Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,636,212 T→A 5.3% L69* (TTA→TAA)  creB → response regulator in two‑component regulatory system with CreC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,636,2120TA 5.3% 514.1 / 14.8 151L69* (TTA→TAA) creBresponse regulator in two‑component regulatory system with CreC
Reads supporting (aligned to +/- strand):  ref base T (74/69);  new base A (3/5);  total (77/74)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.53e-01

Only 100 of 151 total aligned reads displayed.

TCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCT  >  NC_000913/4636065‑4636358
                                                                                                                                                   |                                                                                                                                                  
tCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTa                                                                                                                                                   >  1:334931/1‑149 (MQ=255)
 cTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTAc                                                                                                                                                  <  2:33231/149‑1 (MQ=255)
  tACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGCCGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACt                                                                                                                                                 <  1:470603/149‑1 (MQ=255)
  tACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACt                                                                                                                                                 <  2:268883/149‑1 (MQ=255)
    cATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTgg                                                                                                                                               >  2:252564/1‑149 (MQ=255)
    cATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTg                                                                                                                                                >  1:628773/1‑148 (MQ=255)
       gTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTAACTGgcgg                                                                                                                                            >  1:427088/1‑148 (MQ=255)
        ttGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCt                                                                                                                                           >  4:414259/1‑149 (MQ=255)
          gcagcaGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTcc                                                                                                                                         >  4:802243/1‑149 (MQ=255)
            agcagGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTAACTGgcggcacaa                                                                                                                                       <  3:1035404/149‑7 (MQ=255)
             gcagGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTAACTGgcggcacaat                                                                                                                                      <  2:427088/149‑8 (MQ=255)
             gcagGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTAACTGgcggcacaat                                                                                                                                      <  4:323144/149‑8 (MQ=255)
             gcagGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTAACTGgcggcacaat                                                                                                                                      <  4:555897/149‑8 (MQ=255)
                ggAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCgg                                                                                                                                   <  1:1024028/149‑1 (MQ=255)
                ggAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCgg                                                                                                                                   >  1:518726/1‑149 (MQ=255)
                 gAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGc                                                                                                                                  >  3:380505/1‑149 (MQ=255)
                 gAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGc                                                                                                                                  >  4:829746/1‑149 (MQ=255)
                     gTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTa                                                                                                                              >  4:93492/1‑149 (MQ=255)
                      ttttGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTAc                                                                                                                             >  1:1062267/1‑149 (MQ=255)
                           ccGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTa                                                                                                                        <  3:620047/149‑1 (MQ=255)
                            cGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTAc                                                                                                                       >  1:714180/1‑149 (MQ=255)
                               cGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGt                                                                                                                    <  4:949556/149‑1 (MQ=255)
                                  ggTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTcc                                                                                                                 >  3:376341/1‑149 (MQ=255)
                                   gTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCt                                                                                                                >  2:320865/1‑149 (MQ=255)
                                    tCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTg                                                                                                               >  4:720568/1‑149 (MQ=255)
                                      tttGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGAc                                                                                                             >  4:403258/1‑149 (MQ=255)
                                      tttGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGAc                                                                                                             >  2:93117/1‑149 (MQ=255)
                                      tttGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGAc                                                                                                             >  1:356169/1‑149 (MQ=255)
                                         gAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGc                                                                                                          <  3:507334/149‑1 (MQ=255)
                                               ggCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAg                                                                                                    <  3:1036133/149‑1 (MQ=255)
                                                   tGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGaa                                                                                                <  1:93117/149‑1 (MQ=255)
                                                   tGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGaa                                                                                                <  4:678293/149‑1 (MQ=255)
                                                      cGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAagag                                                                                             <  3:593082/149‑1 (MQ=255)
                                                            tGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATcc                                                                                                                                     <  4:572954/103‑1 (MQ=255)
                                                            tGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATcc                                                                                                                                     >  3:572954/1‑103 (MQ=255)
                                                             ggATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTAc                                                                                                                       <  3:563111/116‑1 (MQ=255)
                                                              gATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCg                                                                                     >  3:339756/1‑149 (MQ=255)
                                                              gATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCg                                                                                     >  4:202113/1‑149 (MQ=255)
                                                              gATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGCCCCGAAGTGAAGAGGTCGATCg                                                                                     >  1:1059939/1‑149 (MQ=255)
                                                               aTAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGc                                                                                    >  2:270722/1‑149 (MQ=255)
                                                                tAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGcc                                                                                   <  1:320865/149‑1 (MQ=255)
                                                                tAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGcc                                                                                   <  2:1059939/149‑1 (MQ=255)
                                                                 aaaGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCt                                                                                  >  2:336838/1‑149 (MQ=255)
                                                                    gCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGGTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCt                                                                               >  1:133670/1‑149 (MQ=255)
                                                                      tCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTa                                                                                                                        <  4:500961/106‑1 (MQ=255)
                                                                      tCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAaga                                                                                              <  3:791814/132‑1 (MQ=255)
                                                                      tCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTg                                                                             >  2:918092/1‑149 (MQ=255)
                                                                       cGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTgg                                                                            <  4:841710/149‑1 (MQ=255)
                                                                       cGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTgg                                                                            <  2:334931/149‑1 (MQ=255)
                                                                        gCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTggg                                                                           <  3:532597/149‑1 (MQ=255)
                                                                         cAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGc                                                                          <  4:376341/149‑1 (MQ=255)
                                                                          aaGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCt                                                                         >  2:387748/1‑149 (MQ=255)
                                                                                 tACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAAtt                                                                  <  1:546379/149‑1 (MQ=255)
                                                                                  aCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAGGTGAAGAGGTCGAt                                                                                       <  4:114127/127‑1 (MQ=255)
                                                                                  aCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAGGTGAAGAGGTCGAt                                                                                       >  3:114127/1‑127 (MQ=255)
                                                                                  aCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGATACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTg                                                                 <  2:696535/149‑1 (MQ=255)
                                                                                    ccGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGt                                                               >  4:221318/1‑149 (MQ=255)
                                                                                    ccGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGt                                                               >  4:209347/1‑149 (MQ=255)
                                                                                    ccGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGt                                                               <  1:1057261/149‑1 (MQ=255)
                                                                                     cGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTg                                                              <  1:274226/149‑1 (MQ=255)
                                                                                         gTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTgg                                                                <  4:72354/143‑1 (MQ=255)
                                                                                         gTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTga                                                          >  3:495853/1‑149 (MQ=255)
                                                                                         gTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTga                                                          >  4:345827/1‑149 (MQ=255)
                                                                                         gTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTACCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTga                                                          <  2:518726/149‑1 (MQ=255)
                                                                                          tCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAaga                                                                                              <  2:710809/112‑1 (MQ=255)
                                                                                          tCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAaga                                                                                              >  1:710809/1‑112 (MQ=255)
                                                                                          tCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTgac                                                         <  2:209265/149‑1 (MQ=255)
                                                                                          tCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTgac                                                         >  1:25371/1‑149 (MQ=255)
                                                                                            aTGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTgacga                                                       >  4:840698/1‑149 (MQ=255)
                                                                                            aTGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTgacga                                                       <  4:970706/149‑1 (MQ=255)
                                                                                            aTGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTgacga                                                       >  3:317488/1‑149 (MQ=255)
                                                                                             tGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTgacgac                                                      >  1:438551/1‑149 (MQ=255)
                                                                                                  ctcGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGt                                                 >  4:282526/1‑149 (MQ=255)
                                                                                                  ctcGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGt                                                 >  1:814272/1‑149 (MQ=255)
                                                                                                  ctcGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGt                                                 <  3:202113/149‑1 (MQ=255)
                                                                                                    cGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTgg                                               <  1:765047/149‑1 (MQ=255)
                                                                                                     gATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGc                                              <  3:829746/149‑1 (MQ=255)
                                                                                                      aTGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCt                                             >  4:833719/1‑149 (MQ=255)
                                                                                                             tCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGt                                      <  4:1009418/149‑1 (MQ=255)
                                                                                                              cTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTTCTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTCGTGCTGACGACTACGTGGCTAAACCGtt                                     <  3:899814/149‑1 (MQ=255)
                                                                                                                 ccGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTc                                  <  4:59313/149‑1 (MQ=255)
                                                                                                                   ggATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTg                                                                             >  3:797122/1‑104 (MQ=255)
                                                                                                                   ggATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTg                                                                             <  4:797122/104‑1 (MQ=255)
                                                                                                                   ggATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTgacgac                                                      >  3:300276/1‑127 (MQ=255)
                                                                                                                      tatTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCAcccc                             >  1:691392/1‑149 (MQ=255)
                                                                                                                        ttAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCcgc                           >  3:122186/1‑149 (MQ=255)
                                                                                                                        ttAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCcgc                           >  4:618100/1‑149 (MQ=255)
                                                                                                                        ttAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCcgc                           >  2:866534/1‑149 (MQ=255)
                                                                                                                        ttAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCcgc                           >  4:711318/1‑149 (MQ=255)
                                                                                                                        ttAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCACGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCcgc                           >  4:568773/1‑149 (MQ=255)
                                                                                                                           gCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGaa                        <  3:209347/149‑1 (MQ=255)
                                                                                                                               cTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAgtgt                    <  4:312485/149‑1 (MQ=255)
                                                                                                                                tttGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTg                                                                 <  3:453643/103‑1 (MQ=255)
                                                                                                                                tttGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTg                                                              <  1:206295/106‑1 (MQ=255)
                                                                                                                                tttGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGtgtg                   >  1:455708/1‑149 (MQ=255)
                                                                                                                                 ttGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTgc                  <  3:92687/149‑1 (MQ=255)
                                                                                                                                  tGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTgcg                 <  3:824360/149‑1 (MQ=255)
                                                                                                                                         tgCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGt          >  2:550753/1‑149 (MQ=255)
                                                                                                                                         tgCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGt          <  2:866484/149‑1 (MQ=255)
                                                                                                                                              ccAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCa     <  1:184645/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCT  >  NC_000913/4636065‑4636358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: