Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,758,927 A→G 5.3% intergenic (+71/‑58) alpA → / → yfjI CP4‑57 prophage; DNA‑binding transcriptional activator/CP4‑57 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,758,9270AG 5.3% 350.8 / 6.1 110intergenic (+71/‑58)alpA/yfjICP4‑57 prophage; DNA‑binding transcriptional activator/CP4‑57 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (49/55);  new base G (3/3);  total (52/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

Only 100 of 111 total aligned reads displayed.

ATGCTCGGCGTGAAATCTGTCGGATGGATTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCC  >  NC_000913/2758779‑2759072
                                                                                                                                                    |                                                                                                                                                  
aTGCTCGGCGTGAAATCTGTCGGATGGATTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATa                                                                                                                                                    <  4:678889/149‑1 (MQ=255)
    tCGGCGTGAAATCTGTCGGATGGATTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCgg                                                                                                                                                >  3:818670/1‑149 (MQ=255)
     cGGCGTGAAATCTGTCGGATGGATTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGa                                                                                                                                              <  4:559573/149‑1 (MQ=255)
        cGTGAAATCTGTCGGATGGATTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAAt                                                                                                                                            <  2:334976/148‑1 (MQ=255)
            aaaTCTGTCGGATGGATTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCTATTAATGGCGGaaatagc                                                                                                                                        <  1:471219/148‑6 (MQ=255)
            aaaTCTGTCGGATGGATTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCTATTAATGGCGGaaatagc                                                                                                                                        <  1:324843/148‑6 (MQ=255)
               tCTGTCGGATGGATTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCata                                                                                                                                     >  2:323116/1‑148 (MQ=255)
                     ggatggatTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTa                                                                                                                              >  3:508726/1‑149 (MQ=255)
                      gatggatTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTa                                                                                                                              >  1:120005/1‑148 (MQ=255)
                      gatggatTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTa                                                                                                                              >  2:290704/1‑148 (MQ=255)
                        tggatTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTaaa                                                                                                                            <  2:929547/148‑1 (MQ=255)
                                 gCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCtt                                                                                                                   <  1:323116/148‑1 (MQ=255)
                                   cGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTaaa                                                                                                                            >  3:535220/1‑137 (MQ=255)
                                   cGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTaaa                                                                                                                            <  4:535220/137‑1 (MQ=255)
                                   cGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCttaa                                                                                                                 <  1:712401/148‑1 (MQ=255)
                                   cGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCttaa                                                                                                                 <  1:570072/148‑1 (MQ=255)
                                    gagaTTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCttaatt                                                                                                               >  3:18647/1‑149 (MQ=255)
                                    gagaTTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCttaat                                                                                                                <  1:75367/148‑1 (MQ=255)
                                      gaTTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCttaattta                                                                                                             >  3:500849/1‑149 (MQ=255)
                                       aTTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCttaatttaa                                                                                                            <  3:895685/149‑1 (MQ=255)
                                        ttGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCttaatttaat                                                                                                           <  3:47992/149‑1 (MQ=255)
                                        ttGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCttaatttaa                                                                                                            <  1:195170/148‑1 (MQ=255)
                                                tGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGc                                                                                                                                >  3:519920/1‑120 (MQ=255)
                                                tGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGc                                                                                                                                <  4:519920/120‑1 (MQ=255)
                                                tGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATaa                                                                                                    >  1:739017/1‑148 (MQ=255)
                                                 ggTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAaga                                                                                                  <  3:70574/149‑1 (MQ=255)
                                                 ggTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAag                                                                                                   <  1:697843/148‑1 (MQ=255)
                                                  gTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAagag                                                                                                 >  4:676941/1‑149 (MQ=255)
                                                  gTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAagag                                                                                                 <  3:924665/149‑1 (MQ=255)
                                                       cacaACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCAt                                                                                             <  2:823567/148‑1 (MQ=255)
                                                         caACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCAt                                                                                             >  1:685606/1‑146 (MQ=255)
                                                         caACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCAt                                                                                             <  2:685606/146‑1 (MQ=255)
                                                          aaCGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGc                                                                                          >  1:242255/1‑148 (MQ=255)
                                                          aaCGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCa                                                                                         >  4:718796/1‑149 (MQ=255)
                                                             gCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGt                                                                                      >  4:41485/1‑149 (MQ=255)
                                                              cTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGtt                                                                                     <  4:500849/149‑1 (MQ=255)
                                                               tGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTc                                                                                    >  3:605611/1‑149 (MQ=255)
                                                                gTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCa                                                                                   >  3:208079/1‑149 (MQ=255)
                                                                 tAAACTTATGTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCTCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCa                                                                                   <  2:196054/148‑1 (MQ=255)
                                                                  aaaCTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAAt                                                                                 <  3:460817/149‑1 (MQ=255)
                                                                     cTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAg                                                                                                                                                   >  4:296913/1‑81 (MQ=255)
                                                                     cTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAg                                                                                                                                                   <  3:296913/81‑1 (MQ=255)
                                                                      ttATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGgt                                                                              <  1:85502/148‑1 (MQ=255)
                                                                          ttGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTGAATGgtcgtc                                                                          <  1:506038/148‑1 (MQ=255)
                                                                          ttGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTcc                                                                         <  4:545404/149‑1 (MQ=255)
                                                                           tGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCt                                                                        <  3:360220/149‑1 (MQ=255)
                                                                           tGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTAAATGGTCGTCCt                                                                        <  4:713685/149‑1 (MQ=255)
                                                                               ggTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTcc                                                                    <  3:62158/149‑1 (MQ=255)
                                                                                      cATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTcc                                                                    <  3:208180/142‑1 (MQ=255)
                                                                                      cATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTcc                                                                    >  4:208180/1‑142 (MQ=255)
                                                                                      cATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAg                                                              <  2:657841/148‑1 (MQ=255)
                                                                                       aTGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGGATGTTCAATGGTCGTCCTTTCCCTGTAGAt                                                            >  3:776937/1‑149 (MQ=255)
                                                                                        tGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGAt                                                            >  1:107138/1‑148 (MQ=255)
                                                                                         gAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATg                                                           >  2:842836/1‑148 (MQ=255)
                                                                                               aTACTCAATTGCTGTCATCGGAGTGTAAACATTCTGCTATTTCCACCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATCTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTcc                                                    <  3:103633/149‑1 (MQ=255)
                                                                                               aTACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTcc                                                    <  4:618014/149‑1 (MQ=255)
                                                                                                 aCTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTa                                                  >  3:536376/1‑149 (MQ=255)
                                                                                                  cTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTaa                                                 <  3:914367/149‑1 (MQ=255)
                                                                                                   tCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAAtt                                                                                                                       <  4:804886/78‑1 (MQ=255)
                                                                                                   tCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAAtt                                                                                                                       >  3:804886/1‑78 (MQ=255)
                                                                                                   tCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAAGTTAATTAAGTAAGAGGCCTTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTaaa                                                >  4:775011/1‑149 (MQ=255)
                                                                                                    cAATTGTTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTaaa                                                >  1:839621/1‑148 (MQ=255)
                                                                                                    cAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTaaa                                                >  1:836953/1‑148 (MQ=255)
                                                                                                    cAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTaaa                                                >  1:553798/1‑148 (MQ=255)
                                                                                                     aaTTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTaaa                                                                                                       >  3:2318/1‑92 (MQ=255)
                                                                                                      aTTGCTGTCATCGGAGTGTAAACATCCTACTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTGTTAATTTAATTAAATAAGAGGCCTTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCAGTTCCTAAAAtt                                             >  4:910020/1‑149 (MQ=255)
                                                                                                         gCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATc                                          <  3:676941/149‑1 (MQ=255)
                                                                                                          cTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATc                                          <  1:207265/148‑1 (MQ=255)
                                                                                                          cTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCACTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATc                                          >  1:295805/1‑148 (MQ=255)
                                                                                                           tGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAg                                        <  3:775011/149‑1 (MQ=255)
                                                                                                            gTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAgg                                       >  3:806545/1‑149 (MQ=255)
                                                                                                              cATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGa                                      >  2:560886/1‑148 (MQ=255)
                                                                                                                tCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAAt                                    >  1:239698/1‑148 (MQ=255)
                                                                                                                tCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATg                                   >  3:613070/1‑149 (MQ=255)
                                                                                                                tCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATg                                   >  3:122850/1‑149 (MQ=255)
                                                                                                                   gAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCa                                 <  2:911504/148‑1 (MQ=255)
                                                                                                                    aGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAAt                               <  3:695807/149‑1 (MQ=255)
                                                                                                                        tAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTAt                           <  4:613070/149‑1 (MQ=255)
                                                                                                                         aaaCATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTCCCCGGTAGATGCATTTCCTAAAATTATCAGGAATGAAATTTAt                           >  1:452170/1‑148 (MQ=255)
                                                                                                                          aaCATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATg                                                           <  2:474943/115‑1 (MQ=255)
                                                                                                                          aaCATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATg                                                           >  1:474943/1‑115 (MQ=255)
                                                                                                                           aCATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGa                         <  2:744402/148‑1 (MQ=255)
                                                                                                                           aCATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGa                         <  2:502925/148‑1 (MQ=255)
                                                                                                                            cATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAg                       <  3:941911/149‑1 (MQ=255)
                                                                                                                                cTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGa                   >  4:78963/1‑149 (MQ=255)
                                                                                                                                 tGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAAt                                                                                 <  1:61398/86‑1 (MQ=255)
                                                                                                                                 tGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAAt                                                                                 >  2:61398/1‑86 (MQ=255)
                                                                                                                                 tGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGaa                  <  3:853075/149‑1 (MQ=255)
                                                                                                                                   gctattCCGCTATTAATGGCGGAAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAAc                 >  3:359249/5‑149 (MQ=255)
                                                                                                                                    tATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCa                                         >  2:343859/1‑123 (MQ=255)
                                                                                                                                     tattCCGCTATTAATGGCGGAAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCaa                                                                                  <  4:7194/80‑1 (MQ=37)
                                                                                                                                     tattCCGCTATTAATGGCGGAAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCaa                                                                                  >  3:7194/3‑82 (MQ=37)
                                                                                                                                     aTTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAg               <  2:49193/148‑1 (MQ=255)
                                                                                                                                     aTTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAg               >  2:88351/1‑148 (MQ=255)
                                                                                                                                        tCCGCTATTAATGGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCAt            >  1:384202/1‑148 (MQ=255)
                                                                                                                                            ccATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGc        >  1:307295/1‑148 (MQ=255)
                                                                                                                                            ccATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCACTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGc        <  2:295805/148‑1 (MQ=255)
                                                                                                                                            ccATTAATAGCGGAATAGCCATATATGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGc        <  1:842836/148‑1 (MQ=255)
                                                                                                                                             cATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCa       >  2:821980/1‑148 (MQ=255)
                                                                                                                                                tAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCGTACGCAGGc    >  1:4797/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATGCTCGGCGTGAAATCTGTCGGATGGATTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCC  >  NC_000913/2758779‑2759072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: