Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,985,112 C→G 9.3% intergenic (‑43/+27) araG ← / ← araF L‑arabinose ABC transporter ATPase/L‑arabinose ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,985,1120CG 9.3% 364.8 / 24.8 108intergenic (‑43/+27)araG/araFL‑arabinose ABC transporter ATPase/L‑arabinose ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (53/45);  new base G (7/3);  total (60/48)
Fisher's exact test for biased strand distribution p-value = 5.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.79e-01

Only 100 of 115 total aligned reads displayed.

ACCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTAA  >  NC_000913/1984966‑1985259
                                                                                                                                                  |                                                                                                                                                   
aCCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGcatcat                                                                                                                                                   >  3:933378/1‑149 (MQ=255)
aCCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGcatca                                                                                                                                                    <  2:225744/148‑1 (MQ=255)
 ccTGACAGGCATAGCAGTAAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGcatcat                                                                                                                                                   <  2:733868/148‑1 (MQ=255)
   tGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCGTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGc                                                                                                                                                 <  1:586661/148‑1 (MQ=255)
      ccGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCagag                                                                                                                                             <  4:174856/149‑1 (MQ=255)
       cGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAgg                                                                                                                                            <  3:874560/149‑1 (MQ=255)
        ggCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAggg                                                                                                                                           <  4:317924/149‑1 (MQ=255)
         gCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATGATGCAGAggg                                                                                                                                           <  2:441342/148‑1 (MQ=255)
         gCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGAAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATGATGCAGAggg                                                                                                                                           <  1:509529/148‑1 (MQ=255)
              gCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAAttt                                                                                                                                     <  4:379266/149‑1 (MQ=255)
              gCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGGTTTACCGATGCCGCGAAATGAGAGATACGGGGGAGAATGTTGCATAACGTCTCCGTGAATCACTGGTAGTTCACACCCCCTCTGCATCATGCAGAGGGGAAttt                                                                                                                                     >  4:203784/1‑149 (MQ=255)
               cAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTTGTTCACACCCCCTCTGCATCATGCAGAGGGGAAtttt                                                                                                                                    <  3:228314/149‑1 (MQ=255)
               cAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGTGGGGAAttt                                                                                                                                     >  2:448955/1‑148 (MQ=255)
                  tCAAAACTAATATCCGTCAGCGCTTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTcc                                                                                                                                 <  3:581186/149‑1 (MQ=255)
                  tCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTcc                                                                                                                                 >  3:81091/1‑149 (MQ=255)
                     aaaCTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGc                                                                                                                              >  3:884940/1‑149 (MQ=255)
                        cTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCaa                                                                                                                            >  1:656702/1‑148 (MQ=255)
                               ccGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAttactt                                                                                                                     >  1:593785/1‑148 (MQ=255)
                                    aGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGcc                                                                                                               >  4:258011/1‑149 (MQ=255)
                                       gcCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAAtttt                                                                                                                                    >  1:551430/1‑125 (MQ=255)
                                       gcCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAAtttt                                                                                                                                    <  2:551430/125‑1 (MQ=255)
                                         cTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAAc                                                                                                          >  3:271492/1‑149 (MQ=255)
                                         cTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGACTAAAc                                                                                                          >  4:369996/1‑149 (MQ=255)
                                          ttAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAAcc                                                                                                         >  3:72099/1‑149 (MQ=255)
                                           tAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAAcc                                                                                                         >  1:783482/1‑148 (MQ=255)
                                           tAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAAcc                                                                                                         <  2:154709/148‑1 (MQ=255)
                                              cGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCtttt                                                                                                     <  3:175061/149‑1 (MQ=255)
                                                ccGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCtttttt                                                                                                   <  3:388238/149‑1 (MQ=255)
                                                 cGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTCCTTACCGCCTAAACCtttttt                                                                                                   >  1:128107/1‑148 (MQ=255)
                                                 cGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTtc                                                                                                  <  4:769786/149‑1 (MQ=255)
                                                   ggAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCtttttt                                                                                                   <  4:332020/146‑1 (MQ=255)
                                                   ggAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCtttttt                                                                                                   >  3:332020/1‑146 (MQ=255)
                                                   ggAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCttt                                                                                                      >  2:961555/1‑143 (MQ=255)
                                                   ggAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCttt                                                                                                      <  1:961555/143‑1 (MQ=255)
                                                   ggAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTtct                                                                                                 <  2:47967/148‑1 (MQ=255)
                                                   ggAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTtct                                                                                                 <  1:734548/148‑1 (MQ=255)
                                                    gAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTCCCGCCTAAACCTTTTTtctc                                                                                                >  2:281368/1‑148 (MQ=255)
                                                    gAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTtctc                                                                                                >  2:23980/1‑148 (MQ=255)
                                                     aaaCGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCAACATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTcc                                                                                               >  1:200482/1‑148 (MQ=255)
                                                      aaCGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAg                                                                                             >  3:318902/1‑149 (MQ=255)
                                                          ttttACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGttct                                                                                         >  3:498026/1‑149 (MQ=255)
                                                          ttttACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGttc                                                                                          >  1:682603/1‑148 (MQ=255)
                                                          ttttACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGttc                                                                                          >  1:657014/1‑148 (MQ=255)
                                                             tACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGttcttc                                                                                       <  1:529027/148‑1 (MQ=255)
                                                              aCCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTtcttct                                                                                      <  2:657014/148‑1 (MQ=255)
                                                              aCCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTcttctt                                                                                     <  3:258011/149‑1 (MQ=255)
                                                                  aTGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTaaa                                                                                 <  4:81091/149‑1 (MQ=255)
                                                                  aTGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTaaa                                                                                 <  3:192409/149‑1 (MQ=255)
                                                                   tGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAg                                                                                <  4:664787/149‑1 (MQ=255)
                                                                     ccGCGAAAAGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAAATTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGt                                                                               <  2:792080/148‑1 (MQ=255)
                                                                      cgcgAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGtt                                                                              >  1:702359/1‑148 (MQ=255)
                                                                       gcgAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGt                                                                            >  4:812187/1‑149 (MQ=255)
                                                                        cgAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGt                                                                            <  2:682603/148‑1 (MQ=255)
                                                                              gagagaTACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGAGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCAcgcg                                                                      <  1:514806/148‑1 (MQ=255)
                                                                                  gaTACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGAt                                                                  <  1:544672/148‑1 (MQ=255)
                                                                                           tAGACCGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACg                                                        >  3:323680/1‑149 (MQ=255)
                                                                                               cTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCgg                                                    <  3:203784/149‑1 (MQ=255)
                                                                                               cTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCgg                                                    >  4:865677/1‑149 (MQ=255)
                                                                                                tGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGt                                                   >  3:779658/1‑149 (MQ=255)
                                                                                                  ttGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTc                                                                           <  3:804666/123‑1 (MQ=255)
                                                                                                  ttGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTATCCAGTTCTTCTTTAAAGTTGTc                                                                           >  4:804666/1‑123 (MQ=255)
                                                                                                       tAACGTCTCCGTGAATTACTGGGCGTTCACACCCCATCTGCATCATGCAGAGGGTCAATTTTCCGGCAAATTACTTAACAGCTAAACCTTTTGTCACCATTTATTCTTTAAAGTTGTCACGCGTGATCAATACCACGTCGTTAAcata                                             >  2:571947/1‑145 (MQ=255)
                                                                                                       tAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCg                                            <  4:318902/149‑1 (MQ=255)
                                                                                                        aaCGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCg                                            >  1:455970/1‑148 (MQ=255)
                                                                                                               ccGTGAATCACTGGTCGTTCACACTCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAAtt                                     >  1:1421/1‑148 (MQ=255)
                                                                                                                cGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAAtttt                                   >  3:696771/1‑149 (MQ=255)
                                                                                                                   gAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGc                                <  3:540086/149‑1 (MQ=255)
                                                                                                                     aTCACTGGTCGTTCACACCCCCTCTGCAACATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCg                               <  2:200482/148‑1 (MQ=255)
                                                                                                                      tCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGt                             >  3:386316/1‑149 (MQ=255)
                                                                                                                      tCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGt                             >  4:277625/1‑149 (MQ=255)
                                                                                                                          tGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCaa                         <  3:39852/149‑1 (MQ=255)
                                                                                                                          tGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCa                          >  2:832304/1‑148 (MQ=255)
                                                                                                                           ggTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCaa                         >  1:379289/1‑148 (MQ=255)
                                                                                                                           ggTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCaa                         <  2:656702/148‑1 (MQ=255)
                                                                                                                               gTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCt                    >  4:858796/1‑149 (MQ=255)
                                                                                                                                ttCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCt                    >  2:829843/1‑148 (MQ=255)
                                                                                                                                ttCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGACAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCtt                   >  4:200875/1‑149 (MQ=255)
                                                                                                                                 tCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCttt                  <  4:386316/149‑1 (MQ=255)
                                                                                                                                 tCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCtt                   <  2:379289/148‑1 (MQ=255)
                                                                                                                                   acacCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTg                >  3:829832/1‑149 (MQ=255)
                                                                                                                                     ttccccTCTGCATGATGCAGAGGGGGATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCt              >  4:830087/3‑149 (MQ=255)
                                                                                                                                     acCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCt              >  4:928807/1‑149 (MQ=255)
                                                                                                                                      cccccTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCt              >  2:875267/1‑148 (MQ=255)
                                                                                                                                       ccccTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTa             <  1:281368/148‑1 (MQ=255)
                                                                                                                                        cccTCTGCATGATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTAcc           >  3:906976/1‑149 (MQ=255)
                                                                                                                                        cccTCTGCATGATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTAcc           >  3:48541/1‑149 (MQ=255)
                                                                                                                                        cccTCTGCATGATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTAcc           >  3:183930/1‑149 (MQ=255)
                                                                                                                                        cccTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTAcc           >  4:526551/1‑149 (MQ=255)
                                                                                                                                        cccTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTAcc           >  4:618408/1‑149 (MQ=255)
                                                                                                                                        cccTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTAc            >  1:414267/1‑148 (MQ=255)
                                                                                                                                         ccTCTGCATGATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAAtttt                                   <  2:917073/124‑1 (MQ=255)
                                                                                                                                         ccTCTGCATGATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAAtttt                                   >  1:917073/1‑124 (MQ=255)
                                                                                                                                         ccTCTGCATGATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTAccc          >  4:93189/1‑149 (MQ=255)
                                                                                                                                         ccTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTAcc           >  2:59843/1‑148 (MQ=255)
                                                                                                                                          ctctGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTAccc          >  1:378484/1‑148 (MQ=255)
                                                                                                                                             tGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGtt      <  3:812187/149‑1 (MQ=255)
                                                                                                                                             tGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGt       <  2:1421/148‑1 (MQ=255)
                                                                                                                                               catcatGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTg     <  2:414267/148‑1 (MQ=255)
                                                                                                                                                atcatGCAGAGGGGAATTTTGCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTa   >  4:864607/1‑149 (MQ=255)
                                                                                                                                                 tcatGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGCAACTTCGGTAAATTTTCGCGGTTCAACGTCTTTTGCTACCCAGTTGTaa  >  3:191477/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ACCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTAA  >  NC_000913/1984966‑1985259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: