Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,488,425 A→T 7.0% intergenic (+25/+136) lptG → / ← yjgR lipopolysaccharide export ABC permease/DUF853 family protein with NTPase fold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,488,4250AT 7.0% 435.2 / 18.0 130intergenic (+25/+136)lptG/yjgRlipopolysaccharide export ABC permease/DUF853 family protein with NTPase fold
Reads supporting (aligned to +/- strand):  ref base A (62/58);  new base T (5/4);  total (68/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

Only 100 of 131 total aligned reads displayed.

TACTGGACCAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCT  >  NC_000913/4488279‑4488572
                                                                                                                                                  |                                                                                                                                                   
tACTGGACCAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCGCTGTTGCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAg                                                                                                                                                  <  4:665296/149‑1 (MQ=255)
tACTGGACCAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGaaa                                                                                                                                                   <  2:826684/149‑1 (MQ=255)
   tGGACCAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCt                                                                                                                                                >  1:26411/1‑149 (MQ=255)
         aGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCttt                                                                                                                                          <  3:430946/149‑1 (MQ=255)
          gATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTtta                                                                                                                                         >  2:767640/1‑149 (MQ=255)
           aTCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCTTTCGCTCCCTttat                                                                                                                                        <  1:624799/149‑1 (MQ=255)
           aTCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCTTTCGCTCCCTttat                                                                                                                                        <  4:822728/149‑1 (MQ=255)
           aTCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTttat                                                                                                                                        <  1:68979/149‑1 (MQ=255)
           aTCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTttat                                                                                                                                        >  3:300687/1‑149 (MQ=255)
             cTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCTTTCGCTCCCTttattc                                                                                                                                      <  1:628674/149‑2 (MQ=255)
             cTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTttatta                                                                                                                                      >  4:287106/1‑149 (MQ=255)
                  gCCCTCTGACGTTTGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGtt                                                                                                                                 <  3:369773/149‑1 (MQ=255)
                   cccGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTa                                                                                                                                <  3:124170/149‑1 (MQ=255)
                   cccGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTa                                                                                                                                <  2:91671/149‑1 (MQ=255)
                      gCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCa                                                                                                                             >  2:343038/1‑149 (MQ=255)
                        tGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCaaa                                                                                                                           <  3:363315/149‑1 (MQ=255)
                            gTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATTGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAgtgt                                                                                                                       >  1:130651/1‑149 (MQ=255)
                               ggTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTCCTTCTTAATCAGCCTGTGGCTGTTAATGAGACAATCGTAACCGAATGAACGAATAAAGGGAGCGCAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCt                                                                                                                    >  4:517627/1‑149 (MQ=255)
                                 tttATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCTTTCGCTCCCTttat                                                                                                                                        >  4:281427/1‑127 (MQ=255)
                                     tGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTc                                                                                                              >  2:462508/1‑149 (MQ=255)
                                     tGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAAGAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTc                                                                                                              >  2:879746/1‑149 (MQ=255)
                                      ggCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCa                                                                                                             >  3:771757/1‑149 (MQ=255)
                                       gCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCAt                                                                                                            >  4:816329/1‑149 (MQ=255)
                                         aTCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTc                                                                                                          <  2:139179/149‑1 (MQ=255)
                                         aTCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTc                                                                                                          >  2:325443/1‑149 (MQ=255)
                                          tCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTcc                                                                                                         <  4:300687/149‑1 (MQ=255)
                                          tCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTcc                                                                                                         >  4:342437/1‑149 (MQ=255)
                                           tgcgccgATGATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCt                                                                                                        >  3:767915/3‑149 (MQ=255)
                                                 gATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGaaaaa                                                                                                  <  1:462508/149‑1 (MQ=255)
                                                 gATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGaaaaa                                                                                                  <  3:947660/149‑1 (MQ=255)
                                                   tcatcGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAaata                                                                                                >  1:510983/1‑149 (MQ=255)
                                                    catcGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAACTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAaataa                                                                                               <  4:578159/149‑1 (MQ=255)
                                                     atcGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAaataat                                                                                              >  4:445316/1‑149 (MQ=255)
                                                      tcGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTc                                                                                                          >  2:119519/1‑136 (MQ=255)
                                                      tcGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTc                                                                                                          <  1:119519/136‑1 (MQ=255)
                                                      tcGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAAtt                                                                                             <  1:731093/149‑1 (MQ=255)
                                                         gcgcACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTaaa                                                                                          >  3:284248/1‑149 (MQ=255)
                                                          cgcACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTaaaa                                                                                         <  4:265499/149‑1 (MQ=255)
                                                               tattGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCaa                                                                                    <  4:641874/147‑1 (MQ=255)
                                                               tGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTc                                                                                                                  <  2:633253/119‑1 (MQ=255)
                                                               tGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTc                                                                                                                  >  1:633253/1‑119 (MQ=255)
                                                               tGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGCTCATTCCTGAAAAATAATTAAAATTCaa                                                                                    <  1:936582/149‑1 (MQ=255)
                                                                 ttGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTaa                                                                                           >  3:451396/1‑140 (MQ=255)
                                                                 ttGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTaa                                                                                           <  4:451396/140‑1 (MQ=255)
                                                                 ttGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAata                                                                                  <  1:518681/149‑1 (MQ=255)
                                                                   gCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAatata                                                                                <  2:363154/149‑1 (MQ=255)
                                                                     cAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTc                                                                                                                  <  2:500210/113‑1 (MQ=255)
                                                                     cAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTc                                                                                                                  >  1:500210/1‑113 (MQ=255)
                                                                     cAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCAt                                                                                                            >  4:541527/1‑119 (MQ=255)
                                                                     cAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCAt                                                                                                            <  3:541527/119‑1 (MQ=255)
                                                                      aaGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTg                                                                             >  2:743356/1‑149 (MQ=255)
                                                                       aGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCaa                                                                                    <  1:846121/141‑1 (MQ=255)
                                                                       aGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCaa                                                                                    >  2:846121/1‑141 (MQ=255)
                                                                        gcgcCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCa                                                                           <  2:510983/149‑1 (MQ=255)
                                                                                ttcttcttAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAgtggggtg                                                                   >  3:244526/1‑149 (MQ=255)
                                                                                 tcttcttAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAgtggggtgg                                                                  >  4:388688/1‑149 (MQ=255)
                                                                                     cttAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAgtggggtg                                                                   >  2:915454/1‑144 (MQ=255)
                                                                                     cttAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCAt                                                              >  1:319566/1‑149 (MQ=255)
                                                                                     cttAATCAGCCTGTGGCCGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGTAgtggggtg                                                                   <  1:915454/144‑1 (MQ=255)
                                                                                      ttAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATg                                                             >  3:865826/1‑149 (MQ=255)
                                                                                        aaTCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGAt                                                           >  4:718441/1‑149 (MQ=255)
                                                                                         aTCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATa                                                          >  3:380236/1‑149 (MQ=255)
                                                                                          tCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATaa                                                         <  3:816329/149‑1 (MQ=255)
                                                                                                      tGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCg                                             >  2:182918/1‑149 (MQ=255)
                                                                                                      tGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCg                                             >  1:989582/1‑149 (MQ=255)
                                                                                                            tGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCAt                                       >  2:242044/1‑149 (MQ=255)
                                                                                                             gagaAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATc                                      <  1:182918/149‑1 (MQ=255)
                                                                                                               gaAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCa                                     >  1:471341/1‑148 (MQ=255)
                                                                                                               gaAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCa                                     <  2:471341/148‑1 (MQ=255)
                                                                                                               gaAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAg                                    >  2:634289/1‑149 (MQ=255)
                                                                                                                aaaaTCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAgg                                   >  2:329970/1‑149 (MQ=255)
                                                                                                                    tCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCGGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCaaa                               <  4:767915/149‑1 (MQ=255)
                                                                                                                    tCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCaaa                               <  2:995651/149‑1 (MQ=255)
                                                                                                                      gTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGa                             <  4:253232/149‑1 (MQ=255)
                                                                                                                         aCCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTGCGTTCATTACTGAAAAATAATTAAAATTCGATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACaa                          <  1:64900/149‑1 (MQ=255)
                                                                                                                          ccGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACaaa                         >  1:831683/1‑149 (MQ=255)
                                                                                                                           cGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAAt                        <  3:474999/149‑1 (MQ=255)
                                                                                                                           cGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAAt                        >  2:615209/1‑149 (MQ=255)
                                                                                                                           agAATGAAAGAATAAGGGGATCGAAAGCTCAATTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAACATTCAATATATAGCAAAGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAAt                        <  2:609985/148‑1 (MQ=255)
                                                                                                                            gAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCaa                                                                                    <  3:174866/88‑1 (MQ=255)
                                                                                                                               tGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAgtggg                                                                      >  3:301050/1‑99 (MQ=255)
                                                                                                                               tGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAgtggg                                                                      <  4:301050/99‑1 (MQ=255)
                                                                                                                                gAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGc                   <  1:767640/149‑1 (MQ=255)
                                                                                                                                gAACGAATAAAGAGAGCGAAAGCTCCCTTTAGTATTGTTAGAAAAGTGTGCTTCGTTCATTCATGAAAAATCATTAAAATTGATAATTTTGCAGTGGCGTGCGCATGATAAGACGCGCCAGCGTTGCATCAGACAAATACAAATCTCGc                   <  2:758668/149‑1 (MQ=255)
                                                                                                                                  aataaTAAAGGGAGCTTTCGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAg                                    >  4:547189/4‑130 (MQ=255)
                                                                                                                                  aataaTAAAGGGAGCTTTCGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAg                                    <  3:547189/127‑1 (MQ=255)
                                                                                                                                  aataaTAAAGGGAGCTTTCGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCt                 >  2:501967/4‑149 (MQ=255)
                                                                                                                                      aTAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACt             >  2:303195/1‑149 (MQ=255)
                                                                                                                                       tAAAGGGAGCTTTCGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACtt            >  1:195215/1‑149 (MQ=255)
                                                                                                                                       tAAAGGGAGCTTTCGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACtt            >  3:536862/1‑149 (MQ=255)
                                                                                                                                       tAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACtt            >  2:318558/1‑149 (MQ=255)
                                                                                                                                         aaGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTAc          >  3:641279/1‑149 (MQ=255)
                                                                                                                                            ggAGCGAAAGCTCCCTTTATTATTGTTAGCAATGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACctt       >  4:898321/1‑149 (MQ=255)
                                                                                                                                            ggAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACctt       <  4:771757/149‑1 (MQ=255)
                                                                                                                                             gAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACcttc      <  2:831683/149‑1 (MQ=255)
                                                                                                                                              aGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACcttct     >  1:276010/1‑149 (MQ=255)
                                                                                                                                               gCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACcttctt    <  1:343038/149‑1 (MQ=255)
                                                                                                                                                cGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCttcttc   <  2:397171/149‑1 (MQ=255)
                                                                                                                                                cGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCttcttc   <  1:858987/149‑1 (MQ=255)
                                                                                                                                                 gAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTGCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCTAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTtcttct  <  3:718441/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TACTGGACCAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCT  >  NC_000913/4488279‑4488572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: