Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,245,113 C→A 5.1% intergenic (‑38/+116) malF ← / ← malE maltose transporter subunit/maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,245,1130CA 5.1% 382.0 / 4.6 107intergenic (‑38/+116)malF/malEmaltose transporter subunit/maltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (60/41);  new base A (3/3);  total (63/44)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

GTGCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTCTTTCA  >  NC_000913/4244967‑4245260
                                                                                                                                                  |                                                                                                                                                   
gtgCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACg                                                                                                                                                    <  2:406894/148‑1 (MQ=255)
      aCATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCGTGAAAGCCTTAt                                                                                                                                              <  1:525181/148‑1 (MQ=255)
      aCATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCGTGAAAGCCTTATc                                                                                                                                             <  4:478624/149‑1 (MQ=255)
       cATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATc                                                                                                                                             >  1:237522/1‑148 (MQ=255)
       cATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATc                                                                                                                                             >  2:179958/1‑148 (MQ=255)
         ttAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCg                                                                                                                                           <  1:166964/148‑1 (MQ=255)
         ttAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCg                                                                                                                                           >  2:133586/1‑148 (MQ=255)
         ttAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGa                                                                                                                                          <  4:114266/149‑1 (MQ=255)
         ttAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGa                                                                                                                                          >  4:685007/1‑149 (MQ=255)
          tAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGa                                                                                                                                          >  1:784785/1‑148 (MQ=255)
           aaaaCTACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCACTTTGCCACCAATGTTTCATTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGaca                                                                                                                                        >  4:608149/1‑149 (MQ=255)
             aacaacAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGacaac                                                                                                                                      >  3:356099/1‑149 (MQ=255)
                   aaGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTg                                                                                                                                 <  1:566520/148‑1 (MQ=255)
                   aaGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATAGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGc                                                                                                                                >  3:695153/1‑149 (MQ=255)
                       tAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTg                                                                                                                             <  2:510035/148‑1 (MQ=255)
                       tAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTg                                                                                                                             <  1:300210/148‑1 (MQ=255)
                            caccaGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCg                                                                                                                        >  1:951765/1‑148 (MQ=255)
                            caccaGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCg                                                                                                                        >  1:769453/1‑148 (MQ=255)
                                   agGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGa                                                                                                                 <  1:725352/148‑1 (MQ=255)
                                     gCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGAcgc                                                                                                              >  3:514659/1‑149 (MQ=255)
                                       cGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGAcgcg                                                                                                             <  1:308959/148‑1 (MQ=255)
                                       cGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGAcgcg                                                                                                             <  1:616510/148‑1 (MQ=255)
                                         aGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTc                                                                                                           >  2:862664/1‑148 (MQ=255)
                                             gACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTAt                                                                                                       <  1:934942/148‑1 (MQ=255)
                                                cTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAg                                                                                                    >  1:771676/1‑148 (MQ=255)
                                                cTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAACGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAgg                                                                                                   >  3:695158/1‑149 (MQ=255)
                                                 tAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAgg                                                                                                   >  2:686780/1‑148 (MQ=255)
                                                 tAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGc                                                                                                  <  3:753566/149‑1 (MQ=255)
                                                  aGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGc                                                                                                  >  1:814429/1‑148 (MQ=255)
                                                   gCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACaacaac                                                                                                                                   <  2:550090/114‑1 (MQ=255)
                                                   gCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACaacaac                                                                                                                                   >  1:550090/1‑114 (MQ=255)
                                                           cATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACg                                                                                         <  2:686268/148‑1 (MQ=255)
                                                           cATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGt                                                                                        >  3:741191/1‑149 (MQ=255)
                                                                cAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCgg                                                                                   >  4:133487/1‑149 (MQ=255)
                                                                  gcgcGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGtt                                                                                 <  4:741191/149‑1 (MQ=255)
                                                                    gcgTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGtttt                                                                               <  3:119914/149‑1 (MQ=255)
                                                                    gcgTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGttt                                                                                <  2:717397/148‑1 (MQ=255)
                                                                     cgTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGtttt                                                                               <  1:252941/148‑1 (MQ=255)
                                                                      gTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTGTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGt                                                                             >  3:544827/1‑149 (MQ=255)
                                                                      gTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGt                                                                             >  4:199012/1‑149 (MQ=255)
                                                                      gTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGt                                                                             <  4:36290/149‑1 (MQ=255)
                                                                         gCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAgg                                                                          >  3:295814/1‑149 (MQ=255)
                                                                          cTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAgg                                                                          >  1:715504/1‑148 (MQ=255)
                                                                           tttGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGcc                                                                        >  4:641408/1‑149 (MQ=255)
                                                                           tttGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGcc                                                                        >  4:164991/1‑149 (MQ=255)
                                                                               ccaccaATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGAc                                                                    >  3:375354/1‑149 (MQ=255)
                                                                               ccaccaATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCAGa                                                                     >  1:739764/1‑148 (MQ=255)
                                                                                  ccaATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACaa                                                                  <  1:686780/148‑1 (MQ=255)
                                                                                  ccaATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACaa                                                                  >  1:687191/1‑148 (MQ=255)
                                                                                   caATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAgg                                                                >  3:353859/1‑149 (MQ=255)
                                                                                   caATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCGTGAAAGCCTTATCCgtc                                                                                                                                         >  3:746697/1‑74 (MQ=25)
                                                                                   caATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCGTGAAAGCCTTATCCgtc                                                                                                                                         <  4:746697/76‑3 (MQ=25)
                                                                                    aaTGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAgg                                                                <  1:50546/148‑1 (MQ=255)
                                                                                     aTGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGt                                                                             <  2:795472/134‑1 (MQ=255)
                                                                                     aTGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGt                                                                             >  1:795472/1‑134 (MQ=255)
                                                                                       gTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGtt                                                            <  3:641408/149‑1 (MQ=255)
                                                                                       gTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGtt                                                            >  3:780622/1‑149 (MQ=255)
                                                                                       gTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGt                                                             <  2:867038/148‑1 (MQ=255)
                                                                                          tCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCa                                                          <  1:133586/148‑1 (MQ=255)
                                                                                            tttttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCAcgc                                                       >  4:57650/1‑149 (MQ=255)
                                                                                            tttttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCAcgc                                                       <  4:514659/149‑1 (MQ=255)
                                                                                            tttttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCAcgc                                                       >  3:514683/1‑149 (MQ=255)
                                                                                             ttttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCAcgc                                                       >  1:865955/1‑148 (MQ=255)
                                                                                             ttttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCAcgc                                                       >  2:792877/1‑148 (MQ=255)
                                                                                             ttttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCAcgc                                                       >  2:779661/1‑148 (MQ=255)
                                                                                                 aaTGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCAt                                                  >  4:217141/1‑149 (MQ=255)
                                                                                                  aTGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTGAAcgccttgt                                                  <  2:249806/148‑5 (MQ=255)
                                                                                                   tGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATc                                                 <  2:951765/148‑1 (MQ=255)
                                                                                                    gACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATcc                                                >  2:521947/1‑148 (MQ=255)
                                                                                                     aCATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCg                                               >  1:889889/1‑148 (MQ=255)
                                                                                                       aTCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCa                                            >  3:645293/1‑149 (MQ=255)
                                                                                                           aTGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGAGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTc                                        >  4:68033/1‑149 (MQ=255)
                                                                                                             ggggTTCTTCTTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTcac                                      >  3:61652/1‑149 (MQ=255)
                                                                                                              gggTTCTTCCTCATTCTAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTcaca                                     <  3:608149/149‑1 (MQ=255)
                                                                                                                gTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGc                                   <  3:945693/149‑1 (MQ=255)
                                                                                                                   cttcCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCAt                                 <  1:782145/148‑1 (MQ=255)
                                                                                                                       cTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACtt                            >  4:239651/1‑149 (MQ=255)
                                                                                                                        tCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACtt                            >  1:115572/1‑148 (MQ=255)
                                                                                                                         cATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTgg                          <  4:356099/149‑1 (MQ=255)
                                                                                                                         cATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTgg                          >  3:659441/1‑149 (MQ=255)
                                                                                                                          aTTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACCAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGt                         <  3:585014/149‑1 (MQ=255)
                                                                                                                          aTTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTgg                          <  2:282137/148‑1 (MQ=255)
                                                                                                                            tCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTg                        <  1:969521/148‑1 (MQ=255)
                                                                                                                            tCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGa                       <  3:887547/149‑1 (MQ=255)
                                                                                                                               aGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATa                     <  2:115572/148‑1 (MQ=255)
                                                                                                                               aGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATa                     <  1:521947/148‑1 (MQ=255)
                                                                                                                               aGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATAc                    >  3:319424/1‑149 (MQ=255)
                                                                                                                                ggACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATAc                    >  2:932905/1‑148 (MQ=255)
                                                                                                                                ggACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACg                   >  4:374768/1‑149 (MQ=255)
                                                                                                                                 gtcGGATAAGGCGTGAAAGCCTTATCCGTCAACAACTGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGa                  >  3:49212/3‑149 (MQ=37)
                                                                                                                                  tcGGATAAGGCGTGAAAGCATTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGa                  >  2:700209/2‑148 (MQ=37)
                                                                                                                                       tAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTgc            >  4:776748/1‑149 (MQ=255)
                                                                                                                                       tAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTg             >  1:852315/1‑148 (MQ=255)
                                                                                                                                          ggCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCCTTACTTGGTGATACGAGTCTgcgc          >  2:908540/1‑148 (MQ=255)
                                                                                                                                            cTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCa                                                                                                     >  1:605849/1‑55 (MQ=255)
                                                                                                                                            cTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCa                                                                                                     <  2:605849/55‑1 (MQ=255)
                                                                                                                                            cTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGt        >  1:844466/1‑148 (MQ=255)
                                                                                                                                             tttCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTCt      >  4:875440/1‑149 (MQ=255)
                                                                                                                                              ttCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTCt      >  1:259921/1‑148 (MQ=255)
                                                                                                                                                 aCGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTCTTTCa  <  3:471920/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GTGCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTCTTTCA  >  NC_000913/4244967‑4245260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: