Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,3801.C96.9% 301.1 / 2.6 97intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (3/0);  new base C (46/47);  total (49/48)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
*NC_0009134,296,3802.G96.9% 292.9 / 2.7 97intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (3/0);  new base G (46/47);  total (49/48)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

TATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TA‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGT  >  NC_000913/4296153‑4296644
                                                                                                                                                                                                                                     ||                                                                                                                                                                                                                                                                           
tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATATCCTGATTTATCGTGATTTTGTAGGTCGGATAAGTCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCCGGCTTACGCCTGACAGCCAAAGAGCCTGATTTAGCTTGATTTTGTAGGTCTGATAAGGCGTT‑TACGC‑C‑GAATCTGACCTCACCCCCTGATTTGACGCCTGCCCAGTCTTactcg                                                                                                                                                                                                                           >  4:283154/1‑274 (MQ=11)
                         gATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTa                                                                                                                                                                                                      >  1:652490/1‑274 (MQ=9)
                          ttGCGACGCTTCCCACGTCTTATCCAGCCTACGCCAGACAGCGCAATTGGCTGATTTAGCGCGATCTTGTCGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGACGCGTCGCCTGACGCGTCTTATCCGGACTACGCCATACAGCGCAATAGCCTGATTTACCGTCATTTTTTACGTCGTATCAGTCGTT‑TACGC‑C‑GCATCCGCCATCAATGCCTTATGCTACCCTTCCCTCGTCTTCTCAGCCCTATATTAAACGTTGGTTAAt                                                                                                                                                                                                    >  3:737038/2‑275 (MQ=2)
                          aTGCGACGCTTGCCGCTTCTTATCAGGCCTACGCCAGACAGCGCAATTGCCTGTTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAt                                                                                                                                                                                                    <  1:690791/275‑1 (MQ=255)
                                  cgtgccgcgTCTTATCAGGTCTACGCCAGACAGCGAAGTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCAGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                                                                                                                                                            <  1:713963/270‑1 (MQ=255)
                                     gccgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGCCGCTTGCCGCGGCTTATCAGGCCTCTCCTAACCGTTGGTTAATTTTTCAAAct                                                                                                                                                                                          >  1:255247/3‑274 (MQ=255)
                                           tCTTGTCATGCCTACGCCAGACTGCACAATGACCTGATTTAGAGTGATTTTGTAGGTCGGATAAGGCGTTTCTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCGGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑TCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                                                                 <  2:96803/277‑1 (MQ=14)
                                           tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCAGCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                                                                 <  2:133861/277‑1 (MQ=255)
                                           tCTTAGCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTGGAGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGACTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                                                                 <  2:283154/277‑1 (MQ=255)
                                              tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAccc                                                                                                                                                                                <  1:62161/275‑1 (MQ=255)
                                                  aGGCCGACGACAGAACGCGCAATAGCCTTATTTTGCGTGATTTGGTAGGTGGAATAAGGCGTTTATGCCGCATCCGACACAAACGCCTGAGGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTa                                                                                                                                                                           <  1:92681/275‑1 (MQ=14)
                                                    tccTACGCCAGACAGCCCAAGAGCATTATTTAGCTTGGTTTTGGAGGCCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAACCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCaaa                                                                                                                                                                      <  2:613005/277‑1 (MQ=14)
                                                     ctgatgccagcaggcgaAATAGCTTGATTTGCGGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGACTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCaaa                                                                                                                                                                      <  4:618748/264‑1 (MQ=14)
                                                     ccTAGGGCAGACAGCGCAATAGCCGGATTTGACGTGATTTTGTTGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTGTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGAAGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCa                                                                                                                                                                        <  3:335279/275‑1 (MQ=14)
                                                        ccGCCAGAAAGCGCAATAGCCTGATTTAGCGTGATTTTGTCGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCaaa                                                                                                                                                                      <  1:127490/273‑1 (MQ=255)
                                                        aacccAGAGAGCGAAGTACCCTGCTTTAGCTTGATTTTGCAGGTGGGATAAGGTGTTAAGCACGATGCCGAGTTAAAGGCCTGATGAGACGTTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCaaa                                                                                                                                                                      <  3:666098/271‑1 (MQ=14)
                                                        aCGGCAGACAGCGAAATAGCCTGATGTAGCGTGATTTTGAAGGTCGGATAAGGCGTTTAGGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCTCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCaaa                                                                                                                                                                      <  1:339115/274‑1 (MQ=14)
                                                        aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCaaa                                                                                                                                                                      <  1:245610/274‑1 (MQ=255)
                                                                   cgcATTAGCCTGATTTAGGTTGATTTTGTGGGTCGGATAAGGCGTTGATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATa                                                                                                                                                            <  1:283878/273‑1 (MQ=255)
                                                                   cgcAATAGCTTGATTTACCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTAATGCGACGCTTAACTCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATa                                                                                                                                                            <  1:513123/273‑1 (MQ=255)
                                                                   cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCTACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATCGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGAACCTTGCCGCGTCTTATCAGGCCTATCTTAACCTTTGGTTAATTTTTCACTCTCTTCACACCC‑GGTCTCAAACCTTTccctcaagc                                                                                                                                                        >  2:487120/1‑270 (MQ=14)
                                                                   cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCTGACATCAATGCCTGATGCGCAGCTTGCCGCGTCTTATCAGGCCTATATTAACCGCTGGTTAATTTGTCAACCTCTTCACACTC‑GGTCTCAACCCCTTcactacctct                                                                                                                                                       >  4:366425/1‑269 (MQ=14)
                                                                   cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACa                                                                                                                                                          >  3:398353/1‑275 (MQ=255)
                                                                   cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAAACCC‑GGTATCAAACCCTTCCATACa                                                                                                                                                          >  1:271972/1‑275 (MQ=255)
                                                                   cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTC‑TACGC‑C‑GCGTCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCATAACCGTCGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCt                                                                                                                                                       >  2:578091/1‑278 (MQ=255)
                                                                   cgcAATAGCCTGATTTAGCGTGATTTTGTACGTCGGTTAAGCCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCTTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTTAATCTGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TA‑‑CGCTGCTTCCTACTTTCATGGCTGTTGCGCCGCTTGACGCCTCTTACCAAGCCTATCTTCACCGTTGGTTAATTTTTAAAACTCTTCACACCC‑GGTATCAAACACTTCCATaaa                                                                                                                                                          >  3:395099/1‑273 (MQ=14)
                                                                    gagatagtgtggttttGCGTGTATTTGAGGGTCTGACAATGCGTTTAGGTCGCATGCGACATCTACGCCGGATGCGACGCTTGACGCGTCTGATCAGGCCTACGCCAGAGAGCGAAATAGCCTGTTTTAGCGTTCTTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCt                                                                                                                                                       <  4:559064/265‑1 (MQ=17)
                                                                      aaTAGCCTGATTTAGCGTGATTTTGTTGGTCGGATAAGACGTTTGTGCCGCATCCGACATCAACGCCGGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCt                                                                                                                                                       <  1:404170/275‑1 (MQ=255)
                                                                      aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCt                                                                                                                                                       <  3:265543/275‑1 (MQ=255)
                                                                        tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGCTAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTACTTTTTCAAACTCTTCACACCC‑GGTATCAAATCCTTCCATACAGccc                                                                                                                                                      >  3:826057/1‑272 (MQ=255)
                                                                             tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTca                                                                                                                                                                                     >  3:619571/1‑239 (MQ=255)
                                                                             tGATTTAGCGGTATTTTGTAGGGTGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAAGCGGCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAGTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTACCCGTTGGTTAATTTTTCAAACTCTGCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAAc                                                                                                                                             <  2:455349/278‑1 (MQ=17)
                                                                              gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAGCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCaaa                                                                                                                                                                      <  2:125695/252‑1 (MQ=255)
                                                                              gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAACCTCTTCCCCCCG‑GGTAACAAACCCTTCCATACAGCTCATGTTc                                                                                                                                                >  3:515937/1‑274 (MQ=17)
                                                                                 ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                                                                 <  1:619573/239‑1 (MQ=255)
                                                                                 ttAGCGTGATTTGGTAGGTCGTGTAAGGCGTTTTACCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCGGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAAc                                                                                                                                             <  1:433725/274‑1 (MQ=18)
                                                                                     cGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATCAAGCTCAGGTTCAACCAGTg                                                                                                                                        >  1:199437/1‑275 (MQ=255)
                                                                                     cGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAg                                                                                                                                     >  2:160638/1‑278 (MQ=255)
                                                                                         aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCATTGCAGCAcc                                                                                                                                 >  2:193985/1‑278 (MQ=255)
                                                                                         aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAgc                                                                                                                                    >  3:819768/1‑275 (MQ=255)
                                                                                         aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAg                                                                                                                                     <  1:398353/274‑1 (MQ=255)
                                                                                          ttttGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAGGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAg                                                                                                                                     <  1:549132/273‑1 (MQ=255)
                                                                                          tgtggtgagTCGGATAAGGCGTTTATGCCGTGTCCGACATCAACGCCTGGTGCGCCCGTTACCGCGTCTTATCAGGCGTATGCCAGACAGCGCAATGGCCGGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACCC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAg                                                                                                                                     <  1:412387/265‑1 (MQ=18)
                                                                                                 ggTCGGATAGGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGc                                                                                                                           <  2:212486/276‑1 (MQ=255)
                                                                                                            gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCACACTCTTCACACCC‑GGTCTCAAACCCTTCCATACACCTCCAGATTAACCAGTGCAGCGCCTTTTGCTTTTTCTCCTCgt                                                                                                              >  4:203283/1‑277 (MQ=18)
                                                                                                               tttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATGTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTATTACCAGGCCTATCTTAACCGTCGGTTACTTTTTCAACCTCTT‑ACACCCAGGTACCAACCCCTTCCAAACATCTCAGGTTCAACCAGTCCGACGCCTTCTGCTTGTTCTTATGGATa                                                                                                            >  4:137374/1‑277 (MQ=17)
                                                                                                                ttATGCCGCCTCCGACATCAACTCCTGATGCGACGCTTAACGCGTCTTATTAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTATGTCGGATAAGGCGTT‑TA‑‑CGCGGCATCCGACATCAATTCCTGATGCGACCATTGCCGCGCACTATCCGGCCTATCTTAACCGTTGGTTAAttttt                                                                                                                                                                                                >  1:813916/1‑193 (MQ=14)
                                                                                                                ttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGa                                                                                                              >  1:51487/1‑274 (MQ=255)
                                                                                                                 tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCCTAAAGTTCAGTTTACACCACTGCCACGCCTTTTGCTTGTTCTTTTCGa                                                                                                              >  3:676246/1‑273 (MQ=18)
                                                                                                                    gCCGCATCCGACATCACCGCCTGAGCGGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATaaa                                                                                                          <  3:571549/274‑1 (MQ=255)
                                                                                                                      cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCCAACCCTTCCATACATCCCCGGTTCCACCCGTCCCGCCCCTTTTGCTTCTTCTTCTCGATACAAActtc                                                                                                    >  4:124837/1‑278 (MQ=18)
                                                                                                                        cctCCGACATCAACGCGTGCGTGTACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACcttctt                                                                                                  <  2:454626/276‑1 (MQ=21)
                                                                                                                             gACACCAACGCCTGATGCGACGCTTAACGCGTGTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTGTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATCCAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCa                                                                                                <  1:843912/275‑1 (MQ=255)
                                                                                                                                   aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGATGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGATCAGGt                                                                                                                                                  >  3:543025/1‑219 (MQ=255)
                                                                                                                                   aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGt                                                                                                                                                  <  1:543027/219‑1 (MQ=255)
                                                                                                                                           aTGCGACCTCTAAAGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAcat                                                                                  <  1:78916/275‑1 (MQ=255)
                                                                                                                                            tGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGTTAAAACCTTCTTCACCGTTCAAGAAcct                                                                                  >  3:598203/1‑272 (MQ=255)
                                                                                                                                             tcGACCCTTAACCCGGCTTTTCCGGCCTACGCCAGACAGCGCAAGTGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCTTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTc                                                                             <  2:137374/277‑1 (MQ=25)
                                                                                                                                                aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAACCTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCCCAGGTTCAACCAGTTCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTc                                                                             >  4:394754/1‑275 (MQ=255)
                                                                                                                                                   tgcaaCGCGTCGTATCAGGCAGAAGCCAGAAAGGGAGAGATGCTGATTTTGAGTGAATTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGACTGATGCGAGGCTTGCCGCGTCTTATCAGGTCTATCTGAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAGCCTTCTTCACCGTTTAAGAACATCATTCCCGccc                                                                       <  4:513354/275‑1 (MQ=25)
                                                                                                                                                     tAACGCATCGTATCAGGCCTCCGCCAGACAGTGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCTACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGcc                                                                        <  1:304495/275‑1 (MQ=255)
                                                                                                                                                      aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGcc                                                                        >  1:328553/1‑274 (MQ=255)
                                                                                                                                                                 tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATaa                                                         <  2:433645/278‑1 (MQ=255)
                                                                                                                                                                  cAGGCCTACGCCAGACAGCTCTATAGCCTGAGTTAGCTTGCTTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAAc                                                        <  2:52605/278‑1 (MQ=255)
                                                                                                                                                                     gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGAGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCCCATCC‑GGTATCACACCCTTCCATACAGCTCAGGTTCAACCAGTGCCGCGCCTTTTGCTTGTTCTTCTCGATAAACCCTTCTTCACCGTTTATGAACATCATTCCCGCCCATTACTCGGATTaa                                                         >  3:786922/1‑274 (MQ=25)
                                                                                                                                                                      ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAAc                                                        >  3:399250/1‑274 (MQ=255)
                                                                                                                                                                       cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTACG‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTCTTCTTCTCGATAATACCTTCTTCACCTTTTAAGAACAACTTTCCCCCCCATTCCTAGGAATAAcagtgcg                                                  >  4:148781/1‑272 (MQ=25)
                                                                                                                                                                        caggCCATACAGTGTAATTGCCTGATTTTGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGtt                                                   <  4:466815/274‑1 (MQ=38)
                                                                                                                                                                         aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCg                                                 <  2:521158/278‑1 (MQ=255)
                                                                                                                                                                          cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTTCAGCGCCTTTTGCTTGTGCTTCTCGATAAAACCTTCTTCACCGTTTAGTAACATCTTTCCCGCCCAGTCCCCGGATATACCGGt                                                    >  1:342558/1‑274 (MQ=25)
                                                                                                                                                                          cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTACCCTACAGCTCAGGTTCACCCCGTCCCGCGCCTTTTACTTGTTCTTCTCGATAAAACCCTCTTCACCGTTTACGACCATCCTTCCCCCCCCGTACGCGGACTCACCCGtcacg                                                >  4:208214/1‑274 (MQ=21)
                                                                                                                                                                               gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGa                                              <  1:399250/275‑1 (MQ=255)
                                                                                                                                                                                 cAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACACCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGaaa                                            <  2:352745/275‑1 (MQ=255)
                                                                                                                                                                                    cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCTAACTCTTCACACCC‑GGTCTCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCCACAAAACCCTATTAACCGTTTAATAACCTCATTCCCGCACCGTACTCGGAATAACTGGCTCGGGAAAtgtcga                                      >  4:403877/1‑273 (MQ=25)
                                                                                                                                                                                    cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGtt                                                                                           >  4:394146/1‑225 (MQ=255)
                                                                                                                                                                                    cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAATATCATTCCCGCCCAGTACTCGTAATAACCGGTTCGGGAACTTGCat                                      >  2:248509/1‑277 (MQ=255)
                                                                                                                                                                                    cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTg                                         >  1:627356/1‑275 (MQ=255)
                                                                                                                                                                                    cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACAGCTTGGGGAAAGTGCag                                      >  4:456062/1‑278 (MQ=255)
                                                                                                                                                                                     gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGttt                                                                                          <  2:394146/225‑1 (MQ=255)
                                                                                                                                                                                        aTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGTCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCt                                  <  2:124837/278‑1 (MQ=255)
                                                                                                                                                                                        aTAGCCTGATTGAGCGTGGATTTGTAAGTCGGATAAGGCGTT‑TAAGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCACGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAACC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCt                                  <  4:440456/278‑1 (MQ=255)
                                                                                                                                                                                              tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGCACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTTCacaacttcgctt                            >  2:53086/1‑267 (MQ=255)
                                                                                                                                                                                              tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTTCg                               >  1:200812/1‑275 (MQ=255)
                                                                                                                                                                                              tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCg                               >  1:227406/1‑275 (MQ=255)
                                                                                                                                                                                                aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGtt                             >  1:805692/1‑275 (MQ=255)
                                                                                                                                                                                                aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTCCGGGAAATTGCAGAACTGCGtt                             >  1:7591/1‑275 (MQ=255)
                                                                                                                                                                                                aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCACGTTCAACCAGTGCACCGCCTTTTCCTTGTTCTTCTCGATAAAACCTTCTTAACCGTTTAAGACCATCATTCACGCCCACTACTCGCAATAACCGGTTCCGGAAATTCCAAAGcctccttg                            >  4:573786/1‑269 (MQ=37)
                                                                                                                                                                                                      cgggATTTTGTAGGTCGGATAACGCGTT‑TACGC‑C‑GCATCCGAAATCAATGCCTGAGGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAAt                       <  3:62426/272‑1 (MQ=255)
                                                                                                                                                                                                         gagtttGTAGGTCGGAGAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATAcc                    <  3:330716/272‑1 (MQ=255)
                                                                                                                                                                                                          aTTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCTGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTATTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCTCCTTTTGCTTGTTCTTCTCGCTAAAACCTTCTTCACCGTTTACGAACTGCATTCCCGCCCAGTACTCGTAACACCCGGTCGGGGAAATTGCATAGCTCCGTTTGAACTAcc                    >  3:323294/1‑274 (MQ=37)
                                                                                                                                                                                                          aTTTTGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTTCTTGTTCTTCTCGCTAAAACCTTCTTAACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATGGCATAGCTTCATTTGTAATAcctgt                 >  2:415032/1‑274 (MQ=38)
                                                                                                                                                                                                            tttGTAGGTCGGATAAGGCGTT‑TACGC‑C‑GCATCCGACCTCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTTCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAgg                 <  3:152390/275‑1 (MQ=255)
                                                                                                                                                                                                                     cGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCACACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACAGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAAGCGGTCCGGGAAATTCCAGAGCTGAGCTTGAAATACCAGGTTGCCTTGTCAt     >  2:7938/1‑278 (MQ=39)
                                                                                                                                                                                                                     cGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGCTAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTTGGGAAATTGCAGAGTTGCGTTTGAAATCCCAGGTTGCCTTGt        >  3:92146/1‑275 (MQ=255)
                                                                                                                                                                                                                     cGGATAAGGCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACCCTTCCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTCTCAAACCCATCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTACTTGTTCGTCTCGATACAACCTTCTTCACCGTTCAAGAACATCATTCACGCCCAGTACTCGTAATAACCGGTTCGGGGAAGTGCATAGCTGCGTTTGAAATACACGGTCGCCTTGt        >  3:829030/1‑275 (MQ=38)
                                                                                                                                                                                                                       gATAAGGCGTT‑TACGC‑C‑GCATCCGAAATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTc       <  1:540281/274‑1 (MQ=255)
                                                                                                                                                                                                                            ggCGTT‑TACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCCGTACTCGGAAGAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCAtcgt  >  1:346175/1‑274 (MQ=255)
                                                                                                                                                                                                                                     ||                                                                                                                                                                                                                                                                           
TATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑TA‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCC‑GGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGT  >  NC_000913/4296153‑4296644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑