Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,642,358 T→A 7.5% intergenic (+27/+22) dtpB → / ← rsmJ dipeptide and tripeptide permease B/16S rRNA m(2)G1516 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,642,3580TA 7.5% 309.8 / 11.7 92intergenic (+27/+22)dtpB/rsmJdipeptide and tripeptide permease B/16S rRNA m(2)G1516 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (43/42);  new base A (4/3);  total (47/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

TTGCCCGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTCGCCAGC  >  NC_000913/3642212‑3642506
                                                                                                                                                  |                                                                                                                                                    
ttGCCCGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGtt                                                                                                                                                     >  2:11487/1‑148 (MQ=255)
  gCCCGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTAt                                                                                                                                                   <  2:731143/148‑1 (MQ=255)
    ccGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGc                                                                                                                                                >  3:765047/1‑149 (MQ=255)
     cGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGc                                                                                                                                                >  1:947010/1‑148 (MQ=255)
            aCCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTg                                                                                                                                         >  2:742481/1‑148 (MQ=255)
            aCCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATCGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTg                                                                                                                                         <  2:55218/148‑1 (MQ=255)
             ccgaCGTGTTTGGGAAGATTGGTCTGGTCACACAGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGTCAGAAGGCCATTAATTGTTCGTGCAGAAGACAGGGTAGCGATAACGCTACCCTgc                                                                                                                                        <  1:574891/145‑2 (MQ=255)
             ccAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgt                                                                                                                                        >  1:867204/1‑148 (MQ=255)
              cAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttt                                                                                                                                      <  4:578087/149‑1 (MQ=255)
              cAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttt                                                                                                                                      >  3:66973/1‑149 (MQ=255)
               aaCGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgtttt                                                                                                                                     >  3:279951/1‑149 (MQ=255)
                aCGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgtttta                                                                                                                                    <  4:586468/149‑1 (MQ=255)
                aCGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgtttt                                                                                                                                     <  2:316519/148‑1 (MQ=255)
                aCGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgtttt                                                                                                                                     >  1:88239/1‑148 (MQ=255)
                  gtgtTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttttagt                                                                                                                                  <  4:874723/149‑1 (MQ=255)
                    gtTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGATAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttttagtt                                                                                                                                 >  2:485115/1‑148 (MQ=255)
                    gtTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTACCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttttagtt                                                                                                                                 >  1:395224/1‑148 (MQ=255)
                      ttGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttttagtttta                                                                                                                              <  4:67669/149‑1 (MQ=255)
                       tGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttttagtttta                                                                                                                              <  1:51278/148‑1 (MQ=255)
                       tGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTACCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttttagtttta                                                                                                                              <  2:395224/148‑1 (MQ=255)
                           aaGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACt                                                                                                                          <  1:625634/148‑1 (MQ=255)
                           aaGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACt                                                                                                                          >  1:882233/1‑148 (MQ=255)
                           aaGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACt                                                                                                                          >  1:22328/1‑148 (MQ=255)
                            aGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTgg                                                                                                                        >  3:421683/1‑149 (MQ=255)
                            aGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTg                                                                                                                         >  2:699708/1‑148 (MQ=255)
                               ttGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGATAACGCTAccc                                                                                                                                           <  1:318164/127‑1 (MQ=255)
                               ttGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGATAACGCTAccc                                                                                                                                           >  2:318164/1‑127 (MQ=255)
                                   tctggtcACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTAcc                                                                                                                  <  1:470637/148‑1 (MQ=255)
                                     tggtcACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTg                                                                                                                <  1:11487/148‑1 (MQ=255)
                                      ggtcACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGc                                                                                                               >  2:440120/1‑148 (MQ=255)
                                       gtcACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCAt                                                                                                             >  4:172883/1‑149 (MQ=255)
                                        tcACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATa                                                                                                            >  3:869546/1‑149 (MQ=255)
                                        tcACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATa                                                                                                            >  3:152420/1‑149 (MQ=255)
                                           cGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGa                                                                                                          <  2:427833/148‑1 (MQ=255)
                                              tGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGatat                                                                                                       <  2:720034/148‑1 (MQ=255)
                                               gggCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCa                                                                                                     >  3:335147/1‑149 (MQ=255)
                                                 gCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCaaa                                                                                                   <  3:121059/149‑1 (MQ=255)
                                                   gTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGc                                                                                                 <  4:16686/149‑1 (MQ=255)
                                                      gCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGAt                                                                                              <  4:191795/149‑1 (MQ=255)
                                                       cAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATg                                                                                             <  3:19572/149‑1 (MQ=255)
                                                             tGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACAGGCGTACCTGCATAGATATCAAAGCGATGCCCtt                                                                                        >  1:795318/1‑148 (MQ=255)
                                                              gATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCttt                                                                                       <  2:882233/148‑1 (MQ=255)
                                                                tGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAg                                                                                     <  1:349223/148‑1 (MQ=255)
                                                                tGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGt                                                                                    <  3:448236/149‑1 (MQ=255)
                                                                tGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGt                                                                                    <  3:910284/149‑1 (MQ=255)
                                                                  cTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGa                                                                                  <  4:279951/149‑1 (MQ=255)
                                                                     ttGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTa                                                                               >  3:773569/1‑149 (MQ=255)
                                                                      tGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTAc                                                                              >  4:52745/1‑149 (MQ=255)
                                                                       gATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTAcc                                                                             >  3:470969/1‑149 (MQ=255)
                                                                        aTGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGCTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTAcc                                                                             >  1:828513/1‑148 (MQ=255)
                                                                         tGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACcg                                                                            <  1:666107/148‑1 (MQ=255)
                                                                          ggTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTGTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACcgcg                                                                          >  3:945180/1‑149 (MQ=255)
                                                                            tGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGtt                                                                        <  3:200597/149‑1 (MQ=255)
                                                                            tGCCGTGGCTGAAACGCATGATTGCGACGCCAGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGt                                                                         <  2:22328/148‑1 (MQ=255)
                                                                             gCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGttt                                                                       <  3:381234/149‑1 (MQ=255)
                                                                             gCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGtt                                                                        <  2:297300/148‑1 (MQ=255)
                                                                             gCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGtt                                                                        <  2:357973/148‑1 (MQ=255)
                                                                                gTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTtggc                                                                    >  4:334832/1‑149 (MQ=255)
                                                                                gTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTtggc                                                                    >  3:742139/1‑149 (MQ=255)
                                                                                   gCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTtggcgtg                                                                 <  4:256768/149‑1 (MQ=255)
                                                                                      gAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTtggcgtggcg                                                              >  3:358180/1‑149 (MQ=255)
                                                                                      gAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTtggcgtggcg                                                              >  3:480502/1‑149 (MQ=255)
                                                                                       aaaCGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGa                                                             >  4:839098/1‑149 (MQ=255)
                                                                                       aaaCGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGa                                                             <  4:637242/149‑1 (MQ=255)
                                                                                         aCGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCAGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGAc                                                            >  1:874111/1‑148 (MQ=255)
                                                                                              tGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGc                                                      <  4:941431/149‑1 (MQ=255)
                                                                                                     gACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGt                                               >  3:793556/1‑149 (MQ=255)
                                                                                                      aCGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGt                                               <  2:362639/148‑1 (MQ=255)
                                                                                                                   attaattaTTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCgg                                 >  4:429958/1‑149 (MQ=255)
                                                                                                                    ttaattaTTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCggg                                >  4:521690/1‑149 (MQ=255)
                                                                                                                     taattaTTCTTGCAGAAAGCAAGGTAGCGTTATCGCTACGCTGTTTTAGTTTTCCACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTCACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGgc                               <  3:287678/149‑1 (MQ=255)
                                                                                                                      aattaTTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGgcg                              >  4:297397/1‑149 (MQ=255)
                                                                                                                       attattCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCaa                                                                                                    <  1:292525/78‑1 (MQ=255)
                                                                                                                       attattCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCaa                                                                                                    >  2:292525/1‑78 (MQ=255)
                                                                                                                       attattCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAg                                                                                     <  4:186526/93‑1 (MQ=255)
                                                                                                                       attattCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAg                                                                                     >  3:186526/1‑93 (MQ=255)
                                                                                                                          attCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCttt                          >  4:18250/1‑149 (MQ=255)
                                                                                                                           ttCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCttt                          <  1:440120/148‑1 (MQ=255)
                                                                                                                            tCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTac                        <  4:335147/149‑1 (MQ=255)
                                                                                                                               tGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTacca                      >  2:425475/1‑148 (MQ=255)
                                                                                                                                gCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTaccac                     <  1:742481/148‑1 (MQ=255)
                                                                                                                                  aGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTa                                                                                                                    >  1:227958/1‑51 (MQ=255)
                                                                                                                                  aGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTa                                                                                                                    <  2:227958/51‑1 (MQ=255)
                                                                                                                                  aGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCaaa                                                                                                   <  3:831854/68‑1 (MQ=255)
                                                                                                                                  aGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCaaa                                                                                                   >  4:831854/1‑68 (MQ=255)
                                                                                                                                  aGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACcaccac                  >  4:884219/1‑149 (MQ=255)
                                                                                                                                      aGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGt              >  3:684531/1‑149 (MQ=255)
                                                                                                                                       gCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGtt             >  4:99562/1‑149 (MQ=255)
                                                                                                                                        cAGGGTAGCGATAACGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTac                        <  2:192182/137‑1 (MQ=255)
                                                                                                                                        cAGGGTAGCGATAACGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTac                        >  1:192182/1‑137 (MQ=255)
                                                                                                                                          gggTAGCGATAACGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTg           >  1:562297/1‑148 (MQ=255)
                                                                                                                                           ggTAGCGATAACGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGt         >  4:42472/1‑149 (MQ=255)
                                                                                                                                                cGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTCGcc     <  1:699708/148‑1 (MQ=255)
                                                                                                                                                  ttATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTCGCCAGc  >  4:225156/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TTGCCCGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTCGCCAGC  >  NC_000913/3642212‑3642506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: