Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,923,376 | A→G | 5.1% | V102A (GTG→GCG) ‡ | rsmG ← | 16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,923,376 | 0 | A | G | 5.1% | 500.5 / 10.2 | 151 | V102A (GTG→GCG) ‡ | rsmG | 16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein |
Reads supporting (aligned to +/- strand): ref base A (78/65); new base G (2/6); total (80/71) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.48e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01 |
Only 100 of 160 total aligned reads displayed.
GCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATG > NC_000913/3923228‑3923524 | gCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAacgca > 2:177958/1‑149 (MQ=255) gCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGtt < 4:807327/149‑1 (MQ=255) gCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGtt > 1:772472/1‑149 (MQ=255) gCTCACCATATCGTTCAGAGAGGCAAAAGCGCGACTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGtt > 3:538930/1‑149 (MQ=255) cTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGttt < 2:509587/149‑1 (MQ=255) cACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCGTGCGtttcc < 1:828741/149‑3 (MQ=255) aCCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTAc > 4:223826/1‑148 (MQ=255) cATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCGTGCGtttccttc < 3:632812/149‑6 (MQ=255) cATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCGTGCGtttccttc < 3:418443/149‑6 (MQ=255) cATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCaa < 4:632394/149‑1 (MQ=255) cATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCaa > 1:979829/1‑149 (MQ=255) atatCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAg < 3:948033/149‑1 (MQ=255) atCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGc > 3:985124/1‑149 (MQ=255) ttCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATc < 4:1002865/149‑1 (MQ=255) agagagGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCaa > 1:606020/1‑149 (MQ=255) agagagGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCaa > 4:451066/1‑149 (MQ=255) agagGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACa > 1:1035595/1‑149 (MQ=255) agGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACaga < 3:271780/149‑1 (MQ=255) gCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCGTGCGtttcc > 3:222743/1‑128 (MQ=255) gCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGt > 1:440739/1‑149 (MQ=255) gCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACCCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGt < 2:815812/149‑1 (MQ=255) aaaaGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGa > 2:316843/1‑149 (MQ=255) aaGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGaaa < 4:902663/149‑1 (MQ=255) aaGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGaaa < 2:864753/149‑1 (MQ=255) gCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCtt < 1:177958/149‑1 (MQ=255) gCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCtt < 2:812221/149‑1 (MQ=255) cTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTc > 4:46109/1‑149 (MQ=255) tAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCa > 4:171573/1‑149 (MQ=255) aaTTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAg < 3:1042616/149‑1 (MQ=255) ttACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGa < 3:46109/149‑1 (MQ=255) gCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCa > 2:930760/1‑149 (MQ=255) aTCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGa > 1:680638/1‑149 (MQ=255) aTCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGa > 3:17303/1‑149 (MQ=255) tCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGAt > 4:762166/1‑149 (MQ=255) aaTGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCCGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATaga < 4:8288/149‑1 (MQ=255) gcggcTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGt > 2:270890/1‑149 (MQ=255) cggcTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTg < 1:516148/149‑1 (MQ=255) gcTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGa > 2:324182/1‑149 (MQ=255) tctGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAg < 1:101800/98‑1 (MQ=255) tctGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAg > 2:101800/1‑98 (MQ=255) gAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGcc < 2:440739/149‑1 (MQ=255) aaGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCt > 3:41382/1‑149 (MQ=255) attccaCTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTg > 2:276324/6‑149 (MQ=255) ggAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTgg > 1:403035/1‑149 (MQ=255) gAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGc > 1:376472/1‑149 (MQ=255) tcttctACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTcc < 1:617391/149‑1 (MQ=255) cttctACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCt < 2:430366/149‑1 (MQ=255) ttctACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTg > 2:619603/1‑149 (MQ=255) tctACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTgg < 1:295307/149‑1 (MQ=255) ctACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGt > 2:993966/1‑149 (MQ=255) aCCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTcc > 3:553601/1‑149 (MQ=255) aCCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTcc < 1:784918/149‑1 (MQ=255) aCCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTcc > 2:521262/1‑149 (MQ=255) cccTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCg > 2:881470/1‑149 (MQ=255) cccTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCg > 3:751136/1‑149 (MQ=255) ccTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGa < 4:683941/116‑1 (MQ=255) ccTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCgg > 2:594539/1‑149 (MQ=255) ccTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCgg > 3:23698/1‑149 (MQ=255) cTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGt > 1:344875/1‑149 (MQ=255) cTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGt < 2:505917/149‑1 (MQ=255) tGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGtt < 4:583171/69‑1 (MQ=255) tGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCATGGTCCGGTg > 2:349170/1‑149 (MQ=255) tctGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCg > 1:939148/1‑149 (MQ=255) acaggTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCGGACATc < 2:368701/146‑1 (MQ=255) gTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATa > 2:380226/1‑149 (MQ=255) ttCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATaa > 2:701613/1‑149 (MQ=255) tCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCt > 3:811066/1‑117 (MQ=255) aaTATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAAcc > 3:576212/1‑149 (MQ=255) atatTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCg > 3:72873/1‑149 (MQ=255) tatTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGt > 3:457459/1‑149 (MQ=255) ttCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTc < 3:371374/149‑1 (MQ=255) ctcCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCAc < 3:421456/149‑1 (MQ=255) tcCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATagaga < 4:160858/97‑1 (MQ=255) gTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCg < 2:499613/119‑1 (MQ=255) gTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGc > 3:670239/1‑149 (MQ=255) gCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATAc < 2:1035595/149‑1 (MQ=255) cATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGt < 1:881617/149‑1 (MQ=255) gTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCtt > 4:100168/1‑63 (MQ=255) gTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCtt < 3:100168/63‑1 (MQ=255) ttGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATc < 3:858093/117‑1 (MQ=255) ttGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATc > 4:858093/1‑117 (MQ=255) aCCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCcaccac > 3:73190/1‑149 (MQ=255) aCCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCcaccac > 3:292698/1‑149 (MQ=255) cTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACaa > 2:676197/1‑149 (MQ=255) tGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAAt < 4:73190/149‑1 (MQ=255) ttggtaaaCGCGTGCGTTTACCAAGGCTATTCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCtt > 2:614687/6‑123 (MQ=255) gAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGaa < 1:529/43‑1 (MQ=255) gAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGc < 2:981986/99‑1 (MQ=255) gAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGc > 1:981986/1‑99 (MQ=255) gAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTa > 4:376583/1‑149 (MQ=255) gAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTa > 4:360144/1‑149 (MQ=255) aGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATc > 4:804091/1‑149 (MQ=255) ggAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCg > 3:363932/1‑149 (MQ=255) gAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCga < 3:168073/149‑1 (MQ=255) acgcacgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGaa < 2:403035/149‑1 (MQ=255) gcacgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCa < 4:489958/117‑1 (MQ=255) gcacgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAata > 3:802904/1‑149 (MQ=255) acgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTcc > 2:46790/1‑77 (MQ=255) acgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTcc < 1:46790/77‑1 (MQ=255) acgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATg > 1:384777/1‑149 (MQ=255) | GCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATG > NC_000913/3923228‑3923524 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |