Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,767,667 T→G 6.7% intergenic (+312/‑43) rnlB → / → yfjP CP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; 50S ribosome‑binding GTPase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,767,6670TG 6.7% 431.3 / 17.8 129intergenic (+312/‑43)rnlB/yfjPCP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
Reads supporting (aligned to +/- strand):  ref base T (53/67);  new base G (6/3);  total (59/70)
Fisher's exact test for biased strand distribution p-value = 2.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 130 total aligned reads displayed.

AATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTAC  >  NC_000913/2767520‑2767814
                                                                                                                                                   |                                                                                                                                                   
aataatGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCCGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTc                                                                                                                                                    >  3:514571/1‑149 (MQ=255)
  taatGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTcg                                                                                                                                                   >  2:259200/1‑148 (MQ=255)
        cACCAATTTTATGGAGATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAACTCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAGCGGCAGAACCTCGCGGTTc                                                                                                                                             <  2:918875/148‑1 (MQ=255)
         aCCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTg                                                                                                                                           >  3:137924/1‑149 (MQ=255)
          ccAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCGCGAGGTTCTg                                                                                                                                           <  2:260637/148‑1 (MQ=255)
          ccAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGAGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTg                                                                                                                                           >  2:769255/1‑148 (MQ=255)
           cAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCGCGAGGTTCTGc                                                                                                                                          <  2:619864/148‑1 (MQ=255)
                   tGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTg                                                                                                                                  <  1:438434/148‑1 (MQ=255)
                     gAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGttt                                                                                                                               <  4:695086/149‑1 (MQ=255)
                     gAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGttt                                                                                                                               <  4:137924/149‑1 (MQ=255)
                        aTCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACTGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTAc                                                                                                                            >  3:312609/1‑149 (MQ=255)
                            tATGATGTCTTTCCCGGTAAATGTTGCTGTGAGTTCAGAAAGATTCTGCAATCTATTTGCATTGAAAAAATCTTCAGATAGATATCATCCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTACCGTTTTTGTTTTACtttt                                                                                                                        <  3:751074/149‑1 (MQ=255)
                             atgatgCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACtttt                                                                                                                        >  2:960369/1‑148 (MQ=255)
                             atgatgCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACtttt                                                                                                                        >  1:581019/1‑148 (MQ=255)
                             atgatgCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGACAGAACCTCGCGGTTCTGCCGTTTTTGt                                                                                                                                 >  2:599404/1‑139 (MQ=255)
                              tgatgCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTc                                                                                                                       <  1:278483/148‑1 (MQ=255)
                                atgCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCaga                                                                                                                    >  3:720570/1‑149 (MQ=255)
                                 tgCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCaga                                                                                                                    <  2:720545/148‑1 (MQ=255)
                                 tgCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCaga                                                                                                                    <  1:16433/148‑1 (MQ=255)
                                   ccTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAgagga                                                                                                                 <  3:47296/149‑1 (MQ=255)
                                    cTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAgaggag                                                                                                                >  3:874807/1‑149 (MQ=255)
                                     tttCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAgaggag                                                                                                                >  2:214111/1‑148 (MQ=255)
                                      ttCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAgt                                                                                                               <  2:311019/148‑1 (MQ=255)
                                        cccGGTAAATGGTGCTGTGAGTTCAGAAAGATTCCGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAgcggt                                                                                                            <  4:514571/149‑1 (MQ=255)
                                          cGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTACAGAGGAGTGGtga                                                                                                          <  4:903257/149‑1 (MQ=255)
                                           ggTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGtgat                                                                                                         <  4:575265/149‑1 (MQ=255)
                                           ggTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGtga                                                                                                          >  2:401872/1‑148 (MQ=255)
                                            gTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAgtggtgtt                                                                                                         >  2:281878/1‑146 (MQ=255)
                                             tAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGtgatg                                                                                                        <  1:335662/148‑1 (MQ=255)
                                                     gCTGTGAGTTCAGAATGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGACCAATCCGATGAACGGAAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAAtttt                                                                                               <  4:377343/149‑1 (MQ=255)
                                                     gCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAAtttt                                                                                               <  4:350308/149‑1 (MQ=255)
                                                     gCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAAtttt                                                                                               <  3:810035/149‑1 (MQ=255)
                                                        gtgAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGaa                                                                                            <  3:646465/149‑1 (MQ=255)
                                                        gtgAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGaa                                                                                            <  3:759966/149‑1 (MQ=255)
                                                        gtgAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGa                                                                                             <  1:453146/148‑1 (MQ=255)
                                                             ttCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCt                                                                                       >  4:512298/1‑149 (MQ=255)
                                                                 gAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGACGAAAAATTTTGAAGTTCTGCa                                                                                    >  1:652063/1‑148 (MQ=255)
                                                                  aaaGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAg                                                                                   >  2:526018/1‑148 (MQ=255)
                                                                  aaaGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAg                                                                                   >  2:14503/1‑148 (MQ=255)
                                                                  aaaGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCGCGAGGTTCTg                                                                                                                                           >  1:217153/1‑92 (MQ=255)
                                                                   aaGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGcc                                                                                 >  4:129036/1‑149 (MQ=255)
                                                                   aaGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGc                                                                                  >  2:251177/1‑148 (MQ=255)
                                                                     gATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCAc                                                                               <  3:286412/149‑1 (MQ=255)
                                                                        tCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTAc                                                                            >  4:741795/1‑149 (MQ=255)
                                                                          tGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAg                                                                          >  3:522443/1‑149 (MQ=255)
                                                                           gCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGtt                                                                                                                                >  2:767063/1‑94 (MQ=255)
                                                                           gCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGtt                                                                                                                                <  1:767063/94‑1 (MQ=255)
                                                                            cAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGa                                                                         >  2:703626/1‑148 (MQ=255)
                                                                            cAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATCCTCCTGAACAATCCGTTGAACGGCATAACCTCGCCGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGa                                                                         <  2:657012/148‑1 (MQ=255)
                                                                               tCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAgt                                                                                                               <  2:512193/107‑1 (MQ=255)
                                                                               tCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAgt                                                                                                               >  1:512193/1‑107 (MQ=255)
                                                                               tCTATGTGCAGTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACt                                                                      >  1:505979/1‑148 (MQ=255)
                                                                                  aTGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTccc                                                                   <  1:636174/148‑1 (MQ=255)
                                                                                         ttGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAAtttt                                                                                               <  3:782811/113‑1 (MQ=255)
                                                                                              aaaaTCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTAcc                                                      >  3:723870/1‑149 (MQ=255)
                                                                                                  tCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTa                                                  <  3:741795/149‑1 (MQ=255)
                                                                                                    ttCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGtga                                                                                                          >  3:403273/1‑91 (MQ=255)
                                                                                                    ttCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGtga                                                                                                          <  4:403273/91‑1 (MQ=255)
                                                                                                    ttCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTAt                                                 <  1:343352/148‑1 (MQ=255)
                                                                                                    ttCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCCACCGCTAt                                                 <  1:14503/148‑1 (MQ=255)
                                                                                                     tCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTTGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATgg                                               <  4:369595/149‑1 (MQ=255)
                                                                                                         atagataTCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTa                                            <  1:526018/148‑1 (MQ=255)
                                                                                                         atagataTCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTAt                                           >  3:874364/1‑149 (MQ=255)
                                                                                                          tagataTCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTAt                                           >  1:273505/1‑148 (MQ=255)
                                                                                                               atCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGaa                                      <  2:276476/148‑1 (MQ=255)
                                                                                                               atCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTCCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGaa                                      <  1:281878/148‑1 (MQ=255)
                                                                                                                tCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAAc                                     >  2:912263/1‑148 (MQ=255)
                                                                                                                   ttCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTCTGGGTATCTGAACGtat                                 >  3:139276/1‑149 (MQ=255)
                                                                                                                   ttCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATgg                                               >  3:158559/1‑135 (MQ=255)
                                                                                                                     ctcCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGtata                                <  1:251177/148‑1 (MQ=255)
                                                                                                                      tcCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAAc                                     >  3:800071/1‑142 (MQ=255)
                                                                                                                      tcCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAAc                                     <  4:800071/142‑1 (MQ=255)
                                                                                                                        cTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgc                            >  3:601470/1‑149 (MQ=255)
                                                                                                                         tGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgca                           >  4:893356/1‑149 (MQ=255)
                                                                                                                         tGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgc                            <  1:912263/148‑1 (MQ=255)
                                                                                                                           aaCAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgcagc                         >  4:55195/1‑149 (MQ=255)
                                                                                                                           aaCAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgcagc                         >  4:864679/1‑149 (MQ=255)
                                                                                                                            aCAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgcagca                        >  3:790868/1‑149 (MQ=255)
                                                                                                                            aCAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgcagc                         <  2:408791/148‑1 (MQ=255)
                                                                                                                              aaTCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGcagcag                       <  1:703626/148‑1 (MQ=255)
                                                                                                                               aTCCGTTGAACGGCAGAACCTCGCGGTTCTGCTGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGAt                     <  4:790868/149‑1 (MQ=255)
                                                                                                                                tCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATa                    >  4:889356/1‑149 (MQ=255)
                                                                                                                                tCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATa                    >  3:43171/1‑149 (MQ=255)
                                                                                                                                 ccGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGc                                                                                     >  2:406480/1‑83 (MQ=255)
                                                                                                                                   gATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATaaa                  <  2:581019/148‑1 (MQ=255)
                                                                                                                                    aTGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAAt                 >  1:875096/1‑148 (MQ=255)
                                                                                                                                      gAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCa               <  1:618279/148‑1 (MQ=255)
                                                                                                                                       aaCGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAg              <  2:295566/148‑1 (MQ=255)
                                                                                                                                        aCGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAgc             <  1:401872/148‑2 (MQ=255)
                                                                                                                                        aCGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGt             >  2:112621/1‑148 (MQ=255)
                                                                                                                                        aCGGCAGAACCGCGAGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGtt            >  3:183544/1‑149 (MQ=255)
                                                                                                                                          ggCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTa           <  1:452492/148‑1 (MQ=255)
                                                                                                                                          ggCAGAACCGCGAGGGTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTaa          >  4:107661/1‑149 (MQ=255)
                                                                                                                                           gCAGAACCGCGAGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAAc         >  4:360396/1‑149 (MQ=255)
                                                                                                                                           gCAGAACCGCGAGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAAc         >  4:310998/1‑149 (MQ=255)
                                                                                                                                             aGAACCGCGAGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAAccc       >  3:895848/1‑149 (MQ=255)
                                                                                                                                              gAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCa      >  3:508681/1‑149 (MQ=255)
                                                                                                                                               aaCCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCAc     <  4:685506/149‑1 (MQ=255)
                                                                                                                                                aCCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACt    <  4:43171/149‑1 (MQ=255)
                                                                                                                                                  cTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTAc  >  3:308744/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTAC  >  NC_000913/2767520‑2767814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: