Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,880,852 T→A 5.8% intergenic (‑93/+34) leuE ← / ← dmlR leucine efflux protein/transcriptional activator of dmlA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,880,8520TA 5.8% 344.5 / 8.2 101intergenic (‑93/+34)leuE/dmlRleucine efflux protein/transcriptional activator of dmlA
Reads supporting (aligned to +/- strand):  ref base T (50/45);  new base A (4/2);  total (54/47)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.91e-01

Only 100 of 104 total aligned reads displayed.

ATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTGAAACATAAACGGCCCAG  >  NC_000913/1880707‑1881000
                                                                                                                                                 |                                                                                                                                                    
aTGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTaa                                                                                                                                                    <  1:244858/148‑1 (MQ=255)
    ccccAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGa                                                                                                                                               <  4:330928/149‑1 (MQ=255)
    ccccAACCAGATAGGTCCAGTAATTCAGAAACCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTg                                                                                                                                                <  2:89972/148‑1 (MQ=255)
     cccAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGat                                                                                                                                              <  3:319865/149‑1 (MQ=255)
     cccAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGat                                                                                                                                              >  4:411056/1‑149 (MQ=255)
      ccAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAACGGTGAATATGCTAACCAATCTCTCGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGat                                                                                                                                              >  2:609129/1‑148 (MQ=255)
      ccAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGat                                                                                                                                              <  1:10699/148‑1 (MQ=255)
       cAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGata                                                                                                                                             >  2:292604/1‑148 (MQ=255)
        aaCCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATc                                                                                                                                           >  4:748131/1‑149 (MQ=255)
          ccAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCCCGATTAAAGGTGAATATGCAAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCTGGTTTTAACCTGATATCa                                                                                                                                          <  1:450945/148‑1 (MQ=255)
               tAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCg                                                                                                                                     <  1:248369/148‑1 (MQ=255)
                   tcgaGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATaa                                                                                                                                 <  2:50619/145‑1 (MQ=255)
                   tCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATaa                                                                                                                                 >  2:618108/1‑148 (MQ=255)
                    ccAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAAt                                                                                                                                >  2:381637/1‑148 (MQ=255)
                    ccAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAAt                                                                                                                                >  2:141757/1‑148 (MQ=255)
                    ccAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATCAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAAt                                                                                                                                <  2:124686/148‑1 (MQ=255)
                      aGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTg                                                                                                                              >  2:295843/1‑148 (MQ=255)
                       gTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGaa                                                                                                                            <  4:805280/149‑1 (MQ=255)
                       gTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGa                                                                                                                             <  1:430006/148‑1 (MQ=255)
                        tAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAAt                                                                                                                           <  4:505004/149‑1 (MQ=255)
                         aaTTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc                                                                                                                          <  3:224753/149‑1 (MQ=255)
                          aTTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCa                                                                                                                         >  4:819750/1‑149 (MQ=255)
                           ttCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCa                                                                                                                         >  2:167944/1‑148 (MQ=255)
                            tCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCAt                                                                                                                        >  2:215751/1‑148 (MQ=255)
                              aGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTaa                                                                                                                     >  4:811627/1‑149 (MQ=255)
                                  ccccGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCa                                                                                                                  <  1:680988/148‑1 (MQ=255)
                                   cccGTATTCAGCGAACACATTAACCTCTTTACTTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCTGATAATTGAATCATTAAGCAg                                                                                                                 <  2:294749/148‑1 (MQ=255)
                                    ccGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGc                                                                                                               >  3:391101/1‑149 (MQ=255)
                                    ccGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGc                                                                                                               >  3:761800/1‑149 (MQ=255)
                                     cGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGa                                                                                                                             <  1:248692/134‑1 (MQ=255)
                                     cGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGa                                                                                                                             >  2:248692/1‑134 (MQ=255)
                                                aaCACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTg                                                                                                    >  1:515767/1‑148 (MQ=255)
                                                     aTTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAAt                                                                                                                           <  3:676468/120‑1 (MQ=255)
                                                     aTTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAAt                                                                                                                           >  4:676468/1‑120 (MQ=255)
                                                      ttAGCCTCTTTAATTATCTTTGGACCATGCGCGAGTAAAAGTTAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATTAGGTGTTAACCTGATATCAACCCGATAATTGAATCATTCATCAGGCATGCTCCAGTGGAAAAt                                                                                              >  2:76911/1‑148 (MQ=255)
                                                      ttAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAAt                                                                                              <  1:709605/148‑1 (MQ=255)
                                                       tAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAAtt                                                                                             >  2:583970/1‑148 (MQ=255)
                                                        aaCCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCg                                                                                           <  4:53602/149‑1 (MQ=255)
                                                         aCCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGata                                                                                                                                             <  3:868895/98‑1 (MQ=255)
                                                         aCCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCgg                                                                                          <  3:746668/149‑1 (MQ=255)
                                                         aCCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCgg                                                                                          <  3:811627/149‑1 (MQ=255)
                                                         aCCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGACATGCTCCAGTGAAAAATTCg                                                                                           <  1:215751/148‑1 (MQ=255)
                                                           ctctTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGt                                                                                        >  3:137659/1‑149 (MQ=255)
                                                             ctTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTa                                                                                       >  1:226716/1‑148 (MQ=255)
                                                              tttAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAg                                                                                      <  1:365684/148‑1 (MQ=255)
                                                              tttAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGt                                                                                     >  3:348257/1‑149 (MQ=255)
                                                               ttAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGt                                                                                     >  1:352962/1‑148 (MQ=255)
                                                                  aTTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTc                                                                                 <  4:37826/149‑1 (MQ=255)
                                                                   ttATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTcc                                                                                                        <  2:75250/125‑1 (MQ=255)
                                                                   ttATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTcc                                                                                                        >  1:75250/1‑125 (MQ=255)
                                                                   ttATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCg                                                                                <  4:64568/149‑1 (MQ=255)
                                                                   ttATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCg                                                                                >  4:763716/1‑149 (MQ=255)
                                                                        tttCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCaaa                                                                           >  4:385086/1‑149 (MQ=255)
                                                                        tttCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCaaa                                                                           >  4:442098/1‑149 (MQ=255)
                                                                         ttCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCaaaa                                                                          <  3:748131/149‑1 (MQ=255)
                                                                         ttCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCaaa                                                                           >  1:388377/1‑148 (MQ=255)
                                                                         ttCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCaaa                                                                           <  1:76195/148‑1 (MQ=255)
                                                                          tCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCaaaa                                                                          <  1:141757/148‑1 (MQ=255)
                                                                             aTCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATAc                                                                       <  1:807558/148‑1 (MQ=255)
                                                                             aTCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATAc                                                                       >  1:968270/1‑148 (MQ=255)
                                                                             aTCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACt                                                                      <  4:309816/149‑1 (MQ=255)
                                                                              tCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACt                                                                      <  1:252961/148‑1 (MQ=255)
                                                                              tCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACt                                                                      <  1:292604/148‑1 (MQ=255)
                                                                              tCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACt                                                                      >  2:459286/1‑148 (MQ=255)
                                                                               cATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTg                                                                     >  1:735508/1‑148 (MQ=255)
                                                                               cATGCGCGATCAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTg                                                                     >  1:337749/1‑148 (MQ=255)
                                                                                    gcgATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTAAAACCTGat                                                                                                                                              <  4:668565/70‑1 (MQ=255)
                                                                                    gcgATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTAAAACCTGat                                                                                                                                              >  3:668565/1‑70 (MQ=255)
                                                                                           aaGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTc                                                         >  1:613579/1‑148 (MQ=255)
                                                                                           aaGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTc                                                         <  1:423915/148‑1 (MQ=255)
                                                                                            aGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCt                                                        >  2:785686/1‑148 (MQ=255)
                                                                                            aGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCAAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTa                                                       >  3:242512/1‑149 (MQ=255)
                                                                                                    atGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATc                                               <  4:761800/149‑1 (MQ=255)
                                                                                                      gCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCg                                             >  4:43014/1‑149 (MQ=255)
                                                                                                         aaCCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTTAATCATTAATCAGGCATGCACCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTTGCGTAAAAATTCTACCGTTATcagaa                                           >  2:913718/1‑144 (MQ=255)
                                                                                                         aaCCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCAc                                          >  3:483456/1‑149 (MQ=255)
                                                                                                          aCCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCAc                                          >  2:22458/1‑148 (MQ=255)
                                                                                                           ccAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACtt                                        >  4:691111/1‑149 (MQ=255)
                                                                                                            cAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACttt                                       >  3:316241/1‑149 (MQ=255)
                                                                                                                cTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGc                                    <  1:947449/148‑1 (MQ=255)
                                                                                                                      cGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGt                              >  1:252563/1‑148 (MQ=255)
                                                                                                                          ttAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGcc                          >  1:833991/1‑148 (MQ=255)
                                                                                                                             gAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGcc                      <  4:325509/149‑1 (MQ=255)
                                                                                                                               aaGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCt                     >  2:939474/1‑148 (MQ=255)
                                                                                                                               aaGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCt                     >  2:959719/1‑148 (MQ=255)
                                                                                                                                 ggAGAAAATCAGGTTTTAACCTGATATCCACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTGa                  <  4:349604/149‑1 (MQ=255)
                                                                                                                                 ggAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTGa                  >  3:747508/1‑149 (MQ=255)
                                                                                                                                   agaAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTGaaa                <  3:411056/149‑1 (MQ=255)
                                                                                                                                   agaAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTGaaa                <  3:385086/149‑1 (MQ=255)
                                                                                                                                   agaAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTGaa                 >  1:844446/1‑148 (MQ=255)
                                                                                                                                      aaaTCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTGAAACa              >  2:635467/1‑148 (MQ=255)
                                                                                                                                        aTCAGGTTAAAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTg                                                                     >  4:696121/1‑91 (MQ=255)
                                                                                                                                        aTCAGGTTAAAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTg                                                                     <  3:696121/91‑1 (MQ=255)
                                                                                                                                        aTCAGGTTAAAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTGAAACATa            >  1:23922/1‑148 (MQ=255)
                                                                                                                                         tCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTGAAACATaaa          <  3:60576/149‑1 (MQ=255)
                                                                                                                                          cAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTGAAACATAAAc         <  3:866172/149‑1 (MQ=255)
                                                                                                                                            ggTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTGAAACATAAACg        >  2:323458/1‑148 (MQ=255)
                                                                                                                                              ttttAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTGAAACATAAACGGcc     >  3:507899/1‑149 (MQ=255)
                                                                                                                                              ttttAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTGAAACATAAACGGcc     >  3:121738/1‑149 (MQ=255)
                                                                                                                                                 tAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTGAAACATAAACGGCCCAg  >  3:946936/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
ATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTGAAACATAAACGGCCCAG  >  NC_000913/1880707‑1881000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: