Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,910,295	A→T	5.0%	D7V (GAC→GTC)	yebQ →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,910,295	0	A	T	5.0%	339.6 / 7.2	99	D7V (GAC→GTC)	yebQ	putative transporter
Reads supporting (aligned to +/- strand):  ref base A (48/46);  new base T (2/3);  total (50/49)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.11e-01

TTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTA  >  NC_000913/1910153‑1910443                                                                                                                                               |                                                                                                                                                     tttCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCt                                                                                                                                                >  3:78402/1‑149 (MQ=255) tttCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCt                                                                                                                                                <  1:542513/149‑1 (MQ=255)   tCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGc                                                                                                                                              <  4:48889/149‑1 (MQ=255)    cGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGTCGGCCTgaa                                                                                                                                             <  2:557994/149‑3 (MQ=255)       aCCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGTCGTCCTgaactt                                                                                                                                          <  1:830176/149‑6 (MQ=255)         cAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGc                                                                                                                                        >  2:948229/1‑149 (MQ=255)           gggTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGccc                                                                                                                                      >  1:412004/1‑149 (MQ=255)               ggtCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCGGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGc                                                                                                                                  <  4:592426/149‑1 (MQ=255)               ggtCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGc                                                                                                                                  <  4:904721/149‑1 (MQ=255)                 tCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGa                                                                                                                                >  1:654440/1‑149 (MQ=255)                       tCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGt                                                                                                                          >  4:938415/1‑149 (MQ=255)                        ccATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCCACGGCCTGCCATTGCCCCAGCGATACGGTg                                                                                                                         >  1:691408/1‑149 (MQ=255)                         cATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGccc                                                                                                                                      <  2:588185/135‑1 (MQ=255)                         cATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGccc                                                                                                                                      >  1:588185/1‑135 (MQ=255)                         cATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGc                                                                                                                        >  3:246971/1‑149 (MQ=255)                          aTCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCg                                                                                                                       >  3:689443/1‑149 (MQ=255)                          aTCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCg                                                                                                                       >  2:929318/1‑149 (MQ=255)                          aTCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCg                                                                                                                       >  1:66876/1‑149 (MQ=255)                           tCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGa                                                                                                                      <  1:136386/149‑1 (MQ=255)                             ttttGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGata                                                                                                                    <  1:623041/149‑1 (MQ=255)                                    ggTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAATATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCa                                                                                                             >  2:483649/1‑149 (MQ=255)                                      ttagttaCAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCAtt                                                                                                           <  1:353233/149‑1 (MQ=255)                                          ttaCAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGa                                                                                                       <  4:78402/149‑1 (MQ=255)                                          ttaCAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGa                                                                                                       >  1:225202/1‑149 (MQ=255)                                          ttaCAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGa                                                                                                       >  1:479678/1‑149 (MQ=255)                                            aCAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGAtt                                                                                                     <  4:584516/149‑1 (MQ=255)                                            aCAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGAtt                                                                                                     <  1:1058913/149‑1 (MQ=255)                                            aCAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGAtt                                                                                                     <  3:534562/149‑1 (MQ=255)                                              aaTTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTgg                                                                                                   >  2:443362/1‑149 (MQ=255)                                              aaTTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATAT‑AAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGt                                                                                                  <  3:342377/149‑1 (MQ=255)                                               aTTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGt                                                                                                  >  3:565404/1‑149 (MQ=255)                                                ttCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTa                                                                                                 >  4:366018/1‑149 (MQ=255)                                                ttCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTACCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTa                                                                                                 >  1:314235/1‑149 (MQ=255)                                                   tGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTAttt                                                                                              >  1:358048/1‑149 (MQ=255)                                                       aCATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGAt                                                                                          <  1:443362/149‑1 (MQ=255)                                                       aCATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGAt                                                                                          <  4:331682/149‑1 (MQ=255)                                                       aCATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGAt                                                                                          <  2:940680/149‑1 (MQ=255)                                                       aCATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGAt                                                                                          <  4:755615/149‑1 (MQ=255)                                                       aCATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGAt                                                                                          <  2:969506/149‑1 (MQ=255)                                                        cATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATg                                                                                         >  1:231083/1‑149 (MQ=255)                                                         aTCCACCGTGAATATCAGTGCTAGAATCATACCCTTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATgg                                                                                        >  4:925458/1‑149 (MQ=255)                                                         aTCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATgg                                                                                        >  3:735476/1‑149 (MQ=255)                                                           ccACCGTGAATATCAGTGCTAGAATCCTACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCTATATTAACCATTGTGATTGGTATTTCGATGGcc                                                                                      <  1:508259/149‑1 (MQ=255)                                                              ccGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTc                                                                                   >  4:837943/1‑149 (MQ=255)                                                               cGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTcc                                                                                  <  3:101366/149‑1 (MQ=255)                                                                gTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCt                                                                                 >  4:543591/1‑149 (MQ=255)                                                                   aaTATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGa                                                                              <  2:511343/149‑1 (MQ=255)                                                                   aaTATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGa                                                                              <  2:225202/149‑1 (MQ=255)                                                                   aaTATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGa                                                                              <  4:735476/149‑1 (MQ=255)                                                                      atCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTACCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACgg                                                                           >  1:821524/1‑149 (MQ=255)                                                                       tCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCt                                                                                                                                                <  3:425812/79‑1 (MQ=255)                                                                       tCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCt                                                                                                                                                >  4:425812/1‑79 (MQ=255)                                                                       tCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATgg                                                                                        <  2:694381/135‑1 (MQ=255)                                                                       tCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATgg                                                                                        >  1:694381/1‑135 (MQ=255)                                                                        cAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGgcg                                                                         >  1:96724/1‑149 (MQ=255)                                                                            gCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAAt                                                                     <  4:689443/149‑1 (MQ=255)                                                                              tAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCg                                                                   >  2:59572/1‑149 (MQ=255)                                                                                   tCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAAc                                                              <  4:602707/149‑1 (MQ=255)                                                                                         cccTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGcc                                                        >  3:937090/1‑149 (MQ=255)                                                                                         cccTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGcc                                                        >  3:893916/1‑149 (MQ=255)                                                                                         cccTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGcc                                                        >  2:172988/1‑149 (MQ=255)                                                                                              ttGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCGACGTCGCCCTGcc                                                   <  2:96724/149‑1 (MQ=255)                                                                                              ttGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGcc                                                   >  2:705979/1‑149 (MQ=255)                                                                                                gATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCcaa                                                 >  1:534690/1‑149 (MQ=255)                                                                                                     ttCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATc                                            <  3:837943/149‑1 (MQ=255)                                                                                                     ttCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATc                                            <  1:948229/149‑1 (MQ=255)                                                                                                        aCCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTg                                                                                                    >  1:1000629/1‑90 (MQ=255)                                                                                                        aCCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTg                                                                                                    <  2:1000629/90‑1 (MQ=255)                                                                                                         ccAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCa                                        >  3:647600/1‑149 (MQ=255)                                                                                                          cAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCAc                                       >  2:977445/1‑149 (MQ=255)                                                                                                          cAAAGATATAAAATTCCTATGCCAAAAGTTCACGCCGACGGCCTGCCATCGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCAc                                       <  4:853759/149‑1 (MQ=255)                                                                                                           aaaGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACg                                      >  3:185825/1‑149 (MQ=255)                                                                                                            aaGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACgg                                     >  3:3454/1‑149 (MQ=255)                                                                                                              gATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGAc                                   <  1:713677/149‑1 (MQ=255)                                                                                                                tataAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCt                                 >  2:106679/1‑149 (MQ=255)                                                                                                                   aaaaTTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCa                              >  3:649068/1‑149 (MQ=255)                                                                                                                    aaaTTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCAt                             <  2:444469/149‑1 (MQ=255)                                                                                                                     aaTTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATg                            <  4:647600/149‑1 (MQ=255)                                                                                                                     aaTTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATg                            >  2:801250/1‑149 (MQ=255)                                                                                                                      aTTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGc                           >  3:405979/1‑149 (MQ=255)                                                                                                                      aTTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGc                           >  1:303793/1‑149 (MQ=255)                                                                                                                        tCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCa                         >  4:170472/1‑149 (MQ=255)                                                                                                                          cTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCAc                                       <  1:1021636/133‑1 (MQ=255)                                                                                                                          cTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCAc                                       >  2:1021636/1‑133 (MQ=255)                                                                                                                           tATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACgc                      <  4:6363/149‑1 (MQ=255)                                                                                                                                 aaaaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACgccagcca                >  3:98555/1‑149 (MQ=255)                                                                                                                                   aatggcAGGCCGTCGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGt              >  1:446876/6‑149 (MQ=255)                                                                                                                                   aaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCCCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGt              <  4:565404/149‑1 (MQ=255)                                                                                                                                    atggcAGGCCGTCGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGtt             >  1:908143/5‑149 (MQ=255)                                                                                                                                      ttCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCAt                             <  4:487540/131‑1 (MQ=255)                                                                                                                                      ttCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCAt                             >  3:487540/1‑131 (MQ=255)                                                                                                                                        cAGGCCGTCGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCAt         <  2:446876/149‑1 (MQ=255)                                                                                                                                         aGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATc        <  1:341924/149‑1 (MQ=255)                                                                                                                                          ggCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCt       <  1:172988/149‑1 (MQ=255)                                                                                                                                            ccGACGGCCTGCCATTGTCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTgg     <  4:550802/149‑1 (MQ=255)                                                                                                                                            ccGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTgg     <  4:405979/149‑1 (MQ=255)                                                                                                                                            ccGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTgg     <  3:938415/149‑1 (MQ=255)                                                                                                                                            ccGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTgg     <  4:893574/149‑1 (MQ=255)                                                                                                                                             cGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTggg    <  2:133431/149‑1 (MQ=255)                                                                                                                                               aCGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTa  <  4:868408/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                     TTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTA  >  NC_000913/1910153‑1910443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑