Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,614,633 T→C 9.8% intergenic (+373/+47) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,614,6330TC 9.8% 197.5 / 9.6 70intergenic (+373/+47)yjjV/yjjWputative DNase/putative pyruvate formate lyase activating enzyme
Reads supporting (aligned to +/- strand):  ref base T (28/35);  new base C (4/3);  total (32/38)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

CATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGTGGCGCTCGCCCAGCT  >  NC_000913/4614485‑4614778
                                                                                                                                                    |                                                                                                                                                 
cATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCtt                                                                                                                                                   <  2:401169/149‑1 (MQ=255)
 aTCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTa                                                                                                                                                  <  2:404820/149‑1 (MQ=255)
      gCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAgg                                                                                                                                             <  2:653300/149‑1 (MQ=255)
       cAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGc                                                                                                                                            >  4:210958/1‑149 (MQ=255)
        aGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGGTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCACGcc                                                                                                                                           <  1:918038/149‑1 (MQ=255)
            ggCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATAAGACGCGTCAAGCGTCGCATCAGGcattgt                                                                                                                                       <  4:898262/149‑6 (MQ=14)
             gcgcATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACaa                                                                                                                                      <  2:36076/149‑1 (MQ=255)
              cgcATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAg                                                                                                                                     <  1:912696/149‑1 (MQ=255)
               gcATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                                                                              <  3:895357/139‑1 (MQ=255)
               gcATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                                                                              >  4:895357/1‑139 (MQ=255)
               gcATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGt                                                                                                                                    <  4:205529/149‑1 (MQ=255)
                 aTAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCt                                                                                                                                  >  1:104180/1‑149 (MQ=255)
                     tGCCTGATGCGACGCTTATCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGc                                                                                                                              <  4:112472/149‑1 (MQ=37)
                              cGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTa                                                                                                                     <  4:5336/149‑1 (MQ=255)
                              cGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTa                                                                                                                     <  2:615235/149‑1 (MQ=255)
                               gACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAg                                                                                                                    >  4:486323/1‑149 (MQ=255)
                                aCGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAgg                                                                                                                   >  2:67315/1‑149 (MQ=255)
                                  gCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTGTGTGCCGAACCGTAGGcc                                                                                                                 >  2:445250/1‑149 (MQ=255)
                                  gCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGcc                                                                                                                 >  2:506735/1‑149 (MQ=255)
                                         gcgTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCg                                                                                                          >  1:710115/1‑149 (MQ=255)
                                            tCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCa                                                                                                       <  4:867720/149‑1 (MQ=255)
                                                 tCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTATGTGCCGAACCGTAGGCCGTATCCGGCATGTca                                                                                                  >  4:71693/1‑149 (MQ=255)
                                                  cATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTcac                                                                                                 <  3:839/149‑1 (MQ=255)
                                                     gCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACaaa                                                                                              >  2:1012069/1‑149 (MQ=255)
                                                     gCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACaaa                                                                                              >  1:571692/1‑149 (MQ=255)
                                                       cTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATa                                                                                            <  1:733544/149‑1 (MQ=255)
                                                              tttGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGcc                                                                                     >  4:654500/1‑149 (MQ=255)
                                                              tttGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGcc                                                                                     <  1:222711/149‑1 (MQ=255)
                                                              tttGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGcc                                                                                     >  3:83167/1‑149 (MQ=255)
                                                                tgtgCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCgg                                                                                   <  2:22475/149‑1 (MQ=255)
                                                                       aaCCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATc                                                                            >  2:484934/1‑149 (MQ=255)
                                                                          cGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAAc                                                                         <  4:99361/149‑1 (MQ=255)
                                                                          cGTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAAc                                                                         <  1:679034/149‑1 (MQ=255)
                                                                              ggCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTCATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGc                                                                     <  4:755785/149‑1 (MQ=255)
                                                                                ccGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATc                                                                                                                                                <  3:446082/72‑1 (MQ=255)
                                                                                ccGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATcc                                                                                                           <  4:138893/109‑1 (MQ=255)
                                                                                ccGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATcc                                                                                                           >  3:138893/1‑109 (MQ=255)
                                                                                    aTAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCAccc                                                               >  4:47486/1‑149 (MQ=255)
                                                                                     tAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCAcccc                                                              <  3:244350/149‑1 (MQ=255)
                                                                                          cGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCa                                                         <  2:942138/149‑1 (MQ=255)
                                                                                               gcgcCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCttt                                                    <  1:484934/149‑1 (MQ=255)
                                                                                               gcgcCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCttt                                                    <  3:503080/149‑1 (MQ=255)
                                                                                               acgccgcATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGcc                                                                                                                 <  2:793378/87‑1 (MQ=37)
                                                                                                 gccgcATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTaa                                                  >  1:651236/1‑149 (MQ=255)
                                                                                                 gccacATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTaa                                                  <  3:73119/145‑1 (MQ=255)
                                                                                                  ccgcATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAAc                                                 <  3:210958/149‑1 (MQ=255)
                                                                                                  ccgcATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAAc                                                 <  3:486323/149‑1 (MQ=255)
                                                                                                           gCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCATAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGcc                                        <  1:47341/149‑1 (MQ=255)
                                                                                                                    gcACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCa                               >  2:407467/1‑149 (MQ=255)
                                                                                                                      acaATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAAc                             <  4:413882/149‑1 (MQ=255)
                                                                                                                      acaATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAAc                             >  1:83480/1‑149 (MQ=255)
                                                                                                                        aaTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGt                           >  4:998682/1‑149 (MQ=255)
                                                                                                                        aaTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGCCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGt                           >  1:101600/1‑149 (MQ=255)
                                                                                                                           gCCTGATAAGACGCGTCAAGCGTCGCATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCAcccc                                                              <  3:487620/111‑1 (MQ=37)
                                                                                                                           gCCTGATAAGACGCGTCAAGCGTCGCATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCAcccc                                                              >  4:487620/1‑111 (MQ=37)
                                                                                                                           gCCTGATAAGACGCGTCAAGCGTCGCATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGAGCACCTTTAACGCATCAGCCAACGGCTCAACGTCtt                        >  4:712494/1‑149 (MQ=255)
                                                                                                                            ccTGATAAGACGCGTCAAGCGTCGCATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTc                       >  4:213658/1‑149 (MQ=255)
                                                                                                                             cTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTcc                      <  2:571692/149‑1 (MQ=255)
                                                                                                                             cTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTcc                      <  1:506735/149‑1 (MQ=255)
                                                                                                                             cTGATAAGACGCGTCAAGCGTCGCATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTcc                      >  3:509640/1‑149 (MQ=255)
                                                                                                                               gATGCGACGCTTGACGCGGATTATCAGGCCTACAACTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCgg                    >  2:44679/1‑149 (MQ=255)
                                                                                                                                  gCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCGACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGt                 <  2:104180/149‑1 (MQ=255)
                                                                                                                                  gCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGt                 <  1:984255/149‑1 (MQ=255)
                                                                                                                                    gACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGTgg               >  4:54052/1‑149 (MQ=255)
                                                                                                                                         ttGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGTGGCGCTc          >  2:693174/1‑149 (MQ=255)
                                                                                                                                            aCGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGTGGCGCTCGcc       >  4:746899/1‑149 (MQ=255)
                                                                                                                                             cgcgTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGTGGCGCTCGccc      <  1:514525/149‑1 (MQ=255)
                                                                                                                                              gcgTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGTGGCGCTCGCCCa     >  1:957381/1‑149 (MQ=255)
                                                                                                                                              gcgTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGTGGCGCTCGCCCa     >  1:682055/1‑149 (MQ=255)
                                                                                                                                                 tCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGTGGCGCTCGCCCAGCt  >  2:134613/1‑149 (MQ=255)
                                                                                                                                                 tCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGTGGCGCTCGCCCAGCt  <  1:471017/149‑1 (MQ=255)
                                                                                                                                                   ttATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAAc                                                                         >  2:856216/1‑76 (MQ=255)
                                                                                                                                                   ttATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAAc                                                                         <  1:856216/76‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGTGGCGCTCGCCCAGCT  >  NC_000913/4614485‑4614778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: