Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,418,628 G→A 6.0% intergenic (+17/‑414) yhdV → / → yhdW putative outer membrane protein/pseudogene, ABC transporter periplasmic binding protein family;putative transport; Not classified; putative periplasmic binding transport protein; periplasmic‑binding component of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,418,6280GA 6.0% 314.5 / 7.3 100intergenic (+17/‑414)yhdV/yhdWputative outer membrane protein/pseudogene, ABC transporter periplasmic binding protein family;putative transport; Not classified; putative periplasmic binding transport protein; periplasmic‑binding component of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base G (58/36);  new base A (2/4);  total (60/40)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01

Only 100 of 101 total aligned reads displayed.

GGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTCACAC  >  NC_000913/3418480‑3418771
                                                                                                                                                    |                                                                                                                                               
ggTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAg                                                                                                                                                 >  2:547448/1‑149 (MQ=255)
    gtcTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTcc                                                                                                                                             <  4:411252/149‑1 (MQ=255)
     tcTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCcg                                                                                                                                            >  4:405154/1‑149 (MQ=255)
       tGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCcgcg                                                                                                                                          >  2:629794/1‑149 (MQ=255)
        ggTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCgcgc                                                                                                                                         >  4:1021066/1‑149 (MQ=255)
         gTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGct                                                                                                                                        >  3:51453/1‑149 (MQ=255)
          ttCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGctc                                                                                                                                       >  1:173262/1‑149 (MQ=255)
          ttCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAACTCCgcgccc                                                                                                                                       <  2:479324/149‑3 (MQ=255)
           tCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGctct                                                                                                                                      <  4:202309/149‑1 (MQ=255)
           tCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAACTCCGCGccct                                                                                                                                      <  4:1031186/149‑1 (MQ=255)
             acacCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCttt                                                                                                                                    >  1:929697/1‑149 (MQ=255)
              cacCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCtttt                                                                                                                                   <  2:945654/149‑1 (MQ=255)
              cacCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCtttt                                                                                                                                   <  2:756316/149‑1 (MQ=255)
               acCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCttttt                                                                                                                                  >  3:137794/1‑149 (MQ=255)
                ccAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTa                                                                                                                                 >  2:538981/1‑149 (MQ=255)
                ccAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTa                                                                                                                                 >  4:506308/1‑149 (MQ=255)
                ccAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAACTCCGCGCCCtgttca                                                                                                                                 <  2:99594/149‑3 (MQ=255)
                 cAATGCCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTaa                                                                                                                                >  1:905915/1‑149 (MQ=255)
                 cAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTaa                                                                                                                                <  2:567313/149‑1 (MQ=255)
                 cAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACACGGCGCGGAGTTCCGCGCTCTTTTTaa                                                                                                                                <  3:651790/149‑1 (MQ=255)
                   aTACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACg                                                                                                                              >  1:605837/1‑149 (MQ=255)
                       cTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATAc                                                                                                                          >  3:343105/1‑149 (MQ=255)
                       cTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATAc                                                                                                                          >  4:814138/1‑149 (MQ=255)
                       cTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATAc                                                                                                                          >  1:1035828/1‑149 (MQ=255)
                       cTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATAc                                                                                                                          >  1:330783/1‑149 (MQ=255)
                        tGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGAGACa                                                                                                                         >  4:906591/1‑149 (MQ=255)
                           aTGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTa                                                                                                                      >  1:234915/1‑149 (MQ=255)
                           aTGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTa                                                                                                                      >  2:345265/1‑149 (MQ=255)
                            tGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTAt                                                                                                                     <  4:137794/149‑1 (MQ=255)
                                aGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGctc                                                                                                                                       <  1:429960/127‑1 (MQ=255)
                                aGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGctc                                                                                                                                       >  2:429960/1‑127 (MQ=255)
                                aGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTAtctct                                                                                                                 >  3:840026/1‑149 (MQ=255)
                                    aCGACTCATGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCAt                                                                                                             >  3:738878/1‑149 (MQ=255)
                                    aCGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCAt                                                                                                             >  3:128245/1‑149 (MQ=255)
                                    aCGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCAt                                                                                                             >  3:460682/1‑149 (MQ=255)
                                         tCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGAt                                                                                                        >  3:282093/1‑149 (MQ=255)
                                         tCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGAt                                                                                                        >  1:678910/1‑149 (MQ=255)
                                         tCAGGATAACTGGGAAACGGCTGGGCCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGAt                                                                                                        <  1:851041/149‑1 (MQ=255)
                                           aGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATaa                                                                                                      >  2:360812/1‑149 (MQ=255)
                                              aTAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAat                                                                                                   >  1:735188/1‑149 (MQ=255)
                                                 aCTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATAtt                                                                                                >  1:814350/1‑149 (MQ=255)
                                                  cTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTg                                                                                               <  1:419731/149‑1 (MQ=255)
                                                  cTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTg                                                                                               <  2:624837/149‑1 (MQ=255)
                                                    gggAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCt                                                                                             <  2:852657/149‑1 (MQ=255)
                                                     ggAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCta                                                                                            <  2:1035828/149‑1 (MQ=255)
                                                             cTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTctgc                                                                                    <  4:387248/149‑1 (MQ=255)
                                                             cTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTctgc                                                                                    <  3:1021066/149‑1 (MQ=255)
                                                              tGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTctgct                                                                                   <  1:629794/149‑1 (MQ=255)
                                                                gggCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGctc                                                                                                                                       <  1:371011/95‑1 (MQ=255)
                                                                gggCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGctc                                                                                                                                       >  2:371011/1‑95 (MQ=255)
                                                                gggCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATa                                                                                                                           >  2:494558/1‑107 (MQ=255)
                                                                gggCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATa                                                                                                                           <  1:494558/107‑1 (MQ=255)
                                                                gggCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTgg                                                                                 <  4:671995/149‑1 (MQ=255)
                                                                 ggCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTggg                                                                                <  1:360812/149‑1 (MQ=255)
                                                                  gCTATTGCCGGCGGGGCGGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCCCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGt                                                                               >  2:271531/1‑149 (MQ=255)
                                                                  gCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTaa                                                                                                                                <  1:408900/100‑1 (MQ=255)
                                                                  gCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTaa                                                                                                                                >  2:408900/1‑100 (MQ=255)
                                                                  gCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGt                                                                               <  1:343364/149‑1 (MQ=255)
                                                                  gCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGt                                                                               <  3:506308/149‑1 (MQ=255)
                                                                    tATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGa                                                                                                         >  3:93055/1‑121 (MQ=255)
                                                                    tATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTaa                                                                             <  3:405154/149‑1 (MQ=255)
                                                                     aTTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAAt                                                                            <  1:1007680/149‑1 (MQ=255)
                                                                     aTTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCAGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAAt                                                                            <  4:671292/149‑1 (MQ=255)
                                                                       tGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTc                                                                          >  3:114025/1‑149 (MQ=255)
                                                                          cggcggGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCct                                                                       <  4:425704/149‑1 (MQ=255)
                                                                            gcggGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCctgc                                                                     >  1:373266/1‑149 (MQ=255)
                                                                                  cAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCct                                                                       <  1:252254/141‑1 (MQ=255)
                                                                                  cAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCct                                                                       >  2:252254/1‑141 (MQ=255)
                                                                                  cAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGAttt                                                               >  3:277070/1‑149 (MQ=255)
                                                                                      tgctgTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCa                                                           >  3:683873/1‑149 (MQ=255)
                                                                                           ttGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTa                                                      >  4:324794/1‑149 (MQ=255)
                                                                                           ttGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTa                                                      <  2:288478/149‑1 (MQ=255)
                                                                                           ttGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTa                                                      >  2:970249/1‑149 (MQ=255)
                                                                                                 gCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATcgc                                                >  4:929564/1‑149 (MQ=255)
                                                                                                    tGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGtt                                             >  1:858013/1‑149 (MQ=255)
                                                                                                    tGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGtt                                             >  4:1042844/1‑149 (MQ=255)
                                                                                                           ggggATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCgc                                      >  2:801058/1‑149 (MQ=255)
                                                                                                             ggATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCgctg                                    >  4:643031/1‑149 (MQ=255)
                                                                                                             ggATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCgctg                                    >  2:436198/1‑149 (MQ=255)
                                                                                                              gATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCgctgc                                   <  2:691716/149‑1 (MQ=255)
                                                                                                              gATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCgctgc                                   >  1:691716/1‑149 (MQ=255)
                                                                                                                  atcGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCNTTATTTCGCTGCTTTc                               >  2:355455/1‑149 (MQ=255)
                                                                                                                  atcGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTc                               <  3:814138/149‑1 (MQ=255)
                                                                                                                          aTCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAAc                       >  2:988308/1‑149 (MQ=255)
                                                                                                                          aTCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAAc                       >  1:817693/1‑149 (MQ=255)
                                                                                                                          aTCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAAc                       <  3:929564/149‑1 (MQ=255)
                                                                                                                           tCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACg                      >  1:144976/1‑149 (MQ=255)
                                                                                                                              aaaTAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAGATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCAt                   >  2:294732/1‑149 (MQ=255)
                                                                                                                               aaTAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATc                  >  1:835056/1‑149 (MQ=255)
                                                                                                                                aTAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCa                 >  2:537898/1‑149 (MQ=255)
                                                                                                                                  aaCTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCaat               >  2:836334/1‑149 (MQ=255)
                                                                                                                                    cTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCaattt             <  1:541761/149‑1 (MQ=255)
                                                                                                                                    cTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCaattt             >  2:152163/1‑149 (MQ=255)
                                                                                                                                        aaagagcgcGGAACTCCGCGCCCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCaatttaatt         >  3:396114/6‑149 (MQ=255)
                                                                                                                                          agagcgcGGAACTCCGCGCCCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAAtttt       <  4:396114/146‑1 (MQ=255)
                                                                                                                                           gggCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTc      <  4:282093/149‑1 (MQ=255)
                                                                                                                                           gagcgcGGAACTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTc      >  4:417848/3‑149 (MQ=255)
                                                                                                                                            ggCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTca     <  2:835056/149‑1 (MQ=255)
                                                                                                                                             gcgcGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTcac    >  1:548150/1‑149 (MQ=255)
                                                                                                                                               gcgGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTCacac  >  1:534222/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTCACAC  >  NC_000913/3418480‑3418771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: