Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,729,466 T→A 9.6% intergenic (‑22/+150) gltW ← / ← rrsG tRNA‑Glu/16S ribosomal RNA of rrnG operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,729,4660TA 9.6% 159.9 / 8.0 52intergenic (‑22/+150)gltW/rrsGtRNA‑Glu/16S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base T (26/21);  new base A (2/3);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAA  >  NC_000913/2729319‑2729613
                                                                                                                                                   |                                                                                                                                                   
gTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGt                                                                                                                                                     >  2:530278/1‑148 (MQ=35)
  cTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTg                                                                                                                                                   <  2:325450/148‑1 (MQ=33)
    gTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCg                                                                                                                                                 <  1:327599/148‑1 (MQ=32)
    gTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGt                                                                                                                                                <  4:135450/149‑1 (MQ=32)
      tttGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTg                                                                                                                                               >  2:811720/1‑148 (MQ=32)
         gagaTATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCAACGCGTGataa                                                                                                                                           <  3:151974/149‑2 (MQ=12)
         gagaTATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCAACGCGTGata                                                                                                                                            <  1:449947/148‑1 (MQ=12)
         gagaTATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCAACGCGTGata                                                                                                                                            <  2:636909/148‑1 (MQ=12)
             tatTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATAtttt                                                                                                                                        <  2:444222/148‑1 (MQ=32)
               ttGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTGCCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCgt                                                                                                                                     <  4:799768/149‑1 (MQ=17)
                                                                                               gCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTa                                                      >  1:508382/1‑148 (MQ=11)
                                                                                                    gtCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACt                                                <  3:875793/149‑1 (MQ=32)
                                                                                                     tCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACtt                                               >  4:139589/1‑149 (MQ=32)
                                                                                                     tCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACt                                                <  2:437172/148‑1 (MQ=32)
                                                                                                     tCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCATTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACt                                                <  2:336425/148‑1 (MQ=17)
                                                                                                       cTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACtttt                                             >  4:184973/1‑149 (MQ=33)
                                                                                                       cTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACtttt                                             >  4:154964/1‑149 (MQ=32)
                                                                                                       cTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACtttt                                             <  3:622778/149‑1 (MQ=32)
                                                                                                       cTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACtttt                                             >  3:720147/1‑149 (MQ=33)
                                                                                                         gggCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTtca                                           >  4:642239/1‑149 (MQ=34)
                                                                                                          ggCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTtca                                           <  1:144872/148‑1 (MQ=34)
                                                                                                            ccTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTtcatca                                        <  3:199321/149‑1 (MQ=38)
                                                                                                            ccTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTtcatca                                        <  3:12144/149‑1 (MQ=37)
                                                                                                             ctctAGACGAAGGGGACACGAACATTGCTTATCACGCGTTGCGTGATATTTTCGTGTACGGTGAGCTCTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAg                                       >  4:803198/1‑149 (MQ=255)
                                                                                                              tctAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGa                                      >  4:33462/1‑149 (MQ=38)
                                                                                                                  gACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACaa                                   >  2:778093/1‑148 (MQ=39)
                                                                                                                  gACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAAt                                  >  3:831818/1‑149 (MQ=37)
                                                                                                                  gACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAAt                                  <  3:438034/149‑1 (MQ=38)
                                                                                                                  gACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAAt                                  <  4:231234/149‑1 (MQ=37)
                                                                                                                   aCGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAAt                                  >  2:899945/1‑148 (MQ=39)
                                                                                                                     gAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCtg                               >  3:333885/1‑149 (MQ=38)
                                                                                                                      aaGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCtg                               <  1:204170/148‑1 (MQ=37)
                                                                                                                        ggggACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCtgtg                             <  2:128655/148‑1 (MQ=38)
                                                                                                                        ggggACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCtgtg                             >  2:429094/1‑148 (MQ=39)
                                                                                                                         gggACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCtgtgt                            >  2:48883/1‑148 (MQ=38)
                                                                                                                              acGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCa                       >  1:399778/1‑148 (MQ=38)
                                                                                                                               cGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCAc                      >  2:702430/1‑148 (MQ=38)
                                                                                                                                gAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCACTg                    >  3:30176/1‑149 (MQ=38)
                                                                                                                                  aaaTTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCACTGCa                  >  3:91185/1‑149 (MQ=40)
                                                                                                                                    aTTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCACTGCaa                 <  1:530592/148‑1 (MQ=255)
                                                                                                                                      aaaatATCACGCAACGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATCGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGGGTGAGCACTGCAAAg               >  2:282623/5‑148 (MQ=14)
                                                                                                                                       gCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCACTGCAAAGt              <  1:344515/148‑1 (MQ=39)
                                                                                                                                         ttATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACg           >  3:669809/1‑149 (MQ=40)
                                                                                                                                         ttATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACg           >  4:445886/1‑149 (MQ=40)
                                                                                                                                         atATCACGCAACGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTAc            >  2:51958/2‑148 (MQ=255)
                                                                                                                                          tATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACg           >  2:769815/1‑148 (MQ=40)
                                                                                                                                             cACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCACTGCaaa                >  4:501965/1‑140 (MQ=255)
                                                                                                                                             cACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCACTGCaaa                <  3:501965/140‑1 (MQ=40)
                                                                                                                                             cACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGctt        >  1:62669/1‑148 (MQ=39)
                                                                                                                                             cACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGctt        >  1:452578/1‑148 (MQ=40)
                                                                                                                                               cgcgTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGcttct      >  1:643533/1‑148 (MQ=255)
                                                                                                                                               cgcgTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGcttct      <  1:462665/148‑1 (MQ=40)
                                                                                                                                                  gTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTta   <  2:643533/148‑1 (MQ=40)
                                                                                                                                                   ttGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTtaa  <  2:62669/148‑1 (MQ=40)
                                                                                                                                                   ttGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTtaa  <  1:587847/148‑1 (MQ=40)
                                                                                                                                                   ttGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTtaa  <  2:149991/148‑1 (MQ=40)
                                                                                                                                                   |                                                                                                                                                   
GTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAA  >  NC_000913/2729319‑2729613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑