=255)
                                                                                                     tcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAg                                        <  4:146649/149‑1 (MQ=255)
                                                                                                        aTGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTa                                     >  4:643102/1‑149 (MQ=255)
                                                                                                         tgttCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGGTTGGCGTCACGATGATTTCGATGGCGTTGTCATCAAGTTTCAGGTAt                                    >  4:282195/4‑149 (MQ=255)
                                                                                                         tGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCa                                         <  2:90831/144‑1 (MQ=255)
                                                                                                         tGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCa                                         >  1:90831/1‑144 (MQ=255)
                                                                                                         tGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTAt                                    >  3:858553/1‑149 (MQ=255)
                                                                                                             cGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCa                                 <  1:462181/148‑1 (MQ=255)
                                                                                                              gCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTc                                          >  4:506629/1‑138 (MQ=255)
                                                                                                              gCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATAAAGTTTc                                          <  3:506629/138‑1 (MQ=255)
                                                                                                                aCCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACgg                              <  2:898121/148‑1 (MQ=255)
                                                                                                                   aaCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGatat                          >  3:371178/1‑149 (MQ=255)
                                                                                                                   aaCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGata                           >  2:543318/1‑148 (MQ=255)
                                                                                                                       ggAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCgg                       <  2:849389/148‑1 (MQ=255)
                                                                                                                       ggAACGATTTCCGGTTGAACCAGCGGCAGCGGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGt                      <  3:538747/149‑1 (MQ=255)
                                                                                                                         aaCGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGc                    <  3:369923/149‑1 (MQ=255)
                                                                                                                          aCGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGc                    >  1:322983/1‑148 (MQ=255)
                                                                                                                           cGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCa                  <  4:371178/149‑1 (MQ=255)
                                                                                                                            gATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCaa                 >  3:95490/1‑149 (MQ=255)
                                                                                                                                 ccGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCTTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCga             <  2:58109/148‑2 (MQ=255)
                                                                                                                                 ccGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCTGTGCCAATCGgc            <  4:182840/149‑1 (MQ=255)
                                                                                                                                    gctGAACCAGCTGCCGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATGTCGGTGCCAATGGGCGCa         >  3:606309/3‑149 (MQ=255)
                                                                                                                                    gTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCa         >  4:645505/1‑149 (MQ=255)
                                                                                                                                       gAACCAGCTGCCGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATc       >  1:813541/1‑148 (MQ=255)
                                                                                                                                         aCCAGCTGCCGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGt                      <  2:944971/131‑1 (MQ=255)
                                                                                                                                         aCCAGCTGCCGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGt                      >  1:944971/1‑131 (MQ=255)
                                                                                                                                         aCCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCgc    >  3:124607/1‑149 (MQ=255)
                                                                                                                                            aGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCgcag  >  2:456036/1‑148 (MQ=255)
                                                                                                                                            aGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCgcag  >  1:724803/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
TCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAG  >  NC_000913/1797245‑1797532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: