Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,3801.C99.0% 306.5 / ‑3.4 100intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base C (47/52);  total (48/52)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01
*NC_0009134,296,3802.G98.0% 297.6 / NA 100intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  major base G (47/50);  minor base C (0/2);  total (48/52)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCG  >  NC_000913/4296170‑4296646
                                                                                                                                                                                                                   ||                                                                                                                                                                                                                                                                              
cAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGt                                                                                                                                                                                                              >  2:693023/1‑276 (MQ=2)
      cttaTGCGATGCTTGCCGTGTCTTATCAGGCCTACGCCAGCCGGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTCGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTa                                                                                                                                                                                                        <  2:443237/273‑1 (MQ=11)
       tgctGCGACGCTTGCGCCGTCTTATCAGGCCTACGCCAGACGGCGCAATAGCCTGATTTGGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTa                                                                                                                                                                                                        <  1:248770/272‑1 (MQ=11)
        gATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTaa                                                                                                                                                                                                       >  2:457953/1‑275 (MQ=9)
         aTGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAAt                                                                                                                                                                                                      >  1:535487/1‑275 (MQ=9)
                 cttgccccttCTTATCAGGCGTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCaaa                                                                                                                                                                                              <  1:815370/266‑1 (MQ=255)
                    gccgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTGACGC‑C‑GCATCCGACATCAATGCCAGATGCGACGCTTGCCGCGTCTTACCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAAct                                                                                                                                                                                            >  1:484202/3‑274 (MQ=255)
                          tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCCTCCGACATCCATGCCTGATTCCTCGCTTGCCGCGTCTTATCCGGCCTATCTTAA‑CCCTTAGTTAATTTTTCCACCTCTTcaca                                                                                                                                                                                     >  4:590059/1‑275 (MQ=11)
                            ttATCAGGCCTACGCCAGACAGCGCCAGAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                                                                   <  2:346774/275‑1 (MQ=255)
                            ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACAccc                                                                                                                                                                                  <  2:588727/276‑1 (MQ=255)
                             tctCAGACCTACGCCAGGAACCGAAAGAGCCTGATTTAGCGTGATTTTGTCGGTCGGATAAGGCGTTTAGGCCGCGTCCGACATCAACGCCTGATGGGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                                                                   <  3:390649/272‑1 (MQ=14)
                             tATCAGGCCTACGCCAGAAAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACAccc                                                                                                                                                                                  <  1:825744/275‑1 (MQ=255)
                             gaTCAAGCCTACGACAGCGAGGACAATAGCCTGATTTAGCGTGAGTTTGCAGGGCGGATAAGGCGTTTATGCCTCCTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTAAGCCAGACATCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACAccc                                                                                                                                                                                  <  1:863929/274‑1 (MQ=14)
                                    ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCGTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTCAATTTTTCACACTCTTCACACCCGGTATcc                                                                                                                                                                           >  3:861916/1‑274 (MQ=255)
                                    ccTAAGCCAGGCAGGGGAATAGCCTGATTTGGCGTGATTTTGTAGGGCGGAGAAGGCGTTTATTCCGCATCCGACATCAAAGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCa                                                                                                                                                                           <  3:125307/275‑1 (MQ=14)
                                      gaCGCCAGACAGCGGAATGGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCAGCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                                                                         <  2:29788/274‑1 (MQ=255)
                                       aCGCCGGACATCGCAAGAGCTGGATATAGCATTATTTCATTGGGGGGGTAAGGCGTTTATGACGCATGCGACATAGAGGCTTGATGCGACGCTTAAAGCTTGTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                                                                         <  3:530596/274‑1 (MQ=14)
                                         gCCAGACCGCACAATTGCCTGATTTAGCGTGGGTGTTTAGGTCGGATAAGGCGTTTATGCCGCATCCGCCTTCAACCCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCAGATAAGGCGTTTACGC‑C‑GCAACCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                                                                         <  1:440612/272‑1 (MQ=14)
                                              aCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTcctt                                                                                                                                                                >  2:294268/1‑274 (MQ=255)
                                                agggaaaTAGCCTGATTTAGCGTGATTGTGTATGTGGGATAAGGCGGTTATGCCGCATCCGACATAAACGCCTGAGGCGACGCGTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa                                                                                                                                                               <  2:133887/270‑1 (MQ=17)
                                                 gcgcAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa                                                                                                                                                               <  2:38701/274‑1 (MQ=255)
                                                 gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTAAACGTGATTTTGTCGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACACCAATGCCTGATTCTACGCTTGCCGCGTCTCATCCGCCCTACCTTAA‑CCTTTCGTTATTTTTTCAAACTCTTCACCACAGCTCTCAAATCCttaccgca                                                                                                                                                              >  4:480227/1‑269 (MQ=11)
                                                  ggcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCAGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTGCACACCCGGTATCAAACCCTTCCATa                                                                                                                                                               <  1:533166/272‑1 (MQ=255)
                                                  cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTTAATTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACCCTCTGTATCCAACCCTTcccatca                                                                                                                                                             >  3:523638/1‑270 (MQ=14)
                                                  cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTTCCGCGTCTTATCAGGCCTATCTTCA‑CCGTTTGTTAATTTTTCACACTCTGCACACCCCGTATCAAACCCTTCCAttaa                                                                                                                                                             >  3:430640/1‑272 (MQ=14)
                                                  cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCTCTTAA‑CCGTTGGTTAATTTTTTAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                                              >  1:756226/1‑274 (MQ=255)
                                                  cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCCGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATACACCCCTTcagtccag                                                                                                                                                            >  2:399247/1‑269 (MQ=14)
                                                  cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATACGTCATCAATGCCTGATGCGACGCTTGCCGCGTCTTATAAGGCCTATCTTAA‑CCGTTGTTTAATTTTTCAAACTCTTCACACCCGGTATACAACCCTCCCTTAc                                                                                                                                                              >  1:618021/1‑274 (MQ=14)
                                                    cAATAGCCGGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                                                                                                                                          <  2:786039/276‑1 (MQ=255)
                                                     acaaGCCTGATTTAGCGTGAGTTTGTAGGGCGGATAAGGCGTTTATGCCGCATCCGACATCAAAGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTGGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                                                                                                                                          <  1:201135/272‑1 (MQ=255)
                                                     aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATATCCTGCTTTCGCTTGCTTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATAAATCACTGATTCGACCCTTGCCGATTCTTATCAGGCCTATCTTAC‑CCGTTGTTTCCTTTTTCCACCTCTTAACACTCTGTATCACACACTTCCATACa                                                                                                                                                             >  4:618378/1‑272 (MQ=11)
                                                       tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGGCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTGTTCACACCCGGTATGAAACCCCTCCATAcctgcct                                                                                                                                                        >  2:425098/1‑269 (MQ=14)
                                                            tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACACCTCAGATTc                                                                                                                                                   >  2:601968/1‑275 (MQ=255)
                                                             gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTAAGCGTGATTTTGTAGGTCGGATAAGTCGTTTACGC‑C‑GCATCCGACCTCAATCCCTGAGTCGACGCTTGCCGCGTCTTATCAGGCCTCTCTTAA‑CCTTTGGTTAATTTTTCACACCCTTCACACCCGTTATCAAACCCTTCCaatactccccggat                                                                                                                                                    >  3:282198/1‑260 (MQ=14)
                                                                    cGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCCTCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATAAAACCCTTCCATAAAGCTCATGTGCAACCAGTga                                                                                                                                          >  2:574007/1‑275 (MQ=255)
                                                                    cGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAc                                                                                                                                                                        >  1:37811/1‑246 (MQ=255)
                                                                        aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCtt                                                                                                                                                                    <  3:37811/246‑1 (MQ=255)
                                                                        aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATCCACATCAGGTTCAACCATTG‑ccac                                                                                                                                      >  1:517946/1‑272 (MQ=255)
                                                                        aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCCTTG‑CAgc                                                                                                                                      >  1:517568/1‑275 (MQ=255)
                                                                             ttAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCt                                                                                                                                  <  1:526270/273‑1 (MQ=255)
                                                                                   cGGATAAGGCGTTTATGCCGCATCCGACATCAACGTCTGATGCGACCCTTAACGCGTCTTATCAGCCCTACGCCACACAGCGTAATAGCCCGATCGATTGTGATTTCGCAGGTCGCATAAGGCGTTTACGC‑C‑GCATCCGACATCACTGACTGATGCGACGCTTGCCGCGACTTATGTTGACTATCTTCC‑TCGTTTGTTAATTTTTCCTACTCTTCACACCTGTTACCAATCTCATCCATATAGCCCAGGTTCACCCAGTT‑CAgtccctgtagcctg                                                                                                                          >  4:175654/1‑263 (MQ=2)
                                                                                      aTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                                                                         <  2:410435/227‑1 (MQ=255)
                                                                                        aaGGCGTTGATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGttctt                                                                                                                     <  2:590059/276‑1 (MQ=255)
                                                                                         aGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGttctt                                                                                                                     <  1:861917/275‑1 (MQ=255)
                                                                                          gacttttATGCCCCATACCACACCAACGCCTGTGGCGACGCTTAACGCGTCTTATCAGGGCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTtcttct                                                                                                                   <  4:457953/272‑1 (MQ=21)
                                                                                               ttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGAt                                                                                                               >  2:318364/1‑275 (MQ=255)
                                                                                                tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCATACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGCTGGTTAATCTTTCCACCTCTTCACACCCGTGATCAAACCCTTCACTACATATTAGGTTCAACCAGTG‑CAGCCCCTTTTTCTTGTTCTTCTCGATaa                                                                                                             >  4:42924/1‑276 (MQ=17)
                                                                                                 aTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATaaa                                                                                                            <  2:638365/276‑1 (MQ=255)
                                                                                                     cGCAGCCGCCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAAc                                                                                                          <  1:476515/274‑1 (MQ=255)
                                                                                                      gCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCACCCAGTG‑CAGCGCCTTTTGCTTTTTCTTCCCCATAAAACctt                                                                                                       >  2:360371/1‑276 (MQ=255)
                                                                                                       cATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTCATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGt                                                                                                                                            >  4:516726/1‑239 (MQ=255)
                                                                                                          ccGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑Ca                                                                                                                                        <  2:516726/239‑1 (MQ=255)
                                                                                                           cGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCa                                                                                                  <  2:616744/276‑1 (MQ=255)
                                                                                                            gACATCAACGCCTGATCCGACGCTTAACGCGTCTTATCAGGACTACGCCAGACATCGCAATAGGCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCAc                                                                                                 <  2:226387/276‑1 (MQ=255)
                                                                                                            caCATCAACCCCTGATGCCACGCTTTACGCGGCTTATCAGGCCTACGCCAGACAGCGGAGTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCa                                                                                                  <  3:535486/274‑1 (MQ=25)
                                                                                                                    cGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCGATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCNTTTACGC‑C‑GCATCCGACAGCAATGCCTGATGCGCCTCTTGCTGCGTCTTACCAGCCCCATCCTAA‑CCGTTGGTTAATTTTTCAAACTCTGCACACCAGGTATCAAACTCTTCCATACAGCCCAGGTTC‑CCCAATGACAGCGATGTTTGTTTATCATTCTCGGTAAAACTCTCTCCACCGTTTaa                                                                                          >  4:428494/1‑275 (MQ=17)
                                                                                                                    cGCCTGAGGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAg                                                                                         <  2:347988/276‑1 (MQ=255)
                                                                                                                     gCCTTATACGACGCTTAAGGTGTATTATCAGGCCTACGACAGACAGCGCAATAGACTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATACGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCTGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAg                                                                                         <  2:591439/275‑1 (MQ=25)
                                                                                                                              gACGCTTAACGCGTCTTATCAGGCATACGCAAGACAGCGCATTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTc                                                                               <  2:469683/276‑1 (MQ=255)
                                                                                                                                 gCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTccc                                                                             <  1:405906/275‑1 (MQ=255)
                                                                                                                                  cTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAAAGCTCAGGTTCAACCAGTG‑CAGCCCCTTTTGCTTGTTCTTCTCGATCAAACCTTCTCCACCGTTTAAGAACATCATTcact                                                                            >  1:59093/1‑272 (MQ=255)
                                                                                                                                   taaaccctccTTATCA‑GCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGcc                                                                          <  1:714229/266‑1 (MQ=255)
                                                                                                                                    tagagcgGCTTATCAGGACTACGCCGGACAGCGAAATAGCCTGATTTGGCGTGGTTTTGGCGGGCGGAGAAGTCGTTTACGC‑C‑GCAGCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGcc                                                                          <  4:507617/271‑1 (MQ=25)
                                                                                                                                     aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGcc                                                                          <  1:648534/274‑1 (MQ=255)
                                                                                                                                       cgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAg                                                                       >  1:297414/1‑275 (MQ=255)
                                                                                                                                         ggttttATAAGGCATACGCCAGACAGCGCAATCGCCTGATTTGGCGTGATTTTGTAGGTCGGATAAGCCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTa                                                                     <  3:425306/271‑1 (MQ=37)
                                                                                                                                           tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACa                                                                                                                                                             >  2:651720/1‑186 (MQ=255)
                                                                                                                                           tCTTATCAGGCCTACACCAGCAAGGGGCATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCAGACCTCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCTCTTAA‑CCGTTGGTTAATTTTTCCAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTc                                                                  <  4:259072/276‑1 (MQ=37)
                                                                                                                                              tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                                                                                                                                          <  4:651720/186‑1 (MQ=255)
                                                                                                                                                    gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCTACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAAc                                                          >  1:105608/1‑275 (MQ=255)
                                                                                                                                                    gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑CGCAGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCACACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGCACATCATTCCCGCCCAGTACTCGGAATacc                                                          >  1:580440/1‑273 (MQ=255)
                                                                                                                                                    gCCTACGCCAGACAGCGCAATAGCCTGATTTAGAGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTT‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCGCCTTCACCGTTTAAGAACATCATTCCCGCCCTGTACACGGAaaaat                                                          >  3:551456/1‑274 (MQ=37)
                                                                                                                                                     ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGCACCACATTCACGCCCAGTAATAGGAATCAcc                                                         >  4:375876/1‑275 (MQ=37)
                                                                                                                                                         cGCCAGACAGCGCAATAGCCTGATTTAGCGTGGTTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAACCCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGtt                                                     <  2:433081/275‑1 (MQ=255)
                                                                                                                                                         cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCACACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGCTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGCTAAAACCTTCTTCACCGTTTAAGAACATCTTTCCCGCCCAGCACTCGGAATCACCGGtgc                                                    >  4:133427/1‑274 (MQ=37)
                                                                                                                                                         cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGt                                                                      >  2:351623/1‑258 (MQ=255)
                                                                                                                                                         cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGtt                                                     >  3:205624/1‑275 (MQ=255)
                                                                                                                                                         cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCCCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCATTACTCGGAATAACAGGtcc                                                    >  4:181064/1‑274 (MQ=255)
                                                                                                                                                            cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACt                                                                   <  4:351623/258‑1 (MQ=255)
                                                                                                                                                             ctaCAGCGCAAGATCATGATTTAGCGTGATTTTGTTGGTCGGAGAAGGCGTTTACGC‑C‑CCATCCTACATCAATGCCTGATGCGACGCTTACCGCTTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTN‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGa                                                <  4:432090/274‑1 (MQ=255)
                                                                                                                                                              tgcAGCGCAATTGCCTAATTTAGCGTTATTTTGTAGTTCGGCTAAGGCGTTTACCC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGa                                                <  3:24689/273‑1 (MQ=255)
                                                                                                                                                              gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGa                                                <  1:252198/275‑1 (MQ=255)
                                                                                                                                                              gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGa                                                <  1:677362/275‑1 (MQ=255)
                                                                                                                                                              gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGa                                                <  3:297413/275‑1 (MQ=255)
                                                                                                                                                              caccgcgcAATAGCCGTATTTAACCTGATTTTTTAGGTCGTATAAGGCGTTTACCC‑C‑GCACCCGACATCAATGCCAGCGTGGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGa                                                <  3:484201/271‑1 (MQ=255)
                                                                                                                                                               aCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGaaa                                              >  2:760006/1‑276 (MQ=255)
                                                                                                                                                                   cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCCGTACTCGGAATACCCGGTTCGGGAAATTGc                                          >  2:735145/1‑276 (MQ=255)
                                                                                                                                                                   cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGc                                          >  2:498033/1‑276 (MQ=255)
                                                                                                                                                                             tGATTTAGCGTTATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCCTGCCGCTTCTTCTCCGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTTACACCCGGTATCAAACCCTTCCATCCAGCTCGGGTTCAACCCGTT‑CAACGGCTTTTGCTTTTTCTTCTCGATCAAACCTTCTTCACCTCTTACTAACATCTTTCCCGTCCGACTCTCGGAATAACCTGGTCGTCCAATTGCAGAGCtacct                                >  4:270065/1‑272 (MQ=21)
                                                                                                                                                                                tttgcgttGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTg                             <  2:513960/269‑1 (MQ=255)
                                                                                                                                                                                         aTTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTc                                                                                                                                                                   >  1:654184/1‑134 (MQ=255)
                                                                                                                                                                                          ttttGTATGTCGGATAAGGCGTTTACGC‑C‑GCAGCCGACATCAATGCCGTGTGCGACGCGTGCCGCGTCTTATCAGGCCTATCGTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAgg                   <  4:25398/276‑1 (MQ=255)
                                                                                                                                                                                             tGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa                                                                                                                                                               <  3:654183/134‑1 (MQ=255)
                                                                                                                                                                                               tAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTT‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTACCGCCCAATACTCGGAATAACCGGCTCGGGAAATTGCCGAGCTTCGTTTGAAATCCCAGGttc                >  3:750220/1‑274 (MQ=38)
                                                                                                                                                                                               tAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑TAGCGCCTCTTGCTTGTTCTTCTTGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGTATAACCGGTTCTGGCAATTTCACAGTTCCCTTTGCAATACAAGGtttc               >  2:376681/1‑273 (MQ=38)
                                                                                                                                                                                                cggTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATAAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGcc              <  3:239798/274‑1 (MQ=255)
                                                                                                                                                                                                    cGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGTCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAATT‑CATCGCCTGTTGCTTGTTCTTCTCGATACAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACCCGGAATCACCGGTTCGGCAAATTGAAGAGCTGCGTTTTAATTACAAGGTTGCCTTGt          >  3:181363/1‑275 (MQ=38)
                                                                                                                                                                                                    cGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTc         >  2:435721/1‑276 (MQ=255)
                                                                                                                                                                                                       aTAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACAAGTG‑CAGCGCCTTTTGCTTGTTCTTCTAGATAAAACCTTCTTCACCGTTTAAGAACCTCATTACCGCCCATTACTCGGAATACCAGGCTTGGGAACTTGCCGCGCTGCGTGTGAAATACCAGGTTCCTTTGTcg        >  4:677473/1‑273 (MQ=38)
                                                                                                                                                                                                           ggCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCAtcgtcg  <  2:133427/276‑1 (MQ=255)
                                                                                                                                                                                                                   ||                                                                                                                                                                                                                                                                              
CAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTG‑CAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCG  >  NC_000913/4296170‑4296646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑