Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,020,511 C→G 5.2% intergenic (+22/‑29) ssnA → / → ygfM putative chlorohydrolase/aminohydrolase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,020,5110CG 5.2% 405.0 / 9.4 115intergenic (+22/‑29)ssnA/ygfMputative chlorohydrolase/aminohydrolase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (53/56);  new base G (3/3);  total (56/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 115 total aligned reads displayed.

GCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCC  >  NC_000913/3020365‑3020657
                                                                                                                                                   |                                                                                                                                                  
gCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTGACTTCGATTGCGACTCCAGTTATGCACAAGCCAGAAAAGCCGATGCCAGTATGTGGCGTCGGATGGATGCGGTGGCATAAATGACAGATGCCCTCTTCCCGCAg                                                                                                                                                  >  3:802613/1‑149 (MQ=255)
gCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCa                                                                                                                                                   >  1:492369/1‑148 (MQ=255)
gCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAg                                                                                                                                                  <  4:837804/149‑1 (MQ=255)
    gagggTCAATAGTGTGATGGTCTATGAAGAACGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACATGACAGAGAAGCCGCTGCCAGTATGTGGTGTCGCATCGATGCGCTGGCATACATGACAGATGCCCTCTTCCCGCAGGGaa                                                                                                                                              <  3:307756/146‑1 (MQ=255)
    gATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGa                                                                                                                                               <  1:644585/148‑1 (MQ=255)
    gATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGa                                                                                                                                               >  1:675008/1‑148 (MQ=255)
    gATGGTCAATGGTGTGATGGTCTATGAAGACCATCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCCGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGaa                                                                                                                                              <  4:777195/149‑1 (MQ=255)
       ggTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAagag                                                                                                                                           <  4:2506/149‑1 (MQ=255)
       ggTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAagag                                                                                                                                           <  3:416187/149‑1 (MQ=255)
       ggTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATACTCTCTTCCCTGCGGGAagag                                                                                                                                           <  4:602216/149‑1 (MQ=255)
         tCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAggg                                                                                                                                         <  4:616040/149‑1 (MQ=255)
          cAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAagag                                                                                                                                           <  2:202732/146‑1 (MQ=255)
          cAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAagag                                                                                                                                           >  1:202732/1‑146 (MQ=255)
          cAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAggg                                                                                                                                         >  2:713874/1‑148 (MQ=255)
          cAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAggg                                                                                                                                         <  1:689361/148‑1 (MQ=255)
           aaTGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGtt                                                                                                                                       >  4:622475/1‑149 (MQ=255)
            aTGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGtt                                                                                                                                       >  2:668946/1‑148 (MQ=255)
              ggTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTaaa                                                                                                                                    <  4:708997/149‑1 (MQ=255)
               gtgtGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGAAGCGCTGGCATAAATGACAGATGCCCTCTGCCCGCAGGGAAGAGGGTTAAAt                                                                                                                                   <  4:802613/149‑1 (MQ=255)
                 gtgATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATa                                                                                                                                  <  2:492369/148‑1 (MQ=255)
                 gtgATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATa                                                                                                                                  <  1:567132/148‑1 (MQ=255)
                     tGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGa                                                                                                                              <  1:668946/148‑1 (MQ=255)
                          tATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAaagaag                                                                                                                        >  4:410233/1‑149 (MQ=255)
                          tATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAaagaag                                                                                                                        >  3:102414/1‑149 (MQ=255)
                          tATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCGCTTCCCGCAGGGAAGAGGGTTAAATaa                                                                                                                                 <  3:639428/140‑1 (MQ=255)
                           aTGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAaagaag                                                                                                                        >  2:212435/1‑148 (MQ=255)
                           aTGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAaagaag                                                                                                                        >  1:619929/1‑148 (MQ=255)
                             gAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgatg                                                                                                                     >  4:349511/1‑149 (MQ=255)
                              aaGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgatga                                                                                                                    <  4:265127/149‑1 (MQ=255)
                                gACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgatgat                                                                                                                   <  2:675008/148‑1 (MQ=255)
                                    gTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGaa                                                                                                               <  1:69197/148‑1 (MQ=255)
                                     tCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAAc                                                                                                              >  1:810193/1‑148 (MQ=255)
                                           tAACTTCGATTGCTATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAttttt                                                                                                       <  4:696072/149‑1 (MQ=255)
                                            aaCTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAttttt                                                                                                       <  2:826524/148‑1 (MQ=255)
                                             aCTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCa                                                                                                     <  3:410233/149‑1 (MQ=255)
                                             aCTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCa                                                                                                     >  3:907581/1‑149 (MQ=255)
                                               ttCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAgg                                                                                                   >  3:929438/1‑149 (MQ=255)
                                               ttCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAg                                                                                                    >  2:238030/1‑148 (MQ=255)
                                                    ttGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCg                                                                                               >  2:522873/1‑148 (MQ=255)
                                                     tGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGa                                                                                              <  1:841412/148‑1 (MQ=255)
                                                        gATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGActct                                                                                          >  3:773286/1‑149 (MQ=255)
                                                           tCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGt                                                                                        >  2:819435/1‑148 (MQ=255)
                                                             cATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGaa                                                                                                               <  3:715111/123‑1 (MQ=255)
                                                             cATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGaa                                                                                                               >  4:715111/1‑123 (MQ=255)
                                                              aTTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGaa                                                                                    >  4:257210/1‑149 (MQ=255)
                                                              aTTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGaa                                                                                    >  4:557799/1‑149 (MQ=255)
                                                              aTTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGaa                                                                                    >  3:951680/1‑149 (MQ=255)
                                                              aTTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGa                                                                                     >  1:312309/1‑148 (MQ=255)
                                                               tttATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAAc                                                                                   <  3:726474/149‑1 (MQ=255)
                                                                        aaGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTgg                                                                          >  3:278077/1‑149 (MQ=255)
                                                                        aaGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTg                                                                           <  1:862268/148‑1 (MQ=255)
                                                                         aGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTgg                                                                          >  2:876572/1‑148 (MQ=255)
                                                                          gCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGaa                                                                        >  4:680030/1‑149 (MQ=255)
                                                                             aGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAAc                                                                                   <  4:338350/135‑1 (MQ=255)
                                                                             aGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAAc                                                                                   >  3:338350/1‑135 (MQ=255)
                                                                               aaaaGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGaa                                                                   <  3:349511/149‑1 (MQ=255)
                                                                                     cGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGcgcc                                                              <  2:567514/148‑1 (MQ=255)
                                                                                           cAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCa                                                       <  3:745589/149‑1 (MQ=255)
                                                                                            aGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAg                                                      <  3:157767/149‑1 (MQ=255)
                                                                                            aGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAg                                                      <  3:902157/149‑1 (MQ=255)
                                                                                                  tgGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGa                                                 <  2:29978/148‑1 (MQ=255)
                                                                                                  tgGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGa                                                 <  2:738534/148‑1 (MQ=255)
                                                                                                   ggCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAg                                               >  4:601382/1‑149 (MQ=255)
                                                                                                     cGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGa                                                                                                                              <  2:854636/68‑1 (MQ=255)
                                                                                                     cGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGa                                                                                                                              >  1:854636/1‑68 (MQ=255)
                                                                                                       tCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCg                                            >  1:170679/1‑148 (MQ=255)
                                                                                                        cGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGt                                           <  1:674956/148‑1 (MQ=255)
                                                                                                         ggatggatGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTc                                          <  2:130676/148‑1 (MQ=255)
                                                                                                         ggatggatGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCt                                         >  4:713555/1‑149 (MQ=255)
                                                                                                         ggatggatGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGACGAAGCCGTc                                          <  1:87984/148‑1 (MQ=255)
                                                                                                          gatggatgCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCt                                         >  2:642908/1‑148 (MQ=255)
                                                                                                          gatggatgCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTg                                        <  4:48518/149‑1 (MQ=255)
                                                                                                             ggatgCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGtt                                     <  4:773286/149‑1 (MQ=255)
                                                                                                                   gcTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCgc                               <  4:655181/149‑2 (MQ=255)
                                                                                                                     tGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCgggg                             <  3:622475/149‑1 (MQ=255)
                                                                                                                        cATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGgc                           >  1:438528/1‑148 (MQ=255)
                                                                                                                         aTAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGTAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGgcag                         >  3:136526/1‑149 (MQ=255)
                                                                                                                          tAAATGACAGATGCCCTCTTCCCGCAGGGAAGGGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGgcag                         >  2:866212/1‑148 (MQ=255)
                                                                                                                            aaTGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCaa                      <  3:325295/149‑1 (MQ=255)
                                                                                                                              tGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAAc                                                                                   <  3:25652/86‑1 (MQ=255)
                                                                                                                               gACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACt                   >  3:575028/1‑149 (MQ=255)
                                                                                                                                  aGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCaa                <  3:463627/149‑1 (MQ=255)
                                                                                                                                   gATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCaa                >  2:123647/1‑148 (MQ=255)
                                                                                                                                   gATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGCCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCaa                <  1:212435/148‑1 (MQ=255)
                                                                                                                                    aTGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAAt               <  1:908259/148‑1 (MQ=255)
                                                                                                                                     tGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATg              <  2:730812/148‑1 (MQ=255)
                                                                                                                                      gCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGc             >  1:174207/1‑148 (MQ=255)
                                                                                                                                        ccTCTTCCCTGCGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAttttt                                                                                                       >  4:892203/1‑56 (MQ=25)
                                                                                                                                        ccTCTTCCCTGCGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTac          >  3:443710/1‑149 (MQ=255)
                                                                                                                                        ccTCTTCCCTGCGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAttttt                                                                                                       <  3:892203/56‑1 (MQ=37)
                                                                                                                                        ccTCTTCCCTGCGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCt                                                                            <  2:363153/83‑1 (MQ=39)
                                                                                                                                        ccTCTTCCCTGCGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCt                                                                            >  1:363153/1‑83 (MQ=39)
                                                                                                                                        ccTCTTCCCTGCGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTac          >  4:755121/1‑149 (MQ=255)
                                                                                                                                         ctctTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTaca         >  4:521277/1‑149 (MQ=255)
                                                                                                                                         ctctTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTaca         >  4:255292/1‑149 (MQ=255)
                                                                                                                                         ctctTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTac          <  2:427184/148‑1 (MQ=255)
                                                                                                                                                cGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGtt                                     >  4:396170/1‑114 (MQ=255)
                                                                                                                                                cGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGtt                                     <  3:396170/114‑1 (MQ=255)
                                                                                                                                                cGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAAccc  >  4:654957/1‑149 (MQ=255)
                                                                                                                                                  gCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAAccc  >  2:119813/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCC  >  NC_000913/3020365‑3020657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: