Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,115,770 | C→T | 6.3% | A485T (GCA→ACA) | glpK ← | glycerol kinase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,115,770 | 0 | C | T | 6.3% | 187.7 / 4.2 | 66 | A485T (GCA→ACA) | glpK | glycerol kinase |
Reads supporting (aligned to +/- strand): ref base C (38/23); new base T (2/2); total (40/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.35e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.19e-01 |
CGCCGCGTCCTAACCATTTGTAGCCAGCCAGCGCCGCTGATTCGGTGACGCGGGAAAATTCGATGGCAAGTTCTCGTCTCATAGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGC > NC_000913/4115498‑4116047 | cgcctcGTCCTAACCCGTTGTAGACGGCCAGCGCCGCTGATTCGGTGACGCGGGAAAATTCGATGGCAAGTTCTCGTCTCATAGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGt < 4:121692/275‑1 (MQ=255) cgccgcGTCCTAACCATTTGTAGCCAGCCAGCGCCGCTGATTCGGTGACGCGGGAAAATTCGATGGCAAGTTCTCGTCTCATAGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGt < 2:336096/275‑1 (MQ=255) cgcgTCCTAACCATTTGTAGCCAGCCAGCGCCGCTGATTCGGTGACGCGGGAAAATTCGATGGCAAGTTCTCGTCTCATAGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTGCCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAAc > 2:431664/1‑275 (MQ=255) tgggcctaccattttGTGGCCGGCCAGGGACGGTGATTCGGGGAAGGGGGGAAATTCGAGGGCAAGGGCACGTTTCATAGCAAACTGTAAGCAGTAAGGAAGGGCGCGAAGTGTAGCAAATGGGAAGGGAGGGATTATGGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCAGTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAAc < 4:456348/262‑1 (MQ=255) tttGTAGCCAGCCAGCGCCGCTGATTCGGTGACGCGGGAAAATTCGATGGCAAGTTCTCGTCTCATAGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGCGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCCTGTTCTTCCCACGCCATCCCTCGTTTAACCGCTTTTTTCCACCCTTCTTACCGGTAAtttccct > 4:74935/1‑269 (MQ=255) tGTAGCCAGCCAGCGCCGCTGATTCGGTGACGCGGGAAAATTCGATGGCAAGTTCTCGTCTCATAGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAg > 2:373251/1‑275 (MQ=255) gagccgcGGTTTCGGGGACGCGGGAAAATTCGATGGCAAGTTCTCGTATAATAGGAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGc < 4:373250/273‑1 (MQ=255) gcTGATTCGGTGACGCGGGAAAATTCGATGGCAAGTTCTCGGCTCATAGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGCGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGa < 2:74935/275‑1 (MQ=255) gcgGGAAAATTCGATGGCAAGTTCTCGTCTCATAGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGCCGGAACTCGCGCt > 2:356863/1‑274 (MQ=255) gcgGGAAAATTCGATGGCAAGTTCTCGTCTCATAGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAAt > 1:90949/1‑278 (MQ=255) aaTTGGATGGCAAGTTCGCGGTTCATAGCAAACTCTAAGCAGTAAGGAAGGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCAc < 2:380566/274‑1 (MQ=255) ggtgtgggttaaTAGCAAATTCTAGGCAGTAAGGAAGGGGGGGAAGGGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCa < 4:74974/264‑1 (MQ=255) tgCAAACTTGAAGGAGTAAGGAAGGGCGCGAAGTGAAGCACAGGGGAAGGTAGGGATTAGTGATGTGTGCGGGGTTGCCCCCGCACTTTTCGGGACGACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGGGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCCGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGtt < 4:399162/274‑1 (MQ=255) aCTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCAc > 2:297219/1‑243 (MQ=255) tAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATCCGGCATTTGCATGATTCGTCGTGTTCTTCCCACGCCATCGCGCGTATAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCCCAGTGGTTTCGATGCCTGTACGGAACCCGCGCCCAATCCCCGCTTTCGCTTACAGCACGGCGGCGTTACGGCAGAAGCCaaa > 4:248358/1‑273 (MQ=255) aaGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCCGTGGTTTCGATGCCTGGACGGAACTCGCCCCCAATCCCCGCTTTCCATTTCAGCCCGTCGAGGTTCTGCCAGCAGCCCAccc > 4:660112/1‑274 (MQ=255) aaTGGCGCGAAGTGGAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAg < 2:357530/275‑1 (MQ=255) tGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTTGTTTCGATGCCTGGTCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCCAGGTTCCGCCAGAAGCCAACCGCCAgc > 2:217000/1‑273 (MQ=255) ggCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGc > 2:27795/1‑274 (MQ=255) cgcgAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCCGGCCt > 4:242484/1‑274 (MQ=255) cgcgAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCt > 2:503080/1‑274 (MQ=255) cgcgAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCCCTCAATAACCGCTTTCTGTTTCAGATCCTCGCGGTTCTGCCCGCAACCAACCACCAGCCCt > 4:573878/1‑274 (MQ=255) tAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCCAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGCTAgg > 2:277536/1‑275 (MQ=255) gggAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTAc > 2:148645/1‑162 (MQ=255) gggAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCt < 1:183466/209‑1 (MQ=255) gggAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCt > 3:183466/1‑209 (MQ=255) agggagggATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTc < 4:148644/162‑1 (MQ=255) gtgtgCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCGCCGCTTTCGCTTTCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCCCCATGCCTGCGAGATAGGCCGCACCCAATAGGGTGACCTCGGCCACt > 4:493719/1‑275 (MQ=255) gtgtgCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCCGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCCCGCAc > 2:191770/1‑274 (MQ=255) tgtgCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGCACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGCTAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCg > 1:130771/1‑278 (MQ=255) gtgCGGGGTTGCCCCCGCACGTTTCCGGACCNNGGGATGCTGAATAAACGCTTCATTGGGCATNTACATTATTCGGCGTGTTCTTCCCACGCAATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTANGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCg < 3:90949/277‑1 (MQ=255) tgCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCACCAGTCCTGCGAGATAGGCCGCACCCACTGCGGTGACTTCGCGCCCTTcc > 2:90811/1‑275 (MQ=255) gCGNGTNTGCCCCCTCACGTTTCTGGACTACCGGATGCGGCATAAGCGCTTCAGNCGGCATGTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGa < 4:727934/215‑1 (MQ=255) ggTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGCAGCCACCCGCCAGGCCTCCGAGCTGGGCCGACCCCAATCCGGTGACTTCGCCAACTCCCGGgc > 4:256268/1‑274 (MQ=255) cccccGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGCGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCaa > 2:121319/1‑275 (MQ=255) cccccGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCaa > 2:569726/1‑275 (MQ=255) cccccGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTCTCCATCCTGCGTAACGGTAATCACGCTCAGTGGTTTCGATGCCTGGACGGAACACGCGCGCAATAACCGCTTTCGCTTGAAGCTCGTCCAGGTTCTGCCAGAAGCCAACCGCCATGCCTGCAAGCAAGGCCGCACCCACTGCGGTCACTACGCGCCCTCCAGGGCGCCCaa > 4:67817/1‑275 (MQ=255) gCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGCCTTCGCGCCCTTCCGGGCCCTCAACGCgg > 2:372150/1‑275 (MQ=255) tACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGc > 2:273488/1‑218 (MQ=255) ccGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGCTAGGCCGCACCCAATCCGGTGACTTCGCGCACTTCCGGGCCCTCCACCCCGGTCCCGAACATATcc > 2:117350/1‑273 (MQ=255) ccGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACCCCGGTGCCGACAatgacgg > 4:572466/1‑270 (MQ=255) ccGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCCAGATCGGCCGCACCAAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACCCGGGTGCCGAGaaatgcg > 4:551210/1‑269 (MQ=255) ggATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCAccc < 4:273487/218‑1 (MQ=255) cATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGt > 2:200550/1‑218 (MQ=255) aaCGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACt < 4:200549/218‑1 (MQ=255) tCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGAt > 4:388931/1‑189 (MQ=255) gcagtgggCATTTTCCTTAGTCGTCTTGGTTTTACCACGCCAACGCGCGTTTAATCTCTTTTTTTCAGCCTGCGTAACGGTAATGGCGCTCAGTGGTGTCGATGCCTTGCCTGAACTCGCGCTCGATCACCGCTTTCTCTTGCAGCTCGTCGCGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTTCCGAGAATATCGGACTGGAACTGCATCAGGaaa < 4:290408/269‑1 (MQ=255) tCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGc < 2:388932/189‑1 (MQ=255) aTTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTGTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCt < 2:493720/275‑1 (MQ=255) tACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACt < 2:548188/275‑1 (MQ=255) aCATTTTTCGTGGTGTGCTTCCCACGCCATCGCGCGTTTAACCGCTGTTGTCCAGCCTGCGTAACGGGAATTACGCTCTGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAAGAACCGCTTGTTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTg < 4:356862/275‑1 (MQ=255) attCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTTACTTCCCCGCCTTCCGGGCGCTCAACGCGGGTGCCGACAATATCGGACTGGAACTGCATCCGGAAATTTTTTGCTACTGccccc > 4:548187/1‑274 (MQ=255) attCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCg > 2:722116/1‑83 (MQ=255) attCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGGTAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGGAAATCGGAATGGAACTGCATCAGGAAATTTTTTGCTTCTGCCcca > 4:478287/1‑275 (MQ=255) attCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCCCGCCCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCCCTTCCGGGCGCCCCACCCGGGTGCCGCGCATCCCGGCATGGAACTGCCGCCGGACATTGTTTCCCACtacccc > 4:74891/1‑269 (MQ=255) gtcgtGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGc < 4:722115/83‑1 (MQ=255) gtcgtGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTa < 2:478288/263‑1 (MQ=255) cgtGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCCCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCCCACCCAATTCGGGCACCTCCGGCACTTCCGGGCGCCCAACGCGGGTGCCGAGAATATAGGACGGGAACTGCATCAGGAACCTGTTTGCTACTCAGCCCTCATcc > 4:4870/1‑275 (MQ=255) gtattggcccgggcATCGCGCGTTTAACCGCCTGTTGCCAGCCTGCGTAACGTGAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAAGCACCGCTTTCTCTTGGAGCTCGTGGAGGTTGTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCAcg < 4:191769/262‑1 (MQ=255) ttttcCCAGGCCATGTGGCGTTGAACCGCATTTNGCAGGCCAGCGAAACGGGAATTACGNTCAGTGGTTTCGGTGCCGGGACGGAACTCGCGCTCAATCACCGCTTTCTCTGGCCGCGCGGCGAGGTTCGGCCAGAAGCCNACCGCCAGGCCGGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAgg < 3:130771/277‑1 (MQ=255) tcttcCCACGCCATCGGGCGTTTAACCGCTTTTTTCCGGCCTGGGTAACGGGAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCAcgcg < 4:569725/275‑1 (MQ=255) cccACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCCCCATCCACGCCCAgg > 2:533184/1‑275 (MQ=255) cccACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAgg > 2:310122/1‑275 (MQ=255) cgcgcgTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCCGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAACATATCGGACTGGAACTGCCTCAGGAAATTGTTTGCTCATGCGCCCCCATCCACGCGCAGGGCGTGCCGCCGGac > 1:139419/1‑278 (MQ=255) tttAACCCCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATAc < 2:4870/275‑1 (MQ=255) ccTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAgg > 2:487826/1‑124 (MQ=255) cGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGc < 4:487825/124‑1 (MQ=255) cGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACGGCGCCACCATCCACGCGCCGGGCGTGCAGACGGATACCAGAGTCGGACTGCATCGCTTCCatc > 1:79041/1‑276 (MQ=255) | CGCCGCGTCCTAACCATTTGTAGCCAGCCAGCGCCGCTGATTCGGTGACGCGGGAAAATTCGATGGCAAGTTCTCGTCTCATAGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGC > NC_000913/4115498‑4116047 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |