Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,370,026 | T→A | 6.2% | intergenic (+23/‑190) | pspE → / → ycjM | thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,370,026 | 0 | T | A | 6.2% | 408.5 / 10.6 | 122 | intergenic (+23/‑190) | pspE/ycjM | thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein |
Reads supporting (aligned to +/- strand): ref base T (54/60); new base A (4/4); total (58/64) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-01 |
Only 100 of 127 total aligned reads displayed.
AAGTGTATTGCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGA > NC_000913/1369879‑1370173 | aaGTGTATTGCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCtt > 4:498102/1‑149 (MQ=255) aaGTGTATTGCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCtt < 4:790918/149‑1 (MQ=255) aaGTGTATTGCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTGAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCtt < 4:141828/149‑1 (MQ=255) gtgtATTGCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTAt < 3:640642/149‑1 (MQ=255) tgtATTGCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATg > 4:210709/1‑149 (MQ=255) gtATTGCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATgc < 1:1010188/149‑1 (MQ=255) gCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑t < 4:278059/149‑1 (MQ=255) aaTGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑ttt < 2:53902/149‑1 (MQ=255) aaTGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑ttt > 3:911653/1‑149 (MQ=255) aaTGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑ttt > 3:954299/1‑149 (MQ=255) aTGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑tttt < 3:568971/149‑1 (MQ=255) aTGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCATAAGCGCCgctca < 3:769686/149‑5 (MQ=255) tGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑ttttt < 4:200858/149‑1 (MQ=255) gCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑tttttt < 1:287483/149‑1 (MQ=255) gCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑tttttt > 3:314180/1‑149 (MQ=255) gCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑tttttt < 1:36157/149‑1 (MQ=255) ccGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCATAAGCGCCgctcatgg < 4:408364/149‑8 (MQ=255) cGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑tttttttt < 3:876843/149‑1 (MQ=255) gACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAGCGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTc < 4:858874/149‑1 (MQ=255) gACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTc < 1:904173/149‑1 (MQ=255) aCGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCt < 4:702778/149‑1 (MQ=255) gCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑Gt < 2:702243/149‑1 (MQ=255) cAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTca > 3:389636/1‑149 (MQ=255) cAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGCTGAGAACGCCGGGGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGGTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTGTTCT‑GTca > 2:498689/1‑149 (MQ=255) caagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTAtt < 3:611604/149‑1 (MQ=255) aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTAttt > 1:885453/1‑149 (MQ=255) aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTAttt > 1:53707/1‑149 (MQ=255) aagcaaAAGAGAGCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCAGGAGCGGCGCTTATGCGCCGTTTTTTTTTTCT‑GTCACACCTTAtt < 1:996742/149‑1 (MQ=255) gagaTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATcccc < 4:293905/149‑1 (MQ=255) gaTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCAt < 4:389636/149‑1 (MQ=255) aTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACTATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATa < 4:257062/149‑1 (MQ=255) tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAg > 1:603182/1‑149 (MQ=255) ttAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTAt > 1:1054562/1‑103 (MQ=255) ttAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTAt < 2:1054562/103‑1 (MQ=255) ttAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATTGatt > 2:300436/1‑149 (MQ=255) cGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGattatt < 1:1053798/149‑1 (MQ=255) cGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTAttt < 3:180934/149‑1 (MQ=255) cGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTAttt < 2:781195/149‑1 (MQ=255) aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCtt < 4:181884/93‑1 (MQ=255) gggATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCa > 1:779069/1‑149 (MQ=255) ggATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAg < 2:932411/149‑1 (MQ=255) gATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGc < 3:780170/149‑1 (MQ=255) aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATa < 4:1034259/149‑1 (MQ=255) aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATa > 3:695589/1‑149 (MQ=255) aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATa > 2:805575/1‑149 (MQ=255) gaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCtttt > 2:956211/1‑149 (MQ=255) aCGCCGTTGGCTTGAACGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGCTCGTCCCTTATGCGCCG‑TTTTTTTTTCT‑ATCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑ACATACGCCTTTTc < 2:1051709/149‑1 (MQ=255) gCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTAtt < 1:22277/101‑1 (MQ=255) gCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTAtt > 2:22277/1‑101 (MQ=255) ccGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCt < 4:722276/149‑1 (MQ=255) cGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTg < 1:459322/149‑1 (MQ=255) ggCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTaa > 1:248003/1‑149 (MQ=255) cTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTaaaaa > 3:366574/1‑149 (MQ=255) tGAAAGACATCGCGATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTaaaaaa < 4:273619/149‑1 (MQ=255) tGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTaaaaaa < 1:331720/149‑1 (MQ=255) gACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGAtt > 4:481563/1‑149 (MQ=255) cATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTc < 1:830161/149‑1 (MQ=255) aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGTGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTcc > 4:691939/1‑149 (MQ=255) aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTc < 1:486510/148‑1 (MQ=255) aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTc > 2:486510/1‑148 (MQ=255) tCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCt > 3:397129/1‑149 (MQ=255) tCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCt < 2:885453/149‑1 (MQ=255) cAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCg < 4:386022/149‑1 (MQ=255) tGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGa < 3:749918/149‑1 (MQ=255) gCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTTTGGCCACACCTTATTAACACCCCCTTAGATTATTGGTGTC‑GCCCACAAAAAACCTTTTTTCCCTGTAAAAAATTGTTTTCCTGcttac > 4:492634/1‑145 (MQ=255) cGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTa > 2:902629/1‑149 (MQ=255) gAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTaa > 4:532058/1‑149 (MQ=255) cAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCt > 2:751744/1‑149 (MQ=255) aaaGGTTAAAAGAATTGACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTGTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTg < 3:654302/115‑1 (MQ=255) aaaGGTTAAAAGAATTCCCCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCtt < 3:822131/149‑1 (MQ=255) aaaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCtt > 1:582067/1‑149 (MQ=255) aaaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCtt < 4:933844/149‑1 (MQ=255) aaaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCtt < 1:751744/149‑1 (MQ=255) aaaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCACCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTg > 4:654302/1‑115 (MQ=255) gTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTaaaa > 4:1032426/1‑140 (MQ=255) gTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTaaaa < 3:1032426/140‑1 (MQ=255) ttAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt > 3:497063/1‑149 (MQ=255) aaaaGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTtgt < 1:1006848/144‑1 (MQ=255) aaaaGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCa > 3:112759/1‑149 (MQ=255) aaaaGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTAATTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCtttt > 2:1006848/1‑142 (MQ=255) aaaGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCaa > 1:634159/1‑149 (MQ=255) aaGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAAt < 2:1063953/149‑1 (MQ=255) aaGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAAt < 1:158633/149‑1 (MQ=255) aaGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAAt > 1:431598/1‑149 (MQ=255) gAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGT‑AAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTg > 1:72843/1‑149 (MQ=255) gAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCacac < 1:173714/48‑1 (MQ=255) gAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTg > 4:23273/1‑149 (MQ=255) aTGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAt > 1:309677/1‑149 (MQ=255) aTGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAt > 1:715395/1‑149 (MQ=255) tGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAtt > 1:53231/1‑149 (MQ=255) tGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAtt < 1:956211/149‑1 (MQ=255) aacggcgCATAAGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAtttt > 2:397742/3‑149 (MQ=255) aacggcgCATAAGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAtttt > 2:792676/3‑149 (MQ=255) aacggcgCATAAGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAtttt > 3:926308/3‑149 (MQ=255) gcggcgCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTc < 2:103156/149‑1 (MQ=255) gcgcTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGc > 2:347114/1‑149 (MQ=255) gcTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCgg > 3:1004682/1‑149 (MQ=255) cTTATGCGCCGTTTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGT‑AAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGa < 2:72843/149‑1 (MQ=255) cTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGa < 3:481563/149‑1 (MQ=255) cTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGa < 2:715395/149‑1 (MQ=255) | AAGTGTATTGCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAC‑AAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGA > NC_000913/1369879‑1370173 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |