| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,535,934 | T→C | 100% | I34T (ATC→ACC) | crr → | glucose‑specific enzyme IIA component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,535,934 | 0 | T | C | 93.6% | 239.9 / 7.5 | 79 | I34T (ATC→ACC) | crr | glucose‑specific enzyme IIA component of PTS |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base C (36/36); total (38/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-02 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATTAAGAAGATTATCCGTAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATT > NC_000913/2535662‑2536201 | aTTGAGAAGATTATCCGTAACACGAACTTCGAAGGTGCGAAGGTGGTAGCAGAACAGGCTCTTGCTCAACCGACAACGGACGGGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAAGCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGGAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTAAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCg < 2:573339/275‑1 (MQ=255) gaagaGTTTCCGTAACACGAATGTGGAAGATGGGAAGGTGTTAGCAGAGCAGGATCTTGCTCAAACGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAATAAAAAACAATCTGCGAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGAc < 4:636658/275‑1 (MQ=255) acacGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGt > 2:393074/1‑275 (MQ=255) acacGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACANCTTAGGAGAAGATCAAGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTNTTGAGATCATTGCTCCGATCTCTGGCGAGANNGTCAATACCGAAGACGTGCCGGagatctgttnt > 3:95645/1‑269 (MQ=255) gAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTTAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTTCTTNGGAGAAGATCAGGGGTCTGTTCGATAAACTGAAATTTTTGGTTTCCGACGACATGAAGGATAACGGAACTATTGNGATCATTCCTCCCCTCTCTGGCTACAATGTNNATTACGAAAACGTCCCGACTGCGGtttttttng > 3:79717/1‑275 (MQ=255) aCTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGc > 2:349350/1‑275 (MQ=255) gATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGt < 1:127424/279‑1 (MQ=255) aaGGTGTTAGCAGAGCAGGATCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATACACTGAATTCCCTGGTTTCCGACGACAAGAAGTATACCGGCACTACTGATATCATTGTTCCGCCCTCTGGCGAGATTGTCACTACCGAAGCAGTGACGGATGTCGCTTTTGCGGAAAAACTCGTTg > 4:223597/1‑275 (MQ=255) ggTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGt < 2:375514/275‑1 (MQ=255) ggTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGt < 4:349349/275‑1 (MQ=255) ttAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTCACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCACTTTACTGCATAGGAGAAGATAATGGGTTTGTTCGATAAACTGAAAACTCTGGTTTCCGACGAACAGAAGGATATCGGAACTATTGGGATCATTTCTCCGCCGTCTGGCTAGACAGTCACTCCCCGAGCCGTACCCGGTGTCGTTTTTGCGGAAAAACTCCTTtgtggat > 4:225542/1‑272 (MQ=255) gagcagGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTAtt < 2:429263/274‑1 (MQ=255) agcagGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGaaaaa > 4:390331/1‑255 (MQ=255) ggCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCg < 2:390332/255‑1 (MQ=255) gttctTGCTCAACCGAAAACGGACGAGTTAATGAAGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGACGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATc < 4:254938/272‑1 (MQ=255) aaaaacGACAAAGAAAGAGTTAATGAGACTGGNTAACAAGTTAATTGAAGAAAAAACCNTCTGCTAATCCACGTAATGCGGCCCAATTTCCGGCTTAGGATAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTNTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGt < 3:113107/273‑1 (MQ=255) aaCCGAAAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACg < 4:393073/273‑1 (MQ=255) ccGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCGAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGACGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGt < 2:2775/274‑1 (MQ=255) aCGGGTTAATGAAGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCGGCGAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGt < 4:304048/275‑1 (MQ=255) aCGAGTTAATGACGCTGGTTAACAAGTTCATTGCAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGCTCATGGGTTTGTTCGATACACTGAAATCTCTGGTTTCCGACGACAAGACGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTCACAAAATGGt > 2:501277/1‑275 (MQ=255) gAGTTGATGACGCGGGGTAACAAGTCCATTGAAGAAAAAACAATCTGCTAATGCGCGGGGTGCGGGCCAATTTCCTGCTTAGGAGAGTATCATGGGTTTGTGTGATAAACGGAAGTTGCTTGTTTCCGACGACAAGATGGATACCGGAACTCTTGAGAGCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATAAAACCAACTGGTAACAAAATGGTcg < 4:294833/275‑1 (MQ=255) tGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACgg > 2:182109/1‑275 (MQ=255) gTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATg > 4:240987/1‑256 (MQ=255) aCAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTcg < 2:240988/256‑1 (MQ=255) gTTAATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACGGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGt < 1:205425/250‑1 (MQ=255) tCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTg > 4:14876/1‑208 (MQ=255) aTTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTaaa < 2:408606/274‑1 (MQ=255) tGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGa < 2:14876/208‑1 (MQ=255) cTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCCCCAGTAGACGGCCCCATTGGAAAAATCTTTAAAaccaacca > 4:375313/1‑274 (MQ=255) tGCTAATACACGAGATGCGGCCCAAGGTACTGCTTAGGAGAAGTTCATGGGTTTGTTCGATAAACTGTAATCTCTGGTTTCCGAAGACACGAAGGAGACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAATACGTGCCGGATGTCGTGTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCAtt < 1:206404/279‑1 (MQ=255) tAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAaaccaac < 2:375314/268‑1 (MQ=255) cGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGTCACCATTGGTAAAATCTTTGCAAACAACCCCCCATTCACTAt > 4:281956/1‑275 (MQ=255) cGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTAt < 4:303694/275‑1 (MQ=255) cGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAAc > 2:25340/1‑192 (MQ=255) ccAATTTACTGCTTAGGAGAAGATCAGTGGTTTGGTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAATCATCGACATCATTGCTCCGCTCTCTGGCGAGAGCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGAAGGTATTGCTATCAAACCAAc < 4:25340/192‑1 (MQ=255) tttACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAAcc > 2:234587/1‑185 (MQ=255) cTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACg < 4:234586/185‑1 (MQ=255) tGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGCATCTGATAGCGGCGTTGaa > 1:16158/1‑277 (MQ=255) tGCTTAGGAGAAGATCAGAGTTTTGTTCGATGAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATGGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTa < 1:73597/254‑1 (MQ=255) tAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACt > 4:297840/1‑275 (MQ=255) aGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGCCGGCACCATTGGTAAAATCTTTGAAACCAACCACGCTTTCCCTATCGAATCTGATAGCCGCGTTCaaacg > 4:333499/1‑272 (MQ=255) aGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGACGTCGTTTTTGCGGAAAACATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACAACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTg > 2:401712/1‑275 (MQ=255) aTCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCa < 4:182108/275‑1 (MQ=255) aTCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAAACAACCAAGCATTCTCTATCGAATATTATAGCGGCGTTTAACTGTTCGTCCACtt > 1:14966/1‑278 (MQ=255) tGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAAc > 2:673204/1‑166 (MQ=255) tGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAaac > 2:26713/1‑220 (MQ=255) tGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTc > 2:93187/1‑275 (MQ=255) ggggTTGTTCGTAAAACTGAAATCGATGGATGCCGACGACAAGAAGGATGACGGAACTATTGAGATCATGGCTCCGCTCTCTGACGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGTGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTc < 4:158539/274‑1 (MQ=255) tttgtagaaaaaaCTGAAATTTGTGGNNTCCGCNGACAAGAAAGATACCTGAACTTTTGAGAGCAGTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGa < 3:16158/269‑1 (MQ=255) tttGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGt < 4:673203/166‑1 (MQ=255) tttGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAaaccaac < 4:26713/220‑1 (MQ=255) tttGTTCGATAAACTGAAATCGCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTACGCTCTCTGGCGAGATCGTCAATACCGAAGTCGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGa < 1:79717/279‑1 (MQ=255) tGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGt > 2:188793/1‑272 (MQ=255) ttCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGa < 2:440768/275‑1 (MQ=255) tAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGAGCGTAAATACCGAAGACGTGCCGGCTGTCGTTTTTGCGGAAAAAATAGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACCAAATGGTCGCGCCATTATACGGCCACATTCGCAACAGCTTTGAAACCAACCACTCATTCTCTACCGAATCTGATAGCGCCGTTGAACTGTTCCGCCACTCCGGTCTCGACAGCg > 4:233175/1‑275 (MQ=255) ctGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGaa < 1:95645/279‑1 (MQ=255) tGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGACGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCTCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACCCATTCCCTACCAAATCTGATAGGGGCGTTGACATGTTCGTCCACTTCGGTATCGACCCCGTTTAACGGAAAgga > 4:635665/1‑274 (MQ=255) ggTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCg < 2:354587/275‑1 (MQ=255) agGATACCGGAACGATAGAGATCATTGTACCGCTCTCTGGCGAGATCGTCAATAACGAAGACGGGCCGGATGTCGTTGTTGCGGAAAAAATCGTTGGGGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTAtt < 2:7356/275‑1 (MQ=255) aTACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCCACATTGGTACAATCTTTGAAACCAACCAAGCAATCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCCATTCGGTATCCAAACCGTTTAACTGAAAAGCGAAGGATTCAAGCGTATTGCt > 4:163860/1‑275 (MQ=255) aCCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGaaaaa > 2:311963/1‑84 (MQ=255) gAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCg < 4:311962/84‑1 (MQ=255) aTTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGt < 4:188792/273‑1 (MQ=255) aTTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATCGGGAAAAGCTTTGAAACCAACCAAGCATTCGCTATCGAATCTGATAACGGCGTTGAACTGTTCGTTCACTTCGGTATCAACACCGTTAAACTTAAAGGCGAAGGTTTCCAGCGTTGTGATGAAGGAGGTAACCGCGTgca > 4:265003/1‑273 (MQ=255) tGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGtt > 3:108283/1‑70 (MQ=255) tGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGtt < 1:108283/70‑1 (MQ=255) cGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATg > 2:49779/1‑72 (MQ=255) cGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGc > 2:265271/1‑275 (MQ=255) ctctctGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATg > 2:709578/1‑102 (MQ=255) ctctctGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTg > 2:693745/1‑169 (MQ=255) ctctctGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAgcggc > 4:566489/1‑177 (MQ=255) ctctGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTAt < 4:49779/72‑1 (MQ=255) ctGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTcg < 4:709577/102‑1 (MQ=255) ctGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAg < 4:693744/169‑1 (MQ=255) ctGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTg < 2:566490/177‑1 (MQ=255) agactgTCACTACCGAAGACGTGACGGATGTCGTTTTTGAGGAAAAAATCTTTGGTGATGGTATTTCTATAAAACCAACGGGTAACACAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCAt < 2:482459/269‑1 (MQ=255) agaTCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGACCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCAAGCATTACATATCGAATCTGATAGCGTAGTTGAACTGTTCGTCCACTTCGTGATCGAANCCGTTGAACTTAAAGGCAAAGGCTTCAAGCGTATTTCGGAAGAAGGTCAGCGCGTAAAAGTTGGAGATACtagccgtgaat > 3:168941/1‑269 (MQ=255) tCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCAtt > 2:291722/1‑272 (MQ=255) gTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAAtt > 4:406239/1‑275 (MQ=255) | ATTAAGAAGATTATCCGTAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATT > NC_000913/2535662‑2536201 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |