Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,113,548 T→C 7.8% intergenic (+142/‑31) opgH → / → yceK OPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist/outer membrane integrity lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,113,5480TC 7.8% 427.5 / 20.4 141intergenic (+142/‑31)opgH/yceKOPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist/outer membrane integrity lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (59/70);  new base C (7/4);  total (66/75)
Fisher's exact test for biased strand distribution p-value = 3.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01

Only 100 of 143 total aligned reads displayed.

GCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATCCCGGTGTGCAATGG  >  NC_000913/1113400‑1113695
                                                                                                                                                    |                                                                                                                                                   
gCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTAt                                                                                                                                                     <  1:72777/149‑1 (MQ=255)
  aaTAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGc                                                                                                                                                   >  1:855145/1‑149 (MQ=255)
  aaTAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGc                                                                                                                                                   <  1:941045/149‑1 (MQ=255)
  aaTAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGc                                                                                                                                                   >  2:101966/1‑149 (MQ=255)
  aaTAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGc                                                                                                                                                   >  1:378196/1‑149 (MQ=255)
    tAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCg                                                                                                                                                 >  4:311862/1‑149 (MQ=255)
       aaaCGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAGTAAAAATACCGGCATAACGCCGGTa                                                                                                                                              >  3:688241/1‑149 (MQ=255)
        aaCGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCTGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAt                                                                                                                                             <  3:147100/149‑1 (MQ=255)
         aCGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAtt                                                                                                                                            <  1:1029111/149‑1 (MQ=255)
           gtaatcgCCTGATGCGCTACGCTTATTAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCTAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAtttt                                                                                                                                          >  4:668552/7‑149 (MQ=255)
              gTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAttttttt                                                                                                                                       >  4:1002563/1‑149 (MQ=255)
               ttGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCTGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAtttttatt                                                                                                                                      <  4:688241/149‑4 (MQ=37)
               ttGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAtttttatt                                                                                                                                      <  1:245749/149‑4 (MQ=255)
                tGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTAc                                                                                                                                     <  4:214891/149‑1 (MQ=255)
                   cTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAttt                                                                                                                                           >  2:185493/1‑140 (MQ=255)
                    tGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGaaa                                                                                                                                 <  2:1022306/149‑1 (MQ=255)
                       tGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAagag                                                                                                                              <  3:482738/149‑1 (MQ=255)
                            tACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATc                                                                                                                         >  4:934763/1‑149 (MQ=255)
                             aCGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCa                                                                                                                        >  2:1009035/1‑149 (MQ=255)
                                cTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAAt                                                                                                                     >  2:499463/1‑149 (MQ=255)
                                 ttATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATg                                                                                                                    >  3:601419/1‑149 (MQ=255)
                                  tATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGc                                                                                                                   <  2:902565/149‑1 (MQ=255)
                                  tATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGc                                                                                                                   <  3:934763/149‑1 (MQ=255)
                                  tATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGc                                                                                                                   <  3:1036666/149‑1 (MQ=255)
                                   aTCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCg                                                                                                                  >  1:716191/1‑149 (MQ=255)
                                    tCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGt                                                                                                                 <  1:445429/149‑1 (MQ=255)
                                     cAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGtt                                                                                                                <  1:411664/149‑1 (MQ=255)
                                        gCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTaa                                                                                                             >  3:296042/1‑149 (MQ=255)
                                            aCATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATtgt                                                                                                         <  1:166114/149‑1 (MQ=255)
                                             cATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATtgtg                                                                                                        <  2:841410/149‑1 (MQ=255)
                                             cATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATtgtg                                                                                                        <  1:282361/149‑1 (MQ=255)
                                             cATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATtgtg                                                                                                        <  1:651634/149‑1 (MQ=255)
                                               tCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTgtggt                                                                                                      <  3:1002563/149‑1 (MQ=255)
                                                 gTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGa                                                                                                    >  3:753530/1‑149 (MQ=255)
                                                 gTTCCTGCAATTTATTCATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGa                                                                                                    <  3:668552/149‑1 (MQ=255)
                                                  ttCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAg                                                                                                   >  1:537068/1‑149 (MQ=255)
                                                   tCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGc                                                                                                  <  2:887033/149‑1 (MQ=255)
                                                      tGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAGTTGTGGTGAGc                                                                                                  <  3:614566/146‑1 (MQ=255)
                                                      tGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGcatg                                                                                               >  3:93016/1‑148 (MQ=255)
                                                      tGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGc                                                                                                  >  4:614566/1‑146 (MQ=255)
                                                      tGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATAAAATGCGTTTAATTGTGGTGAGcatc                                                                                               >  2:650888/1‑149 (MQ=255)
                                                        cAATTTATTGATTTTGCAAGTCTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGcatcat                                                                                             <  1:153974/149‑1 (MQ=255)
                                                         aaTTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATg                                                                                            <  3:435044/149‑1 (MQ=255)
                                                         aaTTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATg                                                                                            <  4:296042/149‑1 (MQ=255)
                                                              aTTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGAc                                                                                       <  4:217293/149‑1 (MQ=255)
                                                               ttGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGAcc                                                                                      >  2:956386/1‑149 (MQ=255)
                                                                   ttttGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGc                                                                                  <  2:451029/149‑1 (MQ=255)
                                                                    tttGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCt                                                                                 <  4:223259/149‑1 (MQ=255)
                                                                      tGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGa                                                                               <  4:595371/149‑1 (MQ=255)
                                                                        cAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGt                                                                             <  4:412500/149‑1 (MQ=255)
                                                                        cAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGt                                                                             >  3:573325/1‑149 (MQ=255)
                                                                        cAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGt                                                                             <  1:424666/149‑1 (MQ=255)
                                                                        cAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGt                                                                             >  1:962628/1‑149 (MQ=255)
                                                                        cAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGt                                                                             <  4:753530/149‑1 (MQ=255)
                                                                         aaGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTg                                                                            >  4:589799/1‑149 (MQ=255)
                                                                         aaGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATCCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGt                                                                                          >  4:1002436/1‑135 (MQ=255)
                                                                          aGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTgg                                                                           <  2:257586/149‑1 (MQ=255)
                                                                           gACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAAt                                                                                                                     >  1:842679/1‑106 (MQ=255)
                                                                            aCTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCt                                                                         <  3:780141/149‑1 (MQ=255)
                                                                              tttGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCtgt                                                                       <  2:336247/149‑1 (MQ=255)
                                                                              tttGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCtgt                                                                       <  1:944106/149‑1 (MQ=255)
                                                                                tgtgGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTgg                                                                     <  3:311862/149‑1 (MQ=255)
                                                                                 gtgGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgc                                                                    >  4:114684/1‑149 (MQ=255)
                                                                                   gggTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgcag                                                                  >  2:630282/1‑149 (MQ=255)
                                                                                      tCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCat                                                               <  2:516446/149‑1 (MQ=255)
                                                                                         gATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCattat                                                            >  3:476249/1‑149 (MQ=255)
                                                                                              ggCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTc                                                       <  1:785169/149‑1 (MQ=255)
                                                                                              ggCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTc                                                       >  2:615888/1‑149 (MQ=255)
                                                                                                 gTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGCGGCTGTGGCAGCATTATTAGTCGCa                                                    >  1:650103/1‑149 (MQ=255)
                                                                                                    cACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACta                                                 >  4:383748/1‑149 (MQ=255)
                                                                                                       gccgcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATAc                                              <  2:608212/149‑1 (MQ=255)
                                                                                                          gcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCgg                                           <  2:336242/149‑1 (MQ=255)
                                                                                                           cATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggg                                          <  3:911617/149‑1 (MQ=255)
                                                                                                                ggCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcagg                                     <  1:650888/149‑1 (MQ=255)
                                                                                                                 gCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcaggg                                    >  2:674031/1‑149 (MQ=255)
                                                                                                                 gCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcaggg                                    <  4:601419/149‑1 (MQ=255)
                                                                                                                   aaaaaCAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGCGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGTGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcaggggc                                  >  1:107167/1‑149 (MQ=255)
                                                                                                                     aaaCAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTGTCAAATGCGTTTAATTGTGGTGAGCAGCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCAt                                <  2:558734/149‑1 (MQ=255)
                                                                                                                     aaaCAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAg                                                         <  2:960479/124‑1 (MQ=255)
                                                                                                                     aaaCAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAg                                                         >  1:960479/1‑124 (MQ=255)
                                                                                                                     aaaCAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCAt                                <  4:120031/149‑1 (MQ=255)
                                                                                                                     aaaCAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCAt                                >  3:34909/1‑149 (MQ=255)
                                                                                                                      aacaacGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATg                               <  3:114684/149‑1 (MQ=255)
                                                                                                                      aacaacGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATg                               >  2:784758/1‑149 (MQ=255)
                                                                                                                      aacaacGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGACTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATg                               >  4:690966/1‑149 (MQ=255)
                                                                                                                         aacGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCa                            >  3:1003594/1‑149 (MQ=255)
                                                                                                                         aacGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCa                            >  2:435985/1‑149 (MQ=255)
                                                                                                                              gCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCaa                       >  1:840935/1‑149 (MQ=255)
                                                                                                                              gCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCaa                       >  2:98272/1‑149 (MQ=255)
                                                                                                                                cAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAata                     <  3:431886/149‑1 (MQ=255)
                                                                                                                                 taaaaaaaTACCGGCATAACGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAatat                    >  3:624583/4‑149 (MQ=255)
                                                                                                                                 taaaaaaaTACCGGCATAACGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAatat                    >  1:467659/4‑149 (MQ=255)
                                                                                                                                  aTAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAAtatt                   >  3:63986/1‑149 (MQ=255)
                                                                                                                                  aTAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAAAGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAAtatt                   >  2:990455/1‑149 (MQ=255)
                                                                                                                                     aaaaTACCGGCGTAACGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAATCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATc                >  1:557205/1‑149 (MQ=255)
                                                                                                                                      aaaTACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATcc               >  2:552853/1‑149 (MQ=255)
                                                                                                                                      aaaTACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATcc               >  1:1015986/1‑149 (MQ=255)
                                                                                                                                        aTACCGGCATAACGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgcagc                                                                 >  2:846149/1‑97 (MQ=39)
                                                                                                                                        aTACCGGCATAACGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgcagc                                                                 <  1:846149/97‑1 (MQ=39)
                                                                                                                                         tACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATCCCgg            >  2:444013/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATCCCGGTGTGCAATGG  >  NC_000913/1113400‑1113695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: