Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,897,613 G→A 6.6% P36P (CCG→CCA cbrB → PRK09823 family inner membrane protein, creBC regulon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,897,6130GA 6.6% 587.8 / 20.1 182P36P (CCG→CCAcbrBPRK09823 family inner membrane protein, creBC regulon
Reads supporting (aligned to +/- strand):  ref base G (78/92);  new base A (7/5);  total (85/97)
Fisher's exact test for biased strand distribution p-value = 5.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

Only 100 of 183 total aligned reads displayed.

CGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGC  >  NC_000913/3897465‑3897760
                                                                                                                                                    |                                                                                                                                                   
cGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCg                                                                                                                                                     >  4:167193/1‑149 (MQ=255)
   ttAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTg                                                                                                                                                  <  2:792476/149‑1 (MQ=255)
   ttAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTg                                                                                                                                                  >  1:586320/1‑149 (MQ=255)
    tAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTgg                                                                                                                                                 <  1:661018/149‑1 (MQ=255)
      aaTTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGtt                                                                                                                                               >  3:151271/1‑149 (MQ=255)
         tGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCtt                                                                                                                                            <  4:374151/149‑1 (MQ=255)
         tGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCtt                                                                                                                                            <  3:687751/149‑1 (MQ=255)
          ggCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCttt                                                                                                                                           <  4:634078/149‑1 (MQ=255)
           gcgcTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTg                                                                                                                                          <  1:38642/149‑1 (MQ=255)
           gcgcTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCAGCGGTTCtttt                                                                                                                                          <  3:345087/149‑2 (MQ=255)
           gcgcTAAAGGGTAAGTAGGGGGAGTTAGATATGTCTGTTGCACGTCGGGTAACACATCACGGACTATATTTTCCAGATTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTg                                                                                                                                          >  2:181912/1‑149 (MQ=255)
            cgcTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTgg                                                                                                                                         <  3:850597/149‑1 (MQ=255)
             gcTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTggg                                                                                                                                        <  2:837751/149‑1 (MQ=255)
              cTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGt                                                                                                                                       >  4:428413/1‑149 (MQ=255)
               tCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTgg                                                                                                                                         <  3:508854/146‑1 (MQ=255)
               tCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTg                                                                                                                                      >  2:50379/1‑149 (MQ=255)
               tCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTg                                                                                                                                      >  2:537091/1‑149 (MQ=255)
               tCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTg                                                                                                                                      >  3:115754/1‑149 (MQ=255)
               tCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTg                                                                                                                                      >  4:129964/1‑149 (MQ=255)
               tCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGAGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTgg                                                                                                                                         >  4:508854/1‑146 (MQ=255)
                   aGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGataa                                                                                                                                  <  1:79047/149‑1 (MQ=255)
                          taaAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTACAGTTTTAGGACAGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATcc                                                                                                                           >  4:1019820/1‑149 (MQ=255)
                             aGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAAt                                                                                                                        <  1:149896/149‑1 (MQ=255)
                                   tttGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTtct                                                                                                                  >  1:161464/1‑149 (MQ=255)
                                   tttGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTtct                                                                                                                  <  2:196321/149‑1 (MQ=255)
                                       atatGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTa                                                                                                              <  1:549359/149‑1 (MQ=255)
                                            tCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGTCCGTTACTTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTc                                                                                                         >  1:982198/1‑149 (MQ=255)
                                            tCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTc                                                                                                         <  4:216216/149‑1 (MQ=255)
                                            tCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTc                                                                                                         >  4:927114/1‑149 (MQ=255)
                                            tCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTc                                                                                                         >  1:1012654/1‑149 (MQ=255)
                                               gTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGAt                                                                                                      <  3:546797/149‑1 (MQ=255)
                                                  tCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTc                                                                                                         <  2:543839/143‑1 (MQ=255)
                                                  tCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTc                                                                                                         >  1:543839/1‑143 (MQ=255)
                                                      gTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACg                                                                                               <  3:637228/149‑1 (MQ=255)
                                                      gTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACg                                                                                               >  3:79292/1‑149 (MQ=255)
                                                       tCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACgg                                                                                              <  1:199187/149‑1 (MQ=255)
                                                         gggTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGa                                                                                            <  3:940570/149‑1 (MQ=255)
                                                         gggTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGa                                                                                            >  1:312241/1‑149 (MQ=255)
                                                          ggTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAg                                                                                           >  1:642132/1‑149 (MQ=255)
                                                            tAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTGTTGTCCTCTACCTATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTAAGGGAGCt                                                                                         <  2:11500/149‑1 (MQ=255)
                                                            tAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAgtt                                                                                         <  4:721369/149‑3 (MQ=255)
                                                                acgtCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATCTTTCTCTTATTGTCGATAACTACGGGAGCTACTc                                                                                     <  3:927114/146‑1 (MQ=255)
                                                                  aTCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTgg                                                                                                                                         >  4:302123/1‑95 (MQ=255)
                                                                    cACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGc                                                                                 <  4:151271/149‑1 (MQ=255)
                                                                     aCGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGataa                                                                                                                                  <  2:694872/99‑1 (MQ=255)
                                                                     aCGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAAttttt                                                                                                                    <  1:920355/113‑1 (MQ=255)
                                                                       ggACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgtt                                                                              <  2:1012406/149‑1 (MQ=255)
                                                                        gACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgttg                                                                             >  1:947900/1‑149 (MQ=255)
                                                                         aCTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgtt                                                                              >  1:218485/1‑147 (MQ=255)
                                                                         aCTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgtt                                                                              <  2:218485/147‑1 (MQ=255)
                                                                          cTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgttgtt                                                                           <  1:725409/149‑1 (MQ=255)
                                                                           tttATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTa                                                                          >  2:945253/1‑149 (MQ=255)
                                                                            ttATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTaa                                                                         <  2:6729/149‑1 (MQ=255)
                                                                             tATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAAc                                                                        <  3:488243/149‑1 (MQ=255)
                                                                                 ttGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGgt                                                                    >  4:68266/1‑149 (MQ=255)
                                                                                  tGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTCATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGgtg                                                                   <  2:143165/149‑1 (MQ=255)
                                                                                       ttttAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGgt                                                                    <  4:574082/143‑1 (MQ=255)
                                                                                       ttttAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGgt                                                                    >  3:574082/1‑143 (MQ=255)
                                                                                       ttttAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATg                                                              <  2:586320/149‑1 (MQ=255)
                                                                                       ttttAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATg                                                              >  4:19046/1‑149 (MQ=255)
                                                                                       ttttAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATg                                                              >  1:543321/1‑149 (MQ=255)
                                                                                            gtaCCGTTAATTGGTCTTCTGGCTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTTGTTCTTTGGGTGTTAATCTATACACTTTTTCTCTTAGTGTCCATAACTACGGGAGCGATACCCGCGTTGTTGCCCGGTGTAATGGTTgg                                                         <  2:982198/147‑2 (MQ=255)
                                                                                            ggACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGc                                                         <  3:19046/149‑1 (MQ=255)
                                                                                              aCCGTTAATTGGTGTTCTGTTTCTCGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCt                                                       <  2:766937/149‑1 (MQ=255)
                                                                                               ccGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTg                                                      >  1:366530/1‑149 (MQ=255)
                                                                                                 gTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTc                                                    >  2:828285/1‑149 (MQ=255)
                                                                                                     aTTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTCTAATGGTTGCCTGTCTGcc                                                <  4:917773/149‑1 (MQ=255)
                                                                                                      ttGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCg                                               >  2:105867/1‑149 (MQ=255)
                                                                                                            ttCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAg                                         >  1:720293/1‑149 (MQ=255)
                                                                                                            ttCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAg                                         >  2:991946/1‑149 (MQ=255)
                                                                                                               tGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATc                                      <  3:943229/149‑1 (MQ=255)
                                                                                                                gTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCg                                     <  4:79292/149‑1 (MQ=255)
                                                                                                                 tttCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCgg                                    >  4:669939/1‑149 (MQ=255)
                                                                                                                 tttCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCgg                                    <  2:161464/149‑1 (MQ=255)
                                                                                                                 tttCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCgg                                    >  3:373276/1‑149 (MQ=255)
                                                                                                                 tttCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCgg                                    >  3:303874/1‑149 (MQ=255)
                                                                                                                 tttCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTGCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCgg                                    <  4:832712/149‑1 (MQ=255)
                                                                                                                        tCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgt                                                                               >  3:624917/1‑99 (MQ=255)
                                                                                                                        tCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAAt                                                               >  3:97083/1‑115 (MQ=255)
                                                                                                                        tCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAg                             >  4:51042/1‑149 (MQ=255)
                                                                                                                          ctctACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCgg                                    <  2:287726/140‑1 (MQ=255)
                                                                                                                          ctctACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGaa                           >  4:960123/1‑149 (MQ=255)
                                                                                                                            ctACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTc                                                                                     >  2:317736/1‑89 (MQ=255)
                                                                                                                            ctACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAAc                         >  1:619934/1‑149 (MQ=255)
                                                                                                                             tACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACg                        >  3:87507/1‑149 (MQ=255)
                                                                                                                                atatTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGc                                                 <  2:721059/121‑1 (MQ=255)
                                                                                                                                atatTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTa                     <  1:1061059/149‑1 (MQ=255)
                                                                                                                                  atTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATc                   <  2:642132/149‑1 (MQ=255)
                                                                                                                                   ttcttcGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCg                  >  1:110559/1‑149 (MQ=255)
                                                                                                                                     cttcGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGtt                <  2:619934/149‑1 (MQ=255)
                                                                                                                                     atatcccaaaGAACCAGCGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGtt                <  3:287087/142‑1 (MQ=255)
                                                                                                                                         gCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAATGATCGGGTCACAGAAACGTTATCGTTGTct            >  1:691207/1‑149 (MQ=255)
                                                                                                                                          ccaaaGAACCAGCGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTctg           >  2:402270/3‑149 (MQ=255)
                                                                                                                                          ccaaaGAACCAGCGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTctg           >  2:573449/3‑149 (MQ=255)
                                                                                                                                           caaaGAACCAGCGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTGCGGGAGCTATTCCTGCGTTGTTAACCGGTGTAAt                                                               >  1:815107/2‑96 (MQ=255)
                                                                                                                                           caaaGAACCAGCGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAAt                                                               <  2:815107/95‑1 (MQ=255)
                                                                                                                                           caaaGAACCAGCGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTctgg          >  2:530583/2‑149 (MQ=255)
                                                                                                                                            aaaGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTctggc         <  2:366530/149‑1 (MQ=255)
                                                                                                                                            aaaGAACCAGCGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTctggc         >  1:10502/1‑149 (MQ=255)
                                                                                                                                                aaCCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGCGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCtggt     <  3:1040636/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGC  >  NC_000913/3897465‑3897760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: