Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 374,689 A→G 5.8% R274R (CGA→CGG) ‡ mhpE → 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913374,6890AG 5.8% 273.7 / 5.4 86R274R (CGA→CGG) ‡mhpE4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I
Reads supporting (aligned to +/- strand):  ref base A (42/39);  new base G (2/3);  total (44/42)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.30e-01

CGGGAATGGGCGCGGGCGCAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGG  >  NC_000913/374541‑374837
                                                                                                                                                    |                                                                                                                                                    
cgGGAATGGGCGCGGGCGCAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGa                                                                                                                                                      <  2:297752/149‑1 (MQ=255)
cgGGAATGGGCGCGGGCGCAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGa                                                                                                                                                      <  1:250956/149‑1 (MQ=255)
       gggcgcgggcgcAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACTCGTACCGGTCGATc                                                                                                                                               <  1:71992/149‑1 (MQ=255)
         gcgcgggcgcAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATcgc                                                                                                                                             >  2:16749/1‑149 (MQ=255)
         gcgcgggcgcAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATcgc                                                                                                                                             >  1:93705/1‑149 (MQ=255)
             gggcgcAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGaaa                                                                                                                                         <  4:547262/149‑1 (MQ=255)
              ggcgcAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGTATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAAc                                                                                                                                        <  1:759560/149‑1 (MQ=255)
                 gcAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAAcgct                                                                                                                                     <  2:701409/149‑1 (MQ=255)
                 gcAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAAcgct                                                                                                                                     >  2:665904/1‑149 (MQ=255)
                  cAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAAcgctg                                                                                                                                    <  2:93705/149‑1 (MQ=255)
                    ggTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAAcgctggc                                                                                                                                  >  4:223300/1‑149 (MQ=255)
                         cGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAAcgctggcgctgg                                                                                                                             <  3:129518/149‑1 (MQ=255)
                          gCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGAGTCGTACCGGTCGATcct                                                                                                                                             >  3:855437/1‑130 (MQ=255)
                          gCACCGCTGGAAGTGTTTATCGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTggg                                                                                                                            <  2:529971/149‑1 (MQ=255)
                          gCACCGCTGGAAGTGATTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACTCGTACCGGTCGATcct                                                                                                                                             <  4:855437/132‑3 (MQ=255)
                           cACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGa                                                                                                                                                      >  1:40358/1‑122 (MQ=255)
                           cACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGa                                                                                                                                                      <  2:40358/122‑1 (MQ=255)
                            aCCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATc                                                                                                                                               >  1:965128/1‑128 (MQ=255)
                            aCCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATc                                                                                                                                               <  2:965128/128‑1 (MQ=255)
                              cGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACTCGTACCGGTCGATcct                                                                                                                                             <  4:293024/128‑3 (MQ=255)
                              cGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACTCGTACCGGTCGATcct                                                                                                                                             >  3:293024/1‑126 (MQ=255)
                                 tGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCt                                                                                                                     >  1:48759/1‑149 (MQ=255)
                                  ggAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTg                                                                                                                    <  3:240388/149‑1 (MQ=255)
                                    aaGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGgt                                                                                                                  <  2:916030/149‑1 (MQ=255)
                                    aaGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGgt                                                                                                                  <  1:377023/149‑1 (MQ=255)
                                       tgtTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGtt                                                                                                               <  2:928703/149‑1 (MQ=255)
                                       tgtTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGtt                                                                                                               >  1:85230/1‑149 (MQ=255)
                                         tttATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTa                                                                                                             >  1:101788/1‑149 (MQ=255)
                                         tttATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTa                                                                                                             >  4:157194/1‑149 (MQ=255)
                                         tttATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTa                                                                                                             <  1:14043/149‑1 (MQ=255)
                                         tttATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTa                                                                                                             >  1:952437/1‑149 (MQ=255)
                                         tttATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTa                                                                                                             >  3:450455/1‑149 (MQ=255)
                                          ttATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTAc                                                                                                            <  4:853273/149‑1 (MQ=255)
                                              tGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGa                                                                                                        <  1:321516/149‑1 (MQ=255)
                                                ccgccgCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGc                                                                                                      <  4:243036/149‑1 (MQ=255)
                                                ccgccgCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGc                                                                                                      <  1:446270/149‑1 (MQ=255)
                                                  gccgcGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCtt                                                                                                    >  1:317916/1‑149 (MQ=255)
                                                       ggATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGc                                                                                               >  4:557028/1‑149 (MQ=255)
                                                       ggATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGc                                                                                               <  3:292936/149‑1 (MQ=255)
                                                        gATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCg                                                                                              <  4:102173/149‑1 (MQ=255)
                                                           aaaCTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGa                                                                                                                                                      >  2:143570/1‑90 (MQ=255)
                                                           aaaCTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGa                                                                                                                                                      <  1:143570/90‑1 (MQ=255)
                                                           aaaCTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCa                                                                                           >  4:290566/1‑149 (MQ=255)
                                                            aaCTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCAc                                                                                          >  1:277803/1‑149 (MQ=255)
                                                            aaCTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCAc                                                                                          >  4:195918/1‑149 (MQ=255)
                                                            aaCTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCAc                                                                                          <  3:816975/149‑1 (MQ=255)
                                                               tGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACtgt                                                                                       <  1:69004/149‑1 (MQ=255)
                                                                 ggCTGTCAGCAGGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGa                                                                                     >  4:672723/1‑149 (MQ=255)
                                                                    tGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAAcgctggcgctgg                                                                                                                             <  1:157824/106‑1 (MQ=255)
                                                                    tGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAAcgctggcgctgg                                                                                                                             >  2:157824/1‑106 (MQ=255)
                                                                    tGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAAc                                                                                  <  2:774215/149‑1 (MQ=255)
                                                                    tGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAAc                                                                                  <  4:63457/149‑1 (MQ=255)
                                                                            tGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAAcggcggcgg                                                                          >  3:925993/1‑149 (MQ=255)
                                                                            tGGGACCGATCTCTATGCGTAAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAAcggcggcgg                                                                          >  3:5603/1‑149 (MQ=255)
                                                                                  cGATCTCGATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGtt                                                                    >  2:928680/1‑149 (MQ=255)
                                                                                    aTCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTAt                                                                  >  4:210005/1‑149 (MQ=255)
                                                                                      ctctATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATgg                                                                <  1:72518/149‑1 (MQ=255)
                                                                                         tATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTAc                                                                                                            >  1:401256/1‑102 (MQ=255)
                                                                                         tATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTAc                                                                                                            <  2:401256/102‑1 (MQ=255)
                                                                                         tATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCtt                                                             >  3:713239/1‑149 (MQ=255)
                                                                                         tATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCtt                                                             >  1:45181/1‑149 (MQ=255)
                                                                                          aTGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTa                                                            >  2:508799/1‑149 (MQ=255)
                                                                                          aTGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTa                                                            >  1:448753/1‑149 (MQ=255)
                                                                                              gTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTg                                                        >  1:687194/1‑149 (MQ=255)
                                                                                               ttAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGc                                                       >  3:704553/1‑149 (MQ=255)
                                                                                                  aTGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATgg                                                                <  1:161020/137‑1 (MQ=255)
                                                                                                  aTGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATgg                                                                >  2:161020/1‑137 (MQ=255)
                                                                                                  aTGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGt                                                    <  3:512376/149‑1 (MQ=255)
                                                                                                     gATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGa                                                 <  3:186033/149‑1 (MQ=255)
                                                                                                       tGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGata                                               <  2:112178/149‑1 (MQ=255)
                                                                                                          cgccgACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCGGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATtc                                            <  3:695652/149‑1 (MQ=255)
                                                                                                            ccgACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATtctc                                          >  1:1037622/1‑149 (MQ=255)
                                                                                                            ccgACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATtctc                                          <  1:135797/149‑1 (MQ=255)
                                                                                                            ccgACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATtctc                                          <  2:906488/149‑1 (MQ=255)
                                                                                                            ccgACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATtctc                                          <  3:210005/149‑1 (MQ=255)
                                                                                                            ccgACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATtctc                                          <  3:287292/149‑1 (MQ=255)
                                                                                                             cgacgaCCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCg                                         <  3:445819/149‑1 (MQ=255)
                                                                                                              gacgacCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGt                                        >  1:699773/1‑149 (MQ=255)
                                                                                                                    cTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCt                                  >  3:220621/1‑149 (MQ=255)
                                                                                                                    cTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCt                                  <  1:859896/149‑1 (MQ=255)
                                                                                                                      ggTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTgg                                <  3:377838/149‑1 (MQ=255)
                                                                                                                           gTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTCGAGCTGGGCaaa                           >  2:657712/1‑149 (MQ=255)
                                                                                                                            tCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAAc                          >  2:305974/1‑149 (MQ=255)
                                                                                                                            tCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAAc                          >  4:59367/1‑149 (MQ=255)
                                                                                                                                    aGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATg                  >  1:536664/1‑149 (MQ=255)
                                                                                                                                    aGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATg                  >  4:785539/1‑149 (MQ=255)
                                                                                                                                       aTCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGtt               <  1:665904/149‑1 (MQ=255)
                                                                                                                                           aCCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTggcg           >  4:1034227/1‑149 (MQ=255)
                                                                                                                                                tACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCag      >  3:884937/1‑149 (MQ=255)
                                                                                                                                                  cGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCGGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCagga    <  4:713239/149‑1 (MQ=255)
                                                                                                                                                   gAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCaggag   >  2:719564/1‑149 (MQ=255)
                                                                                                                                                   gAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCaggag   >  1:460714/1‑149 (MQ=255)
                                                                                                                                                    aGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCaggagg  <  4:76390/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGGGAATGGGCGCGGGCGCAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGG  >  NC_000913/374541‑374837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: