Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,282,832 | C→G | 5.6% | intergenic (+31/+108) | rplY → / ← yejK | 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,282,832 | 0 | C | G | 5.6% | 366.7 / 10.8 | 107 | intergenic (+31/+108) | rplY/yejK | 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein |
Reads supporting (aligned to +/- strand): ref base C (62/39); new base G (2/4); total (64/43) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.17e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01 |
Only 100 of 112 total aligned reads displayed.
TACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGT > NC_000913/2282685‑2282974 | tACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcg > 4:372801/1‑149 (MQ=255) gCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcg‑gcgg > 3:538119/1‑149 (MQ=255) gCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcg‑gcg > 1:749326/1‑148 (MQ=255) gAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCgggg > 4:418997/1‑149 (MQ=255) ttCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCCGGA‑GCGGGGtttt > 4:329409/1‑149 (MQ=255) ttCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCCGGA‑GCGGGGttt < 2:363014/148‑1 (MQ=255) tCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCCGGA‑GCGGGGttttt < 4:664943/149‑1 (MQ=255) tCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGC‑CGGAGCGGGGttttt < 3:329409/149‑1 (MQ=255) gACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGt < 4:682676/149‑1 (MQ=255) aCCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTa > 4:846358/1‑149 (MQ=255) ccATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTAt < 3:418997/149‑1 (MQ=255) ccATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTAt > 3:63199/1‑149 (MQ=255) tCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTg > 4:56558/1‑149 (MQ=255) cGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTg > 1:526209/1‑148 (MQ=255) tgACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGAtt > 1:679476/1‑148 (MQ=255) gACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGGTTTTTGTATCTGCAGATTAt > 3:236296/1‑149 (MQ=255) gACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGC‑CGGAGCGGGGtttt < 3:824024/132‑1 (MQ=255) cGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGc < 4:191305/149‑1 (MQ=255) aaaGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTg > 2:759738/1‑148 (MQ=255) aaaGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGa > 4:728555/1‑149 (MQ=255) aaGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCCCCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGa < 2:844952/148‑1 (MQ=255) gAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTa > 3:335276/1‑149 (MQ=255) aTCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACgg > 3:128101/1‑149 (MQ=255) tCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACgg > 1:130169/1‑148 (MQ=255) cAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTa > 3:948533/1‑149 (MQ=255) aaaGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTAt > 4:831049/1‑149 (MQ=255) aaGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTAt < 1:50153/148‑1 (MQ=255) aaGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTAt < 2:949779/148‑1 (MQ=255) aGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTAtt < 2:749326/148‑1 (MQ=255) ttAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCt > 4:504452/1‑149 (MQ=255) tAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCt > 2:644289/1‑148 (MQ=255) tAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCt > 2:783946/1‑148 (MQ=255) aaaGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAt < 4:895029/149‑1 (MQ=255) aaGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGttttt < 1:128494/111‑1 (MQ=255) aaGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGttttt > 2:128494/1‑111 (MQ=255) aGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAttt > 3:390250/1‑149 (MQ=255) tCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAtttttt < 3:609435/149‑1 (MQ=255) tCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAttttt < 1:183793/148‑1 (MQ=255) cAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAtttttt > 2:938731/1‑148 (MQ=255) aGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcg‑gcg > 4:725901/1‑97 (MQ=255) aGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcg‑gcg < 3:725901/97‑1 (MQ=255) aGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGc < 4:128101/149‑1 (MQ=255) ggACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGc > 1:763564/1‑148 (MQ=255) cGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAgg > 1:594443/1‑148 (MQ=255) cGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATaa < 3:504452/149‑1 (MQ=255) tCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAgg > 4:371091/1‑149 (MQ=255) cACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAgg > 1:818825/1‑148 (MQ=255) aCCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCg > 3:486734/1‑149 (MQ=255) cccGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGt > 3:517026/1‑149 (MQ=255) ccGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGtt > 3:779968/1‑149 (MQ=255) aCAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcg > 4:886753/1‑149 (MQ=255) aaaCCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcgcc > 3:722727/1‑149 (MQ=255) gCTGCAGCACATCGACTTCGTTCGCGCTTAATAGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTgg < 4:323955/149‑1 (MQ=255) tGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAGAACCCCGCTCCG‑TCGGGGTTTTTTGTATCTGCAGATTATGCCTGACTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCa > 4:908569/1‑149 (MQ=255) gcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAATCCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTc < 2:161251/148‑1 (MQ=255) gcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCa > 4:459203/1‑149 (MQ=255) gcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCa < 3:372801/149‑1 (MQ=255) cacaGCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAg > 4:850906/1‑149 (MQ=255) caTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGaa < 4:486734/149‑1 (MQ=255) caTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGaa < 3:846358/149‑1 (MQ=255) caTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGa < 2:379857/148‑1 (MQ=255) caTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACACCGCATCTGGCATTCAGaa < 3:789505/149‑1 (MQ=255) aTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGaag > 3:85748/1‑148 (MQ=255) aTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTAACGCCGCATCTGGCATTCAGaa > 1:247758/1‑148 (MQ=255) tCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACCCCCCATCTGGCATTCAGAac > 2:876324/1‑148 (MQ=255) gACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAacac > 1:688152/1‑148 (MQ=255) tcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGa < 1:783946/148‑1 (MQ=255) tcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGAt > 3:495258/1‑149 (MQ=255) gcTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTaa < 1:938731/148‑1 (MQ=255) tAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTg > 2:591828/1‑148 (MQ=255) aaTTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTgcc < 3:403931/149‑1 (MQ=255) aaTTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTgc < 1:310494/148‑1 (MQ=255) tGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTgccgc > 1:434124/1‑148 (MQ=255) gCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCa > 3:754982/1‑149 (MQ=255) gAATAAGTTGTAAAAAACCCCGCTCCG‑GCTGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGATCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCTGCCAGa < 2:50551/148‑1 (MQ=255) gAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGa < 2:594443/148‑1 (MQ=255) aaTAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGAc > 2:819976/1‑148 (MQ=255) aaTAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACg > 4:151289/1‑149 (MQ=255) aTAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAg < 4:315157/82‑1 (MQ=255) aTAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAg > 3:315157/1‑82 (MQ=255) aTAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGa > 2:386207/1‑88 (MQ=255) aTAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGa < 1:386207/88‑1 (MQ=255) aTAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACCCAAAACCGATTAATTGCCGCCAGACg < 2:818825/148‑1 (MQ=255) aTAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACg < 2:141435/148‑1 (MQ=255) aTAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACg > 1:170446/1‑148 (MQ=255) aTAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGt > 4:79431/1‑149 (MQ=255) tAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGt < 1:287378/148‑1 (MQ=255) tAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTc > 3:844528/1‑149 (MQ=255) tAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTc > 4:634311/1‑149 (MQ=255) ttGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATGCGGCGTGAACGCCTTATCTGGcc > 3:163327/1‑107 (MQ=38) ttGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATGCGGCGTGAACGCCTTATCTGGcc < 4:163327/108‑2 (MQ=38) tGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATGCGGCGTGAACGCCTTATCTGGc > 1:926866/1‑106 (MQ=38) tGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGgc > 2:663899/1‑148 (MQ=255) tAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCt > 4:917664/1‑149 (MQ=255) aaaaaaCCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTg > 4:409824/1‑149 (MQ=255) aaaCCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATaa < 2:857450/79‑1 (MQ=255) aaaCCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATaa > 1:857450/1‑79 (MQ=255) aaaCCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCaa > 4:763246/1‑149 (MQ=255) ccGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTgg > 1:671594/1‑148 (MQ=255) cGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGt > 1:694450/1‑148 (MQ=255) | TACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGT > NC_000913/2282685‑2282974 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |