Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 700,388 A→T 5.3% L403M (TTG→ATG)  nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913700,3880AT 5.3% 388.3 / 9.8 113L403M (TTG→ATG) nagCN‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (47/60);  new base T (4/2);  total (51/62)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.76e-01

Only 100 of 116 total aligned reads displayed.

TGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCA  >  NC_000913/700241‑700536
                                                                                                                                                   |                                                                                                                                                    
tGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCaaa                                                                                                                                                     <  1:1057209/149‑1 (MQ=255)
  cAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATg                                                                                                                                                   <  3:778101/149‑1 (MQ=255)
     aaaTTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTg                                                                                                                                                <  1:1044301/149‑1 (MQ=255)
              tGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAAt                                                                                                                                       <  1:407446/149‑1 (MQ=255)
                 aCCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATAcc                                                                                                                                    <  3:707357/149‑1 (MQ=255)
                 aCCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATCGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATAcc                                                                                                                                    >  4:719577/1‑149 (MQ=255)
                   ccGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGt                                                                                                                                  >  2:528170/1‑149 (MQ=255)
                      gTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGa                                                                                                                               <  1:222346/149‑1 (MQ=255)
                           gCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATg                                                                                                                          <  3:77245/149‑1 (MQ=255)
                            cGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATgg                                                                                                                         <  1:658854/149‑1 (MQ=255)
                               cGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGgcgc                                                                                                                      >  1:951962/1‑149 (MQ=255)
                                  tATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGtt                                                                                                                   <  3:404953/149‑1 (MQ=255)
                                  tATCGTGCATCAGCACGCCGTCGATATCACAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGtt                                                                                                                   >  2:628023/1‑149 (MQ=255)
                                   aTCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGttt                                                                                                                  <  1:1008148/149‑1 (MQ=255)
                                    tCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGtttt                                                                                                                 <  3:843395/149‑1 (MQ=255)
                                       tGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTAcc                                                                                                              >  1:1031369/1‑149 (MQ=255)
                                        gCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCa                                                                                                             <  3:930677/149‑1 (MQ=255)
                                                  gCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAgc                                                                                                   <  4:598030/149‑1 (MQ=255)
                                                    cGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAgcgc                                                                                                 <  1:671981/149‑1 (MQ=255)
                                                    cGTCGATATCGCAAATTACATATTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAgcgc                                                                                                 <  4:988355/149‑1 (MQ=255)
                                                     gTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATAcc                                                                                                                                    >  1:313527/1‑113 (MQ=255)
                                                     gTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATAcc                                                                                                                                    <  2:313527/113‑1 (MQ=255)
                                                     gTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGcc                                                                                                >  4:1026421/1‑149 (MQ=255)
                                                      tCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCg                                                                                               >  4:634457/1‑149 (MQ=255)
                                                             cGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCg                                                                                        <  2:951962/149‑1 (MQ=255)
                                                              gCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTg                                                                                                                                                >  1:1049843/1‑92 (MQ=255)
                                                              gCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTg                                                                                                                                                <  2:1049843/92‑1 (MQ=255)
                                                              gCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGa                                                                                       <  2:257629/149‑1 (MQ=255)
                                                               cAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATGTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTGTTACCAGCGCAAAAGCGCCGATAGCCGAg                                                                                      >  2:404913/1‑149 (MQ=255)
                                                                aaaTTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGc                                                                                     <  3:339127/149‑1 (MQ=255)
                                                                   ttACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGt                                                                                  <  3:45417/149‑1 (MQ=255)
                                                                   ttACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGt                                                                                  <  2:89669/149‑1 (MQ=255)
                                                                   ttACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGt                                                                                  <  4:179722/149‑1 (MQ=255)
                                                                    tACGTTTTTAATGGTCATGGACTACCCAGAATTTTGACAACACTAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTg                                                                                 >  2:88568/1‑149 (MQ=255)
                                                                    tACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTg                                                                                 <  3:340104/149‑1 (MQ=255)
                                                                      cATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGAt                                                                               <  1:698137/149‑1 (MQ=255)
                                                                       aTTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATc                                                                              >  3:184092/1‑149 (MQ=255)
                                                                       aTTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATc                                                                              >  3:308546/1‑149 (MQ=255)
                                                                       aTTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATc                                                                              >  2:344035/1‑149 (MQ=255)
                                                                       aTTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATc                                                                              <  1:266489/149‑1 (MQ=255)
                                                                       aTTTTTAATGGGCATGGACTACCCAGAATATTGACGACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTGTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATc                                                                              >  2:670657/1‑149 (MQ=255)
                                                                             aaTGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTc                                                                        >  4:485472/1‑149 (MQ=255)
                                                                              aTGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCa                                                                       >  3:1017065/1‑149 (MQ=255)
                                                                                ggTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGa                                                                                                                               >  2:671782/1‑91 (MQ=255)
                                                                                ggTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGa                                                                                                                               <  1:671782/91‑1 (MQ=255)
                                                                                ggTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGa                                                                     <  1:455316/149‑1 (MQ=255)
                                                                                 gTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGaa                                                                    <  1:49489/149‑1 (MQ=255)
                                                                                 gTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGaa                                                                    <  1:100580/149‑1 (MQ=255)
                                                                                   cATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACg                                                                  <  3:35730/149‑1 (MQ=255)
                                                                                    aTGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGt                                                                 <  3:483320/149‑1 (MQ=255)
                                                                                     tGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTa                                                                <  4:439653/149‑1 (MQ=255)
                                                                                         cTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTaccac                                                            <  3:990297/149‑1 (MQ=255)
                                                                                            cccAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCgg                                                         >  1:108192/1‑149 (MQ=255)
                                                                                             ccAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTAc                                                                                                               <  2:786040/94‑1 (MQ=255)
                                                                                             ccAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTAc                                                                                                               >  1:786040/1‑94 (MQ=255)
                                                                                             ccAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGc                                                        >  3:135077/1‑149 (MQ=255)
                                                                                              cAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCa                                                       >  1:1057201/1‑149 (MQ=255)
                                                                                              cAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCa                                                       >  1:829283/1‑149 (MQ=255)
                                                                                              cAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCa                                                       >  3:152496/1‑149 (MQ=255)
                                                                                               aGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAg                                                      >  3:1013119/1‑149 (MQ=255)
                                                                                                gAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGa                                                     <  1:288167/149‑1 (MQ=255)
                                                                                                 aaTATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGAt                                                    <  2:641823/149‑1 (MQ=255)
                                                                                                     ttGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGt                                                <  3:304145/149‑1 (MQ=255)
                                                                                                      tGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTg                                               <  2:108192/149‑1 (MQ=255)
                                                                                                       gACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGc                                              >  1:844418/1‑149 (MQ=255)
                                                                                                        acaacaATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGAt                                                    >  4:606065/1‑142 (MQ=255)
                                                                                                        acaacaATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGAt                                                    <  3:606065/142‑1 (MQ=255)
                                                                                                          aacaaTAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGaa                                           >  2:662436/1‑149 (MQ=255)
                                                                                                           acaaTAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGaaa                                          <  3:226646/149‑1 (MQ=255)
                                                                                                            caaTAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAAc                                         <  2:721663/149‑1 (MQ=255)
                                                                                                             aaTAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACg                                        >  4:805510/1‑149 (MQ=255)
                                                                                                              aTAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGc                                       <  2:556634/149‑1 (MQ=255)
                                                                                                               tAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGcc                                      <  1:199257/149‑1 (MQ=255)
                                                                                                                     ccACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCGGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAgc                                >  4:565965/1‑149 (MQ=255)
                                                                                                                     ccACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAgc                                >  2:321439/1‑149 (MQ=255)
                                                                                                                         tataAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGc                                                        <  1:41832/121‑1 (MQ=255)
                                                                                                                         tataAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGc                                                        >  2:41832/1‑121 (MQ=255)
                                                                                                                         tataAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCt                            <  3:719577/149‑1 (MQ=255)
                                                                                                                         tataAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCt                            <  3:997123/149‑1 (MQ=255)
                                                                                                                           taAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTgg                          <  3:1026421/149‑1 (MQ=255)
                                                                                                                              aaGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTa                       >  3:152564/1‑149 (MQ=255)
                                                                                                                              aaGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTa                       >  2:507001/1‑149 (MQ=255)
                                                                                                                                  acaTTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGAt                                                                               >  3:885693/1‑89 (MQ=255)
                                                                                                                                  acaTTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGAt                                                                               <  4:885693/89‑1 (MQ=255)
                                                                                                                                  acaTTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTaa                   >  1:375858/1‑149 (MQ=255)
                                                                                                                                   caTTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAAt                  <  4:843820/149‑1 (MQ=255)
                                                                                                                                    aTTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATg                 >  2:866410/1‑149 (MQ=255)
                                                                                                                                    aTTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATg                 >  1:465891/1‑149 (MQ=255)
                                                                                                                                    aTTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATg                 >  1:561190/1‑149 (MQ=255)
                                                                                                                                     ttAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCg                                                                                    <  2:9761/81‑1 (MQ=255)
                                                                                                                                     ttAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCg                                                                                    >  1:9761/1‑81 (MQ=255)
                                                                                                                                     ttAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGc                >  4:840530/1‑149 (MQ=255)
                                                                                                                                         ttttCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCt            >  3:554521/1‑149 (MQ=255)
                                                                                                                                           ctCCAGCATTTGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGt                                                                 >  1:887602/2‑94 (MQ=255)
                                                                                                                                           ctCCAGCATTTGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGt                                                                 <  4:1023921/93‑1 (MQ=255)
                                                                                                                                           ctCCAGCATTTGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGt                                                                 <  2:887602/93‑1 (MQ=255)
                                                                                                                                           ctCCAGCATTTGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGt                                                                 >  3:1023921/2‑94 (MQ=255)
                                                                                                                                            tCCAGCATTTGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTc         >  4:260033/1‑149 (MQ=255)
                                                                                                                                              cAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCaa       <  1:71589/149‑1 (MQ=255)
                                                                                                                                                   aaTGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCa  <  1:662436/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCA  >  NC_000913/700241‑700536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: