Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,885,305 G→T 5.5% G77V (GGT→GTT)  yidC → membrane protein insertase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,885,3050GT 5.5% 442.6 / 9.1 127G77V (GGT→GTT) yidCmembrane protein insertase
Reads supporting (aligned to +/- strand):  ref base G (56/63);  new base T (4/3);  total (60/67)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 132 total aligned reads displayed.

ACCCGCAACCTCAGGCCCAACAGACCACGCAGACAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGATGGCCCGGATAACCCGGCTAACGG  >  NC_000913/3885158‑3885452
                                                                                                                                                   |                                                                                                                                                   
 cccGCAACCTCAGGCTCAACAGACCACGCAGACAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCgtggtggtg                                                                                                                                                   <  3:994601/149‑1 (MQ=255)
  ccGCAACCTCAGGCCCAACAGACCACGCAGACAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGa                                                                                                                                                  >  3:759814/1‑149 (MQ=255)
   cGCAACCTCAGGCCCAACAGACCACGCAGACAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGAt                                                                                                                                                 >  3:193241/1‑149 (MQ=255)
    gCAACCTCAGGCCCAACAGACCACGCAGACAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCACCACCACGGGTGTTGATg                                                                                                                                                <  2:428764/149‑1 (MQ=255)
    gCAACCTCAGGCCCAACAGACCACGCAGACAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATg                                                                                                                                                >  3:203614/1‑149 (MQ=255)
     cAACCTCAGGCCCAACAGACCACGCAGACAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGt                                                                                                                                               <  2:832652/149‑1 (MQ=255)
     cAACCTCAGGCCCAACAGACCACGCAGACAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGt                                                                                                                                               <  2:507991/149‑1 (MQ=255)
      aaCCTCAGGCCGAACAGACCACGCAGACAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAGCTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGtt                                                                                                                                              <  2:461889/149‑1 (MQ=255)
        ccTCAGGCCCAACAGACCACGCAGACAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGa                                                                                                                                            <  3:152297/149‑1 (MQ=255)
          tCAGGCCCAACAGACCACGCAGACAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCACCACCACGGGTGTTGATggtcaga                                                                                                                                          <  3:643063/149‑7 (MQ=255)
            aGGCCCAACAGACCACGCAGACAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCaa                                                                                                                                        >  1:210274/1‑149 (MQ=255)
                 cAACAGACCACGCAGACAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGGGGTGATGTTGAGCAAGctct                                                                                                                                   >  4:366508/1‑149 (MQ=255)
                  aaCAGACCACGCAGACAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTctg                                                                                                                                  >  4:333955/1‑149 (MQ=255)
                   aCAGACCACGCAGACAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTctgc                                                                                                                                 <  3:311060/149‑1 (MQ=255)
                      gACCACGCAGACAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCtgctgc                                                                                                                              <  4:25984/149‑1 (MQ=255)
                           cGCAGACAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCt                                                                                                                         >  1:223966/1‑149 (MQ=255)
                            gCAGACAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCACCACCACGGGTGTTGATg                                                                                                                                                <  2:805736/125‑1 (MQ=255)
                              aGACAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTAc                                                                                                                      <  3:129396/149‑1 (MQ=255)
                                aCAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTAccc                                                                                                                    >  2:396796/1‑149 (MQ=255)
                                aCAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTAccc                                                                                                                    <  1:828578/149‑1 (MQ=255)
                                 cAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCg                                                                                                                   >  2:976027/1‑149 (MQ=255)
                                  aaCGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGtt                                                                                                                                              >  1:355806/1‑121 (MQ=255)
                                  aaCGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGtt                                                                                                                                              <  2:355806/121‑1 (MQ=255)
                                  aaCGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGa                                                                                                                  >  3:554080/1‑149 (MQ=255)
                                  aaCGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGa                                                                                                                  >  1:461945/1‑149 (MQ=255)
                                    cGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGaaa                                                                                                                <  3:947332/149‑1 (MQ=255)
                                    cGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGaaa                                                                                                                <  2:475405/149‑1 (MQ=255)
                                      accaccGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAaga                                                                                                              >  1:723949/1‑149 (MQ=255)
                                         accGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCt                                                                                                           <  2:543410/149‑1 (MQ=255)
                                         accGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCt                                                                                                           >  3:597278/1‑149 (MQ=255)
                                          ccGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTg                                                                                                          <  4:431011/149‑1 (MQ=255)
                                            gCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGaa                                                                                                        <  3:111009/149‑1 (MQ=255)
                                            gCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGaa                                                                                                        <  4:446731/149‑1 (MQ=255)
                                             cAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAAc                                                                                                       <  4:597278/149‑1 (MQ=255)
                                                 gggTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGctgcc                                                                                                                             >  4:50202/1‑123 (MQ=255)
                                                 gggTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGctgcc                                                                                                                             <  3:50202/123‑1 (MQ=255)
                                                            ccgACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGATTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACTCGAAAGAGCTGAACTCTACCCAGCCGTTc                                                                                        <  3:897676/149‑1 (MQ=255)
                                                               aCCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAg                                                                                     <  3:363719/149‑1 (MQ=255)
                                                                  aGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGACGTTCCAGCTg                                                                                  >  4:374326/1‑149 (MQ=255)
                                                                   gggCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTa                                                                                                   >  1:56497/1‑131 (MQ=255)
                                                                   gggCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCCGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGt                                                                                 <  3:720460/149‑1 (MQ=255)
                                                                    ggCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGtt                                                                                <  3:374326/149‑1 (MQ=255)
                                                                    ggCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGtt                                                                                <  3:526166/149‑1 (MQ=255)
                                                                     gCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTg                                                                               <  4:726550/149‑1 (MQ=255)
                                                                       gTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCCTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGaaa                                                                                                                <  2:189906/114‑1 (MQ=255)
                                                                       gTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCCTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGaaa                                                                                                                >  1:189906/1‑114 (MQ=255)
                                                                          ccGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAAc                                                                          <  2:1334/149‑1 (MQ=255)
                                                                               cAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCAc                                                                     <  2:456416/149‑1 (MQ=255)
                                                                               cAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCAGCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCAc                                                                     <  4:644740/149‑1 (MQ=255)
                                                                                 gTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCg                                                                   >  3:589837/1‑149 (MQ=255)
                                                                                     ccAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGt                                                               >  1:653588/1‑149 (MQ=255)
                                                                                      cAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCa                                                                      >  3:867292/1‑141 (MQ=255)
                                                                                      cAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCa                                                                      <  4:867292/141‑1 (MQ=255)
                                                                                         gggAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGtttat                                                           <  1:74077/149‑1 (MQ=255)
                                                                                           gAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGtttattt                                                         >  3:401344/1‑149 (MQ=255)
                                                                                              aCTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATc                                                      <  2:1022222/149‑1 (MQ=255)
                                                                                               cTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCa                                                     <  1:380586/149‑1 (MQ=255)
                                                                                                   tctcGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGca                                                 >  3:515863/1‑149 (MQ=255)
                                                                                                   tctcGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGca                                                 >  1:995992/1‑149 (MQ=255)
                                                                                                     tcGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGcaca                                               >  1:331695/1‑149 (MQ=255)
                                                                                                        gTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACagag                                            >  4:287692/1‑149 (MQ=255)
                                                                                                            aGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGt                                        <  1:323451/149‑1 (MQ=255)
                                                                                                            aGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGt                                        >  3:934303/1‑149 (MQ=255)
                                                                                                             gACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAgg                                                   <  3:31408/137‑1 (MQ=255)
                                                                                                                cGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGa                                    <  4:554080/149‑1 (MQ=255)
                                                                                                                cGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGa                                    <  4:737437/149‑1 (MQ=255)
                                                                                                                cGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGa                                    <  3:127804/149‑1 (MQ=255)
                                                                                                                cGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGa                                    >  3:346531/1‑149 (MQ=255)
                                                                                                                      gCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTc                              <  1:577720/149‑1 (MQ=255)
                                                                                                                        ttGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGt                            >  3:113876/1‑149 (MQ=255)
                                                                                                                        ttGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGt                            >  2:680877/1‑149 (MQ=255)
                                                                                                                         tGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTATCAGGCACAGAGCGGTCTGACCGGTCGTGa                          <  3:413619/149‑1 (MQ=255)
                                                                                                                          gATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGa                          >  1:636444/1‑149 (MQ=255)
                                                                                                                          gATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGa                          <  4:277257/149‑1 (MQ=255)
                                                                                                                           aTCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGtttatt                                                          <  1:592336/116‑1 (MQ=255)
                                                                                                                           aTCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGtttatt                                                          >  2:592336/1‑116 (MQ=255)
                                                                                                                           aTCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGAt                         >  1:393305/1‑149 (MQ=255)
                                                                                                                           aTCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGAt                         >  1:130144/1‑149 (MQ=255)
                                                                                                                             cTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGATgg                       <  4:65593/149‑1 (MQ=255)
                                                                                                                              tcaacATCACCACCACGGGTGTTGATGTTGAGGAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGATGGc                      >  4:898085/5‑149 (MQ=255)
                                                                                                                              tcaacATCACCACCACGGGTGTTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGATGGc                      >  4:562768/5‑149 (MQ=255)
                                                                                                                              tGACCATCAACACCCGGGGTGGTGATGTTTAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGATGGc                      >  4:812107/1‑149 (MQ=255)
                                                                                                                               gACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGATGGcc                     <  3:316372/149‑1 (MQ=255)
                                                                                                                               gACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGATGGcc                     <  1:499837/149‑1 (MQ=255)
                                                                                                                                aCCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGATGGccc                    <  3:149030/149‑1 (MQ=255)
                                                                                                                                aCCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGATGGccc                    <  3:287692/149‑1 (MQ=255)
                                                                                                                                aCCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGATGGccc                    <  1:274250/149‑1 (MQ=255)
                                                                                                                                  cATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGttta                                                            <  1:752115/107‑1 (MQ=255)
                                                                                                                                  cATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGttta                                                            >  2:752115/1‑107 (MQ=255)
                                                                                                                                  cATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGATGGCCCgg                  >  1:659023/1‑149 (MQ=255)
                                                                                                                                  cATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGATGGCCCgg                  >  4:546953/1‑149 (MQ=255)
                                                                                                                                  cATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGATGGCCCgg                  >  1:497337/1‑149 (MQ=255)
                                                                                                                                   aTCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCt                                                                                   >  1:940965/1‑83 (MQ=255)
                                                                                                                                   aTCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCt                                                                                   <  2:940965/83‑1 (MQ=255)
                                                                                                                                    tCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATTAGGCACAGAGCGGTCTGACCGGTCGTGATGGCCCGGAt                >  2:704250/1‑149 (MQ=255)
                                                                                                                                         acCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGcc                                                                                            >  4:583338/1‑68 (MQ=255)
                                                                                                                                         acCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGATGGCCCGGATAAccc           >  2:896921/1‑149 (MQ=255)
                                                                                                                                         acCCGTGGTGGTGATGTTGAGCAAGCTCTACTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGATGGCCCGGATAAccc           >  1:86475/1‑149 (MQ=255)
                                                                                                                                           ccGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGATGGCCCGGATAACCCgg         >  2:441514/1‑149 (MQ=255)
                                                                                                                                                  ggtgATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGATGGCCCGGATAACCCGGCTAACgg  >  2:887057/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ACCCGCAACCTCAGGCCCAACAGACCACGCAGACAACGACCACCGCAGCGGGTAGCGCCGCCGACCAGGGCGTACCGGCCAGTGGCCAGGGGAAACTGATCTCGGTTAAGACCGACGTGCTTGATCTGACCATCAACACCCGTGGTGGTGATGTTGAGCAAGCTCTGCTGCCTGCTTACCCGAAAGAGCTGAACTCTACCCAGCCGTTCCAGCTGTTGGAAACTTCACCGCAGTTTATTTATCAGGCACAGAGCGGTCTGACCGGTCGTGATGGCCCGGATAACCCGGCTAACGG  >  NC_000913/3885158‑3885452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: