BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	257,908	Δ776 bp		[crl]	[crl]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	257908–258674	258683	10–776	137 [0]	[0] 136	[crl]	[crl]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 257907	0 (0.000)	127 (0.860)	79/506	0.4	100%	intergenic (+8/‑769)	crl/crl	pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
*	?	NC_000913	258684 =	0 (0.000)	127 (0.860)	79/506	0.4	100%	pseudogene (9/331 nt)	crl	pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

Only 100 of 136 total aligned reads displayed.

CATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTG  >  NC_000913/258438‑258958

catcatctgctgacggtaaattattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatagaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggaccaccgaagagcagattgatcaaaaaatttaccgcattagcgccgtttattcgttaaggtaAG‑TGCAACGCAAATCGATTCTTTTTCGAt                                                                                                                                                                                                                                                          >  3:522021‑M2/247‑275 (MQ=255)

  tcatctgctgacggtaaattattagagatcccatttatcccggtgtatcggcattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgggaaggtaAGTTACAAAG‑TAATCGATTCTTTTTCGATTg                                                                                                                                                                                                                                                        >  1:234677‑M2/245‑275 (MQ=255)

  tcatctgctgacggtaaattattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTg                                                                                                                                                                                                                                                        >  1:460831‑M2/245‑275 (MQ=255)

    atctgctgacggtaaattattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGt                                                                                                                                                                                                                                                       <  3:36389‑M2/32‑1 (MQ=255)

    atctgctgacggtaaattattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                                                                                                                                                                                                   <  2:66583‑M2/36‑1 (MQ=255)

       tgctgacggtaaattattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaatttttccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                                                                                                                                                                                                   <  1:853041‑M2/36‑1 (MQ=255)

        gctgacggtaaattattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑GGCAAAGATAATCGATTCTTTTTCGATTGTCTGGc                                                                                                                                                                                                                                                  >  1:578847‑M2/239‑275 (MQ=255)

                    attattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgacaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcagcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaacgcactaggaccgtatattcgtgaagttaAG‑TGCAAAGATAATTGATTCATTTTCgattttctggcttactgggtcat                                                                                                                                                                                                                                      >  1:794061‑M2/227‑264 (MQ=255)

                       ttagagatcccatttaacccgggtgatcggaattttggcaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttttgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGt                                                                                                                                                                                                                                   <  3:227924‑M2/52‑1 (MQ=255)

                        taatgtgcccatttaacccggtgtatcggaattttgacaaaggacgtcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaatgttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGt                                                                                                                                                                                                                                   <  3:658783‑M2/52‑1 (MQ=255)

                              tcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagttgacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAAAGTGaaa                                                                                                                                                                                                                               >  1:233950‑M2/217‑272 (MQ=255)

                              tcccatgtaacccggtgtatcggaattttgaaaaagttcttgatgaaatcaaccactggatcgaaaaacgcttgtgttaaaaatttgctgaagtttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggaaacccgaagagcagattgataagaaaatttaccgcactgggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAcc                                                                                                                                                                                                                             <  3:855325‑M2/58‑1 (MQ=255)

                               cccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactgtatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttacctagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggccagtatattagtgaaggtaAG‑TGCAAAGATAATCGATTTTTGTTCTATTGTCTTGATGTAGGCGTCAacgtgaatctgg                                                                                                                                                                                                                           >  1:612690‑M2/216‑270 (MQ=255)

                               cccatttaacccggtgtatcgcaattttgacaacggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaattttctaaattttgccaatttggtgaaacagttgcatcacacccggagatagcaatgacattaccgagtggacacacgaagagcagattgatcaaaaactttaccgcactaggaccgtatatttgtgaagttaAG‑TGCAAAGCTAATGTGTACATTTTCGCGTGTCCGGTTGTATGATTCAACTTGAAaccggtgc                                                                                                                                                                                                                        >  4:444928‑M2/216‑275 (MQ=255)

                                     taacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCgg                                                                                                                                                                                                                     <  1:574181‑M2/66‑1 (MQ=255)

                                            gtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTg                                                                                                                                                                                                                                                        >  2:117704‑M2/203‑233 (MQ=255)

                                            gtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGt                                                                                                                                                                                                                                         >  1:661081‑M2/203‑248 (MQ=255)

                                            gtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCg                                                                                                                                                                                                              >  3:577779‑M2/203‑275 (MQ=255)

                                            gtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACAGGCACCGGAAGTGCg                                                                                                                                                                                                              >  2:198929‑M2/203‑275 (MQ=255)

                                            gtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTAAACGTGAAACCGGCACCGGAAGTGCg                                                                                                                                                                                                              >  2:424016‑M2/203‑275 (MQ=255)

                                            gcgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaaccggagatagcaatgacgttaccgagtggacacccgcagagcagatggatcaaaaaatttaccgcactaggcccgtatatacgtgcaggtaAG‑TGCAAAGATAAGCGAATCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCAGCGGGAGTGCg                                                                                                                                                                                                              >  4:539168‑M2/203‑275 (MQ=255)

                                             tgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgacggtaAG‑TGCACAGATAATCGATTCTTTTTAGATTTTCTGGCTTTATGCTTAAATGTGAAACCGGCACCGGAGGTGCGt                                                                                                                                                                                                             >  3:18619‑M2/202‑275 (MQ=255)

                                             tgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcataacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTATGGCTGTATGCGTCAACGTGAAACCGGCACCGGCAGTGCGTGaa                                                                                                                                                                                                          >  2:415870‑M2/202‑278 (MQ=255)

                                             ggtatcggaaatttgacaaaggtcttcaggaaataaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGt                                                                                                                                                                                                                                                       <  4:117704‑M2/32‑1 (MQ=255)

                                                       ttttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATCATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTTAATTCTgg                                                                                                                                                                                                    >  1:124117‑M2/192‑274 (MQ=255)

                                                                      taaggaaataacaaactggctacaaaaacgcatgagttaaaaattttctaaattttgccaatttggtaaaacaggtgcatcacaaaaggagatagcaatgacgttagcgaaaggaaacacgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTgg                                                                                                                                                                                 <  4:435532‑M2/102‑1 (MQ=255)

                                                                           aaatcaccgactggatcgaaaaacgctttggttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATAGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGaa                                                                                                                                                                               <  3:341895‑M2/104‑1 (MQ=255)

                                                                            aatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGgaat                                                                                                                                                                              >  1:415263‑M2/171‑274 (MQ=255)

                                                                             atcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                                                                                                                                                          >  2:96985‑M2/170‑278 (MQ=255)

                                                                             atcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGGGGATGGAGCTGGAAGCGCa                                                                                                                                                                          >  4:186672‑M2/170‑278 (MQ=255)

                                                                                      tagttagaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaattacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATccc                                                                                                                                                                  <  2:411672‑M2/117‑1 (MQ=255)

                                                                                        gatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgaatattcgcgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGGTGGAAGCGCAGgaatccgggt                                                                                                                                                               >  4:291803‑M2/159‑274 (MQ=255)

                                                                                        gatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagagtgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGTTGGAGCTGGAAGCGCAGGAATccc                                                                                                                                                                  >  1:789440‑M2/159‑275 (MQ=255)

                                                                                        gatagaaaaacgattgtgttaaaaatttgataaattttgccaatttggtaaaacaggtgcataaaaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatgtaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGtt                                                                                                                                                               <  4:108202‑M2/120‑1 (MQ=255)

                                                                                          tcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGt                                                                                                                                                                <  1:493160‑M2/119‑1 (MQ=255)

                                                                                                   gcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAcctcc                                                                                                                                                       >  1:181079‑M2/148‑273 (MQ=255)

                                                                                                        tgttaaaaatttgctaaattttgccaatttggtaaaacagttacatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTa                                                                                                                                                  >  3:514391‑M2/143‑275 (MQ=255)

                                                                                                                atttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTg                                                                                                                                          <  3:625532‑M2/141‑1 (MQ=255)

                                                                                                                            ttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATa                                                                                                                              <  1:166944‑M2/153‑1 (MQ=255)

                                                                                                                                  atttggtaaaacagttgcatcacaacagaagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaagttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAg                                                                                                                        <  1:123787‑M2/159‑1 (MQ=255)

                                                                                                                                     tggtaaaacaaatggatcaaaaaaagagctagcaaacaccttaccgagtggacacccgaagagcagattgatcaacaaagttaccgcactaggcccgtttattcgtgaaggtaCG‑TGCAAAGATAATCGATTCTTTTTCGATTTTCTGGTTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGc                                                                                                                      <  3:794059‑M2/161‑1 (MQ=255)

                                                                                                                                              agttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAag                                                                                                             <  1:577781‑M2/170‑1 (MQ=255)

                                                                                                                                                ttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGAAACGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACTTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGacgggaaggaggtt                                                                                                       >  4:593000‑M2/103‑272 (MQ=255)

                                                                                                                                                 tgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAgtg                                                                                                         >  1:136074‑M2/102‑275 (MQ=255)

                                                                                                                                                  gcaacgcaacaggagatagcaatgacgttaacgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCTTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGTTAAAGCAGGCGACTGGAAGAgtgt                                                                                                        <  3:415263‑M2/175‑1 (MQ=255)

                                                                                                                                                    atcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGaa                                                                                                              <  3:746373‑M2/169‑1 (MQ=255)

                                                                                                                                                         tacatgaaatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcacttggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGTTTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTa                                                                                                <  1:574840‑M2/182‑1 (MQ=255)

                                                                                                                                                          acaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAg                                                                                            >  4:621602‑M2/93‑278 (MQ=255)

                                                                                                                                                                   agcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTTCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGAGGGAAGCCCGTGTTACCTACAGTTACCAGTTGGGTCTGTTGGATAACGCAGGCGGATGGACGAGTGTTC‑CGGTAAAAGACAGtg‑agg                                                                                  >  4:247589‑M2/84‑276 (MQ=255)

                                                                                                                                                                   agcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGATTTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑aa                                                                                   >  2:379094‑M2/84‑277 (MQ=255)

                                                                                                                                                                   agcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑aa                                                                                   >  2:227483‑M2/84‑277 (MQ=255)

                                                                                                                                                                   agcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGAGTGATTTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTGGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTGT‑CGGTAAAAGACActc                                                                                      >  1:386130‑M2/84‑274 (MQ=255)

                                                                                                                                                                        tgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCACAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACAGGAAGTGCGTGAATTCTGGGGCTGGTGGCTGGAGCTGGAAGCGCAGGAATCCAGTGTTACCTACAGTTCACAGTTTGGTCTGTTCGATAAAGCAGGCGCATGGAAGA‑CGTTCACGGCAAAAGACGCTG‑GAgtgggt                                                                             >  4:111489‑M2/79‑276 (MQ=255)

                                                                                                                                                                        tgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCCGGTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTc‑gggtaaaagaacgtgaattg                                                                                 >  1:384905‑M2/79‑265 (MQ=255)

                                                                                                                                                                        tgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTGGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTg                                                                                >  1:204053‑M2/79‑275 (MQ=255)

                                                                                                                                                                        tgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCGGGTGGATGGAGCTGGAAGCGCAGGAGTCTCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAGGCAGGAGACTGGAAGAGTGTTC‑CGGTAAAAGACACGG‑AAGTGGtt                                                                             >  4:163327‑M2/79‑278 (MQ=255)

                                                                                                                                                                          cggttaccgagtggaaacccaaagaccgaattaatgaaataattctccgcactcggccagtatattcgtaaaggtgAG‑TGCAAAGATAATCGATTCTTTTTTGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGATGTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGa                                                                           <  4:379094‑M2/202‑1 (MQ=255)

                                                                                                                                                                             tgaccaagtggacacccgaagagcagattaacaaaaaaaattaccgccctaggcccgtatattcgtaaagggaAG‑TGCAAAGATAACCGATTCTTTTTCGCTTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGTGGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTg                                                                            <  3:159164‑M2/201‑1 (MQ=255)

                                                                                                                                                                               accgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCCGTTTGGGCTGTTCGATAAAGCAGGCCACTGGAAGAGTTTTC‑CGATAAAAG‑CAATGCAAGTTGTGGAACGa                                                                       >  4:540836‑M2/72‑277 (MQ=255)

                                                                                                                                                                               accgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATCCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTCCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACCG‑AAGTGGTTGaacca                                                                       >  2:569526‑M2/72‑275 (MQ=255)

                                                                                                                                                                                   agtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGaa                                                                                                                                                                      >  4:343573‑M2/68‑180 (MQ=255)

                                                                                                                                                                                    gtggtagcccgaagaacagattgaacaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGGTAATCGATTCTTTTTCGGTTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGCAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACt                                                                     <  3:531110‑M2/208‑1 (MQ=255)

                                                                                                                                                                                       gaaacccgaagagcaaattgataaaaaaatttaccgcactaggcccggatagtcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAGGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGca                                                               <  4:198929‑M2/214‑1 (MQ=255)

                                                                                                                                                                                         cacccgaagagcagattgaacaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGTTTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGca                                                               <  1:23033‑M2/214‑1 (MQ=255)

                                                                                                                                                                                         caaccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAAGCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGca                                                               <  1:160716‑M2/214‑1 (MQ=255)

                                                                                                                                                                                              gaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGCACAccc                                                          <  3:460830‑M2/219‑1 (MQ=255)

                                                                                                                                                                                                 gagcagattgatcaaaaaatttaccgcactaggcccgtacattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGTGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTCCCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CTGTAAAAGACAATG‑AAGTTGTTGAACGAATGGACCACACCCTGCgtt                                                    >  2:436821‑M2/54‑277 (MQ=255)

                                                                                                                                                                                                      gattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGCAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGCGCACACCCTTCGTTAg                                                  >  2:324882‑M2/49‑275 (MQ=255)

                                                                                                                                                                                                      gattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGa                                                                  >  2:392309‑M2/49‑259 (MQ=255)

                                                                                                                                                                                                      gattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGCACACCCTGCGTgat                                                  >  2:505331‑M2/49‑274 (MQ=255)

                                                                                                                                                                                                      gattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCGGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAACGACACTG‑AAGTGGTTGAACGAATGGAGCACACAATGCGTGAg                                                  >  4:239418‑M2/49‑275 (MQ=255)

                                                                                                                                                                                                       attgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAg                                                                 <  4:392309‑M2/212‑1 (MQ=255)

                                                                                                                                                                                                         tgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGtt                                                                                                                                                               >  3:28634‑M2/46‑165 (MQ=255)

                                                                                                                                                                                                            tcaaaaaatttaccgcactaggcccgtatattcgtgcaggtcAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCCACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATTGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACCGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGACGAGTGTTC‑CGGTAAAAGACACTG‑CAGTGGTTTAACGACTGTATCACACCCTGCGTGAGTTTCAc                                            >  1:418595‑M2/43‑275 (MQ=255)

                                                                                                                                                                                                            tcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACTTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCAc                                            <  1:673639‑M2/233‑1 (MQ=255)

                                                                                                                                                                                                            tcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCAc                                            >  1:747616‑M2/43‑275 (MQ=255)

                                                                                                                                                                                                             caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                                                                                                                                                           <  1:28634‑M2/124‑1 (MQ=255)

                                                                                                                                                                                                               aaaaagttaccggactaggcccgtatagtcgtgaatgtaAG‑GGCAAAGGTAATCCATTCTTTTTCTATTGGCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGaa                                       <  2:572608‑M2/238‑1 (MQ=255)

                                                                                                                                                                                                                aaaatttaccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTg                                                                                                                                          <  1:92055‑M2/141‑1 (MQ=255)

                                                                                                                                                                                                                      taccgcactaggcccgtatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAAGAGCtgg                                  >  3:64686‑M2/33‑274 (MQ=255)

                                                                                                                                                                                                                       accgcactagggccttatgttcgtgaaggtaAG‑TGCAAAGATAAACGGTGCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGGAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTg                               <  2:593000‑M2/246‑1 (MQ=255)

                                                                                                                                                                                                                          gcactaggcccgtatattagtgaaggtaAG‑TGCAAAGGTAATCGATTCTTTTTCGATTGGCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAAct                           <  2:539795‑M2/250‑1 (MQ=255)

                                                                                                                                                                                                                           ctataggcccgtatattcggtaaggtaAG‑TGCAAAGATAATCGATTCTTTGTCGATTGTCTGGCTGTAGGCGTCAACTGGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGaa                             <  3:578845‑M2/248‑1 (MQ=255)

                                                                                                                                                                                                                                ggcccgtatattcgtgaaggtaAG‑TGCAAATCTAAGCGATTCTTTTTCGAGTGGCTGGCTGTCTGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTgac                     <  4:25889‑M2/256‑1 (MQ=255)

                                                                                                                                                                                                                                    cgaatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCGTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGacgacg                 <  2:291803‑M2/260‑1 (MQ=255)

                                                                                                                                                                                                                                      tatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGTTGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTCTGGTCTGTTCGATAAAGTAGGCGACTGGACGATTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAAGGACTGGAGATCACTCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGacgacg                 >  2:432391‑M2/17‑276 (MQ=255)

                                                                                                                                                                                                                                      tatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGTAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGtgaactgctgcaga                   >  1:736529‑M2/17‑270 (MQ=255)

                                                                                                                                                                                                                                      tatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGCACACCCTGc                                                       >  2:352217‑M2/17‑238 (MQ=255)

                                                                                                                                                                                                                                      tatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTAACGAGCAGCTGCGTGAACTGctgacgca                  >  3:38370‑M2/17‑273 (MQ=255)

                                                                                                                                                                                                                                      tatattcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTAACGAGAAGCTGCGTGAACTGATGACGACgcg               >  4:84472‑M2/17‑277 (MQ=255)

                                                                                                                                                                                                                                          ttcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAAAGTGTTC‑CGGTAAAAGACACTG‑AGGTGGTTGACCGACTGGAGTACACCCTGCGGCAGTTTCAAGAGAAGTTGCGAGAACTGCTGACGCCGCTg              >  1:2097‑M2/13‑275 (MQ=255)

                                                                                                                                                                                                                                           tcgtgaaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTCCAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAGGAGTGTTC‑CGGTCAAAGACACTG‑AAGTGGTTGACAGACTGGAGCACACCCTGCGTGAGTAGCAAGAAACGATGCATGAACTTGTGACGACGCtgact           >  4:368559‑M2/12‑275 (MQ=255)

                                                                                                                                                                                                                                                aaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGTCTTGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGCCTGGGGCAAACCCTTCCTGAGTTTCACCAGAAGCTGCGTGAACTTTTTCCGACGCTGTATCTg        >  3:293513‑M2/7‑275 (MQ=255)

                                                                                                                                                                                                                                                aaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTg        >  1:97530‑M2/7‑275 (MQ=255)

                                                                                                                                                                                                                                                aaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTg        >  3:424422‑M2/7‑275 (MQ=255)

                                                                                                                                                                                                                                                aaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACCT‑AAGTGGTTGAACGGCTGGAGCACACCCTGCGTGATTTTCACGAGAAGCTGGGTGAGATGCGTACGACTCTGGACCGGAAg     >  4:439845‑M2/7‑278 (MQ=255)

                                                                                                                                                                                                                                                aaggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGCCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTGTTACCTACAGTTACCAGTTTGGTCTGCTCGATAAAGCAGGCGACTGGAAGAGCGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGCTTCACGCGAAGCTGCGTGAACTGCTGACGACGCTGAATCTg        >  1:155957‑M2/7‑275 (MQ=255)

                                                                                                                                                                                                                                                  ggtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACGG‑AAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAAATGCTGACGACGATGAAtctgatggt   >  2:467755‑M2/5‑274 (MQ=255)

                                                                                                                                                                                                                                                   gtaAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATATGAAg     >  1:306409‑M2/4‑275 (MQ=255)

                                                                                                                                                                                                                                                      aG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGCACACCCTGCGTGCGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCGGCATCTGAGGcgg  >  3:217664/1‑273 (MQ=255)

CATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAG‑TGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTC‑CGGTAAAAGACACTG‑AAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTG  >  NC_000913/258438‑258958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`