Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 283,516 A→C 6.9% N106H (AAT→CAT)  yagF → CP4‑6 prophage; dehydratase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913283,5160AC 6.9% 240.3 / 10.4 72N106H (AAT→CAT) yagFCP4‑6 prophage; dehydratase family protein
Reads supporting (aligned to +/- strand):  ref base A (35/32);  new base C (3/2);  total (38/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

AACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGG  >  NC_000913/283369‑283662
                                                                                                                                                   |                                                                                                                                                  
aacaaGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCaa                                                                                                                                                   >  4:885732/1‑149 (MQ=255)
aacaaGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCaa                                                                                                                                                   >  4:239178/1‑149 (MQ=255)
 acaaGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCaa                                                                                                                                                   >  2:176224/1‑148 (MQ=255)
     gCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCg                                                                                                                                              <  3:522459/149‑1 (MQ=255)
       tCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGGGATCACCCGCAATGGCggg                                                                                                                                            >  4:785571/1‑149 (MQ=255)
        cACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCggg                                                                                                                                            >  2:904501/1‑148 (MQ=255)
        cACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGa                                                                                                                                           >  4:623478/1‑149 (MQ=255)
         aCCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGa                                                                                                                                           <  2:578113/148‑1 (MQ=255)
         aCCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGAt                                                                                                                                          >  4:429573/1‑149 (MQ=255)
         aCCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCAGCGAAGGAGATCACCCGCAATGGCGGGAt                                                                                                                                          <  3:817431/149‑1 (MQ=255)
          ccGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGAt                                                                                                                                          >  1:668777/1‑148 (MQ=255)
          ccGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATc                                                                                                                                         <  4:557057/149‑1 (MQ=255)
            ggCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCCATTGCgggtgatc                                                                                                                                       <  4:789115/149‑6 (MQ=255)
                aaGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGtt                                                                                                                                    >  2:96796/1‑148 (MQ=255)
                        cTGATTAGCGGCACTCAGGACGGCATCCGCGCCGGAGACGGACGCCCAATCGAGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGGAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCtt                                                                                                                           >  4:808865/1‑149 (MQ=255)
                          gATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCCATTGCgggtgat                                                                                                                                        >  1:266570/1‑130 (MQ=255)
                          gATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCCATTGCgggtgat                                                                                                                                        <  2:266570/134‑5 (MQ=255)
                           aTTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGt                                                                                                                        <  3:110380/149‑1 (MQ=255)
                              aTCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAg                                                                                                                     >  3:291581/1‑149 (MQ=255)
                                cGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGc                                                                                                                    <  1:96796/148‑1 (MQ=255)
                                  gCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGa                                                                                                                  <  1:846221/148‑1 (MQ=255)
                                  gCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGAt                                                                                                                 <  3:362354/149‑1 (MQ=255)
                                   cACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATc                                                                                                                >  3:373890/1‑149 (MQ=255)
                                      tCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGt                                                                                                             >  4:139570/1‑149 (MQ=255)
                                        aGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGc                                                                                                           <  4:774197/149‑1 (MQ=255)
                                          ggcggcATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCg                                                                                                          >  1:92021/1‑148 (MQ=255)
                                               cATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGgc                                                                                                    <  4:373890/149‑1 (MQ=255)
                                                 tCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGgcg                                                                                                   <  2:803750/148‑1 (MQ=255)
                                                  ccGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGgcgc                                                                                                  <  2:92021/148‑1 (MQ=255)
                                                     cgcCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCg                                                                                               >  1:328994/1‑148 (MQ=255)
                                                       ccGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAg                                                                                            <  3:119765/149‑1 (MQ=255)
                                                         ggAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAggg                                                                                          <  3:348060/149‑1 (MQ=255)
                                                          gagaCGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGgc                                                                                         <  3:538266/149‑1 (MQ=255)
                                                            gacggacgCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGgca                                                                                        <  1:176224/148‑1 (MQ=255)
                                                                     ccAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTAt                                                                              >  4:625410/1‑149 (MQ=255)
                                                                     ccAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTAt                                                                              >  3:131266/1‑149 (MQ=255)
                                                                        aTCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGt                                                                            <  1:738381/148‑1 (MQ=255)
                                                                          cgcgCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGTTCGCAGGGCACGCACGGTATGTTc                                                                          <  2:773649/148‑1 (MQ=255)
                                                                           gcgcTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGa                                                                        <  4:242908/149‑1 (MQ=255)
                                                                           gcgcTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGa                                                                        <  3:239178/149‑1 (MQ=255)
                                                                             gcTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGAtt                                                                      <  4:159191/149‑1 (MQ=255)
                                                                              cTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGgcgc                                                                                                  <  1:145354/120‑1 (MQ=255)
                                                                              cTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGgcgc                                                                                                  >  2:145354/1‑120 (MQ=255)
                                                                              cTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGAtt                                                                      >  1:666735/1‑148 (MQ=255)
                                                                                gggCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTccc                                                                   <  3:623478/149‑1 (MQ=255)
                                                                                 ggCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCt                                                                  <  3:347490/149‑1 (MQ=255)
                                                                                 ggCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCt                                                                  >  4:778369/1‑149 (MQ=255)
                                                                                    tACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGc                                                                <  1:376771/148‑1 (MQ=255)
                                                                                         cacCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTAc                                                           >  2:856985/1‑148 (MQ=255)
                                                                                              ggCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCaa                                                      <  2:668777/148‑1 (MQ=255)
                                                                                              ggCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCaa                                                      >  2:636964/1‑148 (MQ=255)
                                                                                                  ttGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGAt                                                                       >  2:873029/1‑127 (MQ=255)
                                                                                                  ttGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGAt                                                                       <  1:873029/127‑1 (MQ=255)
                                                                                                  ttGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAacgacg                                                 >  4:311812/1‑149 (MQ=255)
                                                                                                      gagaTCGGCATGCAGATGCAGGCGGCGGTGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTTGTCAGCGATCCGTGCGACGGCCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACgcgg                                              <  2:653893/148‑1 (MQ=255)
                                                                                                      gagaTCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACgcgg                                              >  1:659850/1‑148 (MQ=255)
                                                                                                        gaTCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCCTACCGCAACGACGCGGCGa                                           >  4:31922/1‑149 (MQ=255)
                                                                                                               atgcagatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGtt                                    >  3:660791/1‑149 (MQ=255)
                                                                                                                tgcagatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGtt                                    >  2:730108/1‑148 (MQ=255)
                                                                                                                 gcagatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTc                                  <  3:625410/149‑1 (MQ=255)
                                                                                                                  cagatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGTGATCGTGTTTc                                  >  1:367641/1‑148 (MQ=255)
                                                                                                                       gcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTcgccgc                             >  1:922440/1‑148 (MQ=255)
                                                                                                                        cagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCt                           <  3:511940/149‑1 (MQ=255)
                                                                                                                          ggcggcggcGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCgccgccgc                          >  1:275908/1‑146 (MQ=255)
                                                                                                                             ggcggcgAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATcc                      <  3:885732/149‑1 (MQ=255)
                                                                                                                                    aaGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCt               <  3:785571/149‑1 (MQ=255)
                                                                                                                                     aGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCt               >  1:910772/1‑148 (MQ=255)
                                                                                                                                      ggAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGc             >  3:441476/1‑149 (MQ=255)
                                                                                                                                             tcccGCCATTGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGAcgc       >  2:128509/2‑148 (MQ=255)
                                                                                                                                               ccGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCCCCCTGCCGACgcggc    >  3:946641/1‑149 (MQ=255)
                                                                                                                                                cGCCATTGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACgcggcg   >  3:930013/1‑149 (MQ=255)
                                                                                                                                                cGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACgcggc    >  1:363480/1‑148 (MQ=255)
                                                                                                                                                cGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACgcggc    >  2:820943/1‑148 (MQ=255)
                                                                                                                                                cGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACgcggc    >  1:23098/1‑148 (MQ=255)
                                                                                                                                                  cAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTTCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGcggcgg  >  1:102469/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGG  >  NC_000913/283369‑283662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑