Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,535,981 G→A 100% G50S (GGT→AGT)  crr → glucose‑specific enzyme IIA component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,535,9810GA100.0% 432.7 / NA 120G50S (GGT→AGT) crrglucose‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (60/60);  total (60/60)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 120 total aligned reads displayed.

GTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTG  >  NC_000913/2535838‑2536125
                                                                                                                                               |                                                                                                                                                
gTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTAtt                                                                                                                                             <  3:378592/149‑1 (MQ=255)
   tGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCt                                                                                                                                          <  3:934538/149‑1 (MQ=255)
     ttCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTAt                                                                                                                                        >  3:698863/1‑149 (MQ=255)
     ttCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTAt                                                                                                                                        >  1:969504/1‑149 (MQ=255)
     ttCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTAt                                                                                                                                        >  4:200932/1‑149 (MQ=255)
     atCGCTAAACTGAAATGTCTGGGTTCCGACGACAAGAAGGAGACTGGAACTATTGAGATCATTGCTCCGCTGTCTGGCGAGATCGGCAATATCGAAGACGTGCCGGATGTCGTTGTTGCGGAAAAAATCGTTGGTGATAGTATTGCTAt                                                                                                                                        >  4:893217/2‑149 (MQ=255)
             aCTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCaa                                                                                                                                <  2:144598/149‑1 (MQ=255)
             aCTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCaa                                                                                                                                <  4:573051/149‑1 (MQ=255)
              cTGAAATCTCTGGTTTCCGACGACAAGAAGTATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAAc                                                                                                                               <  3:851740/149‑1 (MQ=255)
                gAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACgg                                                                                                                             <  4:951988/149‑1 (MQ=255)
                gAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACgg                                                                                                                             <  2:456637/149‑1 (MQ=255)
                   aTCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTa                                                                                                                          <  4:833131/149‑1 (MQ=255)
                     ctctGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAAc                                                                                                                        >  2:332706/1‑149 (MQ=255)
                      tctGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACa                                                                                                                       >  1:896825/1‑149 (MQ=255)
                      tctGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACa                                                                                                                       >  4:661088/1‑149 (MQ=255)
                          gTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAAt                                                                                                                   <  3:492195/149‑1 (MQ=255)
                               cgacgaCAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTAtt                                                                                                                                             >  1:234061/1‑118 (MQ=255)
                                      aagaagGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGt                                                                                                       >  2:891770/1‑149 (MQ=255)
                                      aagaagGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGt                                                                                                       <  1:1014119/149‑1 (MQ=255)
                                         aagGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTAt                                                                                                                                              <  4:468239/107‑1 (MQ=255)
                                          agGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGAc                                                                                                   <  3:378900/149‑1 (MQ=255)
                                          agGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTAGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGAc                                                                                                   <  2:799546/149‑1 (MQ=255)
                                            gATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACgg                                                                                                                             <  4:415964/121‑1 (MQ=255)
                                            gATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACgg                                                                                                                             >  3:415964/1‑121 (MQ=255)
                                            gATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATAACGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACgg                                                                                                 >  1:439069/1‑149 (MQ=255)
                                                 cGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCATCGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCa                                                                                            <  2:306070/149‑1 (MQ=255)
                                                  ggAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCAt                                                                                           >  2:715262/1‑149 (MQ=255)
                                                    aaCTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTg                                                                                         <  1:608558/149‑1 (MQ=255)
                                                       tATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGCCACCATTGGTa                                                                                      <  2:99719/149‑1 (MQ=255)
                                                       tATCGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAATAAAATGGTCGCGCCAGTAGACGGCACCATTGGTa                                                                                      >  3:585268/1‑149 (MQ=255)
                                                        aTTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTaa                                                                                     >  1:475322/1‑149 (MQ=255)
                                                        aTTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTaa                                                                                     >  4:420031/1‑149 (MQ=255)
                                                         ttGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTaaa                                                                                    >  1:483050/1‑149 (MQ=255)
                                                              aTCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCtt                                                                               >  1:829264/1‑149 (MQ=255)
                                                               tCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCttt                                                                              <  3:969184/149‑1 (MQ=255)
                                                               tCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCttt                                                                              >  4:218539/1‑149 (MQ=255)
                                                               tCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCttt                                                                              >  3:164902/1‑149 (MQ=255)
                                                                 aTTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGa                                                                            >  1:1017813/1‑149 (MQ=255)
                                                                 aTTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGa                                                                            <  4:358166/149‑1 (MQ=255)
                                                                    gCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAaac                                                                         <  3:837316/149‑1 (MQ=255)
                                                                      tCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAAc                                                                                                                               <  4:801720/93‑1 (MQ=255)
                                                                      tCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAAc                                                                                                                               >  3:801720/1‑93 (MQ=255)
                                                                       ccGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAaaccaa                                                                      >  3:634600/1‑149 (MQ=255)
                                                                        cgcgCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCGACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAaaccaac                                                                     <  2:589285/149‑1 (MQ=255)
                                                                        cGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAaaccaac                                                                     >  3:532864/1‑149 (MQ=255)
                                                                        cGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAaaccaac                                                                     <  2:1055315/149‑1 (MQ=255)
                                                                           tctctGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCAc                                                                  >  1:1018564/1‑149 (MQ=255)
                                                                             tctGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGc                                                                <  3:234794/149‑1 (MQ=255)
                                                                               tGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCAt                                                              >  2:613933/1‑149 (MQ=255)
                                                                                    agaTCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTctct                                                         >  4:343444/1‑149 (MQ=255)
                                                                                     gaTCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTa                                                        <  1:542284/149‑1 (MQ=255)
                                                                                      aTCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTAt                                                       <  4:696047/149‑1 (MQ=255)
                                                                                      aTCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTAt                                                       <  3:350730/149‑1 (MQ=255)
                                                                                         gTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGa                                                    >  2:520766/1‑149 (MQ=255)
                                                                                         gTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGa                                                    >  4:497693/1‑149 (MQ=255)
                                                                                         gTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGa                                                    >  3:252175/1‑149 (MQ=255)
                                                                                          tCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCttt                                                                              >  1:1027082/1‑122 (MQ=255)
                                                                                          tCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCttt                                                                              <  2:1027082/122‑1 (MQ=255)
                                                                                          tCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGaa                                                   >  4:718805/1‑149 (MQ=255)
                                                                                          tCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGaa                                                   <  2:177783/149‑1 (MQ=255)
                                                                                          tCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGaa                                                   >  4:944507/1‑149 (MQ=255)
                                                                                            aaTATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTa                                                        >  3:541534/1‑142 (MQ=255)
                                                                                            aaTATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTAATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTa                                                        <  4:541534/142‑1 (MQ=255)
                                                                                                 cGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATa                                            <  4:747678/149‑1 (MQ=255)
                                                                                                  gAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAg                                           >  2:257524/1‑149 (MQ=255)
                                                                                                         tGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTc                                                                                                               >  1:439883/1‑74 (MQ=255)
                                                                                                         tGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTc                                                                                                               <  2:439883/74‑1 (MQ=255)
                                                                                                         tGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAg                                                                                                        >  4:795362/1‑81 (MQ=255)
                                                                                                         tGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAg                                                                                                        <  3:795362/81‑1 (MQ=255)
                                                                                                         tGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGtt                                    <  1:891770/149‑1 (MQ=255)
                                                                                                         tGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGtt                                    <  3:218539/149‑1 (MQ=255)
                                                                                                         tGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGtt                                    <  1:990630/149‑1 (MQ=255)
                                                                                                            cGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGaa                                 <  4:429536/149‑1 (MQ=255)
                                                                                                            cGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGaa                                 <  2:680052/149‑1 (MQ=255)
                                                                                                              gATGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACt                               <  4:252175/149‑1 (MQ=255)
                                                                                                                tGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGt                             <  1:960274/149‑1 (MQ=255)
                                                                                                                tGTCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGt                             <  3:718805/149‑1 (MQ=255)
                                                                                                                  tCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTc                           >  1:881485/1‑149 (MQ=255)
                                                                                                                  tCGTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTc                           >  1:110721/1‑149 (MQ=255)
                                                                                                                    gTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTaa                                                                                                                         <  2:531754/53‑1 (MQ=255)
                                                                                                                    gTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTaa                                                                                                                         >  1:531754/1‑53 (MQ=255)
                                                                                                                    gTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGt                         >  4:161151/1‑149 (MQ=255)
                                                                                                                    gTTTTTGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGt                         <  2:896825/149‑1 (MQ=255)
                                                                                                                     tttttGCGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTc                        >  3:130725/1‑149 (MQ=255)
                                                                                                                           cGGAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTc                  <  4:532864/149‑1 (MQ=255)
                                                                                                                            ggAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCg                          >  2:226676/1‑140 (MQ=255)
                                                                                                                            ggAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCg                 >  2:160674/1‑149 (MQ=255)
                                                                                                                             gAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGGCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTccg                >  2:213640/1‑147 (MQ=255)
                                                                                                                             gAAAAAATCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCgg                <  3:172781/149‑1 (MQ=255)
                                                                                                                               aaaaaTCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTa              >  3:313772/1‑149 (MQ=255)
                                                                                                                                aaaaTCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAATAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTAt             <  4:585268/149‑1 (MQ=255)
                                                                                                                                 aaaTCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAATATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGACCTGTTCGTCCACTTCGGTATc            <  2:952263/149‑1 (MQ=255)
                                                                                                                                   aTCGTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGa          <  2:705915/149‑1 (MQ=255)
                                                                                                                                      gTTGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGacac       >  1:540003/1‑149 (MQ=255)
                                                                                                                                       ttGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACAcc      >  2:775501/1‑149 (MQ=255)
                                                                                                                                       ttGGTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACAcc      >  1:783792/1‑149 (MQ=255)
                                                                                                                                         ggTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGt    >  4:1001667/1‑149 (MQ=255)
                                                                                                                                          gTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATc            >  2:503654/1‑140 (MQ=255)
                                                                                                                                          gTGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATc            <  1:503654/140‑1 (MQ=255)
                                                                                                                                           tGATAGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTg  <  3:795843/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTG  >  NC_000913/2535838‑2536125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: