Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,353,172 C→G 5.5% intergenic (+91/+28) ghoT → / ← lysU toxin of GhoTS toxin‑antitoxin pair; membrane‑lytic protein; stimulator of persister cell formation/lysine tRNA synthetase, inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,353,1720CG 5.5% 499.7 / 10.9 141intergenic (+91/+28)ghoT/lysUtoxin of GhoTS toxin‑antitoxin pair; membrane‑lytic protein; stimulator of persister cell formation/lysine tRNA synthetase, inducible
Reads supporting (aligned to +/- strand):  ref base C (59/74);  new base G (4/4);  total (63/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

GTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATAT  >  NC_000913/4353025‑4353320
                                                                                                                                                   |                                                                                                                                                    
gTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCt                                                                                                                                                     <  4:618841/149‑1 (MQ=255)
 tCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGATGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTg                                                                                                                                                    <  1:9440/149‑1 (MQ=255)
  cGGAATATCATGGCCAAGGAGTCTGCTTGTGGCCTTTCTTTTTTCGCTCTTTTAGGATCTGTACTTGAGTGTACTCGGTGGTTATCGTGAAACGAAGCGTCTCTCAGCAAAAGTGGATTTGCGGTACGATGAACGATGCAGTCGCGTGa                                                                                                                                                   <  4:975622/149‑1 (MQ=255)
  cGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGa                                                                                                                                                   <  4:610112/149‑1 (MQ=255)
    gAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACt                                                                                                                                                 >  2:118057/1‑149 (MQ=255)
    gAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACt                                                                                                                                                 >  2:68691/1‑149 (MQ=255)
    gAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACt                                                                                                                                                 >  1:109104/1‑149 (MQ=255)
     aaTAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAATTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTg                                                                                                                                                >  3:607691/1‑149 (MQ=255)
        aaCCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCAGGCGACTGCtt                                                                                                                                             <  3:1033597/149‑1 (MQ=255)
         aCCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTc                                                                                                                                            <  2:11434/149‑1 (MQ=255)
         aCCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTc                                                                                                                                            <  3:123739/149‑1 (MQ=255)
         aCCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTc                                                                                                                                            <  1:761673/149‑1 (MQ=255)
           cTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGTACGATGAACGAAGCAGTCGCCTGACTGCTTCGt                                                                                                                                          >  3:700810/1‑149 (MQ=255)
           cTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGt                                                                                                                                          >  2:123801/1‑149 (MQ=255)
            tGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGtt                                                                                                                                         <  1:719064/149‑1 (MQ=255)
            tGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGtt                                                                                                                                         <  4:1026184/149‑1 (MQ=255)
            tGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGtt                                                                                                                                         >  4:786068/1‑149 (MQ=255)
             ggCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTc                                                                                                                                        >  3:639573/1‑149 (MQ=255)
               ccAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCAGGCGACTGCTTCGTTCAt                                                                                                                                      <  3:901321/149‑1 (MQ=255)
                cAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGTGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAATCGTCTCTCCTCAAAACTGGATTTGCGGAACGACGAACGAAGCAGTCGCCTGACTGTTTCGTTCAtt                                                                                                                                     <  4:683688/149‑1 (MQ=255)
                cAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTAGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCAtt                                                                                                                                     >  1:644429/1‑149 (MQ=255)
                   tGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTaaa                                                                                                                                  <  4:639573/149‑1 (MQ=255)
                   tGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTaaa                                                                                                                                  <  2:2491/149‑1 (MQ=255)
                     aGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGt                                                                                                                                <  3:508786/149‑1 (MQ=255)
                         tGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGaaa                                                                                                                            >  1:326380/1‑149 (MQ=255)
                            cTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCt                                                                                                                                              <  3:950872/128‑1 (MQ=255)
                              gtgGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCAtt                                                                                                                                     <  1:11578/135‑1 (MQ=255)
                                 gCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAatttatt                                                                                                                      <  3:234384/147‑1 (MQ=255)
                                 gCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAatttatt                                                                                                                      >  4:234384/1‑147 (MQ=255)
                                    ttACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTaaa                                                                                                                                  <  4:980961/132‑1 (MQ=255)
                                    ttACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTaaa                                                                                                                                  <  4:1037815/132‑1 (MQ=255)
                                    ttACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCtgt                                                                                                                 >  1:273272/1‑149 (MQ=255)
                                           tttCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCa                                                                                                          <  1:819560/149‑1 (MQ=255)
                                               tctctTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGATATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGc                                                                                                      >  1:940076/1‑149 (MQ=255)
                                               tctctTTTAGAAGCTGTAGTGGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATTGc                                                                                                      <  1:549724/149‑1 (MQ=255)
                                               tctctTTGAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGc                                                                                                      >  2:573509/1‑149 (MQ=255)
                                                   ttttAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCggg                                                                                                  >  4:585209/1‑149 (MQ=255)
                                                   ttttAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCggg                                                                                                  >  4:54671/1‑149 (MQ=255)
                                                       aGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAaga                                                                                              <  4:245577/149‑1 (MQ=255)
                                                          aagagTAGTTGAGTGGACCCGCCGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGaa                                                                                           <  3:594847/145‑1 (MQ=255)
                                                            cTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATa                                                                                         <  4:640768/149‑1 (MQ=255)
                                                             tGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATaa                                                                                        <  1:973334/149‑1 (MQ=255)
                                                              gTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAAc                                                                                       <  2:940076/149‑1 (MQ=255)
                                                              gTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAAc                                                                                       <  2:986103/149‑1 (MQ=255)
                                                                  ttGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTc                                                                                                                                            >  4:865509/1‑92 (MQ=255)
                                                                  ttGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTc                                                                                                                                            <  3:865509/92‑1 (MQ=255)
                                                                      gTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGaa                                                                               >  4:826292/1‑149 (MQ=255)
                                                                       tGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAAt                                                                              <  1:267490/149‑1 (MQ=255)
                                                                        ggACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATa                                                                             <  4:946974/149‑1 (MQ=255)
                                                                        ggACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATa                                                                             <  3:791042/149‑1 (MQ=255)
                                                                            ccGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTAt                                                                         >  2:705682/1‑149 (MQ=255)
                                                                            ccGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTAt                                                                         <  1:619284/149‑1 (MQ=255)
                                                                             cGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATg                                                                        <  3:622189/149‑1 (MQ=255)
                                                                              gCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAACAACGTCGCGAATAGTATgg                                                                       <  3:826292/149‑1 (MQ=255)
                                                                                  gTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGt                                                                   >  4:7291/1‑149 (MQ=255)
                                                                                  gTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGt                                                                   <  2:109104/149‑1 (MQ=255)
                                                                                      tCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGt                                                               <  1:705682/149‑1 (MQ=255)
                                                                                        gTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTaa                                                             >  3:382997/1‑149 (MQ=255)
                                                                                         tGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTa                                                                 >  1:340204/1‑144 (MQ=255)
                                                                                         tGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTa                                                                 <  2:340204/144‑1 (MQ=255)
                                                                                         tGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTaaa                                                            >  4:132806/1‑149 (MQ=255)
                                                                                         tGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCCGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTaaa                                                            <  4:607691/149‑1 (MQ=255)
                                                                                          gAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAAc                                                           >  4:619094/1‑149 (MQ=255)
                                                                                           aaaCGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGACCGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACa                                                          <  3:250767/149‑1 (MQ=255)
                                                                                                aaGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATa                                                     >  3:149992/1‑149 (MQ=255)
                                                                                                     tctctcCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGt                                                                   >  1:863728/1‑130 (MQ=255)
                                                                                                     tctctcCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGt                                                                   <  2:863728/130‑1 (MQ=255)
                                                                                                     tctctcCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCAt                                                >  1:329068/1‑149 (MQ=255)
                                                                                                       tctcCTCAAAACTGGATTTGCGGAACGATGAACTAAGCAGTCGCCTAACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTc                                              <  4:696191/149‑1 (MQ=255)
                                                                                                       tctcCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTc                                              >  3:4232/1‑149 (MQ=255)
                                                                                                       tctcCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTc                                              <  4:19375/149‑1 (MQ=255)
                                                                                                         tccacAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCgg                                            >  4:892703/1‑149 (MQ=255)
                                                                                                           ctcAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTc                                          <  3:132806/149‑1 (MQ=255)
                                                                                                           ctcAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTc                                          >  1:623310/1‑149 (MQ=255)
                                                                                                           ctcAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGCAAACAGCATAATCATTCGGTc                                          >  3:497118/1‑149 (MQ=255)
                                                                                                            tcAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCg                                         >  2:1035574/1‑149 (MQ=255)
                                                                                                            tcAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCg                                         >  1:688650/1‑149 (MQ=255)
                                                                                                             cAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGa                                        <  4:675758/149‑1 (MQ=255)
                                                                                                                aaCTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAc                                     >  4:770211/1‑149 (MQ=255)
                                                                                                                  cTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCa                                   <  1:980820/149‑1 (MQ=255)
                                                                                                                      aTTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATAc                               >  2:338068/1‑149 (MQ=255)
                                                                                                                            ggAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGAc                         >  3:293465/1‑149 (MQ=255)
                                                                                                                                 gATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCg                    <  3:620209/149‑1 (MQ=255)
                                                                                                                                 gATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCg                    >  1:467179/1‑149 (MQ=255)
                                                                                                                                    gAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTa                                                                          <  2:689546/92‑1 (MQ=255)
                                                                                                                                    gAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGtt                 <  2:850183/149‑1 (MQ=255)
                                                                                                                                    gAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTGCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTa                                                                          >  1:689546/1‑92 (MQ=255)
                                                                                                                                     aaCGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTg                >  3:802090/1‑149 (MQ=255)
                                                                                                                                     aaCGAAGCAGTCAGGCGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTg                >  4:745215/1‑149 (MQ=255)
                                                                                                                                      aCGAAGCAGTCAGGCGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTgg               >  4:469182/1‑149 (MQ=255)
                                                                                                                                       cGAAGCAGTCAGGCGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTggc              >  3:108549/1‑149 (MQ=255)
                                                                                                                                       cGAAGCAGTCAGGCGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTggc              >  2:757808/1‑149 (MQ=255)
                                                                                                                                        gAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCg                                             <  1:286594/117‑1 (MQ=255)
                                                                                                                                        gAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCg                                             >  2:286594/1‑117 (MQ=255)
                                                                                                                                          aGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTggcggc           <  1:118057/149‑1 (MQ=255)
                                                                                                                                             aGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGa        >  4:228158/1‑149 (MQ=255)
                                                                                                                                              gTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGAc       <  2:612826/149‑1 (MQ=255)
                                                                                                                                                   cTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCatat  >  3:690932/1‑149 (MQ=255)
                                                                                                                                                   cTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCatat  <  4:493400/149‑1 (MQ=255)
                                                                                                                                                   cTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCatat  <  1:860253/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATAT  >  NC_000913/4353025‑4353320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: