Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,493,563 T→A 5.1% intergenic (‑309/+204) frc ← / ← yfdX formyl‑CoA transferase, NAD(P)‑binding/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,493,5630TA 5.1% 476.5 / 12.3 137intergenic (‑309/+204)frc/yfdXformyl‑CoA transferase, NAD(P)‑binding/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (61/69);  new base A (2/5);  total (63/74)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01

Only 100 of 147 total aligned reads displayed.

TTTCTGCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCAT  >  NC_000913/2493415‑2493708
                                                                                                                                                    |                                                                                                                                                 
tttCTGCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCttttt                                                                                                                                                   >  4:949074/1‑149 (MQ=255)
tttCTGCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCttttt                                                                                                                                                   >  3:1001565/1‑149 (MQ=255)
tttCTGCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACAGTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCttttt                                                                                                                                                   >  3:309389/1‑149 (MQ=255)
   cTGCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTaaa                                                                                                                                                <  1:1024549/149‑1 (MQ=255)
   cTGCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTaaa                                                                                                                                                <  3:665922/149‑1 (MQ=255)
   cTGCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTaaa                                                                                                                                                <  1:971982/149‑1 (MQ=255)
   cTGCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAg                                                                                                                                               <  3:403876/149‑1 (MQ=255)
        tGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTa                                                                                                                                           >  4:330404/1‑149 (MQ=255)
         ggTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTAc                                                                                                                                          <  3:993879/149‑1 (MQ=255)
         ggTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTAAAAAGCGTaa                                                                                                                                          <  1:238423/149‑2 (MQ=255)
               tagtGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGAGTACGCTTTAAAAAGCGTaatcatac                                                                                                                                    <  4:544469/149‑8 (MQ=255)
                 gtGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAaac                                                                                                                                  >  4:690216/1‑149 (MQ=255)
                  tGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAaaca                                                                                                                                 >  2:344647/1‑149 (MQ=255)
                  tGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAaaca                                                                                                                                 >  1:1012754/1‑149 (MQ=255)
                     ggACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAaacataa                                                                                                                              <  1:1038226/149‑1 (MQ=255)
                     ggACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAaacataa                                                                                                                              <  2:17579/149‑1 (MQ=255)
                     ggACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAaacataa                                                                                                                              <  3:949074/149‑1 (MQ=255)
                     ggACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAaacataa                                                                                                                              <  4:890428/149‑1 (MQ=255)
                       aCGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAaacataaca                                                                                                                            >  2:125443/1‑149 (MQ=255)
                         gAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTAAAAAGCGTaat                                                                                                                                         >  4:727631/1‑132 (MQ=255)
                         gAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTAAAAAGCGTaat                                                                                                                                         <  3:727631/134‑3 (MQ=255)
                           aaaGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACataat                                                                                                                        >  4:593385/1‑149 (MQ=255)
                           aaaGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACataat                                                                                                                        <  1:644720/149‑1 (MQ=255)
                            aagaagAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGAGTACCATAAAACAAAACataata                                                                                                                       >  4:242345/1‑149 (MQ=255)
                              gaagaaTATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGATATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATaaa                                                                                                                     <  4:901286/149‑1 (MQ=255)
                                 gaaTATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGa                                                                                                                  <  3:859138/149‑1 (MQ=255)
                                  aaTATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGAt                                                                                                                 <  2:704659/149‑1 (MQ=255)
                                  aaTATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGAt                                                                                                                 >  4:518681/1‑149 (MQ=255)
                                      tGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTtgt                                                                                                             <  2:204424/149‑1 (MQ=255)
                                      tGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTtgt                                                                                                             <  3:61801/149‑1 (MQ=255)
                                       gATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTtgtg                                                                                                            >  1:975561/1‑149 (MQ=255)
                                        aTAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGtga                                                                                                           >  1:133874/1‑149 (MQ=255)
                                        aTAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGGGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGtga                                                                                                           >  2:1022161/1‑149 (MQ=255)
                                         tAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGtgat                                                                                                          <  2:975561/149‑1 (MQ=255)
                                                 gATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACggg                                                                                                  <  1:41116/149‑1 (MQ=255)
                                                  aTTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGa                                                                                                 <  2:730559/149‑1 (MQ=255)
                                                  aTTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGa                                                                                                 <  2:53222/149‑1 (MQ=255)
                                                  aTTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGa                                                                                                 >  2:277441/1‑149 (MQ=255)
                                                       tGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGattatt                                                                                            >  2:986012/1‑149 (MQ=255)
                                                         gTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGc                                                                                          >  4:333303/1‑149 (MQ=255)
                                                          tAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCg                                                                                         <  4:696101/149‑1 (MQ=255)
                                                          tAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCg                                                                                         <  1:858477/149‑1 (MQ=255)
                                                                gagagagTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTAc                                                                                   <  1:815627/149‑1 (MQ=255)
                                                                gagagagTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTAc                                                                                   <  3:281139/149‑1 (MQ=255)
                                                                   agagTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACttt                                                                                <  4:644614/149‑1 (MQ=255)
                                                                     agTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGa                                                                              <  1:586331/149‑1 (MQ=255)
                                                                      gTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGa                                                                              <  2:848791/148‑1 (MQ=255)
                                                                      gTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAg                                                                             <  1:69096/149‑1 (MQ=255)
                                                                      gTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAg                                                                             <  2:1012754/149‑1 (MQ=255)
                                                                      gTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAg                                                                             <  3:330404/149‑1 (MQ=255)
                                                                         gcgACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAaacataa                                                                                                                              <  3:888033/97‑1 (MQ=255)
                                                                         gcgACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGaa                                                                          <  1:590701/149‑1 (MQ=255)
                                                                                   tgATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGc                                                                >  4:1005069/1‑149 (MQ=255)
                                                                                   tgATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGc                                                                <  3:211856/149‑1 (MQ=255)
                                                                                    gATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCg                                                               >  2:86124/1‑149 (MQ=255)
                                                                                     ttGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGCGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGa                                                              >  2:904439/2‑149 (MQ=255)
                                                                                     aTGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGa                                                              <  1:332509/149‑1 (MQ=255)
                                                                                     aTGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGa                                                              <  4:393944/149‑1 (MQ=255)
                                                                                       gTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGacac                                                                                                     <  4:261947/108‑1 (MQ=255)
                                                                                       gTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGacac                                                                                                     >  3:261947/1‑108 (MQ=255)
                                                                                       gTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGttt                                                                                     <  1:262435/124‑1 (MQ=255)
                                                                                       gTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGttt                                                                                     >  2:262435/1‑124 (MQ=255)
                                                                                       gTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATaaaa                                                                   >  2:474191/1‑142 (MQ=255)
                                                                                       gTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATaaaa                                                                   <  1:474191/142‑1 (MQ=255)
                                                                                       gTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGt                                                            >  1:332996/1‑149 (MQ=255)
                                                                                        ttATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGt                                                                                       <  3:784588/121‑1 (MQ=255)
                                                                                        ttATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGt                                                                                       >  4:784588/1‑121 (MQ=255)
                                                                                          aTTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTta                                                         >  4:380369/1‑149 (MQ=255)
                                                                                           ttCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTtat                                                        >  2:48278/1‑149 (MQ=255)
                                                                                           ttCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTtat                                                        >  3:429020/1‑149 (MQ=255)
                                                                                           ttCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGAATTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTtat                                                        >  2:613345/1‑149 (MQ=255)
                                                                                              ttACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAg                                                      >  1:937869/1‑148 (MQ=255)
                                                                                                    cTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTtat                                                        <  4:801439/140‑1 (MQ=255)
                                                                                                     tGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGa                                              >  3:837247/1‑149 (MQ=255)
                                                                                                          gaagCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGcc                                         >  3:252043/1‑149 (MQ=255)
                                                                                                                 cGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAaataat                                  >  1:729725/1‑149 (MQ=255)
                                                                                                                  ggTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAAtt                                 <  1:717184/149‑1 (MQ=255)
                                                                                                                     gATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTa                              <  4:733521/149‑1 (MQ=255)
                                                                                                                      aTTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTAt                             <  3:1005069/149‑1 (MQ=255)
                                                                                                                      aTTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTAt                             <  3:642010/149‑1 (MQ=255)
                                                                                                                             agcaagGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACcaca                      >  3:558310/1‑149 (MQ=255)
                                                                                                                               caagGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAAc                    <  1:754674/149‑1 (MQ=255)
                                                                                                                                  gggTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAg                                                      >  3:656218/1‑112 (MQ=255)
                                                                                                                                  gggTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAg                                                      <  4:656218/112‑1 (MQ=255)
                                                                                                                                  gggTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGATTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTg                 >  2:5082/1‑149 (MQ=255)
                                                                                                                                    gTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTc               >  3:254986/1‑149 (MQ=255)
                                                                                                                                    gTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTc               <  2:62900/149‑1 (MQ=255)
                                                                                                                                    gTATGATTACGCCTTTTAAAGCGTACCATAAAACAAAACATAATAAATGAGTTGTGATTAAACGTGATTATTGCGGTTTAGATTGAGGAATATAAAATGCGAGTTTATAATACCACGATCGCTAAATATTTTTATAACCACAACCTGTc               >  2:860574/1‑149 (MQ=255)
                                                                                                                                     tATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGc                                                                <  3:781451/99‑1 (MQ=255)
                                                                                                                                     tATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGc                                                                >  4:781451/1‑99 (MQ=255)
                                                                                                                                          ttACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATtg         >  1:993842/1‑149 (MQ=255)
                                                                                                                                           tACGCTTTAAAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGt             >  4:256634/1‑144 (MQ=255)
                                                                                                                                            aCGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATtgtg       <  1:125443/149‑1 (MQ=255)
                                                                                                                                             cGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTgg      <  4:985626/149‑1 (MQ=255)
                                                                                                                                              gCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGt     >  4:777389/1‑149 (MQ=255)
                                                                                                                                              gCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGt     >  2:196728/1‑149 (MQ=255)
                                                                                                                                                tttttAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAAc                    <  2:1049547/132‑1 (MQ=255)
                                                                                                                                                tttttAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCa   >  1:839465/1‑149 (MQ=255)
                                                                                                                                                tttttAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCa   >  4:225997/1‑149 (MQ=255)
                                                                                                                                                 ttttAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCAt  <  3:561096/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TTTCTGCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCAT  >  NC_000913/2493415‑2493708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: