Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,863,802 C→A 6.8% intergenic (+36/‑48) gapA → / → yeaD glyceraldehyde‑3‑phosphate dehydrogenase A/D‑hexose‑6‑phosphate epimerase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,863,8020CA 6.8% 454.2 / 17.3 132intergenic (+36/‑48)gapA/yeaDglyceraldehyde‑3‑phosphate dehydrogenase A/D‑hexose‑6‑phosphate epimerase‑like protein
Reads supporting (aligned to +/- strand):  ref base C (63/60);  new base A (3/6);  total (66/66)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.13e-01

Only 100 of 132 total aligned reads displayed.

AAAGCTGGTATCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGATCAT  >  NC_000913/1863656‑1863948
                                                                                                                                                  |                                                                                                                                                  
        tATCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACCGAAGTCGCTCtt                                                                                                                                          <  2:1048816/149‑1 (MQ=255)
         aTCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttt                                                                                                                                         <  2:178727/149‑1 (MQ=255)
         aTCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttt                                                                                                                                         <  2:290836/149‑1 (MQ=255)
         aTCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACCGAAGTCGCTCttt                                                                                                                                         <  3:510016/149‑1 (MQ=255)
          tCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCtttt                                                                                                                                        >  4:301318/1‑149 (MQ=255)
          tCGCTCTGAACGACAACTTCGTGAAACTAGTCTCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCCAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCtttt                                                                                                                                        >  4:643927/1‑149 (MQ=255)
           cGCTCTGAACGACAACTTCGTGAAACTTGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttttt                                                                                                                                       <  3:724647/149‑1 (MQ=255)
           cGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttttt                                                                                                                                       <  4:614368/149‑1 (MQ=255)
           cGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttttt                                                                                                                                       >  3:829726/1‑149 (MQ=255)
           cGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGCTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACCGAAGTCGCTCtttta                                                                                                                                       <  1:187648/149‑2 (MQ=255)
            gCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATAGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCtttttt                                                                                                                                      >  2:906428/1‑149 (MQ=255)
            gCTCTGAAAGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACCGAAGTCGCTCttttta                                                                                                                                      <  2:317818/149‑2 (MQ=255)
             ctctGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttttttt                                                                                                                                     <  4:1014179/149‑1 (MQ=255)
             ctctGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttttttt                                                                                                                                     >  3:587829/1‑149 (MQ=255)
             ctctGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttttttt                                                                                                                                     <  3:89636/149‑1 (MQ=255)
                     gACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGAt                                                                                                                             >  2:621771/1‑149 (MQ=255)
                           ttCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATg                                                                                                                       >  3:588044/1‑149 (MQ=255)
                           ttCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATg                                                                                                                       >  4:610047/1‑149 (MQ=255)
                            tCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGAACGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGa                                                                                                                      <  4:923837/149‑1 (MQ=255)
                             cGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGaa                                                                                                                     <  3:522444/149‑1 (MQ=255)
                              gTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAg                                                                                                                    <  1:980493/149‑1 (MQ=255)
                                gAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGtt                                                                                                                  <  3:804114/149‑1 (MQ=255)
                                   aCTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTaaa                                                                                                               <  3:993488/149‑1 (MQ=255)
                                    ttGGTATCCTGGTACGACAACGAAAGCGGCTACTACAACTAAGTTCTGGACCTGAGCGCTCACAGCTCCACATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCCTTTATTTACCTGATAAAATGAAGTTAAAg                                                                                                              <  4:956250/148‑1 (MQ=255)
                                    cTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGAACTGATCGCTCACAACTCCAAATAAGTTGAGATTACACTGTGATCTAAAAAGAGCGACCTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAg                                                                                                              <  4:636803/149‑1 (MQ=255)
                                     tGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAgg                                                                                                             >  3:784368/1‑149 (MQ=255)
                                        tATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACt                                                                                                          >  3:976304/1‑149 (MQ=255)
                                              ggTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCa                                                                                                    >  2:511416/1‑149 (MQ=255)
                                               gTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCAt                                                                                                   <  4:784368/149‑1 (MQ=255)
                                                      aaCGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAg                                                                                            >  2:283043/1‑149 (MQ=255)
                                                       aCGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGa                                                                                           >  2:878099/1‑149 (MQ=255)
                                                         gAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGaaa                                                                                         >  3:588347/1‑149 (MQ=255)
                                                               ggTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAAttttt                                                                                   <  1:579929/149‑1 (MQ=255)
                                                                gTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGaa                                                                                                                     >  3:280041/1‑114 (MQ=255)
                                                                gTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGaa                                                                                                                     <  4:280041/114‑1 (MQ=255)
                                                                gTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTg                                                                                  >  3:290790/1‑149 (MQ=255)
                                                                 ttACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGc                                                                                 <  4:136843/149‑1 (MQ=255)
                                                                     tCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCtt                                                                             >  1:996508/1‑149 (MQ=255)
                                                                     tCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCtt                                                                             >  4:435775/1‑149 (MQ=255)
                                                                       caacaaAGTTCTGGACCTGATCTCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGGCATGATTAAGAAAATTTTTGCCCTTcc                                                                           >  1:255328/1‑149 (MQ=255)
                                                                       caacaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTcc                                                                           >  3:553905/1‑149 (MQ=255)
                                                                        aacaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCg                                                                          >  2:393357/1‑149 (MQ=255)
                                                                         acaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTaa                                                                                             <  3:501626/129‑1 (MQ=255)
                                                                         acaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTaa                                                                                             >  4:501626/1‑129 (MQ=255)
                                                                         acaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCgg                                                                         >  3:988818/1‑149 (MQ=255)
                                                                         acaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCgg                                                                         >  2:208859/1‑149 (MQ=255)
                                                                           aaaGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTc                                                                       >  1:493415/1‑149 (MQ=255)
                                                                           aaaGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACCGAAGTCGCTCttt                                                                                                                                         >  4:711903/1‑83 (MQ=38)
                                                                             aGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCAt                                                                     <  1:878099/149‑1 (MQ=255)
                                                                              gTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATc                                                                    <  1:683700/149‑1 (MQ=255)
                                                                                     aCCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACaaa                                                             <  1:621771/149‑1 (MQ=255)
                                                                                      ccTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAAt                                                            >  2:709655/1‑149 (MQ=255)
                                                                                          aTCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATCATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTcc                                                        <  3:643927/149‑1 (MQ=255)
                                                                                           tCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTccc                                                       <  1:393357/149‑1 (MQ=255)
                                                                                            cGCTCACATCTCCAAATAAGTTGATATGACACTGTGCTCTAAAAAGAGCGACTTCGATAGCTCTTTTTTTTACCTAAAAAAATGACGTTAAAGAACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTcccc                                                      <  3:1032965/149‑1 (MQ=255)
                                                                                              cTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTg                                                    >  4:151746/1‑149 (MQ=255)
                                                                                                cacaTCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTc                                                  <  1:751787/149‑1 (MQ=255)
                                                                                                 acaTCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTcc                                                 >  2:179660/1‑149 (MQ=255)
                                                                                                   aTCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACCGAAGTCGCTCttt                                                                                                                                         >  3:793204/1‑59 (MQ=25)
                                                                                                   aTCTCCAAATAAGTCGAGATGACACTGTGATCTAAAAAGAGCGACCGAAGTCGCTCttt                                                                                                                                         <  4:793204/59‑1 (MQ=21)
                                                                                                     ctcCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCtctc                                             <  2:712680/149‑1 (MQ=255)
                                                                                                     ctcCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCtctc                                             >  1:812832/1‑149 (MQ=255)
                                                                                                      tcCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTcc                                                                           <  2:828392/118‑1 (MQ=255)
                                                                                                         aaaTAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgt                                         >  1:918007/1‑149 (MQ=255)
                                                                                                         aaaTAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgt                                         <  3:560491/149‑1 (MQ=255)
                                                                                                         aaaTAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgt                                         >  4:560491/1‑149 (MQ=255)
                                                                                                          aaTAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgtc                                        <  1:383901/149‑1 (MQ=255)
                                                                                                          aaTAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgtc                                        <  3:301318/149‑1 (MQ=255)
                                                                                                                ttGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAAc                                  >  4:47807/1‑149 (MQ=255)
                                                                                                                    gaTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCtttttt                                                                                                                                      >  1:27442/1‑45 (MQ=255)
                                                                                                                    gaTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGa                              <  1:118369/149‑1 (MQ=255)
                                                                                                                    gaTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGa                              <  3:423243/149‑1 (MQ=255)
                                                                                                                    gaTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGa                              <  3:318793/149‑1 (MQ=255)
                                                                                                                    gaTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCACTGTCCTCTCCCGTCGTAAACTGGa                              >  3:688578/1‑149 (MQ=255)
                                                                                                                     aTGACACTGTTATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGTAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGACGTAAACTGGAt                             <  2:678832/149‑1 (MQ=255)
                                                                                                                     aTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGAt                             <  3:435775/149‑1 (MQ=255)
                                                                                                                       gACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGa                           >  4:958929/1‑149 (MQ=255)
                                                                                                                        acacTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGaa                          >  1:818283/1‑149 (MQ=255)
                                                                                                                        acacTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGaa                          >  2:445627/1‑149 (MQ=255)
                                                                                                                          acTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACt                        >  4:690352/1‑149 (MQ=255)
                                                                                                                            tgtgATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTgg                      >  4:357114/1‑149 (MQ=255)
                                                                                                                            tgtgATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTgg                      >  1:549403/1‑149 (MQ=255)
                                                                                                                            tgtgATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTgg                      >  4:763691/1‑149 (MQ=255)
                                                                                                                            tgtgATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTgg                      >  3:446166/1‑149 (MQ=255)
                                                                                                                             gtgATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGa                     <  4:742602/149‑1 (MQ=255)
                                                                                                                                   tAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCGCTGTTCTCTCCCGTCGTAAACTGGATGAACTGGACCTCAt               >  4:685364/1‑149 (MQ=255)
                                                                                                                                   tAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGa                              >  2:43585/1‑134 (MQ=255)
                                                                                                                                   tAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGa                              <  1:43585/134‑1 (MQ=255)
                                                                                                                                   tAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCAt               >  2:358181/1‑149 (MQ=255)
                                                                                                                                   tAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCAt               >  1:264184/1‑149 (MQ=255)
                                                                                                                                   tAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCAt               >  1:145126/1‑149 (MQ=255)
                                                                                                                                   tAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCAt               >  3:1038446/1‑149 (MQ=255)
                                                                                                                                    aaaaaGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCAtt              <  3:47807/149‑1 (MQ=255)
                                                                                                                                      aaaGAGCGACCGAAGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATtgt            >  4:167657/1‑149 (MQ=255)
                                                                                                                                          agCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTc        <  1:603992/149‑1 (MQ=255)
                                                                                                                                           gCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCg       >  4:159626/1‑149 (MQ=255)
                                                                                                                                            cGACTTCGGTCGTTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGa      <  1:107837/149‑1 (MQ=255)
                                                                                                                                              aCTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATTAACTGGACCTCATTGTGGTAGatc    <  1:906428/149‑1 (MQ=255)
                                                                                                                                              aCTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGatc    <  2:996508/149‑1 (MQ=255)
                                                                                                                                                ttCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGatcat  >  2:150409/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AAAGCTGGTATCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGATCAT  >  NC_000913/1863656‑1863948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: