Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,630,919 G→A 5.4% intergenic (+54/+39) yfgI → / ← guaA nalidixic acid resistance protein, putative periplasmic protein/GMP synthetase (glutamine aminotransferase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,630,9190GA 5.4% 428.2 / 7.5 128intergenic (+54/+39)yfgI/guaAnalidixic acid resistance protein, putative periplasmic protein/GMP synthetase (glutamine aminotransferase)
Reads supporting (aligned to +/- strand):  ref base G (61/60);  new base A (2/5);  total (63/65)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 133 total aligned reads displayed.

GCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATC  >  NC_000913/2630776‑2631065
                                                                                                                                               |                                                                                                                                                  
gCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACa                                                                                                                                               <  4:744098/149‑1 (MQ=255)
   aaCAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACagag                                                                                                                                            <  4:104014/149‑1 (MQ=255)
   aaCAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACagag                                                                                                                                            >  2:577430/1‑149 (MQ=255)
   aaCAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACagag                                                                                                                                            >  1:286946/1‑149 (MQ=255)
   aaCAGAAATATGACTGAAGGCGGTAGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACagag                                                                                                                                            >  3:265864/1‑149 (MQ=255)
    aCAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAgg                                                                                                                                           >  2:181137/1‑149 (MQ=255)
    aCAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCAGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAgg                                                                                                                                           <  1:497382/149‑1 (MQ=255)
     cAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTTACTACAGAggg                                                                                                                                          <  1:743944/149‑1 (MQ=255)
       gAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGtt                                                                                                                                        >  4:871900/1‑149 (MQ=255)
          atatGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTg                                                                                                                                     <  3:650660/149‑1 (MQ=255)
             tGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttc                                                                                                                                  <  4:115399/149‑1 (MQ=255)
               aCTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttcat                                                                                                                                <  1:591171/149‑1 (MQ=255)
                cTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttcatt                                                                                                                               <  4:610416/149‑1 (MQ=255)
                 tGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTTACTACAGAggg                                                                                                                                          <  1:343809/137‑1 (MQ=255)
                 tGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGtttt                                                                                                                                      <  3:557729/141‑1 (MQ=255)
                 tGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGtttt                                                                                                                                      >  4:557729/1‑141 (MQ=255)
                          tCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAg                                                                                                                     <  1:181137/149‑1 (MQ=255)
                              gATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAGTTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTc                                                                                                                 >  2:914229/1‑149 (MQ=255)
                              gATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTc                                                                                                                 >  4:976985/1‑149 (MQ=255)
                              gATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTc                                                                                                                 >  1:413652/1‑149 (MQ=255)
                                 cTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCaaa                                                                                                              <  3:367164/149‑1 (MQ=255)
                                  tgatgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTTACTACAGAggg                                                                                                                                          >  3:574680/1‑120 (MQ=255)
                                  tgatgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAgg                                                                                                                                           >  2:526692/1‑119 (MQ=255)
                                  tgatgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAAt                                                                                                             >  3:96484/1‑149 (MQ=255)
                                    atgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCa                                                                                                           <  1:6778/149‑1 (MQ=255)
                                    atgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCa                                                                                                           >  1:146084/1‑149 (MQ=255)
                                      gaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCAtt                                                                                                         <  3:412908/149‑1 (MQ=255)
                                      gaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCAtt                                                                                                         >  3:41843/1‑149 (MQ=255)
                                         aaGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTccc                                                                                                      <  3:871900/149‑1 (MQ=255)
                                         aaGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTccc                                                                                                      <  3:761534/149‑1 (MQ=255)
                                           gCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCAc                                                                                                    <  4:724752/149‑1 (MQ=255)
                                             gTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGtttt                                                                                                                                      <  2:413738/113‑1 (MQ=255)
                                                ggCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAAt                                                                                               <  1:577430/149‑1 (MQ=255)
                                                ggCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAAt                                                                                               <  2:273474/149‑1 (MQ=255)
                                                     aGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttc                                                                                                                                  >  2:285901/1‑109 (MQ=255)
                                                     aGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttc                                                                                                                                  <  1:285901/109‑1 (MQ=255)
                                                              gaACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCt                                                                                 >  2:49827/1‑149 (MQ=255)
                                                              gaACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCt                                                                                 <  3:251614/149‑1 (MQ=255)
                                                               aaCAAATCGGACAGCTGGTTTAATTAATCGCTGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTAGAGTAACAGAGGGTTTAGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGTGGCtt                                                                                >  2:164135/1‑149 (MQ=255)
                                                               aaCAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttcat                                                                                                                                >  2:1014617/1‑101 (MQ=255)
                                                               aaCAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttcat                                                                                                                                <  1:1014617/101‑1 (MQ=255)
                                                                aCAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCGACTCAATGGTAGCTGGCGGCTTg                                                                               >  4:731008/1‑149 (MQ=255)
                                                                 cAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTTACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGc                                                                              <  3:731008/149‑1 (MQ=255)
                                                                       gtaCAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGTTAAAAAACCCTCTGTAGTAACAGAGAGCGGTGTTCAGTCATACTGCAGCGTCTAATCATGCCCACTCACTGGTAGCTGGCGGCTTGCCggtta                                                                        <  2:700530/147‑5 (MQ=255)
                                                                       ggACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGa                                                                        <  2:413652/149‑1 (MQ=255)
                                                                        gACATCTAGTTTAATTAATCGATGTTAGTAACTTCAATGCTGTAATTCTTGAAGATAAAAAACCCTCTGAAGTAACAGCGGGTTATCTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTCGCTTGTGGCTTGCCGCTGAt                                                                       <  2:763804/149‑1 (MQ=255)
                                                                        gACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGAt                                                                       <  2:639764/149‑1 (MQ=255)
                                                                        gACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCCGTAGTAACAGAGGGTTTTTATCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCCCAGAt                                                                       <  4:893893/149‑1 (MQ=255)
                                                                            gCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTTAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAACTCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCa                                                                   <  4:41843/149‑1 (MQ=255)
                                                                            gCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCa                                                                   <  1:77320/149‑1 (MQ=255)
                                                                            gCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCa                                                                   <  4:670725/149‑1 (MQ=255)
                                                                                     attaatCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATa                                                                 >  4:791817/1‑142 (MQ=255)
                                                                                         atCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCGATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCggg                                                      <  4:622659/149‑1 (MQ=255)
                                                                                         atCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCggg                                                      >  1:1010224/1‑149 (MQ=255)
                                                                                          tCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGTGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGa                                                     >  3:18394/1‑149 (MQ=255)
                                                                                          tCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGa                                                     <  1:1036776/149‑1 (MQ=255)
                                                                                            gATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATa                                                                 <  3:1008678/135‑1 (MQ=255)
                                                                                            gATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATa                                                                 >  4:1008678/1‑135 (MQ=255)
                                                                                               gTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttc                                                                                                                                  <  4:818001/67‑1 (MQ=255)
                                                                                               gTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttc                                                                                                                                  >  3:818001/1‑67 (MQ=255)
                                                                                                  aGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCa                                                                                                     <  2:920504/93‑1 (MQ=255)
                                                                                                  aGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCa                                                                                                     >  1:920504/1‑93 (MQ=255)
                                                                                                  aGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGt                                             <  1:1005527/149‑1 (MQ=255)
                                                                                                   gTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATCGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGtt                                            >  1:580653/1‑149 (MQ=255)
                                                                                                   gTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGtt                                            <  2:492342/149‑1 (MQ=255)
                                                                                                    tAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTc                                           >  1:1020386/1‑149 (MQ=255)
                                                                                                    tAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTc                                           >  3:992795/1‑149 (MQ=255)
                                                                                                        ttCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACtt                                       >  1:410808/1‑149 (MQ=255)
                                                                                                        ttCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACtt                                       <  4:96484/149‑1 (MQ=255)
                                                                                                        ttCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACtt                                       >  1:600482/1‑149 (MQ=255)
                                                                                                        ttCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACtt                                       >  4:281630/1‑149 (MQ=255)
                                                                                                        ttCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACtt                                       >  2:223860/1‑149 (MQ=255)
                                                                                                        ttCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACtt                                       >  1:695207/1‑149 (MQ=255)
                                                                                                           aaTCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCAt                                    >  1:491487/1‑149 (MQ=255)
                                                                                                            aTCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCAtt                                   <  2:702135/149‑1 (MQ=255)
                                                                                                                 ataATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCAtt                                   <  1:301147/144‑1 (MQ=255)
                                                                                                                 ataATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCAtt                                   >  2:301147/1‑144 (MQ=255)
                                                                                                                 ataATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATaa                              <  3:230833/149‑1 (MQ=255)
                                                                                                                       cTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGt                        <  3:103558/149‑1 (MQ=255)
                                                                                                                        ttGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTg                                                                                       <  3:562734/85‑1 (MQ=255)
                                                                                                                        ttGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTg                                                                                       >  4:562734/1‑85 (MQ=255)
                                                                                                                        ttGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGtt                       >  1:655875/1‑149 (MQ=255)
                                                                                                                        ttGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGtt                       <  1:1052477/149‑1 (MQ=255)
                                                                                                                        ttGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGtt                       >  1:471177/1‑149 (MQ=255)
                                                                                                                        ttGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGtt                       <  2:580653/149‑1 (MQ=255)
                                                                                                                             gATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGaaa                  <  2:372448/149‑1 (MQ=255)
                                                                                                                              aTAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAAc                 <  2:368907/149‑1 (MQ=255)
                                                                                                                                 aaaaaCCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAAcgcg              >  2:906368/1‑149 (MQ=255)
                                                                                                                                 aaaaaCCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAAcgcg              >  4:464444/1‑149 (MQ=255)
                                                                                                                                  aaaaCCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGa             >  3:829639/1‑149 (MQ=255)
                                                                                                                                   aaaCCCTCTGTTACTACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCAtt                                   <  3:177660/126‑1 (MQ=255)
                                                                                                                                   aaaCCCTCTGTTACTACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCAtt                                   >  4:177660/1‑126 (MQ=255)
                                                                                                                                   aaaCCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGAc            >  3:101340/1‑149 (MQ=255)
                                                                                                                                       ccTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAg        >  1:1040711/1‑149 (MQ=255)
                                                                                                                                        ctctGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAgg       >  4:502634/1‑149 (MQ=255)
                                                                                                                                         tctGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGa      >  2:181999/1‑149 (MQ=255)
                                                                                                                                             tagtaACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATc  <  1:181999/149‑1 (MQ=255)
                                                                                                                                             tagtaACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATc  >  3:333311/1‑149 (MQ=255)
                                                                                                                                             tagtaACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATc  <  2:410808/149‑1 (MQ=255)
                                                                                                                                               gtaACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATc  >  2:1033430/1‑147 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
GCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATC  >  NC_000913/2630776‑2631065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: