Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,387,553 T→A 7.1% R212R (CGA→CGT aaeB ← p‑hydroxybenzoic acid efflux system component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,387,5530TA 7.1% 435.6 / 19.0 127R212R (CGA→CGTaaeBp‑hydroxybenzoic acid efflux system component
Reads supporting (aligned to +/- strand):  ref base T (47/71);  new base A (3/6);  total (50/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.81e-01

Only 100 of 129 total aligned reads displayed.

CGTATTCTGAATAAGATAAGTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAAGAGCAAATCCG  >  NC_000913/3387406‑3387701
                                                                                                                                                   |                                                                                                                                                    
cgTATTCTGAATAAGATAAGTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGt                                                                                                                                                      >  2:269792/1‑148 (MQ=255)
  tATTCTGAATAAGATAAGTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCg                                                                                                                                                    >  1:432285/1‑148 (MQ=255)
    ttCTGAATAAGATAAGTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCAc                                                                                                                                                 >  3:289883/1‑149 (MQ=255)
     tCTGAATAAGATAAGTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCAc                                                                                                                                                 >  1:387604/1‑148 (MQ=255)
       tGAATAAGATAAGTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCa                                                                                                                                               >  2:370840/1‑148 (MQ=255)
       tGAATAAGATAAGTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCa                                                                                                                                               <  2:432285/148‑1 (MQ=255)
       tGAATAAGATAAGTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAg                                                                                                                                              <  4:140342/149‑1 (MQ=255)
       tGAATAAGATAAGTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGACGCACCa                                                                                                                                               <  2:920131/148‑1 (MQ=255)
         aaTAAGATAAGTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGt                                                                                                                                            >  4:136299/1‑149 (MQ=255)
         aaTAAGATAAGTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGt                                                                                                                                            >  4:251353/1‑149 (MQ=255)
         aaTAAGATAAGTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGt                                                                                                                                            >  4:631837/1‑149 (MQ=255)
         aaTAAGATAAGTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGACGCACCacg                                                                                                                                             <  1:144327/148‑3 (MQ=255)
           taagataagTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGACGCACCacggcg                                                                                                                                          <  3:402423/149‑6 (MQ=255)
              gataagTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGccc                                                                                                                                       >  3:661029/1‑149 (MQ=255)
              gataagTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGccc                                                                                                                                       >  3:699730/1‑149 (MQ=255)
               ataagTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGccc                                                                                                                                       <  2:48503/148‑1 (MQ=255)
                 aagTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCa                                                                                                                                     <  1:30497/148‑1 (MQ=255)
                  agTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAg                                                                                                                                    <  1:451550/148‑1 (MQ=255)
                   gTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTCTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCGTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAgcc                                                                                                                                  >  4:315990/1‑147 (MQ=255)
                       cGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCttt                                                                                                                               >  2:304720/1‑148 (MQ=255)
                        gCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTAt                                                                                                                             >  4:506933/1‑149 (MQ=255)
                            gATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGa                                                                                                                          >  2:492013/1‑148 (MQ=255)
                            gATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGATGCTGCGCATGCTTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGAc                                                                                                                         <  4:699730/149‑1 (MQ=255)
                              ttGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACaa                                                                                                                       <  3:135002/149‑1 (MQ=255)
                                  gTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTc                                                                                                                   >  4:670534/1‑149 (MQ=255)
                                   tAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTc                                                                                                                   <  1:959253/148‑1 (MQ=255)
                                   tAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCa                                                                                                                  >  4:588674/1‑149 (MQ=255)
                                     aTCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCAcc                                                                                                                >  3:715464/1‑149 (MQ=255)
                                                 gATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGTATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCtt                                                                                                    <  3:799088/149‑1 (MQ=255)
                                                 gATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCtt                                                                                                    >  4:101271/1‑149 (MQ=255)
                                                  aTAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTg                                                                                                   <  3:251353/149‑1 (MQ=255)
                                                   tAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTg                                                                                                   <  1:304720/148‑1 (MQ=255)
                                                   tAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTg                                                                                                   <  2:563759/148‑1 (MQ=255)
                                                     gCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATa                                                                                                <  3:269101/149‑1 (MQ=255)
                                                              tCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTg                                                                                        >  2:817950/1‑148 (MQ=255)
                                                               cGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGc                                                                                       >  1:60793/1‑148 (MQ=255)
                                                                gCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGc                                                                                                      <  4:767887/132‑1 (MQ=255)
                                                                gCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGCGCGTCGCACCAGGTCGccc                                                                                                                                       >  3:506242/1‑99 (MQ=255)
                                                                gCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGCGCGTCGCACCAGGTCGccc                                                                                                                                       <  4:506242/99‑1 (MQ=255)
                                                                 cTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACt                                                                                                                     >  2:106598/1‑116 (MQ=255)
                                                                 cTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACt                                                                                                                     <  1:106598/116‑1 (MQ=255)
                                                                  ttttAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTa                                                                                   >  4:937972/1‑149 (MQ=255)
                                                                    ttAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTaa                                                                                  <  2:492060/148‑1 (MQ=255)
                                                                    ttAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTaa                                                                                  <  2:606707/148‑1 (MQ=255)
                                                                    ttAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAAt                                                                                 <  4:562079/149‑1 (MQ=255)
                                                                    ttAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAAt                                                                                 <  3:793981/149‑1 (MQ=255)
                                                                    ttAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAAt                                                                                 <  3:746560/149‑1 (MQ=255)
                                                                       aaCGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGa                                                                              <  3:804212/149‑1 (MQ=255)
                                                                        aCGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGa                                                                              <  2:4162/148‑1 (MQ=255)
                                                                           tCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACa                                                                                                                        >  1:21029/1‑103 (MQ=255)
                                                                           tCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACa                                                                                                                        <  2:21029/103‑1 (MQ=255)
                                                                            cGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATAtt                                                                          <  1:317188/148‑1 (MQ=255)
                                                                             gATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTg                                                                                                   <  3:724697/122‑1 (MQ=255)
                                                                             gATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTg                                                                                                   >  4:724697/1‑122 (MQ=255)
                                                                               ttGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTgcg                                                                       >  2:44955/1‑148 (MQ=255)
                                                                               ttGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGGGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCa                                                                                                         <  1:866374/114‑1 (MQ=255)
                                                                               tcggCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCa                                                                                                         >  2:866374/3‑114 (MQ=255)
                                                                                  gCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGa                                                                    <  1:492013/148‑1 (MQ=255)
                                                                                       cgcCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTTCGCGACCAGCa                                                              <  3:558105/149‑1 (MQ=255)
                                                                                       cgcCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGc                                                               <  1:847174/148‑1 (MQ=255)
                                                                                         cccAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCaaa                                                            <  3:14809/149‑1 (MQ=255)
                                                                                         cccAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCaa                                                             >  2:209486/1‑148 (MQ=255)
                                                                                              cGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACttt                                                        <  2:883755/148‑1 (MQ=255)
                                                                                               gggAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTc                                                       <  1:389978/148‑1 (MQ=255)
                                                                                                ggAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTcc                                                      <  2:797050/148‑1 (MQ=255)
                                                                                                ggAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTcc                                                      <  2:308066/148‑1 (MQ=255)
                                                                                                ggAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCa                                                     <  3:664253/149‑1 (MQ=255)
                                                                                                 gAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCa                                                     >  1:857855/1‑148 (MQ=255)
                                                                                                   aGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGct                                                  <  3:631837/149‑1 (MQ=255)
                                                                                                      ttCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGctct                                                <  2:820715/148‑1 (MQ=255)
                                                                                                       tCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGc                                                               <  2:798354/132‑1 (MQ=255)
                                                                                                       tCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGc                                                               >  1:798354/1‑132 (MQ=255)
                                                                                                        ccATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCg                                              <  1:381825/148‑1 (MQ=255)
                                                                                                          atatTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGAt                                            >  2:463002/1‑148 (MQ=255)
                                                                                                           tatTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTAGATc                                           <  1:516654/148‑1 (MQ=255)
                                                                                                             ttCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGTCTTTATCGACAACTTCACCAGCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCa                                         <  2:367370/148‑1 (MQ=255)
                                                                                                                 ggTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCActtct                                    <  4:753086/149‑1 (MQ=255)
                                                                                                                       tgcGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTg                              >  3:520204/1‑149 (MQ=255)
                                                                                                                       tgcGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTg                              >  4:854236/1‑149 (MQ=255)
                                                                                                                        gcgcATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGctc                                                 >  2:254061/1‑129 (MQ=255)
                                                                                                                         cgtaTGCCTTGTAGTGCCGTGGTGCGTCGCACCAAGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCCTTAATTGATATTGCACGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTgat                            <  4:589074/146‑1 (MQ=255)
                                                                                                                           cATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTAGATCCACTTCTTGTTTga                             >  1:184182/1‑146 (MQ=255)
                                                                                                                           cATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTAGATCCACTTCTTGTTTga                             <  2:184182/146‑1 (MQ=255)
                                                                                                                                cTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGatcgatcg                      >  1:842578/1‑148 (MQ=255)
                                                                                                                                   gTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCgg                   <  1:899065/148‑1 (MQ=255)
                                                                                                                                   gTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGga                  <  3:136299/149‑1 (MQ=255)
                                                                                                                                   gTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGga                  <  3:423192/149‑1 (MQ=255)
                                                                                                                                      gcgcCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGaa               >  4:33947/1‑149 (MQ=255)
                                                                                                                                        gcCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAGCTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGaaaa             <  4:621327/149‑1 (MQ=255)
                                                                                                                                        gcCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGaaaa             <  4:520204/149‑1 (MQ=255)
                                                                                                                                        gcCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGaaa              <  1:380107/148‑1 (MQ=255)
                                                                                                                                         ccGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGctc                                                 >  1:458739/1‑112 (MQ=255)
                                                                                                                                         acctggtgCGACGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGctct                                                >  4:122904/4‑113 (MQ=255)
                                                                                                                                         acctggtgCGACGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGctct                                                <  3:122904/110‑1 (MQ=255)
                                                                                                                                          cGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAag            <  2:531570/148‑1 (MQ=255)
                                                                                                                                           gtggtgCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAagag          <  4:888428/149‑1 (MQ=255)
                                                                                                                                            tggtgCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAAGAGc         <  3:246181/149‑1 (MQ=255)
                                                                                                                                            tggtgCGACGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTaa                                                                                  >  2:355482/1‑76 (MQ=255)
                                                                                                                                               tgCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAAGAGCaaa      <  4:500144/149‑1 (MQ=255)
                                                                                                                                                   tCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAAGAGCAAATCCg  >  3:459922/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGTATTCTGAATAAGATAAGTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAAGAGCAAATCCG  >  NC_000913/3387406‑3387701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: