Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,777,571 A→G 7.6% D40G (GAC→GGC)  ypjF → CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,777,5710AG 7.6% 433.9 / 18.9 145D40G (GAC→GGC) ypjFCP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base A (55/78);  new base G (7/4);  total (62/83)
Fisher's exact test for biased strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

Only 100 of 149 total aligned reads displayed.

TCAACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATA  >  NC_000913/2777423‑2777715
                                                                                                                                                    |                                                                                                                                                
tgaaCGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCCGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGa                                                                                                                                                  >  2:850079/3‑149 (MQ=255)
tCAACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGACCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGa                                                                                                                                                  >  3:818229/1‑149 (MQ=255)
   aCGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGacac                                                                                                                                               <  3:857629/149‑1 (MQ=255)
     gCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGc                                                                                                                                             <  1:542252/149‑1 (MQ=255)
       taactaaTTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCg                                                                                                                                           >  2:664962/1‑149 (MQ=255)
       taactaaTTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAACCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCg                                                                                                                                           >  3:655449/1‑149 (MQ=255)
          ctaaTTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTc                                                                                                                                        <  3:445295/149‑1 (MQ=255)
             aTTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGt                                                                                                                                     <  2:601956/149‑1 (MQ=255)
              tttCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGtg                                                                                                                                    >  4:69999/1‑149 (MQ=255)
                  aCGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGtgatga                                                                                                                                >  1:272410/1‑149 (MQ=255)
                    gagaGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGaaa                                                                                                                              <  3:304980/149‑1 (MQ=255)
                     agagCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAAc                                                                                                                             <  4:33453/149‑1 (MQ=255)
                        gcaagcaTGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGt                                                                                                                          <  4:446276/149‑1 (MQ=255)
                          aagcaTGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGtta                                                                                                                        >  2:939643/1‑149 (MQ=255)
                          aagcaTGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGtta                                                                                                                        >  2:350341/1‑149 (MQ=255)
                           agcaTGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGttat                                                                                                                       <  2:412852/149‑1 (MQ=255)
                            gcaTGAACGCTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGttatt                                                                                                                      >  3:966595/1‑149 (MQ=255)
                            gcaTGAACACTCTACCTGCTACAATTTCGCAGGCTGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACGGttatt                                                                                                                      >  3:700668/1‑149 (MQ=255)
                            gcaTGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGttatt                                                                                                                      >  2:734941/1‑149 (MQ=255)
                              aTGATCACACTACCTGCTACAATTTCGCAGGCGGCGAAGTCCTGCCTATCGCCAGTGGCTGTCTGGCAAATGCTACTCACACGCCTGCTCGAACAACTCTATGGCCTGACACTGAACGACACGTCGCTCAGTGATGAAACTGTTATTaa                                                                                                                    <  4:655036/149‑1 (MQ=255)
                              aTGAACACTCTACCTGCTACAATTTCGCAGGTGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTaa                                                                                                                    <  2:460130/149‑1 (MQ=255)
                              aTGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCTGTGATGAAACTGTTATTaa                                                                                                                    <  4:889851/149‑1 (MQ=255)
                              aTGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTaa                                                                                                                    <  1:220298/149‑1 (MQ=255)
                               tGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAg                                                                                                                   <  3:883003/149‑1 (MQ=255)
                                 aaCACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTTACACGCCTGCTGGAACAACACTATGGCCTGTCACTGACCGTCACGCCTTTTAGCGATGAAACTGTTATTCAggt                                                                                                                 <  4:854112/149‑2 (MQ=255)
                                  acacTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGaa                                                                                                                <  2:279454/149‑1 (MQ=255)
                                    acTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACa                                                                                                              >  1:627919/1‑149 (MQ=255)
                                     ctctACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACat                                                                                                             <  4:735952/149‑1 (MQ=255)
                                      tctACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACata                                                                                                            >  2:225750/1‑149 (MQ=255)
                                      tctACCTGCTACAATATCGCATGCGGCGAAACCCTACCTGTCGCCAGTGGCTGTCTGGCAAATGCTACAGACACGCCTGATGGAAGAACTCTATGGCCTGACACTGAACGACTCGCCGTTCAACGATGAAACTGTTATTAAGGAACata                                                                                                            >  4:652068/1‑149 (MQ=255)
                                         aCCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCa                                                                                                                                       <  1:946445/119‑1 (MQ=255)
                                         aCCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCa                                                                                                                                       >  2:946445/1‑119 (MQ=255)
                                              cTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCt                                                                                                    >  3:115262/1‑149 (MQ=255)
                                               tACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTg                                                                                                   >  2:793443/1‑149 (MQ=255)
                                                 cAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGt                                                                                                 <  1:589684/149‑1 (MQ=255)
                                                  aaTTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTa                                                                                                >  1:438641/1‑149 (MQ=255)
                                                  aaTTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTa                                                                                                >  4:72689/1‑149 (MQ=255)
                                                  aaTTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTGCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTa                                                                                                <  3:298009/149‑1 (MQ=255)
                                                          aGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTg                                                                                        <  3:949991/149‑1 (MQ=255)
                                                          aGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTg                                                                                        >  4:949991/1‑149 (MQ=255)
                                                              ggcgAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGtt                                                                                                                         <  2:155630/112‑1 (MQ=255)
                                                                 gAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGGCGTGTCGTTCAGtgtca                                                                                                                                 <  4:1010479/101‑4 (MQ=38)
                                                                 gAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATg                                                                                 <  1:225750/149‑1 (MQ=255)
                                                                  aaGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATgt                                                                                <  1:389819/149‑2 (MQ=255)
                                                                    gCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAg                                                                              <  1:1006632/149‑1 (MQ=255)
                                                                     cccTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAAAAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGt                                                                             <  1:82748/149‑1 (MQ=255)
                                                                        tgcctgTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTTGTATCACTCTGGCCGATGCAGTCaa                                                                          <  4:1036252/149‑1 (MQ=255)
                                                                                gCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCtgg                                                                  <  2:115305/149‑1 (MQ=255)
                                                                                gCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCtgg                                                                  >  2:476723/1‑149 (MQ=255)
                                                                                 ccAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCtggt                                                                 <  4:966595/149‑1 (MQ=255)
                                                                                      ggCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGaaa                                                            <  2:980099/149‑1 (MQ=255)
                                                                                          gTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTa                                                        <  3:46653/149‑1 (MQ=255)
                                                                                           tCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACAGTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTAc                                                       <  2:877690/149‑1 (MQ=255)
                                                                                             tGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGa                                                     >  3:60974/1‑149 (MQ=255)
                                                                                             tGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGa                                                     >  2:810348/1‑149 (MQ=255)
                                                                                             tGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGa                                                     >  1:582429/1‑149 (MQ=255)
                                                                                             tGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGa                                                     >  1:975354/1‑149 (MQ=255)
                                                                                               gCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGacc                                                   <  1:381083/149‑3 (MQ=255)
                                                                                               gCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAAc                                                   >  4:640099/1‑149 (MQ=255)
                                                                                                 aaaTGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTg                                                 <  1:350341/149‑1 (MQ=255)
                                                                                                     gatACTGACACGCCTGATGCAACAACACCATGGCATGACACTGAACGACACGCCGTTCAGTGATGAAACTGCTATTAATGAAAATATCGATGCTGGTATCACCCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTAc                                             <  4:1032756/147‑1 (MQ=255)
                                                                                                      cTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACg                                            >  2:746599/1‑149 (MQ=255)
                                                                                                       tACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAg                                                                                                                   >  4:791047/1‑77 (MQ=255)
                                                                                                       tACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAg                                                                                                                   <  3:791047/77‑1 (MQ=255)
                                                                                                       tACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGt                                           >  4:662505/1‑149 (MQ=255)
                                                                                                         cTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACa                                                                                                              >  2:519439/1‑80 (MQ=255)
                                                                                                          tGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATc                                        >  4:360506/1‑149 (MQ=255)
                                                                                                            acacGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTg                                                                                                   >  1:167929/1‑88 (MQ=255)
                                                                                                             cacGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGAt                                     <  1:939643/149‑1 (MQ=255)
                                                                                                              acGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATc                                    <  2:26616/149‑1 (MQ=255)
                                                                                                                 ccTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACCTATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATcaca                                 <  3:714207/149‑1 (MQ=255)
                                                                                                                 ccTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATcaca                                 >  2:210095/1‑149 (MQ=255)
                                                                                                                   tgctgGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACaga                               <  2:985437/149‑1 (MQ=255)
                                                                                                                   tgctgGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGACGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCTCaga                               <  1:930429/149‑1 (MQ=255)
                                                                                                                      tgGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGa                            >  3:219038/1‑149 (MQ=255)
                                                                                                                       ggAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGAt                                                                                  >  1:553579/1‑94 (MQ=255)
                                                                                                                       ggAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGAt                                                                                  <  2:553579/94‑1 (MQ=255)
                                                                                                                          acaacaCTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCt                                                                    >  3:651238/1‑105 (MQ=255)
                                                                                                                           caacaCTATGGCCTGACACTGAACGATACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGTTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGCAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTc                       <  4:607656/149‑1 (MQ=255)
                                                                                                                           caacaCTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTc                       <  4:779630/149‑1 (MQ=255)
                                                                                                                            aacaCTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAAc                                                   >  1:264728/1‑120 (MQ=255)
                                                                                                                            aacaCTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAAc                                                   <  2:264728/120‑1 (MQ=255)
                                                                                                                            aacaCTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCg                      >  1:639149/1‑149 (MQ=255)
                                                                                                                              cacTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTg                    <  3:38121/149‑1 (MQ=255)
                                                                                                                               acTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTgg                   <  3:338231/149‑1 (MQ=255)
                                                                                                                                cTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGc                  <  1:810348/149‑1 (MQ=255)
                                                                                                                                 tATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAAc                                                   >  4:1034044/1‑115 (MQ=255)
                                                                                                                                 tATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAAc                                                   <  3:1034044/115‑1 (MQ=255)
                                                                                                                                       cTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAacaaca           <  3:532174/149‑1 (MQ=255)
                                                                                                                                        tGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAg          >  1:705809/1‑149 (MQ=255)
                                                                                                                                          acacTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGtc        >  2:448393/1‑149 (MQ=255)
                                                                                                                                           cacTGAACGGCGTGTCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGtct       >  1:249886/1‑149 (MQ=255)
                                                                                                                                           cacTGAACGGCGTGTCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGtct       >  1:107917/1‑149 (MQ=255)
                                                                                                                                            acTGAACGGCGTGTCGTTCAGTGATGAAACTGTTATTAAGTAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGAt                                     <  4:417673/118‑1 (MQ=255)
                                                                                                                                            acTGAACGGCGTGTCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGtctc      >  1:731146/1‑149 (MQ=255)
                                                                                                                                            acTGAACGGCGTGTCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGtctc      >  3:181970/1‑149 (MQ=255)
                                                                                                                                            acTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTc                       >  1:872367/1‑132 (MQ=255)
                                                                                                                                            acTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGtctc      <  1:1057144/149‑1 (MQ=255)
                                                                                                                                            acTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGAAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTc                       <  2:872367/132‑1 (MQ=255)
                                                                                                                                              tGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCa    <  2:144156/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TCAACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATA  >  NC_000913/2777423‑2777715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: