Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,603,735 G→T 6.5% G239G (GGG→GGT lsrC → autoinducer 2 import system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,603,7350GT 6.5% 364.7 / 9.1 106G239G (GGG→GGTlsrCautoinducer 2 import system permease protein
Reads supporting (aligned to +/- strand):  ref base G (46/53);  new base T (2/5);  total (48/58)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

CGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGT  >  NC_000913/1603588‑1603880
                                                                                                                                                   |                                                                                                                                                 
cGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGc                                                                                                                                                  <  3:177136/149‑1 (MQ=255)
 gATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGCCCGGTACCGGTCt                                                                                                                                                 >  4:670543/1‑149 (MQ=255)
 gATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCt                                                                                                                                                 >  2:919443/1‑149 (MQ=255)
 gATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCt                                                                                                                                                 >  1:469923/1‑149 (MQ=255)
  aTAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTg                                                                                                                                                <  2:1015060/149‑1 (MQ=255)
     aTTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGCCCGGTACCGGTCTggtt                                                                                                                                             <  2:3667/149‑3 (MQ=255)
     aTTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGCCCGGTACCGGTCTggtt                                                                                                                                             <  4:72245/149‑3 (MQ=255)
       ttACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGCCCGGTACCGGTCTggttgg                                                                                                                                           <  3:670543/149‑5 (MQ=255)
       ttACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGAGCTGGAGAt                                                                                                                                           <  1:222678/149‑1 (MQ=255)
       ttACAGGGCGCTCGGCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCGCCAGCCAGACCGGTACCGGGCTGGAGAt                                                                                                                                           <  2:188837/149‑1 (MQ=255)
        tACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGCCCGGTACCGGTCTggttggg                                                                                                                                          <  3:670896/149‑6 (MQ=255)
        tACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATg                                                                                                                                          <  2:981585/149‑1 (MQ=255)
           aGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGaaa                                                                                                                                       <  4:340831/149‑1 (MQ=255)
           aGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGaaa                                                                                                                                       >  4:763109/1‑149 (MQ=255)
           aGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGaaa                                                                                                                                       <  4:615870/149‑1 (MQ=255)
           aGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGaaa                                                                                                                                       <  2:720361/149‑1 (MQ=255)
           aGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGaaa                                                                                                                                       <  1:1021134/149‑1 (MQ=255)
           aGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCAGGCTGGAGATGaaa                                                                                                                                       <  3:75237/149‑1 (MQ=255)
                gcTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAAt                                                                                                                                  <  1:610836/149‑1 (MQ=255)
                  tCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGACTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTg                                                                                                                                >  1:446740/1‑149 (MQ=255)
                    gTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCa                                                                                                                              <  2:688086/149‑1 (MQ=255)
                        aCTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCt                                                                                                                          <  2:493413/149‑1 (MQ=255)
                            ggCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGt                                                                                                                      >  1:788679/1‑149 (MQ=255)
                                     aCTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGggcgg                                                                                                             <  1:184664/149‑1 (MQ=255)
                                       tGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGAt                                                                                                                                           >  2:160748/1‑117 (MQ=255)
                                       tGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGAt                                                                                                                                           <  1:160748/117‑1 (MQ=255)
                                       tGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCa                                                                                                           >  3:178388/1‑149 (MQ=255)
                                       tGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCa                                                                                                           >  2:710859/1‑149 (MQ=255)
                                       tGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGCAATTGTGTTTGCTTCGCGGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATCAAAGCAATTTCAGCCTCCGTGCCGGGCGGCa                                                                                                           >  3:650891/1‑149 (MQ=255)
                                        gAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTg                                                                                                                                                <  2:861598/111‑1 (MQ=255)
                                        gAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTg                                                                                                                                                >  1:861598/1‑111 (MQ=255)
                                        gAAGCCATTCGCATTGTGGCATTTTCGTTAAACGGCTGCATGGCGGCACTGGCGGGAATTCTGTTTGCTTAGCAGATCGGTTTTATCCCCATCCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCaa                                                                                                          <  2:696579/149‑2 (MQ=255)
                                        gAAGCCATTCGCATGGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGGATTGTGTTTGCTTCGCCGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGCGCTGAAAGCAATTGCAACCTGCGTGCTGGGCGGCAt                                                                                                          >  2:909150/1‑149 (MQ=255)
                                         aaGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCAtt                                                                                                         >  2:243484/1‑149 (MQ=255)
                                          aGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTa                                                                                                        <  3:146961/149‑1 (MQ=255)
                                          aGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTa                                                                                                        <  3:763109/149‑1 (MQ=255)
                                          aGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTa                                                                                                        <  2:651880/149‑1 (MQ=255)
                                           gCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAg                                                                                                       >  2:984519/1‑149 (MQ=255)
                                            ccATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGt                                                                                                      >  3:1003779/1‑149 (MQ=255)
                                              aTTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGttt                                                                                                    >  1:424956/1‑149 (MQ=255)
                                               atCGCATTGTGGCATTTTCGTTGAACGGCGGCATGGCGGCACTGGCGCGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCGACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTCCAGCCTGCGTGCTGGGCGGCATTAGTTTg                                                                                                   <  2:469070/148‑1 (MQ=255)
                                                tCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGc                                                                                                  <  3:740927/149‑1 (MQ=255)
                                                    aTTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCgg                                                                                              <  3:240258/149‑1 (MQ=255)
                                                     ttGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGttt                                                                                                    >  1:306305/1‑142 (MQ=255)
                                                     ttGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGttt                                                                                                    <  2:306305/142‑1 (MQ=255)
                                                     ttGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCggt                                                                                             <  3:89285/149‑1 (MQ=255)
                                                          gCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTc                                                                                        <  1:984519/149‑1 (MQ=255)
                                                          gCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTc                                                                                        >  1:862167/1‑149 (MQ=255)
                                                                tCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCAGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGc                                                                                  <  1:297741/149‑1 (MQ=255)
                                                                   ttGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGAt                                                                               >  1:54341/1‑149 (MQ=255)
                                                                     gAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCa                                                                             >  2:600154/1‑149 (MQ=255)
                                                                     gAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCa                                                                             <  3:754110/149‑1 (MQ=255)
                                                                       aCGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGttt                                                                                                    <  1:683435/124‑1 (MQ=255)
                                                                       aCGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGttt                                                                                                    >  2:683435/1‑124 (MQ=255)
                                                                          gCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGt                                                                        <  4:810679/149‑1 (MQ=255)
                                                                               aTGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTggc                                                                                                                                              >  4:682525/1‑73 (MQ=255)
                                                                               aTGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGga                                                                                                                                              <  3:682525/74‑1 (MQ=255)
                                                                                      cACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGTCATTAGTTTGCTCGGTGGtt                                                                                         <  4:221225/120‑1 (MQ=255)
                                                                                      cACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGTCATTAGTTTGCTCGGTGGtt                                                                                         >  3:221225/1‑120 (MQ=255)
                                                                                           gCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATCGGTGCGGTACTCGGCGCATg                                                       >  4:757961/1‑149 (MQ=255)
                                                                                             gggAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGt                                                     >  1:826553/1‑149 (MQ=255)
                                                                                             gggAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGt                                                     >  1:307909/1‑149 (MQ=255)
                                                                                               gAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTc                                                   >  3:1022549/1‑149 (MQ=255)
                                                                                                aaTTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAAtt                                                                                                                                 <  1:894712/70‑1 (MQ=255)
                                                                                                aaTTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAAtt                                                                                                                                 >  2:894712/1‑70 (MQ=255)
                                                                                                aaTTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTcc                                                  <  2:119622/149‑1 (MQ=255)
                                                                                                   tgtgTTTGCTTCGCAGATTGGTTTTGTCCTCAACCATACCGGTACCGGGCTGGAGATGACAGCCATTGCAGCCTACGTGCCGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGTGATCCTTGGTGCGGTACTCAGCGCATGGTTCCTGa                                               <  4:650891/149‑1 (MQ=255)
                                                                                                    gtgtTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGAc                                              <  2:424956/149‑1 (MQ=255)
                                                                                                     tgtTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACg                                             >  3:545107/1‑149 (MQ=255)
                                                                                                       tttGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCa                                           >  3:207903/1‑149 (MQ=255)
                                                                                                         tGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGa                                         <  1:238535/149‑1 (MQ=255)
                                                                                                         tGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGa                                         <  3:952654/149‑1 (MQ=255)
                                                                                                            ttCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCg                                      >  1:834791/1‑149 (MQ=255)
                                                                                                               gCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATa                                   <  1:919443/149‑1 (MQ=255)
                                                                                                                 aGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGc                                 <  2:834791/149‑1 (MQ=255)
                                                                                                                   aTTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGt                               >  1:237040/1‑149 (MQ=255)
                                                                                                                       gTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTg                           >  4:725105/1‑149 (MQ=255)
                                                                                                                        ttttATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCAGTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTgg                          >  1:144593/1‑149 (MQ=255)
                                                                                                                          ttATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTg                        <  3:230063/149‑1 (MQ=255)
                                                                                                                            aTCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTgg                          <  3:858622/145‑1 (MQ=255)
                                                                                                                            aTCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTgg                          >  4:858622/1‑145 (MQ=255)
                                                                                                                              ccccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGt                    >  2:707428/1‑149 (MQ=255)
                                                                                                                               cccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGtt                   >  4:894688/1‑149 (MQ=255)
                                                                                                                               cccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGtt                   >  2:471098/1‑149 (MQ=255)
                                                                                                                               cccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGtt                   >  2:770694/1‑149 (MQ=255)
                                                                                                                               cccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGtt                   >  4:484663/1‑149 (MQ=255)
                                                                                                                               cccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCAGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGtt                   >  2:312220/1‑149 (MQ=255)
                                                                                                                                ccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGa                                               <  4:69690/120‑1 (MQ=255)
                                                                                                                                ccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGa                                               >  3:69690/1‑120 (MQ=255)
                                                                                                                                ccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTg                  >  3:157409/1‑149 (MQ=255)
                                                                                                                                ccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTg                  >  3:1011088/1‑149 (MQ=255)
                                                                                                                                ccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTg                  >  2:714776/1‑149 (MQ=255)
                                                                                                                                 cAATCAGCCCGGTATCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGTGTCATTAGTTTGCTGGGTGGTTCCGGTGCGTTCATTGGTGCGGTACTCGTCGCATGGTTCCGGACGCAGATCGATAGCGTACTGGTGCTGTTgc                 <  4:852812/149‑1 (MQ=255)
                                                                                                                                 cAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTgc                 <  1:243484/149‑1 (MQ=255)
                                                                                                                                 cAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTgc                 <  3:869265/149‑1 (MQ=255)
                                                                                                                                     cAGCCCGGTACCGGTCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCAt             >  2:208123/1‑149 (MQ=255)
                                                                                                                                        aCCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTcc          <  4:1022549/149‑1 (MQ=255)
                                                                                                                                         ccGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCg         <  3:34220/149‑1 (MQ=255)
                                                                                                                                           ggTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCGGTTGCGCATTCCGGc       <  4:1003779/149‑1 (MQ=255)
                                                                                                                                                cGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCAtggt  <  3:950546/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGT  >  NC_000913/1603588‑1603880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: