Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,953,432 C→T 5.2% intergenic (+19/‑46) ilvE → / → ilvD branched‑chain amino acid aminotransferase/dihydroxyacid dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,953,4320CT 5.2% 314.7 / 4.9 96intergenic (+19/‑46)ilvE/ilvDbranched‑chain amino acid aminotransferase/dihydroxyacid dehydratase
Reads supporting (aligned to +/- strand):  ref base C (43/48);  new base T (2/3);  total (45/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

GACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCG  >  NC_000913/3953288‑3953574
                                                                                                                                                |                                                                                                                                              
gACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGTGACGgcacgc                                                                                                                                            >  2:986474/1‑149 (MQ=255)
gACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGgcacgc                                                                                                                                            >  2:1016596/1‑149 (MQ=255)
      aTTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTc                                                                                                                                      <  3:108928/149‑1 (MQ=255)
       ttCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTcc                                                                                                                                     <  4:166057/149‑1 (MQ=255)
        tCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTccc                                                                                                                                    <  1:785628/149‑1 (MQ=255)
        tCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTccc                                                                                                                                    <  1:423002/149‑1 (MQ=255)
           ggTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGTGCGTGCCGTCCCAtt                                                                                                                                 <  1:339717/149‑1 (MQ=255)
              tGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTAc                                                                                                                              <  3:378035/149‑1 (MQ=255)
               ggCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACg                                                                                                                             <  2:812184/149‑1 (MQ=255)
               ggCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACg                                                                                                                             <  4:448275/149‑1 (MQ=255)
                gCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATGAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACga                                                                                                                            >  3:714692/1‑149 (MQ=255)
                gCGAAGGCCGTTGTGGACCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACga                                                                                                                            <  3:667872/149‑1 (MQ=255)
                  gAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGTGCGTGCCGTCCCAttttttgta                                                                                                                          <  2:384272/149‑7 (MQ=255)
                  gAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACgaga                                                                                                                          >  3:1033770/1‑149 (MQ=255)
                    aGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGagaca                                                                                                                        <  3:477937/149‑1 (MQ=255)
                    aGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGagaca                                                                                                                        <  2:855092/149‑1 (MQ=255)
                    aGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGagaca                                                                                                                        <  4:641232/149‑1 (MQ=255)
                            gtgGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTgg                                                                                                                <  3:834614/149‑1 (MQ=255)
                            gtgGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTgg                                                                                                                <  4:579904/149‑1 (MQ=255)
                              ggCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGa                                                                                                              <  2:565915/149‑1 (MQ=255)
                              ggCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGa                                                                                                              <  3:877125/149‑1 (MQ=255)
                              ggCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGa                                                                                                              <  4:66260/149‑1 (MQ=255)
                               gCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAg                                                                                                             >  2:343823/1‑149 (MQ=255)
                                 ccGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTc                                                                                                                                      <  1:1052102/122‑1 (MQ=255)
                                 ccGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTc                                                                                                                                      >  2:1052102/1‑122 (MQ=255)
                                 ccGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGacac                                                                                                                   <  3:122806/141‑1 (MQ=255)
                                 ccGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGacac                                                                                                                   >  4:122806/1‑141 (MQ=255)
                                 ccGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGta                                                                                                           >  3:47106/1‑149 (MQ=255)
                                     ttACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGtaaata                                                                                                       >  2:833480/1‑149 (MQ=255)
                                      tACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGtaaataa                                                                                                      <  4:733509/149‑1 (MQ=255)
                                       aCCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGtaaataaa                                                                                                     <  1:438493/149‑1 (MQ=255)
                                       aCCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGtaaataaa                                                                                                     <  4:824265/149‑1 (MQ=255)
                                          aaaCGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGGGAAAAAAAAgat                                                                                                  >  3:776924/1‑147 (MQ=255)
                                          aaaCGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTa                                                                                                  >  4:1043380/1‑149 (MQ=255)
                                          aaaCGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTa                                                                                                  <  3:769842/149‑1 (MQ=255)
                                          aaaCGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTa                                                                                                  >  4:767184/1‑149 (MQ=255)
                                           gatctaTTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACCCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTAt                                                                                                 <  3:549918/144‑1 (MQ=255)
                                           aaCGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGCCAGCCACTCGCAGtacataaacgat                                                                                                 <  2:975803/149‑5 (MQ=255)
                                           aaCGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTAt                                                                                                 <  1:535113/149‑1 (MQ=255)
                                                aTTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAGTGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTa                                                                                            >  2:843783/1‑149 (MQ=255)
                                                 ttCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTaa                                                                                           <  4:527729/149‑1 (MQ=255)
                                                 ttCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTaa                                                                                           <  1:343823/149‑1 (MQ=255)
                                                 ttCAGCAAGCCTTCGTCGGCCTCTTCACGGGCGAAACCGAAGATAAATGGGGCTAGTTAGAGCAGGTTAAGCAAGAAATACAAAAAATGGGGCGGCACGCACGGGCCCAGTTACGAGACAGACACGGGGAGGAAATAAAGTATgagatc                                                                                           >  4:549918/1‑144 (MQ=255)
                                                  tCAGCAAGCCTTCTTCGGCCTCTTCCCTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAg                                                                                          <  3:603578/149‑1 (MQ=255)
                                                   cAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGt                                                                                         <  1:990947/149‑1 (MQ=255)
                                                   cAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGt                                                                                         <  1:468304/149‑1 (MQ=255)
                                                      cAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACga                                                                                                                            >  2:404227/1‑111 (MQ=255)
                                                      cAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACga                                                                                                                            <  1:404227/111‑1 (MQ=255)
                                                      cAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACAGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTAcc                                                                                      <  2:912168/149‑1 (MQ=255)
                                                       aaGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGTGCGTGCCGTCCCAtttt                                                                                                                               >  1:473813/1‑106 (MQ=38)
                                                       aaGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGTGCGTGCCGTCCCAtttt                                                                                                                               <  2:473813/107‑2 (MQ=39)
                                                           cttcttCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTcc                                                                                 >  3:976049/1‑149 (MQ=255)
                                                           cttcttCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATAAAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTcc                                                                                 >  1:17531/1‑149 (MQ=255)
                                                              cttcGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGcc                                                                              <  4:900300/149‑1 (MQ=255)
                                                               ttcGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGcca                                                                             >  4:703991/1‑149 (MQ=255)
                                                               ttcGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGcca                                                                             >  2:479057/1‑149 (MQ=255)
                                                                     ctctTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTaa                                                                                           <  4:347352/129‑1 (MQ=255)
                                                                     ctctTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTaa                                                                                           >  3:347352/1‑129 (MQ=255)
                                                                       ctTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACt                                                                     >  1:884909/1‑149 (MQ=255)
                                                                          cACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCAt                                                                  <  1:319207/149‑1 (MQ=255)
                                                                           aCTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATg                                                                 >  2:597905/1‑149 (MQ=255)
                                                                           aCTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATg                                                                 <  2:1040892/149‑1 (MQ=255)
                                                                            cTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATgg                                                                >  3:199906/1‑149 (MQ=255)
                                                                             tGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGt                                                               >  4:387700/1‑149 (MQ=255)
                                                                                  aaaCCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTaa                                                          <  3:299560/149‑1 (MQ=255)
                                                                                          gATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCgg                                                  >  2:231745/1‑149 (MQ=255)
                                                                                          gATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCgg                                                  <  4:1033770/149‑1 (MQ=255)
                                                                                             aaaTGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTg                                               >  4:928257/1‑149 (MQ=255)
                                                                                               aTGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTTCt                                             >  4:590148/1‑149 (MQ=255)
                                                                                                tGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTc                                            <  4:29705/149‑1 (MQ=255)
                                                                                                 ggggCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCg                                           >  1:1027977/1‑149 (MQ=255)
                                                                                                 ggggCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCg                                           >  4:807418/1‑149 (MQ=255)
                                                                                                      tGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTgcgc                                      >  3:681100/1‑149 (MQ=255)
                                                                                                        gTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCtg                                    >  1:472398/1‑149 (MQ=255)
                                                                                                           aGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTgg                                 >  2:679331/1‑149 (MQ=255)
                                                                                                             aTCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTgg                                                                                                                <  2:90264/68‑1 (MQ=255)
                                                                                                             aTCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTgg                                                                                                                >  1:90264/1‑68 (MQ=255)
                                                                                                             aTCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgcg                               >  3:273965/1‑149 (MQ=255)
                                                                                                             aTCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgcg                               >  3:927460/1‑149 (MQ=255)
                                                                                                             aTCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgcg                               >  2:165047/1‑149 (MQ=255)
                                                                                                              tCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgcgc                              >  1:85180/1‑149 (MQ=255)
                                                                                                              tCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgcgc                              >  2:688765/1‑149 (MQ=255)
                                                                                                                      aTCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCgg                      >  4:416288/1‑149 (MQ=255)
                                                                                                                       tCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGa                     >  1:158051/1‑149 (MQ=255)
                                                                                                                          ataaataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATg                  <  4:714692/149‑1 (MQ=255)
                                                                                                                           taaataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGa                 <  2:85180/149‑1 (MQ=255)
                                                                                                                           taaataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGa                 <  4:976049/149‑1 (MQ=255)
                                                                                                                               taCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGa             <  3:523558/149‑1 (MQ=255)
                                                                                                                                aCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTTTCGCGACACCGGAATGAACGAc            <  4:199906/149‑1 (MQ=255)
                                                                                                                                aCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGAc            >  4:417744/1‑149 (MQ=255)
                                                                                                                                  aaaaaaTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGc          >  1:151126/1‑149 (MQ=255)
                                                                                                                                  aaaaaaTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGc          >  3:179697/1‑149 (MQ=255)
                                                                                                                                    aaaaTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCg        >  4:681111/1‑149 (MQ=255)
                                                                                                                                      aaTGGGACGGTGCGTGCCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCCGTGGCGCGCCAACGGAATGACCGACCCCGAt      >  4:600444/1‑149 (MQ=255)
                                                                                                                                      aaTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGAt      >  4:490977/1‑149 (MQ=255)
                                                                                                                                          ggACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCg  <  2:603233/149‑1 (MQ=255)
                                                                                                                                          ggACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCg  <  3:1024784/149‑1 (MQ=255)
                                                                                                                                          ggACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGCTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCg  <  4:112380/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                              
GACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCG  >  NC_000913/3953288‑3953574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: