Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,255,991 A→T 7.1% K213* (AAA→TAA) ‡ yeiL → putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,255,9910AT 7.1% 388.7 / 16.6 113K213* (AAA→TAA) ‡yeiLputative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (44/61);  new base T (4/4);  total (48/65)
Fisher's exact test for biased strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.03e-01

Only 100 of 117 total aligned reads displayed.

AGAGTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGAAAG  >  NC_000913/2255843‑2256138
                                                                                                                                                    |                                                                                                                                                   
agagTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATa                                                                                                                                                     >  4:331338/1‑149 (MQ=255)
  agTATTTAGGCGTTTCTTAGCTACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGCGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGTCGCTGGAGATGGACCCGGAGAATaaa                                                                                                                                                   <  2:547037/149‑1 (MQ=255)
     aTTTAGGCGTTTCTTATCGACATGTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATtc                                                                                                                                                <  3:48603/149‑1 (MQ=255)
      tttAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATtct                                                                                                                                               <  3:913024/149‑1 (MQ=255)
       ttAGGCGTTTCTTATCGTCATCTTTTATATGTTCTCGCGCAGTTCATTCTCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGCACTGGCGCTGGAGATGGACCCGGAGAATAAATtctc                                                                                                                                              <  4:885071/149‑1 (MQ=255)
       ttAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAACAAATtctc                                                                                                                                              <  4:91809/149‑1 (MQ=255)
        tAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTcc                                                                                                                                             <  1:672830/149‑1 (MQ=255)
         aGGAGTTTGTTATCGACATCGTTCATATGTTCTCGCGCATTTCATGCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTAATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGACGGACCCGGAGAATAAATTCTCCg                                                                                                                                            <  2:588589/149‑1 (MQ=255)
          ggCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCgg                                                                                                                                           <  1:95729/149‑1 (MQ=255)
           gCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCggg                                                                                                                                          >  3:84662/1‑149 (MQ=255)
            cGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGga                                                                                                                                         <  3:578389/149‑1 (MQ=255)
            cGTTTCTTATCGACATCTTATATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGga                                                                                                                                         <  3:412689/149‑1 (MQ=255)
             gTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGgat                                                                                                                                        >  2:333984/1‑149 (MQ=255)
             gTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGgat                                                                                                                                        <  4:456867/149‑1 (MQ=255)
               ttCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGgatga                                                                                                                                      >  3:163654/1‑149 (MQ=255)
                tCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGgatgat                                                                                                                                     <  1:640962/149‑1 (MQ=255)
                  ttATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGc                                                                                                                                   >  1:65006/1‑149 (MQ=255)
                  ttATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGc                                                                                                                                   >  3:234659/1‑149 (MQ=255)
                   tATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAGTAAATTCTCCGGGATGATGCa                                                                                                                                  <  1:223840/149‑1 (MQ=255)
                    aTCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAg                                                                                                                                 >  1:1031394/1‑149 (MQ=255)
                       gACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTaa                                                                                                                              >  3:517044/1‑149 (MQ=255)
                          aTCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTaaaaa                                                                                                                           <  1:628969/149‑1 (MQ=255)
                           tCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAat                                                                                                                          >  1:797862/1‑149 (MQ=255)
                             ttttATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCGGGATGATGCAGTAAAAatta                                                                                                                        >  1:874567/1‑149 (MQ=255)
                             ttttATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGatgatg                                                                                                                                    >  3:487052/1‑137 (MQ=255)
                             ttttATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGatgatg                                                                                                                                    <  4:487052/137‑1 (MQ=255)
                             ttttATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAatta                                                                                                                        >  3:289375/1‑149 (MQ=255)
                                 atatGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTAtttt                                                                                                                    >  2:553684/1‑149 (MQ=255)
                                  tatGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTc                                                                                                                   >  3:331398/1‑149 (MQ=255)
                                   atGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCa                                                                                                                  <  1:630938/149‑1 (MQ=255)
                                   atGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCa                                                                                                                  >  3:48883/1‑149 (MQ=255)
                                    tGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCaa                                                                                                                 >  3:241413/1‑149 (MQ=255)
                                        ctcGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAgc                                                                                                             <  1:194436/149‑1 (MQ=255)
                                        ctcGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAgc                                                                                                             >  4:650602/1‑149 (MQ=255)
                                           gcgcAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGcgcg                                                                                                          >  2:7726/1‑149 (MQ=255)
                                              cAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGAtt                                                                                                       <  2:1031394/149‑1 (MQ=255)
                                                    attcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGa                                                                                                 <  3:331338/149‑1 (MQ=255)
                                                    attcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGa                                                                                                 <  3:767446/149‑1 (MQ=255)
                                                     ttcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGAt                                                                                                <  3:194403/149‑1 (MQ=255)
                                                     ttcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGAt                                                                                                <  4:603380/149‑1 (MQ=255)
                                                        acgacgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCa                                                                                             <  1:492028/149‑1 (MQ=255)
                                                         cgacgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCaa                                                                                            <  3:21583/149‑1 (MQ=255)
                                                         cgacgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCaa                                                                                            <  1:863946/149‑1 (MQ=255)
                                                          gacgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATaaa                                                                                                                                                   >  1:271888/1‑93 (MQ=255)
                                                          gacgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATaaa                                                                                                                                                   <  2:271888/93‑1 (MQ=255)
                                                          gacgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAAc                                                                                           <  2:874567/149‑1 (MQ=255)
                                                              gTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCtt                                                                                       <  4:331398/149‑1 (MQ=255)
                                                                ttattaATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTaa                                                                                     <  4:471466/149‑1 (MQ=255)
                                                                  attaattaAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATa                                                                                   <  4:1038471/149‑1 (MQ=255)
                                                                     aattaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGt                                                                                <  2:62128/149‑1 (MQ=255)
                                                                     aattaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGt                                                                                <  4:552438/149‑1 (MQ=255)
                                                                       ttaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGttt                                                                              >  2:1050992/1‑149 (MQ=255)
                                                                        taaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGtttt                                                                             <  2:591297/149‑1 (MQ=255)
                                                                           aaGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATc                                                                          >  1:168760/1‑149 (MQ=255)
                                                                             gCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCTATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCg                                                                        <  3:950033/149‑1 (MQ=255)
                                                                                aGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTc                                                                     <  4:183445/149‑1 (MQ=255)
                                                                                  aaaGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTa                                                                   <  3:14125/149‑1 (MQ=255)
                                                                                      ggTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACaaa                                                               <  1:553684/149‑1 (MQ=255)
                                                                                          tctcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGa                                                           <  3:425713/149‑1 (MQ=255)
                                                                                          tctcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGa                                                           <  4:48883/149‑1 (MQ=255)
                                                                                           ctcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAg                                                          <  1:7726/149‑1 (MQ=255)
                                                                                            tcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGc                                                         >  4:449608/1‑149 (MQ=255)
                                                                                            tcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGc                                                         >  3:781934/1‑149 (MQ=255)
                                                                                              aTTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCAt                                                       >  3:381399/1‑149 (MQ=255)
                                                                                              aTTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCAt                                                       >  1:494040/1‑149 (MQ=255)
                                                                                              aTTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCAt                                                       >  3:816915/1‑149 (MQ=255)
                                                                                               ttAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCAtt                                                      >  4:253356/1‑149 (MQ=255)
                                                                                               ttAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCAtt                                                      >  1:840610/1‑149 (MQ=255)
                                                                                                 aaaaaCAGAAAGCAGTTGTCAGGTCTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTAc                                                    <  3:149150/149‑1 (MQ=255)
                                                                                                 aaaaaCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTAc                                                    <  3:253356/149‑1 (MQ=255)
                                                                                                 aaaaaCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTAc                                                    <  3:868255/149‑1 (MQ=255)
                                                                                                 aaaaaCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTAc                                                    <  4:798264/149‑1 (MQ=255)
                                                                                                  aaaaCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACg                                                   >  1:606260/1‑149 (MQ=255)
                                                                                                    aaCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGcc                                                 >  3:995280/1‑149 (MQ=255)
                                                                                                       aGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGa                                              >  3:437083/1‑149 (MQ=255)
                                                                                                       aGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGa                                              <  3:368711/149‑1 (MQ=255)
                                                                                                        gAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGtt                                                                               <  1:301486/115‑1 (MQ=255)
                                                                                                        gAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGtt                                                                               >  2:301486/1‑115 (MQ=255)
                                                                                                        gAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGAc                                             <  4:997287/149‑1 (MQ=255)
                                                                                                         aaaGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGAcc                                            >  2:435999/1‑149 (MQ=255)
                                                                                                         aaaGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGAcc                                            >  4:584689/1‑149 (MQ=255)
                                                                                                            gCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGa                                                           <  2:477471/131‑1 (MQ=255)
                                                                                                            gCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGa                                                           >  1:477471/1‑131 (MQ=255)
                                                                                                               gTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATaaa                                      <  4:338839/149‑1 (MQ=255)
                                                                                                               gTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATaaa                                      <  2:65006/149‑1 (MQ=255)
                                                                                                                  gTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGaa                                   <  1:336707/149‑1 (MQ=255)
                                                                                                                   tCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAAc                                  <  4:90925/149‑1 (MQ=255)
                                                                                                                     aGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAAccc                                >  3:70448/1‑149 (MQ=255)
                                                                                                                               cTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTc                      <  4:819789/149‑1 (MQ=255)
                                                                                                                                         tcccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACtcatca            >  2:98138/2‑149 (MQ=255)
                                                                                                                                           ccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAgg          >  3:321462/1‑149 (MQ=255)
                                                                                                                                            cGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGc                                                         >  2:560647/1‑101 (MQ=255)
                                                                                                                                            cGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGt         <  4:70448/149‑1 (MQ=255)
                                                                                                                                            cGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGt         <  3:104026/149‑1 (MQ=255)
                                                                                                                                            cGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGt         >  4:972498/1‑149 (MQ=255)
                                                                                                                                              gagaATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGAtt                                                                                                       <  1:519826/53‑1 (MQ=255)
                                                                                                                                              gagaATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGAtt                                                                                                       >  2:519826/1‑53 (MQ=255)
                                                                                                                                                  aTAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGaaa   <  3:100264/149‑1 (MQ=255)
                                                                                                                                                   tAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGAAAg  >  2:470333/1‑149 (MQ=255)
                                                                                                                                                   tAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCAACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTAGCACTCATCAGGTTGGCAAg  >  4:117326/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGAGTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGAAAG  >  NC_000913/2255843‑2256138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: