| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,750,922 | G→A | 5.1% | C31Y (TGC→TAC) | nadK → | NAD kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,750,922 | 0 | G | A | 5.1% | 250.6 / 2.4 | 78 | C31Y (TGC→TAC) | nadK | NAD kinase |
| Reads supporting (aligned to +/- strand): ref base G (41/33); new base A (2/2); total (43/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
GATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGATATTAAAGTTATTGGAATCAACCGTGGCAACCTGGGTTTCCTGACTGACCTTGACCCCGATAACGCCCAGCAACAGTTAGCCGATGTGCTGGAAGGCCACTAC > NC_000913/2750648‑2751193 | gATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCtgtg > 2:602633/1‑275 (MQ=255) tcctttaTAATTTCTTCCTGGGTTTTCCCCTCTNTCTTTTTCTTTTTTTTACTACTCATNAATTTCTCCTGTGTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTAGGGGGATCAGTTTCAGGGTTTCAAGGGAAGCANTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACaaa < 3:135498/273‑1 (MQ=255) ttcttcCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTAc < 2:365888/275‑1 (MQ=255) gggCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCAt < 2:570923/275‑1 (MQ=255) cAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGAGGGACACCCACGGCACCCCACTGAACTGACAACACATGAAATGCTATACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGCGcaa > 2:180514/1‑273 (MQ=255) aGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGcaa > 2:626323/1‑272 (MQ=255) tGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACg < 4:602632/275‑1 (MQ=255) tactacTCCTGAATTTCCCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGCTCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCCCCCCACTGCACTGACAACCCATGAAATGCTCTCCCCCTGCCTGTGCACCAAAGGTTACGAGGTCCTCGTTGACCAACAAATCGCTCACGAACTgat > 4:449766/1‑273 (MQ=255) tactacTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCa > 2:126018/1‑275 (MQ=255) tactacTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAAAACATGAAATGCTCTACCGCTGGCTGTGca > 4:525565/1‑226 (MQ=255) actCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACaaa < 2:525566/226‑1 (MQ=255) actCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTGTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCg < 2:449767/258‑1 (MQ=255) ctCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATGGTCAACAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTCCAAGTGTATTAGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCAGCGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAACCGCACACAAACGGAAAc > 4:560195/1‑272 (MQ=255) ctcCGCGTTTTTTTCGCATTCATCTCGCTACCTTCGCATATTAGAGGGGTCAGTTTCAGGGTTTCAAGGTAAGCACTCACATTGTGATCAATCTTCGAAACAAGGACCTCGGAAAAAGGAATAATCATTTAAAGTGTATTGGCATTTTGGGACACGCACGGCACCACACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATAGTTGAGCAACAAATCGCTCACGACCGACAACTGAAGAAttttcaa > 4:207672/1‑269 (MQ=255) cgtgTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGAATTGTGGGAGACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCa < 1:87615/276‑1 (MQ=255) tttttCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACg < 4:126018/275‑1 (MQ=255) atGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTcgcg > 2:408685/1‑275 (MQ=255) tCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCCCGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTtgg > 1:24515/1‑279 (MQ=255) cAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTACACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGAAACTGAAGAATGTGAAAACTGGCACGCTCGCGGCGCTGGGGCAACGAGCTGAACCCGCGGTAGCCGt > 4:281990/1‑275 (MQ=255) aGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGCAGAATGTGAAAACTGGAACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGtt > 2:652851/1‑275 (MQ=255) gggTTTCAAGGGAAGCACTAACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGc < 4:626322/274‑1 (MQ=255) gTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTAGTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTCTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGa < 2:207673/274‑1 (MQ=255) tCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGt > 2:563407/1‑275 (MQ=255) aGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGc > 2:165395/1‑274 (MQ=255) cacaTTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGgcgc < 2:433807/275‑1 (MQ=255) acaTTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCCCGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAACCTGGCACGCCCGCGGACATTGGGCAAGTAGCGAATCCCGCGGTAGTCGGTTGGGCCGGGGGTAAAATGCAGGgcgg > 4:493980/1‑273 (MQ=255) aTTGTCATCAATATTCGCAACAAGGAACTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTTTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGGCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGgcacg < 1:120826/278‑1 (MQ=255) atcaATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTTCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAAGCTCTCACGAACTGCAACTGAAGAATGTCAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGCTAGCCGTTGGGTGCGACGGTAATA‑GCCGGGGGCGggaccggac > 4:419536/1‑270 (MQ=255) atcaATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAACGGTTACGAGGTCATCGTTGAGCAACAAATCGCGCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGTTAAACTGATGGGCGCGgcccggca > 4:619568/1‑272 (MQ=255) atcaATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTACACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTtggtg > 2:617875/1‑244 (MQ=255) tcaATCTTCGCAACAAGTAACCAGGAAAAATGAATAATCATTTCAAGTGTATTGGCATGGTGGGACACCCACGGCACCCAAATGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGcaca < 2:321135/275‑1 (MQ=255) caATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGcaca < 2:25062/274‑1 (MQ=255) aTCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTACACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGa < 4:617874/244‑1 (MQ=255) tCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCCCGCGGAGATTCGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCCCACTCGCCCGTTa > 1:96484/1‑279 (MQ=255) cGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTAc > 1:212727/1‑279 (MQ=255) gCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGGGGCGACGGTAATATGCTGGGCGCGGCACGCAAACTCGCCCGt > 2:285168/1‑275 (MQ=255) acaaGGACCTCGGAAAAATGAAGAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCAACCCACTGCACTGACAACACATTAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTa < 2:245160/274‑1 (MQ=255) ggACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTgg < 3:128026/177‑1 (MQ=255) ggACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTgg > 1:128026/1‑177 (MQ=255) tATTGGCATTGTGGGACACCCACTGCACCCCACTCCCATGACAACACATGAAATGCTCTACAGCTGGCGGTGAACAAAAGGTTACGAGGTAATCGTTGAGCAACAAATCGCTGACGAACTGCAACTGAAGAATGTGAAAACTGGAACGCGCGCGGAGAGTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGATATTAAAGTTATTGGAATCAACCGTGGCAACCTGGGttt < 1:132402/279‑1 (MQ=255) tATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGCACTGCAACTGAAGAATGTGAANACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCCAGCGGTAGCCGTGGGTGGCGATGGTAATATGCTTGGGGGGGCNCGGCCACCCGCGCGTTATCCTCTTANAGTGATTGGAATAGACCGTGGCCACCTGGGtt > 3:137842/1‑278 (MQ=255) tATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGATATTAAAGTTATTGGAATCAACCGTGGCAACCTg > 2:602328/1‑274 (MQ=255) ttGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAANTGGCACGCTAGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGNGGCCCGCCCACTCGCCCGTCACGAAATTAANNATATTGGAATCAACGTGGGCAACCTggggt > 3:90519/1‑274 (MQ=255) ttGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTtggtg > 4:658207/1‑192 (MQ=255) cATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGa < 2:658208/192‑1 (MQ=255) ttGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGATATTAAAGTTATTGGAATCACCCGTGGCACCCTGGGTTTCct > 2:378998/1‑274 (MQ=255) ttGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGATATTAAAGTTATTGGAATCAACCGTGGCAACCTGGGTTTCct > 2:559808/1‑274 (MQ=255) tttgGGAGACCCACGGCACCCCACCGCATTGATAGCACAGGAACAGCCCTACCGCCGGATTTGCACAAAAGGTTAGGAGGTCGTCGTGGAGCAACAAGACGCGCCCGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCCGAGAGTGGGCAACTAGCTGATCTCGCGGTAGGCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGATATTAAAGTTATTGGAATCAACCGTGGCAACCTGGGTTTCctga < 4:165394/273‑1 (MQ=255) tgtgGGACCCCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGATATTAAAGTTATTGGAATCAACCGTGGCAACCTGGGTTTCctgact < 1:90519/277‑1 (MQ=255) gtgGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTgg > 2:726830/1‑134 (MQ=255) gtgGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGa > 1:35159/1‑188 (MQ=255) gtgGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGa < 3:35159/188‑1 (MQ=255) gtgGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGATATTAAAGTTATTGGAATCAACCGTGGCAACCTGGGTTTCctgac > 2:644014/1‑275 (MQ=255) gACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACg < 4:726829/134‑1 (MQ=255) acacCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTAGCGGTAGTCGTTGGTGGCGAGGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGATATTAAAGTTATAGGTATCAACCGTGGAAACCTGGGTCTCCTGACTGACCTTg > 1:177011/1‑278 (MQ=255) ccGGCACCCCACGGAACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCAGAAAAGGTTACGCGGGCATCGATGGGCAACAAATCGCGCACGAACTTCAACTGAAGAATGTGAAAACTGGCAGGGTCGCGGGGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTGGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGATATTAAAGTTATTGGAATCAACCGTGGCAACCTGGGTTTCCTGACTGACCTTGAc < 4:360941/273‑1 (MQ=255) aCGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTcg < 1:109208/158‑1 (MQ=255) aCGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTcg > 3:109208/1‑158 (MQ=255) aCGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTtggtg > 2:457597/1‑173 (MQ=255) aCGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGAGGCACGCACACTCGCCCGTTACGATATTAAAGTTAtt > 4:47593/1‑232 (MQ=255) cACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGa < 4:457596/173‑1 (MQ=255) cACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGATATTAAAGTTATTGGaa < 2:47593/232‑1 (MQ=255) ccccACTGCACTGACAACAAATGAAATGCCCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGATATTAAAGTTATTGGAATCAACCGTGGCAACCTGGGTTTCCTGACTGACCTTGACCCCGATAACGc < 1:62147/279‑1 (MQ=255) ccACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTtgg > 4:710284/1‑163 (MQ=255) ctgcactgACAACACATGAAATGCTATACCGCTGGCTGTACACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGATATTAAAGTTATTGGAATCAACCGTGGCAACCTGGGTTTCCTGACTGACCTTGACCCCGATAACGcc < 2:281991/275‑1 (MQ=255) cgcgaatgACAACAAATGAAAATCCCTACCGCCGGCGTGGCGCAAAAGGTTCCGAGATCATCGTTGAGAAGCAAAGCGCTCACGAGATTCAACTGAAGAAGGTGAAAAATGGCACGGTCGCGGAGATTGGGCAACTAGCTGGTCTGGCGTTGGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGATATTAAAGTTATTGGAATCAACCGTGGCAACCTGGGTTTCCTGACTGACCTTGACCCCGATAACGcc < 4:563406/269‑1 (MQ=255) tgcactgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGc < 2:710285/163‑1 (MQ=255) ctgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGATATTAAAGTTATTGGAATCAACCGTGGCAACCTGGGTTTCCTGACTGACCTTGACCCCGATAACGCCCAGCa > 4:273038/1‑275 (MQ=255) acaacaCATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGgcacg > 4:277191/1‑182 (MQ=255) acaacaCATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAAATGGCACGCTCGCGGAGATTGGGCAAc > 4:675985/1‑127 (MQ=255) cacaTGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGc < 2:675986/127‑1 (MQ=255) cacaTGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGcaca < 2:277192/182‑1 (MQ=255) acaTGAAATGCGCAACCGCTGGCTGTGCACAAAAGGGTACGAGATCAGCGTGGAGCAACAAATCGCGCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGAGCTCGCGGTAGTCGGTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGATATTAAAGTTATTGGAATCAACCGTGGCAACCTGGGTTTCCTGACTGACCTTGACCCCGATAACGCCCAGCAACAGTTAGCCg < 1:202498/278‑1 (MQ=255) ctctACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGATATTAAAGTTATTGGAATCAACCGTGGCAACCTGGGTTTCCTGACTGACCTTGACCCCGATAACGCCCAGCAAAAGTTAACCGTTGTTCt > 4:651408/1‑275 (MQ=255) ctACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGATATTAAAGTTATTGGAATCAACCGTGGCAACCTGGGTTTCCTGACTGACCTTGACCCCGATAACGCCCAGCAACAGTTAGCCGATGTGCTg > 2:37235/1‑274 (MQ=255) aCCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCGACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGATATTAAAGTTATTGGAATCAACCGTGGCAACCTGGGTTTCCTGACTGACCTTGACCCCGATAACGCCCAGCAACAGTTAGCCGATGTGCTGGaa < 2:185763/275‑1 (MQ=255) ccGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGATATTAAAGTTATTGGAATCAACCGTGGCAACCTGGGTTTCCTGACTGACCTTGACCCCGATAACGCCCAGCAACAGTTAGCCGATGTGCTGGAAg > 2:481557/1‑275 (MQ=255) ggctgTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTtggtg > 2:713173/1‑129 (MQ=255) gctgTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGCTATTAAAGTTATTGGAATCAACCGTGGCAACCTGGGTTTCCTGACTGACCTTTACCCCGATAACGcc > 2:657685/1‑241 (MQ=255) tgtgCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCGCGCACACTCGCCCGTTAAGATATTGAAGTTATTGGAATCAACCGTGGCAACCTGGGTTTCCTGACTGAACTTGACCCCGATAACGCCCAGCAACAGTTAGCCGATGTGCAGGAAGGCCACTAc > 2:204653/1‑275 (MQ=255) gtgCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGa < 4:713172/129‑1 (MQ=255) gCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATNTCGCGGTAGTCGTTGGTGGCGACNNTAATATGCNGGGCGCGTCACGCACACTCGCCCGTTa > 3:43029/1‑166 (MQ=255) gCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTa < 1:43029/166‑1 (MQ=255) | GATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACGGTAATATGCTGGGCGCGGCACGCACACTCGCCCGTTACGATATTAAAGTTATTGGAATCAACCGTGGCAACCTGGGTTTCCTGACTGACCTTGACCCCGATAACGCCCAGCAACAGTTAGCCGATGTGCTGGAAGGCCACTAC > NC_000913/2750648‑2751193 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |