Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,771,180 G→T 6.3% L11L (CTG→CTT)  yfjT → CP4‑57 prophage; putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,771,1800GT 6.3% 325.8 / 10.1 96L11L (CTG→CTT) yfjTCP4‑57 prophage; putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (39/51);  new base T (2/4);  total (41/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.82e-01

CCGTATACGAAAGAAAAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACG  >  NC_000913/2771032‑2771328
                                                                                                                                                    |                                                                                                                                                    
ccGTATACGAAAGAAAAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTg                                                                                                                                                      <  3:228137/149‑1 (MQ=255)
  gTATACGAAAGAAAAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTgg                                                                                                                                                     <  1:317533/148‑1 (MQ=255)
     taCGAAAGAAAAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCGGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGCTTGTACTGGCaac                                                                                                                                                 <  4:827195/149‑2 (MQ=255)
        gaaagaaaAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTa                                                                                                                                               <  2:581948/148‑1 (MQ=255)
            gaaaAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTTGCCAGTACaaa                                                                                                                                           <  2:479770/148‑2 (MQ=255)
            gaaaAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAAt                                                                                                                                           >  2:306869/1‑148 (MQ=255)
               aaaTACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCTTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGtt                                                                                                                                        <  2:758847/148‑1 (MQ=255)
               aaaTACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGtt                                                                                                                                        >  1:739767/1‑148 (MQ=255)
                aaTACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTTGCCAGTACaaaccggc                                                                                                                                      <  3:150148/149‑7 (MQ=255)
                    cTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCa                                                                                                                                   >  1:106682/1‑148 (MQ=255)
                     ttCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGc                                                                                                                                 >  4:562691/1‑149 (MQ=255)
                      tCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCATCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCt                                                                                                                                <  3:188026/149‑1 (MQ=255)
                        gAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTTGCCAGTACaaac                                                                                                                                          <  4:863382/137‑3 (MQ=255)
                        gAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTTGCCAGTACaaac                                                                                                                                          >  3:863382/1‑135 (MQ=255)
                                 cATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCctc                                                                                                                      >  2:508338/1‑148 (MQ=255)
                                    gACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCg                                                                                                                  <  4:574416/149‑1 (MQ=255)
                                        gCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTcc                                                                                                              >  3:620262/1‑149 (MQ=255)
                                          aaaCTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTccc                                                                                                             <  1:20419/148‑1 (MQ=255)
                                              tGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGa                                                                                                         <  2:106682/148‑1 (MQ=255)
                                               gATGGGGCGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGAc                                                                                                        >  2:517903/1‑148 (MQ=255)
                                                 tGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGActc                                                                                                      <  1:296322/148‑1 (MQ=255)
                                                  gggACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGActct                                                                                                     <  2:278685/148‑1 (MQ=255)
                                                   ggACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTg                                                                                                    <  1:714477/148‑1 (MQ=255)
                                                    gACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTgg                                                                                                   <  1:755239/148‑1 (MQ=255)
                                                         ctgctgATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAg                                                                                             <  3:322372/149‑1 (MQ=255)
                                                         ctgctgATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAg                                                                                             <  3:848000/149‑1 (MQ=255)
                                                           gctgATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATTTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAggg                                                                                           <  4:465076/149‑1 (MQ=255)
                                                           gctgATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAggg                                                                                           <  4:872889/149‑1 (MQ=255)
                                                             ttaTACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGt                                                                                         >  4:459592/3‑149 (MQ=255)
                                                                    gTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCa                                                                                  >  4:797317/1‑149 (MQ=255)
                                                                     tCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAg                                                                                 <  4:751061/149‑1 (MQ=255)
                                                                     tCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAg                                                                                 >  3:948297/1‑149 (MQ=255)
                                                                      cAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAgg                                                                                <  3:375298/149‑1 (MQ=255)
                                                                          cccTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCaa                                                                            >  4:580054/1‑149 (MQ=255)
                                                                          cccTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCa                                                                             <  2:341209/148‑1 (MQ=255)
                                                                           ccTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCaa                                                                            >  2:875367/1‑148 (MQ=255)
                                                                             tGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGc                                                                         >  3:811088/1‑149 (MQ=255)
                                                                              gATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCg                                                                        <  3:413091/149‑1 (MQ=255)
                                                                               aTAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCg                                                                        >  2:262207/1‑148 (MQ=255)
                                                                                tAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGACAATATGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGa                                                                      >  3:862620/1‑149 (MQ=255)
                                                                                   ccGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAAt                                                                    <  2:831950/148‑1 (MQ=255)
                                                                                   ccGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAAt                                                                    <  1:875367/148‑1 (MQ=255)
                                                                                     gggAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGActct                                                                                                     <  3:934193/113‑1 (MQ=255)
                                                                                     gggAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGActct                                                                                                     >  4:934193/1‑113 (MQ=255)
                                                                                      ggAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACa                                                                 <  1:84294/148‑1 (MQ=255)
                                                                                      ggAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAg                                                                <  4:626672/149‑1 (MQ=255)
                                                                                       gAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAgt                                                               <  4:811088/149‑1 (MQ=255)
                                                                                           aaaaaCAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGATTACAGtgtg                                                            >  2:964690/1‑148 (MQ=255)
                                                                                           aaaaaCAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGa                                                           <  3:747900/149‑1 (MQ=255)
                                                                                              aacaacGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACgg                                                        >  3:816208/1‑149 (MQ=255)
                                                                                              aacaacGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACg                                                         >  2:114796/1‑148 (MQ=255)
                                                                                               acaacGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGa                                                       <  3:718905/149‑1 (MQ=255)
                                                                                                 aacGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATg                                                     >  4:690747/1‑149 (MQ=255)
                                                                                                      gaAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGc                                                >  3:799376/1‑149 (MQ=255)
                                                                                                      gaAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGc                                                >  4:548824/1‑149 (MQ=255)
                                                                                                        aaGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCg                                               >  1:274773/1‑148 (MQ=255)
                                                                                                           gagaCATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGaa                                            <  1:381670/148‑1 (MQ=255)
                                                                                                              aCATAAATTAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGCCTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGAt                                        <  4:112457/149‑1 (MQ=255)
                                                                                                              aCATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGAt                                        <  3:757944/149‑1 (MQ=255)
                                                                                                              aCATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGCGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGAt                                        <  3:719199/149‑1 (MQ=255)
                                                                                                                aTAAATGAAAATACTTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATg                                       <  1:94567/148‑1 (MQ=255)
                                                                                                                aTAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGt                                      >  3:626847/1‑149 (MQ=255)
                                                                                                                 tAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGTGTACGGTCAGGGCAATGCGGAATACATTGTGACGGATGCCAGCGGAAAgttgtt                                     >  4:737672/1‑149 (MQ=255)
                                                                                                                  aaaTGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGttt                                    <  4:734593/149‑1 (MQ=255)
                                                                                                                    aTGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTAc                                  >  3:556388/1‑149 (MQ=255)
                                                                                                                    aTGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTAc                                  <  4:799376/149‑1 (MQ=255)
                                                                                                                      gAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCa                                <  3:548824/149‑1 (MQ=255)
                                                                                                                         aaTACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGaaa                                           >  3:164918/1‑135 (MQ=255)
                                                                                                                         aaTACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGaaa                                           <  4:164918/135‑1 (MQ=255)
                                                                                                                         aaTACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATg                                       >  2:232586/1‑139 (MQ=255)
                                                                                                                         aaTACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATg                                       <  1:232586/139‑1 (MQ=255)
                                                                                                                            gccatCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGTACTCTGGCAATAGGGGTACGGTCAGGGCGATGCGGAATACAGTGTGACGGATGCGAGCGGAAAGATGTTTACCGTCAAc                           <  2:841108/144‑1 (MQ=255)
                                                                                                                            aCGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGAAAAGATGTTTACCATCAAc                           <  1:747394/148‑1 (MQ=255)
                                                                                                                              gTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAgt                                                               <  3:34026/110‑1 (MQ=255)
                                                                                                                              gTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAgt                                                               >  4:34026/1‑110 (MQ=255)
                                                                                                                              gTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGc                        >  3:178344/1‑149 (MQ=255)
                                                                                                                                tCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGa                                                       <  1:148207/116‑1 (MQ=255)
                                                                                                                                tCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGa                                                       >  2:148207/1‑116 (MQ=255)
                                                                                                                                  aCTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACg                     <  1:705074/148‑1 (MQ=255)
                                                                                                                                   cTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACgg                    >  1:645708/1‑148 (MQ=255)
                                                                                                                                     aaGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTa                                   <  1:696517/131‑1 (MQ=255)
                                                                                                                                     aaGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTa                                   >  2:696517/1‑131 (MQ=255)
                                                                                                                                      aGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGaa                 <  1:262207/148‑1 (MQ=255)
                                                                                                                                       gCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAAt               <  3:690747/149‑1 (MQ=255)
                                                                                                                                        ccGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATc              <  3:49021/149‑1 (MQ=255)
                                                                                                                                          ggTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATcc             <  2:934838/148‑1 (MQ=255)
                                                                                                                                           gTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTTACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCgg           >  3:739505/1‑149 (MQ=255)
                                                                                                                                           gTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCgg           >  4:942468/1‑149 (MQ=255)
                                                                                                                                            tttGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGa          <  3:942468/149‑1 (MQ=255)
                                                                                                                                            tttGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGa          <  4:178344/149‑1 (MQ=255)
                                                                                                                                            tttGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGGCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCgg           <  2:184044/148‑1 (MQ=255)
                                                                                                                                            attGTACTTGCCAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGa                                                           >  4:605894/2‑100 (MQ=255)
                                                                                                                                            attGTACTTGCCAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGCGGTACGGTCAGGGCAATGCGGAATACAGTGTGa                                                           <  3:605894/99‑1 (MQ=255)
                                                                                                                                                 aCTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGa     >  4:799703/1‑149 (MQ=255)
                                                                                                                                                   tGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCgg                                              >  2:925669/1‑106 (MQ=255)
                                                                                                                                                   tGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCgg                                              <  1:925669/106‑1 (MQ=255)
                                                                                                                                                   tGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAAc   >  4:660586/1‑149 (MQ=255)
                                                                                                                                                   tGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAAc   >  4:5612/1‑149 (MQ=255)
                                                                                                                                                    ggCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACg  >  3:12188/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCGTATACGAAAGAAAAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACG  >  NC_000913/2771032‑2771328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑