Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,897,614 C→G 6.5% L37V (CTG→GTG) ‡ cbrB → PRK09823 family inner membrane protein, creBC regulon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,897,6140CG 6.5% 630.0 / 25.3 182L37V (CTG→GTG) ‡cbrBPRK09823 family inner membrane protein, creBC regulon
Reads supporting (aligned to +/- strand):  ref base C (78/92);  new base G (7/5);  total (85/97)
Fisher's exact test for biased strand distribution p-value = 5.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 182 total aligned reads displayed.

TTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGC  >  NC_000913/3897468‑3897760
                                                                                                                                                  |                                                                                                                                                  
ttAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTg                                                                                                                                                  <  2:792476/149‑1 (MQ=255)
ttAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTg                                                                                                                                                  >  1:586320/1‑149 (MQ=255)
   aaTTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGtt                                                                                                                                               >  3:151271/1‑149 (MQ=255)
      tGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCtt                                                                                                                                            <  4:374151/149‑1 (MQ=255)
      tGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCtt                                                                                                                                            <  3:687751/149‑1 (MQ=255)
       ggCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCttt                                                                                                                                           <  4:634078/149‑1 (MQ=255)
        gcgcTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTg                                                                                                                                          <  1:38642/149‑1 (MQ=255)
        gcgcTAAAGGGTAAGTAGGGGGAGTTAGATATGTCTGTTGCACGTCGGGTAACACATCACGGACTATATTTTCCAGATTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTg                                                                                                                                          >  2:181912/1‑149 (MQ=255)
         cgcTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTgg                                                                                                                                         <  4:20080/149‑1 (MQ=255)
          gcTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTggg                                                                                                                                        <  2:837751/149‑1 (MQ=255)
           cTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGt                                                                                                                                       <  2:536896/149‑1 (MQ=255)
           cTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGt                                                                                                                                       >  4:428413/1‑149 (MQ=255)
            tCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTgg                                                                                                                                         <  3:508854/146‑1 (MQ=255)
            tCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTg                                                                                                                                      >  2:50379/1‑149 (MQ=255)
            tCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGAGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTgg                                                                                                                                         >  4:508854/1‑146 (MQ=255)
                aGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGataa                                                                                                                                  <  1:79047/149‑1 (MQ=255)
                    aagtaaAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACa                                                                                                                              <  3:1029809/149‑1 (MQ=255)
                         aaGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCaa                                                                                                                         <  3:990714/149‑1 (MQ=255)
                          aGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAAt                                                                                                                        >  3:927375/1‑149 (MQ=255)
                          aGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAAt                                                                                                                        <  1:149896/149‑1 (MQ=255)
                                tttGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTtct                                                                                                                  >  3:832712/1‑149 (MQ=255)
                                tttGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTtct                                                                                                                  >  1:161464/1‑149 (MQ=255)
                                tttGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTtct                                                                                                                  <  2:196321/149‑1 (MQ=255)
                                   gATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACa                                                                                                                              <  2:658435/134‑1 (MQ=255)
                                   gATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACa                                                                                                                              >  1:658435/1‑134 (MQ=255)
                                   gATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCtt                                                                                                               >  2:50848/1‑149 (MQ=255)
                                    atatGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTa                                                                                                              <  1:549359/149‑1 (MQ=255)
                                      atGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTAtt                                                                                                            >  3:616474/1‑149 (MQ=255)
                                        gTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGt                                                                                                          >  4:131268/1‑149 (MQ=255)
                                         tCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGTCCGTTACTTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTc                                                                                                         >  1:982198/1‑149 (MQ=255)
                                         tCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTc                                                                                                         >  4:927114/1‑149 (MQ=255)
                                         tCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTc                                                                                                         <  2:245927/149‑1 (MQ=255)
                                         tCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTc                                                                                                         <  4:216216/149‑1 (MQ=255)
                                         tCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTc                                                                                                         >  1:1012654/1‑149 (MQ=255)
                                               tCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTc                                                                                                         >  1:543839/1‑143 (MQ=255)
                                                 aCGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTa                                                                                                 >  2:812025/1‑149 (MQ=255)
                                                   gTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACg                                                                                               >  3:79292/1‑149 (MQ=255)
                                                    tCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACgg                                                                                              <  3:11468/149‑1 (MQ=255)
                                                    tCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACgg                                                                                              <  1:199187/149‑1 (MQ=255)
                                                      gggTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGa                                                                                            >  1:312241/1‑149 (MQ=255)
                                                      gggTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGa                                                                                            <  2:691463/149‑1 (MQ=255)
                                                       ggTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAg                                                                                           <  1:889015/149‑1 (MQ=255)
                                                       ggTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAg                                                                                           >  1:642132/1‑149 (MQ=255)
                                                         tAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAgtt                                                                                         <  4:721369/149‑3 (MQ=255)
                                                               aTCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTgg                                                                                                                                         <  3:302123/95‑1 (MQ=255)
                                                               aTCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTgg                                                                                                                                         >  4:302123/1‑95 (MQ=255)
                                                               aTCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCt                                                                                   >  2:12168/1‑149 (MQ=255)
                                                                    ggACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgtt                                                                              <  2:312241/149‑1 (MQ=255)
                                                                    ggACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgtt                                                                              <  2:1012406/149‑1 (MQ=255)
                                                                     gACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgttg                                                                             >  1:947900/1‑149 (MQ=255)
                                                                      aCTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgtt                                                                              <  2:218485/147‑1 (MQ=255)
                                                                      aCTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgtt                                                                              >  1:218485/1‑147 (MQ=255)
                                                                       cTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTAtt                                                                                      >  2:991534/1‑138 (MQ=255)
                                                                       cTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgttgtt                                                                           <  1:725409/149‑1 (MQ=255)
                                                                        tttATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTa                                                                          >  2:945253/1‑149 (MQ=255)
                                                                          tATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAAc                                                                        <  3:488243/149‑1 (MQ=255)
                                                                              ttGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGgt                                                                    >  4:68266/1‑149 (MQ=255)
                                                                              ttGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTGCTTGTGCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTCTTAACCGgt                                                                    >  3:55799/1‑149 (MQ=255)
                                                                                    ttttAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGgt                                                                    <  4:574082/143‑1 (MQ=255)
                                                                                    ttttAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATg                                                              >  1:543321/1‑149 (MQ=255)
                                                                                    ttttAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATg                                                              >  3:327140/1‑149 (MQ=255)
                                                                                    ttttAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATg                                                              <  2:586320/149‑1 (MQ=255)
                                                                                         gtaCCGTTAATTGGTCTTCTGGCTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTTGTTCTTTGGGTGTTAATCTATACACTTTTTCTCTTAGTGTCCATAACTACGGGAGCGATACCCGCGTTGTTGCCCGGTGTAATGGTTgg                                                         <  2:982198/147‑2 (MQ=255)
                                                                                         ggACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGc                                                         <  3:19046/149‑1 (MQ=255)
                                                                                           aCCGTTAATTGGTGTTCTGTTTCTCGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCt                                                       <  2:766937/149‑1 (MQ=255)
                                                                                            ccGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTg                                                      >  1:366530/1‑149 (MQ=255)
                                                                                              gTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTc                                                    >  3:968795/1‑149 (MQ=255)
                                                                                                  aTTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTCTAATGGTTGCCTGTCTGcc                                                <  4:917773/149‑1 (MQ=255)
                                                                                                   ttGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCg                                               >  2:105867/1‑149 (MQ=255)
                                                                                                         ttCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAg                                         >  1:720293/1‑149 (MQ=255)
                                                                                                           cTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGAt                                       >  2:826126/1‑149 (MQ=255)
                                                                                                            tGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATc                                      <  3:943229/149‑1 (MQ=255)
                                                                                                             gTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCg                                     <  4:79292/149‑1 (MQ=255)
                                                                                                              tttCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCgg                                    <  2:161464/149‑1 (MQ=255)
                                                                                                              tttCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCgg                                    <  3:867203/149‑1 (MQ=255)
                                                                                                              tttCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCgg                                    >  4:669939/1‑149 (MQ=255)
                                                                                                              tttCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTGCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCgg                                    <  4:832712/149‑1 (MQ=255)
                                                                                                                  ttGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTca                                >  3:545983/1‑149 (MQ=255)
                                                                                                                  ttGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCCGCCGGAAAAGATCGGGTca                                <  3:916064/149‑1 (MQ=255)
                                                                                                                     tCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAg                             >  4:51042/1‑149 (MQ=255)
                                                                                                                       ctctACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGaa                           >  4:960123/1‑149 (MQ=255)
                                                                                                                        tctACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGaaa                          >  3:630373/1‑149 (MQ=255)
                                                                                                                         ctACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAAc                         >  1:619934/1‑149 (MQ=255)
                                                                                                                           aCATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGTCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGtt                      >  4:157601/1‑149 (MQ=255)
                                                                                                                            cATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGtt                      <  2:620941/149‑1 (MQ=255)
                                                                                                                             atatTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGc                                                 <  2:721059/121‑1 (MQ=255)
                                                                                                                             atatTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTa                     <  1:1061059/149‑1 (MQ=255)
                                                                                                                               atTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATc                   <  2:543321/149‑1 (MQ=255)
                                                                                                                                ttcttcGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCg                  >  1:110559/1‑149 (MQ=255)
                                                                                                                                  cttcGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGtt                <  2:619934/149‑1 (MQ=255)
                                                                                                                                  atatcccaaaGAACCAGCGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGtt                <  3:287087/142‑1 (MQ=255)
                                                                                                                                   ttcGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTg               >  3:350590/1‑149 (MQ=255)
                                                                                                                                    tcGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAAATCGGGTCACAGAAACGTTATCGTTGt              >  4:108994/1‑149 (MQ=255)
                                                                                                                                      gCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAATGATCGGGTCACAGAAACGTTATCGTTGTct            >  1:691207/1‑149 (MQ=255)
                                                                                                                                      gCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTc             >  2:1067185/1‑148 (MQ=255)
                                                                                                                                       ccaaaGAACCAGCGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTctg           >  2:573449/3‑149 (MQ=255)
                                                                                                                                       ccaaaGAACCAGCGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTctg           >  2:402270/3‑149 (MQ=255)
                                                                                                                                        caaaGAACCAGCGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTGCGGGAGCTATTCCTGCGTTGTTAACCGGTGTAAt                                                               >  1:815107/2‑96 (MQ=255)
                                                                                                                                         aaaGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTctggc         <  2:366530/149‑1 (MQ=255)
                                                                                                                                         aaaGAACCAGCGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTctggc         >  1:10502/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGC  >  NC_000913/3897468‑3897760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: