Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,279,711 A→T 5.5% L17Q (CTG→CAG)  bcr ← bicyclomycin/cysteine/sulfonamide efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,279,7110AT 5.5% 426.0 / 10.7 125L17Q (CTG→CAG) bcrbicyclomycin/cysteine/sulfonamide efflux transporter
Reads supporting (aligned to +/- strand):  ref base A (57/61);  new base T (5/2);  total (62/63)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 135 total aligned reads displayed.

CCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAACGGACGATATTCACCGGGGGCTAAATCAGCATCCAGC  >  NC_000913/2279564‑2279857
                                                                                                                                                   |                                                                                                                                                  
ccGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTTCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCcagcag                                                                                                                                                   >  2:1005229/1‑149 (MQ=255)
ccGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTGCGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCcagcag                                                                                                                                                   <  2:595803/149‑1 (MQ=255)
ccGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCcagcag                                                                                                                                                   >  3:967745/1‑149 (MQ=255)
ccGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCcagcag                                                                                                                                                   >  1:892721/1‑149 (MQ=255)
  gTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGc                                                                                                                                                 >  4:66303/1‑149 (MQ=255)
       ttAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAgg                                                                                                                                            >  1:707864/1‑149 (MQ=255)
       ttAACTGCCCCAACGCAAAGCCCAGAATAGAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAgg                                                                                                                                            <  3:752610/149‑1 (MQ=255)
        tAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCTGCTGGCCatgtt                                                                                                                                           <  4:49561/149‑5 (MQ=255)
          aCTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATa                                                                                                                                         >  4:418009/1‑149 (MQ=255)
          aCTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATa                                                                                                                                         >  2:907522/1‑149 (MQ=255)
               cccAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCTGCTGGCCatgtt                                                                                                                                           <  1:1020517/142‑5 (MQ=255)
                ccAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACa                                                                                                                                   >  4:266002/1‑149 (MQ=255)
                 cAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACaa                                                                                                                                  <  2:973517/149‑1 (MQ=255)
                 cAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACaa                                                                                                                                  <  3:869368/149‑1 (MQ=255)
                  aaCGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAAt                                                                                                                                 <  1:400403/149‑1 (MQ=255)
                  aaCGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAAt                                                                                                                                 >  1:593710/1‑149 (MQ=255)
                   aCGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATa                                                                                                                                <  2:707864/149‑1 (MQ=255)
                     gCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGc                                                                                                                              <  3:112136/149‑1 (MQ=255)
                     gCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGc                                                                                                                              >  4:766857/1‑149 (MQ=255)
                        aaGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGTCCAAGGATAAAAACAATAGCaaa                                                                                                                           <  2:747912/149‑1 (MQ=255)
                               gAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGaa                                                                                                                    <  2:775785/149‑1 (MQ=255)
                               gAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGaa                                                                                                                    <  3:588082/149‑1 (MQ=255)
                               gAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGaa                                                                                                                    <  2:212946/149‑1 (MQ=255)
                                   ataAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGGTAAAAACAATAGCAAACGACGAATGCt                                                                                                                >  3:723930/1‑149 (MQ=255)
                                    taAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAg                                                                                                                                             >  2:95619/1‑119 (MQ=255)
                                    taAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAg                                                                                                                                             <  1:95619/119‑1 (MQ=255)
                                     aaGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGt                                                                                                              <  4:609523/149‑1 (MQ=255)
                                      aGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTc                                                                                                             <  2:12663/149‑1 (MQ=255)
                                       gTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCg                                                                                                            <  1:1483/149‑1 (MQ=255)
                                           tGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGggtg                                                                                                        >  4:780315/1‑149 (MQ=255)
                                           tGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGggtg                                                                                                        >  1:369872/1‑149 (MQ=255)
                                             aGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGggtggt                                                                                                      <  4:260685/149‑1 (MQ=255)
                                              gggTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAAt                                                                                                                   <  1:286793/135‑1 (MQ=255)
                                              gggTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTc                                                                                                     <  2:1043343/149‑1 (MQ=255)
                                              gggTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTc                                                                                                     <  3:62193/149‑1 (MQ=255)
                                              gggTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTc                                                                                                     <  2:93859/149‑1 (MQ=255)
                                               ggTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAacgac                                                                                                                       <  3:944302/130‑1 (MQ=255)
                                                 tCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTcaca                                                                                                  >  2:318498/1‑149 (MQ=255)
                                                      tGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACggg                                                                                             <  3:186219/149‑1 (MQ=255)
                                                                 cgccgGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGt                                                                                  >  3:456609/1‑149 (MQ=255)
                                                                    cgGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCAt                                                                               <  4:643729/149‑1 (MQ=255)
                                                                     ggTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCAtt                                                                              <  3:76999/149‑1 (MQ=255)
                                                                      gTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCgg                                                                                                           >  4:636344/1‑119 (MQ=255)
                                                                          gCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTc                                                                                                     >  4:552831/1‑121 (MQ=255)
                                                                          gCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTc                                                                                                     <  3:552831/121‑1 (MQ=255)
                                                                          gCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGaaaa                                                                         <  3:436820/149‑1 (MQ=255)
                                                                          gCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGaaaa                                                                         <  3:894359/149‑1 (MQ=255)
                                                                          gCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGaaaa                                                                         <  1:318498/149‑1 (MQ=255)
                                                                          gCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGaaaa                                                                         >  3:1029893/1‑149 (MQ=255)
                                                                            cAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGCAAGTCATTGAAAAGt                                                                       >  4:669780/1‑149 (MQ=255)
                                                                              aaCTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTa                                                                     <  3:780315/149‑1 (MQ=255)
                                                                                cTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAga                                                                   <  3:266002/149‑1 (MQ=255)
                                                                                cTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAga                                                                   <  4:456609/149‑1 (MQ=255)
                                                                                      tGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGc                                                             >  4:898804/1‑149 (MQ=255)
                                                                                      tGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGc                                                             >  1:677380/1‑149 (MQ=255)
                                                                                      tGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGc                                                             >  1:62463/1‑149 (MQ=255)
                                                                                      tGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGc                                                             >  2:817857/1‑149 (MQ=255)
                                                                                          aTTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGc                                                         >  2:876911/1‑149 (MQ=255)
                                                                                                  tAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATttctt                                                 <  2:593710/149‑1 (MQ=255)
                                                                                                   aGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATttcttc                                                <  2:892721/149‑1 (MQ=255)
                                                                                                     cgcgGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATttcttctt                                              <  3:898804/149‑1 (MQ=255)
                                                                                                      gcgGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATttcttcttc                                             >  4:182987/1‑149 (MQ=255)
                                                                                                       cgGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCa                                            <  1:789136/149‑1 (MQ=255)
                                                                                                         ggCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGt                                          <  1:1005229/149‑1 (MQ=255)
                                                                                                            aGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCa                                            <  3:140085/144‑1 (MQ=255)
                                                                                                            aGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCa                                            >  4:140085/1‑144 (MQ=255)
                                                                                                            aGATACATATCAATCGACAGCGACATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTaa                                       >  1:876729/1‑149 (MQ=255)
                                                                                                              aTACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTa                                                                     >  2:293237/1‑117 (MQ=255)
                                                                                                              aTACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATttctt                                                 <  2:215125/137‑1 (MQ=255)
                                                                                                              aTACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATttctt                                                 >  1:215125/1‑137 (MQ=255)
                                                                                                               tACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAAt                                                      <  1:1007522/131‑1 (MQ=255)
                                                                                                               tACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAAt                                                      >  2:1007522/1‑131 (MQ=255)
                                                                                                                  atatCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCa                                            <  2:259088/138‑1 (MQ=255)
                                                                                                                  atatCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCa                                            >  1:259088/1‑138 (MQ=255)
                                                                                                                     tCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATttcttctt                                              >  2:444200/1‑133 (MQ=255)
                                                                                                                     tCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATttcttctt                                              <  1:444200/133‑1 (MQ=255)
                                                                                                                     tCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAAc                                      <  2:11550/141‑1 (MQ=255)
                                                                                                                     tCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAAc                                      >  1:11550/1‑141 (MQ=255)
                                                                                                                       aaTCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAACGGACGATAtt                            >  2:86435/1‑149 (MQ=255)
                                                                                                                       aaTCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAACGGACGATAtt                            >  1:51342/1‑149 (MQ=255)
                                                                                                                          cGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGACTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAACGGACGATATTCAc                         <  2:422175/149‑1 (MQ=255)
                                                                                                                           gACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGt                                          <  1:878163/131‑1 (MQ=255)
                                                                                                                            aCAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAAc                                      <  3:51674/134‑1 (MQ=255)
                                                                                                                            aCAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAAc                                      >  4:51674/1‑134 (MQ=255)
                                                                                                                            aCAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAACGGACGATATTCACCg                       <  4:122614/149‑1 (MQ=255)
                                                                                                                               gCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAACGGACGATATTCACCgggg                    >  1:294361/1‑149 (MQ=255)
                                                                                                                                       aaCATCGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAACGGACGATATTCACCGGGGGCTAAATc            <  4:449812/149‑1 (MQ=255)
                                                                                                                                        aCATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGtt                                         >  2:302266/1‑119 (MQ=255)
                                                                                                                                        aCATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGtt                                         <  1:302266/119‑1 (MQ=255)
                                                                                                                                        aCATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAACGGACGATATTCATCGGGGGCTAAATCa           <  3:75915/149‑1 (MQ=255)
                                                                                                                                         cttGGCCTGCTGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAACGGACGATATTCACCGGGGGCTAAATCAg          >  2:442424/3‑149 (MQ=255)
                                                                                                                                         cATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAACGGACGATATTCACCGGGGGCTAAATCAg          <  2:369872/149‑1 (MQ=255)
                                                                                                                                          aTGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAACGGACGATATTCACCGGGGGCTAAATCAGc         >  3:319593/1‑149 (MQ=255)
                                                                                                                                          aTGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAACGGACGATATTCACCGGGGGCTAAATCAGc         >  1:84117/1‑149 (MQ=255)
                                                                                                                                          aTGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAACGGACGATATTCACCGGGGGCTAAATCAGc         <  1:25874/149‑1 (MQ=255)
                                                                                                                                           tGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGGGGTCAGAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGACAATTTCTTCTTCAGTTAACGGACGATATTCACCGGGGCCTAAATCAGCa        >  2:365814/1‑149 (MQ=255)
                                                                                                                                            ggCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAACGGACGATATTCACCGGGGGCTAAATCAGCAt       >  4:938882/1‑149 (MQ=255)
                                                                                                                                               cagcagGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAACGGACGATATTCACCGGGGGCTAAATCAGCATCCa    <  4:1029893/149‑1 (MQ=255)
                                                                                                                                                agcagGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAACGGACGATATTCACCGGGGGCTAAATCAGCATCCAg   >  2:662649/1‑149 (MQ=255)
                                                                                                                                                agcagGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAACGGACGATATTCACCGGGGGCTAAATCAGCATCCAg   >  2:1008608/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGGCTCCTGAAAGTCATTGAAAAGTTAGACGACGCTGGCAATTTCTTCTTCAGTTAACGGACGATATTCACCGGGGGCTAAATCAGCATCCAGC  >  NC_000913/2279564‑2279857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: