Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,753,986 A→T 9.7% intergenic (+40/+22) bamE → / ← ratB lipoprotein component of BamABCDE OM biogenesis complex/UPF0125 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,753,9860AT 9.7% 466.2 / 33.1 144intergenic (+40/+22)bamE/ratBlipoprotein component of BamABCDE OM biogenesis complex/UPF0125 family protein
Reads supporting (aligned to +/- strand):  ref base A (71/59);  new base T (7/7);  total (78/66)
Fisher's exact test for biased strand distribution p-value = 7.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

Only 100 of 145 total aligned reads displayed.

CCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGT  >  NC_000913/2753838‑2754132
                                                                                                                                                    |                                                                                                                                                   
   gCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGa                                                                                                                                                  <  3:742701/149‑1 (MQ=255)
   gCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGa                                                                                                                                                  <  2:131475/149‑1 (MQ=255)
     aaCCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGGCCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGAGCTCAATGAGc                                                                                                                                                >  3:685697/1‑149 (MQ=255)
      aCCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCa                                                                                                                                               >  2:988870/1‑149 (MQ=255)
       ccAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCAc                                                                                                                                              >  1:76482/1‑149 (MQ=255)
           gTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCttt                                                                                                                                          <  3:59264/149‑1 (MQ=255)
           gTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCttt                                                                                                                                          <  1:103207/149‑1 (MQ=255)
            tCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCtttt                                                                                                                                         <  2:600659/149‑1 (MQ=255)
                gAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtct                                                                                                                                    >  2:177186/1‑149 (MQ=255)
                gAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGATGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtct                                                                                                                                    >  4:953507/1‑149 (MQ=255)
                   ggTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctgtc                                                                                                                                 <  2:100401/149‑1 (MQ=255)
                     tgtAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctgtctg                                                                                                                               >  2:941283/1‑149 (MQ=255)
                      gtAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTcagtctgt                                                                                                                              >  2:443771/1‑149 (MQ=255)
                          cTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGttatt                                                                                                                          <  2:600583/149‑1 (MQ=255)
                           tCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGttattt                                                                                                                         >  4:859572/1‑149 (MQ=255)
                           tCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGttattt                                                                                                                         >  4:550552/1‑149 (MQ=255)
                             aGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGttatttat                                                                                                                       >  4:441636/1‑149 (MQ=255)
                             aGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGttatttat                                                                                                                       <  1:1046655/149‑1 (MQ=255)
                               cAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGtta                                                                                                                            >  3:638236/1‑142 (MQ=255)
                               cAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGtta                                                                                                                            <  4:638236/142‑1 (MQ=255)
                               cAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTc                                                                                                                     <  3:859572/149‑1 (MQ=255)
                               cAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTc                                                                                                                     <  3:889143/149‑1 (MQ=255)
                                aaaCGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCg                                                                                                                    >  2:381995/1‑149 (MQ=255)
                                    gctgacgctgacCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGa                                                                                                                <  2:453596/149‑1 (MQ=255)
                                          gctgacCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTc                                                                                                          <  2:103366/149‑1 (MQ=255)
                                          gctgacCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTc                                                                                                          >  3:317159/1‑149 (MQ=255)
                                               ccTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTc                                                                                                     <  1:1046983/149‑1 (MQ=255)
                                                 tttAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCttttt                                                                                                                                        >  1:966401/1‑113 (MQ=255)
                                                 tttAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCttt                                                                                                                                          <  2:837841/111‑1 (MQ=255)
                                                 tttAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCttt                                                                                                                                          >  1:837841/1‑111 (MQ=255)
                                                 tttAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCACTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCttttt                                                                                                                                        <  2:966401/113‑1 (MQ=255)
                                                       aGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTg                                                                                             >  3:591433/1‑149 (MQ=255)
                                                           gCGGTGTGTTGGCCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTTCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGaa                                                                                         <  3:9617/149‑1 (MQ=255)
                                                              gtgtgtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGct                                                                                      >  3:935103/1‑149 (MQ=255)
                                                                   ttGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTc                                                                                 <  4:310314/149‑1 (MQ=255)
                                                                   ttGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTc                                                                                 <  1:3529/149‑1 (MQ=255)
                                                                        cAATATTGATAACAAGCCTGCGCTGAGTGGTAACTCAAAATAAAGTTGCTCTCAAATACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATc                                                                            <  4:685697/149‑1 (MQ=255)
                                                                        cAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCACAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATc                                                                            >  1:686453/1‑149 (MQ=255)
                                                                        cAATATTGATAACAAACCTGCGATGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTTTTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATc                                                                            >  2:426483/1‑149 (MQ=255)
                                                                         aaTATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTAGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCg                                                                           <  3:233806/149‑1 (MQ=255)
                                                                            atTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGc                                                                                       >  2:920657/1‑134 (MQ=255)
                                                                               gATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAAt                                                                      >  2:717427/1‑148 (MQ=255)
                                                                               gATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAAt                                                                      <  2:64591/148‑1 (MQ=255)
                                                                               gATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAAt                                                                      <  1:717427/148‑1 (MQ=255)
                                                                               gATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAAt                                                                      >  1:64591/1‑148 (MQ=255)
                                                                                 tAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATc                                                                            <  3:470825/140‑1 (MQ=255)
                                                                                 tAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATgag                                                                   >  2:303487/1‑149 (MQ=255)
                                                                                  aaCAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATgaga                                                                  >  3:580487/1‑149 (MQ=255)
                                                                                  aaCAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATgaga                                                                  <  1:381995/149‑1 (MQ=255)
                                                                                  aaCAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATgaga                                                                  >  1:838424/1‑149 (MQ=255)
                                                                                  aaCAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATgaga                                                                  >  1:608457/1‑149 (MQ=255)
                                                                                  aaCAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATgaga                                                                  >  2:1061995/1‑149 (MQ=255)
                                                                                   aCAAACCTGCGCTGCGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGagag                                                                 >  4:172348/1‑149 (MQ=255)
                                                                                   aCAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGagag                                                                 >  4:445180/1‑149 (MQ=255)
                                                                                   aCAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGagag                                                                 >  1:498328/1‑149 (MQ=255)
                                                                                     aaaCCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAggg                                                               >  2:857086/1‑149 (MQ=255)
                                                                                     aaaCCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAgg                                                                >  4:532042/1‑148 (MQ=255)
                                                                                      aaCCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGc                                                              <  3:550552/149‑1 (MQ=255)
                                                                                       aCCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATc                                                                            <  1:46653/134‑1 (MQ=255)
                                                                                       aCCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGCCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATc                                                                            >  2:46653/1‑134 (MQ=255)
                                                                                        ccTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGa                                                            <  3:441636/149‑1 (MQ=255)
                                                                                         cTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGAt                                                           >  1:407285/1‑149 (MQ=255)
                                                                                           gcgcTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATaa                                                         <  4:524779/149‑1 (MQ=255)
                                                                                             gcTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAt                                                       <  1:454386/149‑1 (MQ=255)
                                                                                              cTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAtc                                                      <  1:1057736/149‑1 (MQ=255)
                                                                                               tGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAtct                                                     >  4:462594/1‑149 (MQ=255)
                                                                                                gAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAtctc                                                    <  1:303487/149‑1 (MQ=255)
                                                                                                     gTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAGAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCAccc                                               >  1:516014/1‑149 (MQ=255)
                                                                                                     gTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCAccc                                               >  2:509953/1‑149 (MQ=255)
                                                                                                      tAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTc                                                                                 <  2:669501/114‑1 (MQ=255)
                                                                                                      tAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTc                                                                                 >  1:669501/1‑114 (MQ=255)
                                                                                                          taataataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCg                                          >  3:444156/1‑149 (MQ=255)
                                                                                                          taataataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCg                                          >  4:501778/1‑149 (MQ=255)
                                                                                                          taataataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCg                                          >  1:887332/1‑149 (MQ=255)
                                                                                                             taataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGt                                                                                                   >  3:983698/1‑89 (MQ=255)
                                                                                                             taataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGt                                                                                                   <  4:983698/89‑1 (MQ=255)
                                                                                                                taaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCcatca                                    >  3:1016868/1‑149 (MQ=255)
                                                                                                                 aaaGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCcatcat                                   <  2:686453/149‑1 (MQ=255)
                                                                                                                    gTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGAt                                                                             <  1:528367/104‑1 (MQ=255)
                                                                                                                    gTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGAt                                                                             >  2:528367/1‑104 (MQ=255)
                                                                                                                         tctcAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCtt                                                                                   >  3:698799/1‑93 (MQ=255)
                                                                                                                         tctcAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGagag                                                                 <  4:164625/111‑1 (MQ=255)
                                                                                                                         tctcAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTa                           >  3:781985/1‑149 (MQ=255)
                                                                                                                         tctcAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTa                           >  4:664483/1‑149 (MQ=255)
                                                                                                                         tctcAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTCTCTGCTCGTCGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTa                           >  4:73299/1‑149 (MQ=255)
                                                                                                                           tcAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGc                                 <  2:643934/141‑1 (MQ=255)
                                                                                                                           tcAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATc                         >  2:96582/1‑149 (MQ=255)
                                                                                                                              aagaagTTAAAAAAGGGGCCCCATTAGCACCATTTTTCTGTCTGTTATTTATTTGCTGAGCTTTCTTCTCGTTGCCTGCGAAGCTCTTTAGGCTCGGCTACGAGAGGGCGATAAATCTCCAACCGATCGCCATCATGCACACTATCGCt                      >  2:353158/1‑149 (MQ=255)
                                                                                                                              aaGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTc                                                                                 <  3:999503/90‑1 (MQ=255)
                                                                                                                                   gTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGtt                 >  2:302490/1‑149 (MQ=255)
                                                                                                                                   gTTAAAAAAGGGGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTAATCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGTATCTGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCCCTTAGtt                 >  3:524167/1‑149 (MQ=255)
                                                                                                                                     tAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGtttt               <  1:988870/149‑1 (MQ=255)
                                                                                                                                     gaaaaaaGGTGCTCATTGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGtttt               >  3:337167/2‑149 (MQ=255)
                                                                                                                                       aaaaaGGTGCTCATTGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCa                                                  <  2:469359/112‑1 (MQ=255)
                                                                                                                                       aaaaaGGTGCTCATTGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCa                                                  >  1:469359/1‑112 (MQ=255)
                                                                                                                                       aaaaaGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTTTTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCAGATCGCCATCATGCACACTATCGCTTAGTTTTGc             >  1:874673/1‑149 (MQ=255)
                                                                                                                                       aaaaaGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGc             <  3:73299/149‑1 (MQ=255)
                                                                                                                                        aaaaGGTGCTCATTGAGCACCTTTTTTAACGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGc             >  2:169438/1‑149 (MQ=255)
                                                                                                                                                cTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCt    <  1:941283/149‑1 (MQ=255)
                                                                                                                                                  cAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGt  >  1:121096/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGT  >  NC_000913/2753838‑2754132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: