| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,999,231 | T→A | 7.2% | T84S (ACT→TCT) | dcyD ← | D‑cysteine desulfhydrase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,999,231 | 0 | T | A | 7.2% | 278.8 / 10.6 | 83 | T84S (ACT→TCT) | dcyD | D‑cysteine desulfhydrase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (33/44); new base A (4/2); total (37/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01 | |||||||||||
AGTGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAAT > NC_000913/1999086‑1999378 | aGTGCGTCGCACATTTCAATCTGGGTAATGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTg > 2:453812/1‑149 (MQ=255) gTGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGACTga < 3:496918/149‑2 (MQ=255) tCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGACTGCCGcag > 4:701089/1‑148 (MQ=255) gCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACAt > 4:819302/1‑149 (MQ=255) gCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGACTGCCGcagtc < 3:701089/149‑4 (MQ=255) cacaTTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATg > 1:628584/1‑149 (MQ=255) aTTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGtt < 1:253605/149‑1 (MQ=255) aTTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGtt > 1:801520/1‑149 (MQ=255) cAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCTCATGGTTAGAc < 3:681563/149‑1 (MQ=255) cAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGAc < 4:871961/149‑1 (MQ=255) aTCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTg > 2:1045480/1‑149 (MQ=255) cTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCACTGCGGCAGTCTGCCGCACATGGTTAGa < 4:250061/149‑1 (MQ=255) tGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGAt > 4:917129/1‑149 (MQ=255) gggTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTa > 3:200275/1‑140 (MQ=255) gggTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTa < 4:200275/140‑1 (MQ=255) ggTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCg > 4:297420/1‑149 (MQ=255) gTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATg < 1:316128/134‑1 (MQ=255) gTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATg > 2:316128/1‑134 (MQ=255) tGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCg > 2:570677/1‑149 (MQ=255) gAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCgg > 2:1058587/1‑149 (MQ=255) aCAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCa < 3:368557/149‑1 (MQ=255) aCAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCa < 4:982633/149‑1 (MQ=255) aTCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTaa > 1:472786/1‑149 (MQ=255) cAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCa < 2:5071/149‑1 (MQ=255) aGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACt > 2:343804/1‑127 (MQ=255) aGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACt < 1:343804/127‑1 (MQ=255) aGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAg > 2:762607/1‑149 (MQ=255) gCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGc > 4:692980/1‑149 (MQ=255) caaGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTATGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTAt < 2:904413/149‑1 (MQ=255) gCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCgg > 4:314395/1‑149 (MQ=255) gATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCa > 4:128564/1‑149 (MQ=255) ttGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCAcc < 2:973889/149‑1 (MQ=255) ccGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTc < 1:585307/149‑1 (MQ=255) gTTGGTTAAATTGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCAc > 1:556647/1‑149 (MQ=255) gTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCAc > 3:241846/1‑149 (MQ=255) ttAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGTGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCaga < 3:106065/149‑1 (MQ=255) ttAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTACTCAGCGTATCGGCACCTTCACGCaga < 3:652451/149‑1 (MQ=255) ttAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCaga > 3:632783/1‑149 (MQ=255) ttAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCaga < 2:892275/149‑1 (MQ=255) aaTAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCa > 3:954142/1‑149 (MQ=255) tAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATc < 2:829880/149‑1 (MQ=255) gTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTg > 3:311045/1‑149 (MQ=255) tttCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCATCGTATCGGCACCTTCACGCAGAGCATCTGcc < 1:935690/149‑1 (MQ=255) tGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCga < 1:275125/149‑1 (MQ=255) gCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGGCCCGGCAGTAATCAGCGTATCGGCATCTTCACGCAGAGCATCTGCCGCgag < 1:847359/149‑1 (MQ=255) gTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGaaa > 3:845956/1‑149 (MQ=255) gTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGaaa < 4:863508/149‑1 (MQ=255) gtgCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTc < 3:35504/149‑1 (MQ=255) tgCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTcc < 3:396553/149‑1 (MQ=255) gCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCa < 3:150092/149‑1 (MQ=255) aaTAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGc > 2:958330/1‑103 (MQ=255) aaTAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGc < 1:958330/103‑1 (MQ=255) tAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTa < 4:954142/149‑1 (MQ=255) gATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATTGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCAc < 4:853287/114‑1 (MQ=255) gATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCAc > 3:853287/1‑114 (MQ=255) gATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGt < 4:148369/149‑1 (MQ=255) aTTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTa > 2:405233/1‑149 (MQ=255) ttttCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTaa < 4:935800/149‑1 (MQ=255) tttCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAAt > 1:49999/1‑149 (MQ=255) agcagcGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTAtt < 4:311045/149‑1 (MQ=255) agcagcGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTAtt > 4:568997/1‑149 (MQ=255) agcagcGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTAtt > 3:726585/1‑149 (MQ=255) agcagcGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTAtt < 2:472786/149‑1 (MQ=255) cGCAGTGCAGACCGAGTTTGACAGCG‑‑‑‑‑ACTGCGGCAGTCTGCAGCACCTGGTTAGTCTGGATCACCCCGGCAGTAATCAACGTATCGGCACTTTCACGCAGAGCATCTGCCCCGTAAAATTTGACCTTACGTAAGTTATTgccgcatatt < 4:864883/149‑6 (MQ=255) gCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTg > 1:155157/1‑149 (MQ=255) tGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCAt < 2:96619/149‑1 (MQ=255) gCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATg < 1:1021024/149‑1 (MQ=255) aGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATggg > 4:816371/1‑149 (MQ=255) gACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATgggg < 1:1000211/149‑1 (MQ=255) cGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATgggg < 2:434057/146‑1 (MQ=255) cGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATgggg > 1:434057/1‑146 (MQ=255) aGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGAc < 4:163003/149‑1 (MQ=255) aGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGAc < 3:819302/149‑1 (MQ=255) aGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGAc < 1:257585/149‑1 (MQ=255) cAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATcc > 1:1019126/1‑149 (MQ=255) aaccatgtGCGGCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATcc > 3:205180/8‑149 (MQ=255) atgtGCGGCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGtt > 4:537452/4‑149 (MQ=255) atgtGCGGCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGtt > 3:341755/4‑149 (MQ=255) aCTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGttt < 2:1054889/149‑1 (MQ=255) gCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTtgat < 4:284268/149‑1 (MQ=255) cAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGaaa < 3:314395/149‑1 (MQ=255) aGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAGATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGaaaa < 3:505804/149‑1 (MQ=255) gTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAAt < 3:816371/149‑1 (MQ=255) | AGTGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCG‑‑‑‑‑ACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAAT > NC_000913/1999086‑1999378 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |