Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,070,413 T→G 6.3% intergenic (‑12/+102) yihR ← / ← yihS putative sulphoquinovose mutarotase/sulphoquinovose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,070,4130TG 6.3% 378.8 / 11.0 119intergenic (‑12/+102)yihR/yihSputative sulphoquinovose mutarotase/sulphoquinovose isomerase
Reads supporting (aligned to +/- strand):  ref base T (62/48);  new base G (3/5);  total (66/53)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

Only 100 of 120 total aligned reads displayed.

CCTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAG  >  NC_000913/4070266‑4070558
                                                                                                                                                   |                                                                                                                                                 
ccTGAAAGGTTAATTCAGCCAGATCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGt                                                                                                                                                   <  1:855677/148‑1 (MQ=255)
 cTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACGAGGCACCTTAATTAACGACATACAGCCTCCCGTg                                                                                                                                                  >  1:618969/1‑148 (MQ=255)
 cTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGa                                                                                                                                                 <  3:888772/149‑1 (MQ=255)
 cTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATCAACGACATACAGCCTCCCGTg                                                                                                                                                  <  1:590395/148‑1 (MQ=255)
  tGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAg                                                                                                                                                >  4:798952/1‑149 (MQ=255)
  tGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAg                                                                                                                                                >  3:444887/1‑149 (MQ=255)
   gAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAg                                                                                                                                                <  1:267258/148‑1 (MQ=255)
   gAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAg                                                                                                                                                >  1:800074/1‑148 (MQ=255)
       ggTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCACGGGAGGCTgt                                                                                                                                           <  3:651716/149‑2 (MQ=255)
        gTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGAt                                                                                                                                          >  3:169929/1‑149 (MQ=255)
        gTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGAt                                                                                                                                          >  3:350592/1‑149 (MQ=255)
          tAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGt                                                                                                                                        >  3:559109/1‑149 (MQ=255)
          tAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGt                                                                                                                                        >  3:361945/1‑149 (MQ=255)
              tCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCaaa                                                                                                                                    <  3:895462/149‑1 (MQ=255)
              tCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCaa                                                                                                                                     <  1:255742/148‑1 (MQ=255)
                     gACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCaaa                                                                                                                              >  1:416444/1‑148 (MQ=255)
                      aCCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAg                                                                                                                                                >  4:561942/1‑129 (MQ=255)
                      aCCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGGCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAg                                                                                                                                                <  3:561942/129‑1 (MQ=255)
                            aCCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCa                                                                                                                       <  1:484843/148‑1 (MQ=255)
                                    tCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGT                                                                                                              >  4:700779‑M1/1‑149 (MQ=255)
                                       caATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAg                                                                                                                                                <  1:256606/112‑1 (MQ=255)
                                       caATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCC                                                                                                           >  4:657088‑M1/1‑149 (MQ=255)
                                       caATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCC                                                                                                            >  1:799603‑M1/1‑148 (MQ=255)
                                        aaTGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCC                                                                                                           <  2:903264‑M1/148‑1 (MQ=255)
                                         aTGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCC                                                                                                          >  2:829724‑M1/1‑148 (MQ=255)
                                             tCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGcc                                                                                                     >  4:504204/1‑149 (MQ=255)
                                                  tGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCtt                                                                                                 <  1:343715/148‑1 (MQ=255)
                                                  tGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCtt                                                                                                 >  1:270100/1‑148 (MQ=255)
                                                    aTAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTgg                                                                                              <  3:516324/149‑1 (MQ=255)
                                                     tAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTgg                                                                                              <  1:245816/148‑1 (MQ=255)
                                                      aaTCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTgg                                                                                              <  4:714342/147‑1 (MQ=255)
                                                      aaTCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTgg                                                                                              >  3:714342/1‑147 (MQ=255)
                                                         cccccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGgag                                                                                          >  1:210114/1‑148 (MQ=255)
                                                         cccccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGgag                                                                                          >  1:66622/1‑148 (MQ=255)
                                                          ccccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGgaga                                                                                         <  2:630948/148‑1 (MQ=255)
                                                          ccccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGagag                                                                                        <  4:679110/149‑1 (MQ=255)
                                                          ccccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGagag                                                                                        >  3:806715/1‑149 (MQ=255)
                                                           cccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGagag                                                                                        <  1:709284/148‑1 (MQ=255)
                                                           cccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGagag                                                                                        <  2:822532/148‑1 (MQ=255)
                                                           cccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAgg                                                                                       <  3:286731/149‑1 (MQ=255)
                                                           cccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAgg                                                                                       >  3:267027/1‑149 (MQ=255)
                                                           cccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAgg                                                                                       >  3:719316/1‑149 (MQ=255)
                                                            ccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACAGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAgg                                                                                       <  2:211615/148‑1 (MQ=255)
                                                              gcgGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGa                                                                                                                                                 >  3:714611/1‑88 (MQ=255)
                                                              gcgGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGa                                                                                                                                                 <  4:714611/88‑1 (MQ=255)
                                                                   aaGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAggg                                                                                <  1:646681/148‑1 (MQ=255)
                                                                    aGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGagag                                                                                        <  1:939032/139‑1 (MQ=255)
                                                                      tGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAgag                                                                            <  4:55362‑M2/149‑4 (MQ=255)
                                                                       gAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAgagg                                                                           <  4:383894‑M2/149‑5 (MQ=255)
                                                                             gTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAgg                                                                                 >  4:397974/1‑137 (MQ=255)
                                                                             gTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGaaaa                                                                     >  3:209453/1‑149 (MQ=255)
                                                                              tCTGTCCACTGAAATGCATCTTGGTGATTTGCATACCAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGGTGTCAAACCCAAAAACTGCACCGATCGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAgaggggagaac                                                                    >  4:462270‑M2/1‑138 (MQ=255)
                                                                                  tCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGc                                                                >  3:688234/1‑149 (MQ=255)
                                                                                   ccACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAggg                                                                                >  3:926562/1‑132 (MQ=255)
                                                                                   ccACTGCAATGAATATGGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCCCCCGTGGGCCTAATGTCAAACCCAAAACCTGCCCCGGACGGCCCCCTCGCCCCTTTGGGGGGAGGGTTAGGGAGAGGGGAAAACCCGc                                                                <  2:729620/148‑1 (MQ=255)
                                                                                    cACTGCAATGCGTATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCAc                                                              >  3:558645/1‑149 (MQ=255)
                                                                                     aCTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCAc                                                              >  1:799328/1‑148 (MQ=255)
                                                                                            tGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTAtttt                                                      >  4:754056/1‑149 (MQ=255)
                                                                                            tGCATATGGGTGATTTCCATACAGGGTACCTCAATTAACCACACGCAGCCCCCCGTGAGGCTGATGGAAACCAAAAAAACGGCGCAGGAGGGTCCCCGCGCTCTTTTGGGGGGGGGGTGCAGGAGGGGGAAAAACCCGCACCCTAtttt                                                      <  4:394699/149‑1 (MQ=255)
                                                                                              cATATTGGTGATTTGCAAACAAGCCACCTTAATTAACGCCATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAACCTGCCCCGGACGGCCCCCTCGCCCTTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCa                                                    <  3:95159/149‑1 (MQ=39)
                                                                                              aatatTGGTGTTTTCCAAACAAGCCCCTTTAATTAACGCCATACACCCTCCCGGGAGGCTGATGTCAAACCCAAAAACTGCCCCGGCCGGCCCCCTCGCCCTTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCa                                                    <  3:32145/148‑1 (MQ=255)
                                                                                               atatTGGTGTTTTGAAAACAAGGCACTTTAATAAAGAAAATACACCCTCCCGTGGGGCTGATGTCAAACCCAAAACCTGCACCGGACGGCCCCCTCGCCCCTTGGGGGAGGGGGTTGGGGAGAGGGGAAAACCCGCACCCTATTTTCAg                                                   <  3:383894/149‑1 (MQ=255)
                                                                                                 atTGGTGATTTGCATACAAGGCACCTTAATTAACGCCATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGAt                                                 <  4:719316/149‑1 (MQ=255)
                                                                                                  ttGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCtccctaaccctctccccaaaggggcgaggg                                                                          >  3:420677‑M1/1‑93 (MQ=255)
                                                                                                  ttGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCtccctaaccctctccccaaaggggcgaggg                                                                          <  4:420677‑M1/123‑31 (MQ=255)
                                                                                                   tGGTGATTTGCATGCAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCtccctaaccctctccccaaaggggcgagg                                                                           >  1:727451‑M1/1‑92 (MQ=255)
                                                                                                   tGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCtccctaaccctctccccaaaggggcgagg                                                                           <  2:727451‑M1/121‑30 (MQ=255)
                                                                                                   tGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATc                                                >  1:630743/1‑148 (MQ=255)
                                                                                                   tGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCa                                               <  4:267027/149‑1 (MQ=255)
                                                                                                    ggTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAg                                              >  4:867143/1‑149 (MQ=255)
                                                                                                     gTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGc                                                                                                      >  4:301971/1‑92 (MQ=255)
                                                                                                     gTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTa                                                                                   <  1:941350/111‑1 (MQ=255)
                                                                                                     gTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTa                                                                                   >  2:941350/1‑111 (MQ=255)
                                                                                                     gTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAg                                              >  2:132534/1‑148 (MQ=255)
                                                                                                              aTACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCGGATGTCTAACCCAAAAACTGCACCGGACGGCCCCCCCGCCCCTTTGGGGAGGGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGc                                     <  2:585918/148‑1 (MQ=255)
                                                                                                               tACAAGGCACCTTAATTAACGATATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCAt                                   >  3:378037/1‑149 (MQ=255)
                                                                                                                 cAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGT                                                                                                              <  2:447854‑M1/72‑1 (MQ=255)
                                                                                                                     gCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAACCTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAata                              <  2:813185/148‑1 (MQ=255)
                                                                                                                      cACCTTAATTAACGACATACAGCCTCCCGTGGGGCGGATGGCAAACCCAAAACCTGCCCCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAatat                             <  2:501550/148‑1 (MQ=39)
                                                                                                                        ctttaattaaCGACATACAGCCTCCCGGGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCca                          <  3:700779/147‑1 (MQ=255)
                                                                                                                          ttaattaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcag                         >  2:449090/1‑148 (MQ=255)
                                                                                                                          ttaattaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcag                         >  1:848246/1‑148 (MQ=255)
                                                                                                                               taaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGAc                    >  2:440993/1‑148 (MQ=255)
                                                                                                                               taaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGAc                    >  1:100504/1‑148 (MQ=255)
                                                                                                                               taaCGACATACAGCCTCCCGCGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGAc                    >  1:554365/1‑148 (MQ=255)
                                                                                                                                 aCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACGGCACCGGACGGTCCCCTCGCCCCTTGGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGAccc                  >  2:260049/1‑148 (MQ=255)
                                                                                                                                  cGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCg                 >  2:779972/1‑148 (MQ=255)
                                                                                                                                   gACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAGACTGCACCGGACGGTCCCCTCGCCCCTATGGGGAGAGGGTTAGGGAGAGGGGAAAACCCACACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCCGACCCGCt               >  3:593700/1‑149 (MQ=255)
                                                                                                                                   gACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCt               >  3:743168/1‑149 (MQ=255)
                                                                                                                                    ccttccAGCCCCCCGGGAGGTTGATGTCAAACCCAAAACCGGCACCGGAGGGACCCCTCGCCCCTTTGGGGAGGGGGTTAGGGGGAGGGAAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTg              <  4:743168/144‑1 (MQ=255)
                                                                                                                                      aTACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCa            >  4:170419/1‑149 (MQ=255)
                                                                                                                                       tACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGc                                     >  2:628258/1‑123 (MQ=255)
                                                                                                                                       tACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCaa           >  4:4036/1‑149 (MQ=255)
                                                                                                                                       atcAGCCTCACGGGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCaa           >  3:429682/3‑149 (MQ=255)
                                                                                                                                       atcAGCCTCACGGGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCaa           >  3:848092/3‑149 (MQ=255)
                                                                                                                                        aCAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCaa           >  1:492969/1‑148 (MQ=255)
                                                                                                                                        aCAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCaa           >  1:303284/1‑148 (MQ=255)
                                                                                                                                           gCCTCCCGTGAGGCTGATGCCAAACCCAAAACCGGCCCCGGCCGGTCCCCCCGCCCTTTTGGGGAGGGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCg        <  1:95245/148‑1 (MQ=255)
                                                                                                                                             cTCCCGTGAGGCTGATGTAAAACCCAAAACCTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTg     <  3:55362/149‑1 (MQ=255)
                                                                                                                                                ccGTGAGGCTGATGTCAAACCCAAAAACGGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACAGCTGGAg  <  4:688234/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CCTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAG  >  NC_000913/4070266‑4070558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: