Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,187,314 C→G 5.3% intergenic (+16/+66) rcnB → / ← yehA periplasmic modulator of Ni and Co efflux/putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,187,3140CG 5.3% 404.3 / 11.2 114intergenic (+16/+66)rcnB/yehAperiplasmic modulator of Ni and Co efflux/putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (56/51);  new base G (3/3);  total (59/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

Only 100 of 118 total aligned reads displayed.

AGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGACGTAGTAACTG  >  NC_000913/2187168‑2187461
                                                                                                                                                  |                                                                                                                                                   
aGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCtcatca                                                                                                                                                    <  2:570094/148‑1 (MQ=255)
  ttGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATg                                                                                                                                                  <  2:596062/148‑1 (MQ=255)
  ttGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATg                                                                                                                                                  <  2:750231/148‑1 (MQ=255)
    gCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAg                                                                                                                                                <  1:402663/148‑1 (MQ=255)
      gTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAgggg                                                                                                                                             <  3:171712/149‑1 (MQ=255)
       tAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAgggg                                                                                                                                             <  1:195166/148‑1 (MQ=255)
        aaCCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATGATGAgggggc                                                                                                                                           <  4:717423/149‑2 (MQ=255)
        aaCCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGa                                                                                                                                           >  4:503899/1‑149 (MQ=255)
              cccGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATGATGAgggggctttttt                                                                                                                                     <  3:759866/149‑8 (MQ=255)
                    ccTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGacac                                                                                                                                >  1:257283/1‑148 (MQ=255)
                    ccTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGacac                                                                                                                                >  1:724861/1‑148 (MQ=255)
                        aTGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCt                                                                                                                              >  2:77351/1‑146 (MQ=255)
                             gggACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATTAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTAttt                                                                                                                      <  4:778839/149‑1 (MQ=255)
                             gggACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTAtt                                                                                                                       <  1:491323/148‑1 (MQ=255)
                              ggACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTAttt                                                                                                                      <  1:115455/148‑1 (MQ=255)
                              ggACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCACTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTAtttt                                                                                                                     <  4:38187/149‑1 (MQ=255)
                               gACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTAtttt                                                                                                                     >  1:362693/1‑148 (MQ=255)
                                aCGCACTGGACCTATCTGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTAtttttt                                                                                                                   <  3:350057/149‑1 (MQ=255)
                                 cGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTtta                                                                                                                  <  3:485948/149‑1 (MQ=255)
                                   cACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAACGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCACCATGAGGGGGAAATGCAGACACCTTGTTAttttttct                                                                                                                 >  2:524230/1‑146 (MQ=255)
                                     cTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTttatta                                                                                                               >  2:899900/1‑148 (MQ=255)
                                           cTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCa                                                                                                        >  3:905699/1‑149 (MQ=255)
                                           cTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCa                                                                                                        >  3:843952/1‑149 (MQ=255)
                                            tataTGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCa                                                                                                        <  2:724861/148‑1 (MQ=255)
                                              tatGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACt                                                                                                      >  1:871858/1‑148 (MQ=255)
                                              tatGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACt                                                                                                      >  2:925405/1‑148 (MQ=255)
                                               atGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTttattatt                                                                                                             >  1:265354/1‑140 (MQ=255)
                                               atGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTttattatt                                                                                                             <  2:265354/140‑1 (MQ=255)
                                                tGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGc                                                                                                                                      <  1:679471/114‑1 (MQ=255)
                                                tGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGc                                                                                                                                      >  2:679471/1‑114 (MQ=255)
                                                    tgacgcgTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGc                                                                                                                                      >  1:833765/4‑110 (MQ=255)
                                                    tGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGc                                                                                                                                      <  2:833765/110‑1 (MQ=255)
                                                     ggCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTc                                                                                              <  3:263789/149‑1 (MQ=255)
                                                        gcgTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTcttg                                                                                           >  4:148096/1‑149 (MQ=255)
                                                        gcgTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTcttg                                                                                           >  4:219193/1‑149 (MQ=255)
                                                         cgTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTAttttt                                                                                                                    <  2:221755/123‑1 (MQ=255)
                                                         cgTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTcttgc                                                                                          <  3:556273/149‑1 (MQ=255)
                                                           tACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTcttgctt                                                                                        <  4:671317/149‑1 (MQ=255)
                                                               tgtTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGtt                                                                                     >  1:34478/1‑148 (MQ=255)
                                                                gtTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGttat                                                                                   >  3:306201/1‑149 (MQ=255)
                                                                gtTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGtta                                                                                    >  1:240379/1‑148 (MQ=255)
                                                                 ttGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAg                                                                                                           <  1:135125/124‑1 (MQ=255)
                                                                 ttGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAg                                                                                                           >  2:135125/1‑124 (MQ=255)
                                                                 ttGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGttat                                                                                   >  1:339459/1‑148 (MQ=255)
                                                                   gATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAg                                                                                >  3:913488/1‑149 (MQ=255)
                                                                   gATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAg                                                                                >  4:347681/1‑149 (MQ=255)
                                                                    aTCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAg                                                                                >  2:71156/1‑148 (MQ=255)
                                                                    aTCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAg                                                                                >  1:340826/1‑148 (MQ=255)
                                                                     tCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTc                                                                              >  4:516694/1‑149 (MQ=255)
                                                                     tCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTc                                                                              <  3:425805/149‑1 (MQ=255)
                                                                      cAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTc                                                                              >  2:474899/1‑148 (MQ=255)
                                                                       aGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGt                                                                            >  4:658956/1‑149 (MQ=255)
                                                                            cacCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTAttt                                                                        >  1:655306/1‑148 (MQ=255)
                                                                             acCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTc                                                                       <  1:292230/148‑1 (MQ=255)
                                                                               cGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCAc                                                                     >  1:676577/1‑148 (MQ=255)
                                                                                    gTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGa                                                                <  2:648189/148‑1 (MQ=255)
                                                                                     tAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGAt                                                               >  2:427469/1‑148 (MQ=255)
                                                                                       aaaTCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTCGCTTGTTATTAGTCGTATTTCACGTTGATTaa                                                            <  4:316395/149‑1 (MQ=255)
                                                                                        aaTCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACGTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAAt                                                           >  4:566116/1‑149 (MQ=255)
                                                                                        aaTCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTaa                                                            <  1:474899/148‑1 (MQ=255)
                                                                                         aTCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATg                                                          >  3:583955/1‑149 (MQ=255)
                                                                                          tCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGc                                                         <  4:104757/149‑1 (MQ=255)
                                                                                          tCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGc                                                         <  3:503899/149‑1 (MQ=255)
                                                                                           cATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGc                                                         >  2:798447/1‑148 (MQ=255)
                                                                                              tAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTAttt                                                                        <  2:330584/130‑1 (MQ=255)
                                                                                              tAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGtt                                                     <  3:525977/149‑1 (MQ=255)
                                                                                              tAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGtt                                                     <  3:632751/149‑1 (MQ=255)
                                                                                                    cTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTcc                                               >  4:543529/1‑149 (MQ=255)
                                                                                                     tACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCa                                              >  3:666590/1‑149 (MQ=255)
                                                                                                      acgacgGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCa                                              >  2:539889/1‑148 (MQ=255)
                                                                                                      acgacgGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAg                                             <  3:148096/149‑1 (MQ=255)
                                                                                                          cgGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTgc                                          <  2:919548/148‑1 (MQ=255)
                                                                                                             tGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTc                                                                              <  4:177469/109‑1 (MQ=255)
                                                                                                             tGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTc                                                                              >  3:177469/1‑109 (MQ=255)
                                                                                                                  ttttttATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGAtt                                  >  2:446487/1‑148 (MQ=255)
                                                                                                                    ttttATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTa                                <  1:899900/148‑1 (MQ=255)
                                                                                                                        atcatcGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACtt                            <  2:719490/148‑1 (MQ=255)
                                                                                                                          catcGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACtttgt                         <  4:832557/149‑1 (MQ=255)
                                                                                                                          atcatcTAAAAAAAGCCCCCTCATCGTGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTGGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACtttgt                         <  3:933799/144‑1 (MQ=255)
                                                                                                                           atcGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACtttgt                         <  2:825246/148‑1 (MQ=255)
                                                                                                                            tcGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACtttgtt                        >  1:30253/1‑148 (MQ=255)
                                                                                                                               cTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTttgtttgt                     <  1:49238/148‑1 (MQ=255)
                                                                                                                               cTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTa                    >  4:832717/1‑149 (MQ=255)
                                                                                                                                tAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAAt                                                           <  3:286768/109‑1 (MQ=255)
                                                                                                                                tAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAAt                                                           >  4:286768/1‑109 (MQ=255)
                                                                                                                                   gcatttcccccTCATGATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCg                 >  1:440395/7‑148 (MQ=255)
                                                                                                                                    aaaaGCCCCCTCACAATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGt                <  1:524230/148‑1 (MQ=255)
                                                                                                                                     aaaGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTa               >  2:412100/1‑148 (MQ=255)
                                                                                                                                      aaGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAg              <  1:446487/148‑1 (MQ=255)
                                                                                                                                       aGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGAc            <  3:832717/149‑1 (MQ=255)
                                                                                                                                       aGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGAc            <  3:743326/149‑1 (MQ=255)
                                                                                                                                         cccccTCATGATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCgg                                                       >  1:149653/1‑104 (MQ=255)
                                                                                                                                         cccccTCATGATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCgg                                                       <  2:149653/104‑1 (MQ=255)
                                                                                                                                          ccccTCATGATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGACgt          >  1:748436/1‑148 (MQ=255)
                                                                                                                                          ccccTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGACgt          >  1:124599/1‑148 (MQ=255)
                                                                                                                                            ccTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGACgtag        <  4:861817/148‑1 (MQ=255)
                                                                                                                                             cTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGACgtagt       >  2:845100/1‑148 (MQ=255)
                                                                                                                                                 tcatGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGt                <  2:189802/135‑1 (MQ=255)
                                                                                                                                                 tcatGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGt                >  1:189802/1‑135 (MQ=255)
                                                                                                                                                  catGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGACGTAGTAACTg  >  1:307459/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGACGTAGTAACTG  >  NC_000913/2187168‑2187461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: