Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,560,4551.G98.2% 388.8 / ‑4.8 112intergenic (‑2/+1)glpR/glpRpseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand):  ref base . (0/2);  major base G (46/62);  minor base T (2/0);  total (48/64)
Fisher's exact test for biased strand distribution p-value = 1.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 120 total aligned reads displayed.

TTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTG‑GTGACAATGCGCAAATTGCTGTGATTGAGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATC  >  NC_000913/3560178‑3560715
                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                    
ttCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTG‑GTGACAATGCGCAAATTGCTGTGATTGAGCAGT‑GCGTGCGCTACCGCTTCAGGCGTGGTGCCGATATCGATAAACCGCGTCGACCTATTGTGGCTTTGCTCCGCCACTTTGCGGGCGCTGCGCTCTTTTTCTTCGTTCTGGGTGTCCTTGCGATCGTGCACCGGCGTGTTAACCGCACTGTACTGCATTGCCACGCCGCCATGGT‑GG‑c                                                                                                                                                                                                                                                                       >  4:611150/1‑278 (MQ=255)
  cgcaccgggagagaAGGATGCGAAAATATTCTTTTACCTGCAACGTGTTAGCAACGTTGAGTTTGTTG‑GTGACAACGCTCAAATTGCGGTGATTGAGCAGG‑GCTTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGGGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑cg                                                                                                                                                                                                                                                                      <  4:235369/267‑2 (MQ=255)
    ccACCGGCAAGAATGGTGCTGTAAACTTCTTTTGCCATCAACGTGTTAGCAACGTTGAGATTGTTG‑GTGACAATTCGCGAATTGCTGTGATTGGGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑cg                                                                                                                                                                                                                                                                      <  3:272387/275‑2 (MQ=255)
     cACCGGCGGGAAGGAGTCGAAAATCTTCTTTTACCATCAACGTGTTAGGAACGTTGGGATTGTTG‑GTGACAATGCGCAAATTGCTGTGATTGAGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑cg                                                                                                                                                                                                                                                                      <  3:804654/274‑2 (MQ=255)
     cACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTG‑GTGACAATGCGCAAATTGCTGTGATTGAGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑cg                                                                                                                                                                                                                                                                      <  3:630888/274‑2 (MQ=255)
     cACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTG‑GTGACAATGCGCAAATTGCTGTGATTGAGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑cg                                                                                                                                                                                                                                                                      <  3:353219/274‑2 (MQ=255)
     cACCGGCGAGAATGATGCGAAAATCTTCTTTTACCAGCAACGTGTTGGCAACGTTGAGATTGTTG‑GTGACAATGCGCAAATTGCTGTGATTGAGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑cg                                                                                                                                                                                                                                                                      <  3:483915/274‑2 (MQ=255)
                    tgCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTG‑GTGACAATGCGCAAATTGCTGTGATTGAGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGTGCGATGCGCTCTTTTTCTTCGTCCTGGGTGGCCTTGCGCTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCTCCGCACCGCCATGAT‑GG‑CGTAGGATCAGGGTTTGc                                                                                                                                                                                                                                                      >  3:569941/1‑275 (MQ=255)
                         aaagagtcttTGACCATCAACGTGTTAGCAACGGTGAGATTGGGG‑GTGGCGATGCGTAAGGTGCTGTGATTGAGCAGG‑GCGTGCGCTCCCGCTTCCGGGGGGGGGGCGATATCGATAACCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACGTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAg                                                                                                                                                                                                                                              <  4:437888/272‑1 (MQ=255)
                         aaaTCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTG‑GTGACAATGCGCAAATTGCTGTGATTGAGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCg                                                                                                                                                                                                                                                  <  1:396804/274‑1 (MQ=255)
                          aactttcttTGACCATAAACGTGTTAGCAACGTTGAGATTGTTG‑TTGACAAGGGGCAAATTGATGTGATTGAGCAGT‑GCGGGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTGTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGcc                                                                                                                                                                                                                                                <  3:207118/271‑1 (MQ=255)
                              ttcttTTACCATCAACGTGTTAGCAACGTTGAGATTGTTG‑GTGACAATGCGCAAATTGCTGTGATTGAGCAGT‑GCGTTCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCAACGGCGTGGTAACCGAACTGGAAGGCCTCGCCGCATCGCCATGAC‑GG‑CGCAGGATCCGGTTTTGATCGGCCAGCTCa                                                                                                                                                                                                                                          >  2:570152/1‑277 (MQ=255)
                              ttcttTTACCAGCAAAGTGTTAGAAACGTTGAGATTGTTG‑GGGACAAGTCGCAAATTGCTTGGATTTAGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGTCGATATCGATAAACAGCGTCGGGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCGTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAg                                                                                                                                                                                                                                              <  3:383646/273‑1 (MQ=255)
                                     agtgtcaagggggtAGCAAGGTTGGGATGGTGG‑GTGAAGATGGGCAGATTGCGGGGGTTGAGCAGG‑GCGTGCGCTACCGCTTCCGGCGTGTTGCCGATAGCGATAAAGAGGGTCGAGCACTGGGGGGTTTACTCCGCCACGTTGCGGGCGATGCGCTCTGTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAGCCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTc                                                                                                                                                                                                                                  <  4:617885/265‑1 (MQ=255)
                                      ccATCATAGTGTTAGACACGATGAGAATGTTG‑TGTACAATGCGAAAATTGCTGTGATTTAGTAGG‑GCGTGCGCTACCGCTTCCGGCGAGGTGCGAATATCGATAGACTGCTTCGGGCCATTGGGGATTTGCTCCACCACTAGGCGGGCGAAGCGCTCGTTGTCTTGGTTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGGGCAGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAgg                                                                                                                                                                                                                                    <  3:132635/275‑1 (MQ=255)
                                       cATCAACGTGTTAGCAACGTTGAGATTGTTG‑GTGACAATGCGCAAATTGCTGTGATTGAGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgc                                                                                                                                                                                                                                <  2:540571/278‑1 (MQ=255)
                                          cAAGGTGTTATAAAAGTGGAGTTTTTTG‑GTGAAAATGCGCAAATTGGTGTAATTGATCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCAATAAACAGCGTCGAGCCATTTGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgc                                                                                                                                                                                                                                <  3:430796/275‑1 (MQ=255)
                                            cggttgtAGCAATGTGGTGGTTGTTGTGTG‑CAATGCGCAAATTGGTGGGGTTGAGCAGT‑GGGGGCGCTACCTCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGa                                                                                                                                                                                                                           <  4:513101/272‑1 (MQ=255)
                                             cGTGTTAGCAACGTTGAGATTGTTG‑GTGACAATGCGCAAATTGCTGTGAGTGTGCGGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGaa                                                                                                                                                                                                                          <  2:319158/278‑1 (MQ=255)
                                                   aGCAACGTTGAGATTGTTG‑GTGACAATGCGCAAATTGCTGTGATTGAGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCt                                                                                                                                                                                                                    >  2:340424/1‑278 (MQ=255)
                                                         gTTGAGATTGTTG‑GTGACAATGCGCAAATTGCTGTGATTGAGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CTCAGGCTCAGGTTTTGCTCCGCCAGCTCATTgc                                                                                                                                                                                                                                      >  3:270977/1‑253 (MQ=255)
                                                         gTTGAGATTGTTG‑GTGACAATGCGCAAATTGCTGTGATTGAGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGACCAGGTTTTGCTCCGCCAGCCCATTGGGGTCGCGGCGAATGGTCTGCg                                                                                                                                                                                                                 >  3:798242/1‑275 (MQ=255)
                                                                    tg‑GTGACAATGCGCAAATTGCTGGGATTGAGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgag                                                                                                                                                                                                      <  1:624113/275‑1 (MQ=255)
                                                                          aCAATGCGCAAATTGCTGTGATTGAGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCCGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGGGAATAGTCCGCGGGCTGACGGAGAAAt                                                                                                                                                                                                  >  1:87946/1‑274 (MQ=255)
                                                                              tGCGCAAATTGCTGTGATTGAGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTCACCGAACTGGACGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGCCTTACGGAGAGATGctct                                                                                                                                                                                             >  1:28798/1‑275 (MQ=255)
                                                                              tGCGCAAATTGCTGTGATTGAGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTCACCGAACTGGACGGCAGCGCCGCACCGCCACGAT‑TG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCACTGCGGTCGCGGCGAATAGTCCCCGGGCTGACGGAGcaaggact                                                                                                                                                                                              >  3:622553/1‑269 (MQ=255)
                                                                                cgcAAATTGCTGTGATTGGGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg                                                                                                                                                                                        <  2:587998/278‑1 (MQ=255)
                                                                                cgcAAATGGCTGTGATTGAGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg                                                                                                                                                                                        <  2:491488/278‑1 (MQ=255)
                                                                                 actaaTTGCGGTGATTGA‑CGGTGGCGTGCGGTCCCGTTTCGGGGTTGGTGCCGTTATCGGTAAACGTCGTCGAGCCATGGGGTATGTGCTCCGCCACGTTGCGGGCGATGCGCTCTTTTGCTTCGGGCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCATCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg                                                                                                                                                                                        <  4:570152/274‑1 (MQ=255)
                                                                                      ggtgtgtgcatGAGCCGG‑GCGGGCGCTCACGTTGTCGGAGGGGTGGCGATATCGGTAGACAGCGTCGGGCCCTTGGTGATGTGCTCCGCCGCTTTGCGGGCGATGCGGTCTTTTTCTTCGAACAGGGTGGCCTTGCGATCGTGCCACGGCGTGTTGACCGAGCTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                                                                                                                                   <  4:588915/267‑1 (MQ=255)
                                                                                          tgtgATTGAGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGCTCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCCAATAGTCCGCGGGCTGCCGGGGCAATGCTCTCCCGCCTCTTc                                                                                                                                                                                  >  3:285389/1‑274 (MQ=255)
                                                                                            tgATTGAGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                                                                                                                                                             <  2:512172/277‑1 (MQ=255)
                                                                                             ggatgcgtagtgccgtgcgCTACCGTTTCCGGGGTGGTGCCGATAACGATAGAGAGGTACGGGCAATTGGGGATTTGCTCCGGCACTTTGCGGGCGGTGCGCGCGTTTTCGTCGGTCTGGGGGGCCTTGCGATCGTGCCACGGTGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                                                                                                                                                             <  4:508512/264‑1 (MQ=255)
                                                                                                 gAGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa                                                                                                                                                                          <  1:285146/275‑1 (MQ=255)
                                                                                                       t‑gcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAGGCTCTACCCGCCCTTCGGTCCTGCAATaa                                                                                                                                                                     >  3:312401/1‑274 (MQ=255)
                                                                                                       t‑gcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGACCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATATTCTGCGGGCTGACGGAGAAATGCTCTACCAGCCCTTCGGTACTGACCTAACCct                                                                                                                                                                 >  2:260085/1‑278 (MQ=255)
                                                                                                         gtgtgcgGTACCGCGTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGGGGCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                                                                                 <  2:167160/275‑1 (MQ=255)
                                                                                                         gcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGTCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGGTCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGCTTGCGGGCTGACGAAGAAAGGCTCTCCCAGCTCGTCGGTACTGGCATCAACctg                                                                                                                                                                >  4:6654/1‑278 (MQ=255)
                                                                                                         gccggcgcTCCCGGTGCCGGCGTGTTGCTGAGATCGAGAAACTGCGTGGGGCGTGTGTGGATGTTCTGCGCGACATTGTGGGCGATTCGGTCTTTTTCTTCGGTCTGGGTGGCCTTGGGATCGTGCCACGGGGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                                                                                 <  4:545511/273‑1 (MQ=255)
                                                                                                            tgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                                                                                 <  1:312401/274‑1 (MQ=255)
                                                                                                             gcgcTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGCTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCCCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCTAATGGTCTGCGGGCTGACGGAGCACTTCGCTACAAGCTCTTCGGTTCTGACATAACActgc                                                                                                                                                               >  3:509409/1‑275 (MQ=255)
                                                                                                             gcgcTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCTCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CTCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAGTAGTCTGAGGGCTGCCGGAGAAATGCTCTACAAGCTCTCCGTTACTGACATAACCctgc                                                                                                                                                               >  3:806077/1‑275 (MQ=255)
                                                                                                             gcgcTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTCCTGACATAACCctgc                                                                                                                                                               >  1:840155/1‑275 (MQ=255)
                                                                                                             gcgcTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTATTCGTTACTGACATAACCctgc                                                                                                                                                               >  1:740748/1‑275 (MQ=255)
                                                                                                              cgcTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaa                                                                                                                                                                                                    <  3:256249/237‑1 (MQ=255)
                                                                                                              cgcTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGGTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAATTCCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGtt                                                                                                                                                           >  2:324552/1‑278 (MQ=255)
                                                                                                                 gtcccgtttcGGCGTGGTGCCGATATCGCTGAACGGGGGCGCGCCATTGGGGATTTGGTCCGCCGCGTTGCGGGCGATGCGCTCTTTTTCTTCTGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAAAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTa                                                                                                                                                         <  4:203508/268‑1 (MQ=255)
                                                                                                                 gaCCGCTTCCGGCGTGGTGCCGATATCGATAAACGGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTa                                                                                                                                                         <  2:504447/276‑1 (MQ=255)
                                                                                                                  aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTTCTGACTTAACCctgcgtctt                                                                                                                                                          >  1:541947/1‑270 (MQ=255)
                                                                                                                    cGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGTCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAATTGCTCTACAAGCTCTTCGGTACTGACATAAcccctacggttca                                                                                                                                                        >  3:149042/1‑265 (MQ=255)
                                                                                                                      cTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                                                                                                                                   <  1:714073/246‑1 (MQ=255)
                                                                                                                       ttCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc                                                                                                                                                       <  1:535080/273‑1 (MQ=255)
                                                                                                                                 gtgCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGCT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCa                                                                                                                                                                                         >  2:2315/1‑229 (MQ=255)
                                                                                                                                 gtgCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGACATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataat                                                                                                                                           >  1:628617/1‑275 (MQ=255)
                                                                                                                                  tgacGAGATCGATACACAGCGTCGGGCCATTGGGGATCTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTATTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataata                                                                                                                                          <  1:285389/272‑1 (MQ=255)
                                                                                                                                     cGATATCGAGAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCg                                                                                                                                       <  1:149042/275‑1 (MQ=255)
                                                                                                                                       atatCGATAAACAGCGTCGTGCCATTGGGGATTTGCTCCGCCCCTTTGCGTGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt                                                                                                                                     <  1:569943/275‑1 (MQ=255)
                                                                                                                                            gataacaggCGTCGAGCCATTGGGGATTTGCCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGt                                                                                                                              <  2:368824/269‑1 (MQ=255)
                                                                                                                                                 aCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAACTGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgt                                                                                                                           >  3:515266/1‑275 (MQ=255)
                                                                                                                                                   aGCGTCGGGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTgtgt                                                                                                                         <  1:751728/275‑1 (MQ=255)
                                                                                                                                                     ggctatgcccgtGGGGGTTTGGCCCTCCCCTCTGGGGGAGATGCGCTTGTTTTTGTCGGTCTGGGTGGCGTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgtt                                                                                                                    <  4:518149/267‑1 (MQ=255)
                                                                                                                                                      gTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgttt                                                                                                                       >  1:126247/1‑274 (MQ=255)
                                                                                                                                                      gTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgttt                                                                                                                       >  1:203956/1‑274 (MQ=255)
                                                                                                                                                      gTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtt                                                                                                                        >  3:296697/1‑273 (MQ=255)
                                                                                                                                                        cGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgtt                                                                                                                    <  1:296697/275‑1 (MQ=255)
                                                                                                                                                          aGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTCTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGCAGGAAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCTAGCCCATTGAGGTCGGGGCGAATAGTCTGCGGGCTGACGGAGAAATGCCCTACAAGCCCTTCGCTCCGGACATACCCCTGCTGTTGAACACGTCCGATAATATCTCTGTGACGTCGTGTTTTTTTc                                                                                                                  >  4:200111/1‑275 (MQ=255)
                                                                                                                                                               ttGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATaa                                                                                                                                                                     >  1:399578/1‑219 (MQ=255)
                                                                                                                                                               ttGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATCCTCTACCAGCTCTTCGGTACTGACATCACCCTGCTGTTTAACCAGTTCGATAATACCTTTGTGCCGTTGTGTTTGTTTCttttt                                                                                                             >  3:433296/1‑274 (MQ=255)
                                                                                                                                                               ttGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAGGCTCTAGCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGGTTCGTTTATaa                                                                                                          >  2:406999/1‑278 (MQ=255)
                                                                                                                                                                   ggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                                                                                 <  3:399578/219‑1 (MQ=255)
                                                                                                                                                                     aTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATccc                                                                                                     <  2:513641/277‑1 (MQ=255)
                                                                                                                                                                               ccACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCCGCTCATTTAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGATCTACCAGCTCTTCGGTACTGACATAACCCAGCTGTTTAAGCAGTTGGCAAATAAAGTTGTAACTGTGTGTTTGTTTCTTTTTTCAATCCCTGGAattttt                                                                                           >  4:249119/1‑277 (MQ=255)
                                                                                                                                                                                     tGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATTATACCGTTGTTACGTTTTGTTTGTTTCATTTATAAATCCCTGGAATTCtttttg                                                                                       >  3:775547/1‑273 (MQ=255)
                                                                                                                                                                                     tGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTGGAATTAttttcg                                                                                       >  1:380016/1‑275 (MQ=255)
                                                                                                                                                                                            gATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCAATGAT‑GG‑CGCAGGATCAGGTTTTACTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGACATGCTCTCCGAGCTCTTCGGTACTCACATAACCCGGCCGCTTAACCAGTTCGCGAATACCCCTGTGATGTTGTGTTTGTTTCATTTCTAAATCCCTGGCATTtttttctttttctcac                                                                              >  4:373083/1‑273 (MQ=255)
                                                                                                                                                                                               gcgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGAGCCGCACGGCCATGATGGG‑G‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCa                                                                             <  3:293802/275‑1 (MQ=255)
                                                                                                                                                                                                     tttttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGa                                                                     <  2:23726/277‑1 (MQ=255)
                                                                                                                                                                                                       gtgcttcGGGCTGGGTGGCACTTCGATCGTGCCACGGGGTGTTAACAGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt                                                                   <  2:56251/274‑1 (MQ=255)
                                                                                                                                                                                                               gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaaa                                                           >  2:85027/1‑277 (MQ=255)
                                                                                                                                                                                                                 cTGGGGGGCCTGGCGGTCGTGGCACGGCGTGTTAACCGAACTGGAAGGAAGCGCCGCACCGCCATGAT‑GGAG‑CAGGATCAGGTTTTGATCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc                                                        <  4:440025/278‑1 (MQ=255)
                                                                                                                                                                                                                   gggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGt                                                                                                                              <  3:356158/206‑1 (MQ=255)
                                                                                                                                                                                                                    ggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc                                                        <  3:126247/275‑1 (MQ=255)
                                                                                                                                                                                                                       ggCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGTATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                                                  <  2:24791/278‑1 (MQ=255)
                                                                                                                                                                                                                          cTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAACGCCATCGCTaa                                                >  4:536511/1‑277 (MQ=255)
                                                                                                                                                                                                                               ggtCGGGCCACGGGGGTTAAACCGAACTGCAAGGCAGCGCCCGACCGCCATGAT‑GG‑CGAAGGGTCAGGTTTTGCTCCGCCAGCTCATTGGGGTCGCGGCGAATAGTCGGCGGGCTGACGGAGAAATGCTCTACCAGCTATTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAcc                                             <  3:823356/273‑1 (MQ=255)
                                                                                                                                                                                                                                   gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACg                                         >  1:66688/1‑275 (MQ=255)
                                                                                                                                                                                                                                      ccACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAc                                   <  2:536511/278‑1 (MQ=255)
                                                                                                                                                                                                                                      caccggcggGTTAACCTAACTTGAAGGCAGTGCCGCACTGCCATGGT‑GG‑CGCATGGTCAGGTTTTGCTCCGCCAGCTCATTGAGGTGGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAACAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAc                                   <  4:356762/275‑1 (MQ=255)
                                                                                                                                                                                                                                        aCGGCGTGTTAAACGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGAGCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTa                                     <  1:251996/274‑1 (MQ=255)
                                                                                                                                                                                                                                         cggcggGTGAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGGCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAc                                   <  1:123293/275‑1 (MQ=255)
                                                                                                                                                                                                                                         cGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGTAATGCTCTACCAGCTCTTCGGTACTGACATAACCTTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCTCCAAAAGCCATCTCTAAACCCTCGGCTa                                     >  1:91555/1‑273 (MQ=255)
                                                                                                                                                                                                                                            cGTGTTTACACATCTGGGAGGCAGCGGCGCACCGCCATGAT‑GG‑CGGAGGATCAGGTTTTGGTCCGCCAGCTCATTGAGGTCGGTGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTNTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGc                              <  4:340424/277‑1 (MQ=255)
                                                                                                                                                                                                                                                        aCTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTTTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGTATCAACAAAAGCCATTCCTAAACCCACGGTTACCCCgccaattgtgcca                    >  3:44510/1‑263 (MQ=255)
                                                                                                                                                                                                                                                             aaGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATATCCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGt                <  1:62999/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                  aGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCg       >  2:265135/1‑278 (MQ=255)
                                                                                                                                                                                                                                                                    cgccgcACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATc        >  1:581393/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                    cgccgcACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCCCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTTTGTTCCGTTCTCCAtg        >  1:746989/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                        gcACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATc  <  2:610421/277‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 tgat‑gG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAccc                                                                                                                                                                  >  2:155340/1‑108 (MQ=255)
                                                                                                                                                                                                                                                                                  gat‑gG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                                                                                 <  4:155340/108‑1 (MQ=255)
                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                    
TTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTG‑GTGACAATGCGCAAATTGCTGTGATTGAGCAGT‑GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATC  >  NC_000913/3560178‑3560715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑