Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,169,384 G→A 8.5% intergenic (+54/+28) bhsA → / ← ldtC biofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,169,3840GA 8.5% 322.9 / 14.1 105intergenic (+54/+28)bhsA/ldtCbiofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein
Reads supporting (aligned to +/- strand):  ref base G (52/44);  new base A (5/4);  total (57/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 108 total aligned reads displayed.

AAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATT  >  NC_000913/1169239‑1169532
                                                                                                                                                 |                                                                                                                                                    
aaaaaGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCgtgt                                                                                                                                                   >  1:109186/1‑149 (MQ=255)
aaaaaGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCgtgt                                                                                                                                                   >  4:1010476/1‑149 (MQ=255)
   aaGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTcgc                                                                                                                                                >  3:461891/1‑149 (MQ=255)
    aGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTcgcg                                                                                                                                               >  3:25987/1‑149 (MQ=255)
    aGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTcgcg                                                                                                                                               >  3:1038610/1‑149 (MQ=255)
     gCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTCGCgg                                                                                                                                              <  1:792421/149‑1 (MQ=255)
      cGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCcgg                                                                                                                                             <  4:932997/149‑1 (MQ=255)
      cGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTCGCggg                                                                                                                                             <  4:346495/149‑1 (MQ=255)
        gATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGa                                                                                                                                           >  3:631190/1‑149 (MQ=255)
        gATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTCGCGGGGa                                                                                                                                           <  1:812338/149‑1 (MQ=255)
         aTGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAt                                                                                                                                          >  2:33424/1‑149 (MQ=255)
          tGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAtt                                                                                                                                         <  3:1027312/149‑1 (MQ=255)
             gaTGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAttttt                                                                                                                                      <  2:54715/149‑1 (MQ=255)
              aTGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAtttttt                                                                                                                                     <  3:818565/149‑1 (MQ=255)
                   cgcAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATcc                                                                                                                                <  3:801353/149‑1 (MQ=255)
                      aaaaTCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCggg                                                                                                                             <  4:278455/149‑1 (MQ=255)
                           cTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTa                                                                                                                        <  1:33424/149‑1 (MQ=255)
                            tttCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTAc                                                                                                                       >  1:979196/1‑149 (MQ=255)
                             ttCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACa                                                                                                                      <  2:154368/149‑1 (MQ=255)
                             ttCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACa                                                                                                                      <  1:806295/149‑1 (MQ=255)
                               ccGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGc                                                                                                                    <  4:267912/149‑1 (MQ=255)
                                   aTTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGttt                                                                                                                <  1:288442/149‑1 (MQ=255)
                                      aCTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGCCATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTtgtg                                                                                                             <  4:767590/149‑1 (MQ=255)
                                       cTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTgg                                                                                                            <  2:596754/149‑1 (MQ=255)
                                       cTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTgg                                                                                                            <  4:800260/149‑1 (MQ=255)
                                       cTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTgg                                                                                                            <  3:573946/149‑1 (MQ=255)
                                        ttCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTggg                                                                                                           <  4:370521/149‑1 (MQ=255)
                                        ttCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTggg                                                                                                           >  4:150879/1‑149 (MQ=255)
                                           tGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTc                                                                                                        >  2:432550/1‑149 (MQ=255)
                                             tAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCAc                                                                                                      >  4:14689/1‑149 (MQ=255)
                                               aCCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACtt                                                                                                    >  4:75081/1‑149 (MQ=255)
                                                ccGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtg                                                                                                   <  2:438592/149‑1 (MQ=255)
                                                  ggTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtgat                                                                                                 <  2:234255/149‑1 (MQ=255)
                                                  ggTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtgat                                                                                                 <  2:344375/149‑1 (MQ=255)
                                                   gTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtgatg                                                                                                >  2:498682/1‑149 (MQ=255)
                                                       gAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcgg                                                                                            >  1:26520/1‑149 (MQ=255)
                                                           aCCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGa                                                                                        <  2:109186/149‑1 (MQ=255)
                                                           aCCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGa                                                                                        <  2:992703/149‑1 (MQ=255)
                                                            cccTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGAc                                                                                       >  4:847365/1‑149 (MQ=255)
                                                             ccTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACa                                                                                      >  2:261037/1‑149 (MQ=255)
                                                             ccTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACa                                                                                      >  1:504939/1‑149 (MQ=255)
                                                             ccTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACa                                                                                      >  3:442922/1‑149 (MQ=255)
                                                              cTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGTTTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACAt                                                                                     >  1:485575/1‑149 (MQ=255)
                                                              cTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACAt                                                                                     >  4:907720/1‑149 (MQ=255)
                                                               tCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGTCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATc                                                                                    >  1:110587/1‑149 (MQ=255)
                                                               tCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTTGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATc                                                                                    >  2:717924/1‑149 (MQ=255)
                                                               tCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATc                                                                                    >  3:610812/1‑149 (MQ=255)
                                                                    ggAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCg                                                                               >  1:245576/1‑149 (MQ=255)
                                                                    ggAACAGCAGTAATGTATATATTGCCATTAACCCTCATTACTGCCTGCTACCGCTGCTTTTTTCCCCGCGACTTGCCGTGTCGTGGGGATTTTTTTATCCGCGCTTTACAGCGTTTGTGGGCTCCCTTGATGAGGGCGGACAGACACCg                                                                               <  2:99963/149‑1 (MQ=255)
                                                                      aaCAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGc                                                                             >  1:650784/1‑149 (MQ=255)
                                                                            agTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCg                                                                       >  1:263408/1‑149 (MQ=255)
                                                                                aTTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAAc                                                                   <  2:643512/149‑1 (MQ=255)
                                                                                   tataAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAtttttt                                                                                                                                     >  1:25466/1‑80 (MQ=255)
                                                                                   tataAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAtttttt                                                                                                                                     <  2:25466/80‑1 (MQ=255)
                                                                                   tataAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGa                                                                <  2:870190/149‑1 (MQ=255)
                                                                                   tataAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGGTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGa                                                                >  1:146844/1‑149 (MQ=255)
                                                                                        ataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatcc                                                           >  2:209398/1‑149 (MQ=255)
                                                                                         taaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatcca                                                          <  3:646249/149‑1 (MQ=255)
                                                                                           aGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatccatc                                                        <  3:564016/149‑1 (MQ=255)
                                                                                            gCAATAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATca                                                       <  2:485575/149‑1 (MQ=255)
                                                                                              aTTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGc                                                                                                                            <  4:73645/78‑1 (MQ=255)
                                                                                              aTTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGc                                                                                                                            >  3:73645/1‑78 (MQ=255)
                                                                                                 aaCCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGt                                                  <  4:599047/149‑1 (MQ=255)
                                                                                                  aCCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTTTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGtt                                                 >  4:524080/1‑149 (MQ=255)
                                                                                                    ccTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATGTGTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACTGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGc                                               >  4:281473/1‑149 (MQ=255)
                                                                                                     cTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCa                                              >  3:660119/1‑149 (MQ=255)
                                                                                                     cTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCa                                              >  1:725260/1‑149 (MQ=255)
                                                                                                      tCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATcaca                                                     >  3:268766/1‑141 (MQ=255)
                                                                                                      tCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATcaca                                                     <  4:268766/141‑1 (MQ=255)
                                                                                                      tCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCAt                                             >  4:832479/1‑149 (MQ=255)
                                                                                                      tCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCAt                                             >  3:252525/1‑149 (MQ=255)
                                                                                                        aTTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCa                                           >  1:1034916/1‑149 (MQ=255)
                                                                                                         ttAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCAc                                          <  2:475920/149‑1 (MQ=255)
                                                                                                         ttAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGGGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCAc                                          >  4:622102/1‑149 (MQ=255)
                                                                                                              gCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAg                                     >  2:388900/1‑149 (MQ=255)
                                                                                                               ccTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTTGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGc                                    <  1:717924/149‑1 (MQ=255)
                                                                                                               ccTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGc                                    >  1:33224/1‑149 (MQ=255)
                                                                                                               ccTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGc                                    <  1:88521/149‑1 (MQ=255)
                                                                                                                    tACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAg                               >  2:9107/1‑149 (MQ=255)
                                                                                                                      ctgctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGtt                             <  3:75081/149‑1 (MQ=255)
                                                                                                                       tgctgATTTTTTCCCCGCGAAATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGttt                            <  2:650784/149‑1 (MQ=255)
                                                                                                                         ctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTtgt                          >  3:1003461/1‑149 (MQ=255)
                                                                                                                          tgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTtgtg                         >  3:947347/1‑149 (MQ=255)
                                                                                                                           gATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGtgc                        >  2:895535/1‑149 (MQ=255)
                                                                                                                           gATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGtgc                        <  2:245576/149‑1 (MQ=255)
                                                                                                                             aaaaatCCCCGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGtgctg                      >  1:253592/6‑149 (MQ=255)
                                                                                                                                 atCCCCGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGTTTCACAGCGTTTGTGGGCTCACTTGATGACGTTGGACATCCACCGGCATCCCGGTACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGAGTGTGCTGCaac                  >  4:51676/2‑147 (MQ=255)
                                                                                                                                  tCCCCGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCt                 <  2:253592/149‑1 (MQ=255)
                                                                                                                                     ccGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTa              >  4:28073/1‑149 (MQ=255)
                                                                                                                                     ccGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTa              >  1:874269/1‑149 (MQ=255)
                                                                                                                                     ccGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTa              >  3:888082/1‑149 (MQ=255)
                                                                                                                                     ccGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTa              >  3:18131/1‑149 (MQ=255)
                                                                                                                                     ccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTa              >  4:259154/1‑149 (MQ=255)
                                                                                                                                     ccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTa              >  3:327522/1‑149 (MQ=255)
                                                                                                                                       gcgACATGCCGTGTCGCGGGGGTTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCCGCGTCAGTTTGTGTTGCATCTTTaaa            >  2:247387/1‑149 (MQ=255)
                                                                                                                                       gcgACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTaaa            <  3:281473/149‑1 (MQ=255)
                                                                                                                                            aTGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGatt       <  1:895535/149‑1 (MQ=255)
                                                                                                                                             tGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGtgct                       <  2:444266/132‑1 (MQ=255)
                                                                                                                                             tGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGtgct                       >  1:444266/1‑132 (MQ=255)
                                                                                                                                                 gtgtCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGattgcatt  >  1:577858/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
AAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATT  >  NC_000913/1169239‑1169532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: