Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,116,538 T→G 93.8% N229H (AAC→CAC)  glpK ← glycerol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,116,5380TG93.8% 259.9 / 6.3 80N229H (AAC→CAC) glpKglycerol kinase
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base G (35/40);  total (37/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

TCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGC  >  NC_000913/4116267‑4116807
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                             
tCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGACTACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTg                                                                                                                                                                                                                                                                         >  1:173899/1‑278 (MQ=255)
 cgcgCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGAGATCCCGGCGCTTGGAATCCGCGTGCCGCCTTTGCCGCCAATGTGAGTc                                                                                                                                                                                                                                                                           >  2:493216/1‑275 (MQ=255)
     cAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGta                                                                                                                                                                                                                                                                   <  1:56985/279‑1 (MQ=255)
                    tGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGCCGATCCCGGAGATTGGAATACGCGTGCCGTCTTTGCCGCCAATGTGATTCTCACCGTATTCTTCGtaaa                                                                                                                                                                                                                                                        >  4:239166/1‑274 (MQ=255)
                          tGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAacga                                                                                                                                                                                                                                                   >  2:87053/1‑274 (MQ=255)
                            ccagacaccccGCTCCTCCCAAGGCTTAGTTCCCTCCGCCAGTCGGTCCCCACGGGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTCACCGCCCGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGc                                                                                                                                                                                                                                                <  4:659371/266‑1 (MQ=255)
                            ccATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCTCGGAGATTGGAATACGCGTGTCGCCTTTGCCGCCAATGTGCGTCTGACCGTATACTTCGGAAGAacgacg                                                                                                                                                                                                                                                 >  2:65823/1‑274 (MQ=255)
                                aagcacCGAACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTGTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACt                                                                                                                                                                                                                                             <  2:6595/271‑1 (MQ=255)
                                      cGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGta                                                                                                                                                                                                                                                                   <  1:22565/246‑1 (MQ=255)
                                      cGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCCTATACTTCGGAAGAACGACGCCCTTCTGgca                                                                                                                                                                                                                                      >  2:177158/1‑275 (MQ=255)
                                                              ttCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAac                                                                                                                                                                                                                                                     >  2:442132/1‑236 (MQ=255)
                                                              ttCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCCCTTc                                                                                                                                                                                                          >  1:5997/1‑279 (MQ=255)
                                                                                   ggggtgatgaggcagGCCCTTTTCTGAATTCACCGCTTTCTCGCCAGGGTTCATCGGCATAAAGCGTCCAGTGCCCTAGGTGTTCTTCGCCCTCCCTTCTTTCACGCACAACTGACCAAACCGCGCGGCATGCTGGTTACCGGCGATCCCGGAGATTGGAATACTCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTcc                                                                                                                                                                                          <  4:634829/263‑1 (MQ=255)
                                                                                    aTGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTGCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTc                                                                                                                                                                                     <  1:62344/278‑1 (MQ=255)
                                                                                        tggtCAGCAGGCCGTTTTCTGATTTCACCGCTTTCGCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTc                                                                                                                                                                                     <  2:610395/274‑1 (MQ=255)
                                                                                        tggtCAGCAGGCCGTGTTCTGATTTTACGCGTTTTTCGCCAGTGGTCATCAGCATAAAGCAGCCAGTGACATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTc                                                                                                                                                                                     <  4:87053/274‑1 (MQ=255)
                                                                                         ggtCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCa                                                                                                                                                                                   <  2:248419/275‑1 (MQ=255)
                                                                                          gtCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCATGGTTCATAAACATAACGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCGTTCACGCACAACTGACCAAACAGCGCGGCCTGCTTGTCACCGGCGATCCCGGGGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCa                                                                                                                                                                                   <  4:557203/274‑1 (MQ=255)
                                                                                               cagGCCGTTTTCTGATTTCACCGCTTTCGCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATATGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTAt                                                                                                                                                                             <  1:195579/275‑1 (MQ=255)
                                                                                                atgCCGGTTTGTGATTTCACCGTCTGTTCGCCANAGTTAATCCGGAGACAGCAAAAATGNCCGTAGGTGTTCGTCGGCCTCCCTACCATCACGCACAGCTGGCCACACCGAGCGGCCTGCTGGGCCCCGGCGATCCCGGANGTTGGGGTGCGCGTGCCGCCTTGGCCGCCAATTTGAGTCTGTGCGTATACTTCGGAAGGACGACGCGCTTCTGGCAGCATCTCGCGGGGAGTATGCAGCACTTTCAGCAATTTGTCGTCCCAGTCCAGGGTATGGATg                                                                                                                                                                        <  3:115745/277‑1 (MQ=255)
                                                                                                agGCCGTTTTCTGATTTCACCGCTTTCCCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCGGGGtct                                                                                                                                                                             >  2:634830/1‑272 (MQ=255)
                                                                                                        ttCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGa                                                                                                                                                                    >  2:78898/1‑275 (MQ=255)
                                                                                                                tCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTgtc                                                                                                                                                                                              >  2:419919/1‑241 (MQ=255)
                                                                                                                       tttCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGACGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACgag                                                                                                                                                     <  2:239167/275‑1 (MQ=255)
                                                                                                                       gtttgggccaaggtTCATCGGCAGAAAGAAGACAGTGCCATGGGTGTGCTTCGCCAGCGCTTGTTTCACGCCCAACTGACCAAACAGCGCGGCGTGCTGGTCACCGGCTATACCGGGGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGGCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACgag                                                                                                                                                     <  4:65823/263‑1 (MQ=255)
                                                                                                                        ttCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACgaga                                                                                                                                                    <  2:432675/275‑1 (MQ=255)
                                                                                                                           tcGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACGTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCGAGCACTTCCAGCATTTTGTCGTcc                                                                                                                                                                                          <  2:134707/234‑1 (MQ=255)
                                                                                                                                 gtgtTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGgt                                                                                                                                           >  2:644455/1‑275 (MQ=255)
                                                                                                                                 gtgtTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGgt                                                                                                                                           <  2:554598/275‑1 (MQ=255)
                                                                                                                                    ttCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCCTTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGCGAGGCGTTGGTGTa                                                                                                                                        >  2:560285/1‑275 (MQ=255)
                                                                                                                                       atcaGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATc                                                                                                                                     >  2:586241/1‑275 (MQ=255)
                                                                                                                                               aaaGCAGCCAGTGCCATAGGTGCTCCTCCCCATCCCATCGTTCACGACCAACTGGCCCAACAGCGCGGCCTGCTGGTCACGNGGGATCCCGGGGATTGGAATACCGGTGCCGCCTTTGCCGGCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGac                                                                                                                         <  3:173899/279‑1 (MQ=255)
                                                                                                                                                aaGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCGTGCACGCACAAATGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGATATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGaca                                                                                                                        <  1:158323/279‑1 (MQ=255)
                                                                                                                                                 agcagcCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGgaagaa                                                                                                                                                                                                                                                      >  2:265642/1‑152 (MQ=255)
                                                                                                                                                     gcCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAacgac                                                                                                                                                                                                                                                  <  4:265641/152‑1 (MQ=255)
                                                                                                                                                      ccAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCTCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATTTTGAATAACATGGTACGAGAGGCGTTGGTGTAATCGGTCCCATGGACACg                                                                                                                      >  2:309538/1‑275 (MQ=255)
                                                                                                                                                      ccAGTGCCATAGGTGTTCTTCGACATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGTCGATCCCGGCGATTGGAATACGCGTGCAGCCTTTGCCGCCAATGtt                                                                                                                                                                                                                                                                               >  4:709743/1‑122 (MQ=255)
                                                                                                                                                          tgtcATAGGTTTTTTTCGCCCTCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTc                                                                                                                                                                                                                                                                           <  2:709744/119‑1 (MQ=255)
                                                                                                                                                          tGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGNAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATAACCAGCACTTCCAGCAGTTTTTCTTCCCCGTCACGGGTACGGANGGTGAACAACATGGAACTAAAAGCGNTGGAGTAATTGGACACAGGGGCACAGcctcgtg                                                                                                              >  3:128400/1‑274 (MQ=255)
                                                                                                                                                            ccATAGGGGTTCTCCGCCACCCCTTCTGTCACGAACAACTGACCAAACAGCGCGGCCGGCTGGTCCCCGGCGATCCCGGGGATTGGAATACGCGGTCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTg                                                                                                                <  4:380270/275‑1 (MQ=255)
                                                                                                                                                             cATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAacgacg                                                                                                                                                                                                                                                 <  3:124351/145‑1 (MQ=255)
                                                                                                                                                             cATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAacgacg                                                                                                                                                                                                                                                 >  1:124351/1‑145 (MQ=255)
                                                                                                                                                                    gtTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGCCGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATTTTGAACAACATGGTCCGAGAGGCGTTGGGGTAATCGGTAACATGTAAACGGCCCTGAGtactt                                                                                                         >  4:519769/1‑270 (MQ=255)
                                                                                                                                                                    gtTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTgtc                                                                                                                                                                                              >  2:486510/1‑189 (MQ=255)
                                                                                                                                                                    gtTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATg                                                                                                                                                                        <  1:72877/211‑1 (MQ=255)
                                                                                                                                                                    gtTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCAttt                                                                                                        >  2:46136/1‑275 (MQ=255)
                                                                                                                                                                    gtTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTAACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTTCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTATGAGAGGCGTTGGTTTAACCGGTCACATGACCACTGCCATGAGTCAttt                                                                                                        >  2:632917/1‑275 (MQ=255)
                                                                                                                                                                      tcttcGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAg                                                                                                                                                                                       >  4:104434/1‑194 (MQ=255)
                                                                                                                                                                        ttcGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTcc                                                                                                                                                                                          <  4:486509/189‑1 (MQ=255)
                                                                                                                                                                          cGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCa                                                                                                                                                                                   <  2:104434/194‑1 (MQ=255)
                                                                                                                                                                                           cGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCTGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGCATGTTGAACACCATGGTACGAAGGGCGTTGGTTTAATCGGTCCAAGTGACCCGCCCCTGAGCAATTTTCCAAATAATCCACGttacaccc                                                                                 >  4:73668/1‑268 (MQ=255)
                                                                                                                                                                                           cGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCa                                                                                                                                                                                        >  2:680831/1‑172 (MQ=255)
                                                                                                                                                                                               caACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTcc                                                                                                                                                                                    <  4:680830/172‑1 (MQ=255)
                                                                                                                                                                                                   tGACCAAAAGGCGCGGCCTGCTGGGCACCGGCGATCCCGGGGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCaaa                                                                         <  2:90993/275‑1 (MQ=255)
                                                                                                                                                                                                     aCCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCAtt                                                                                                                                                                                                   >  2:628777/1‑151 (MQ=255)
                                                                                                                                                                                                         aaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTgt                                                                                                                                                                                               <  4:628776/151‑1 (MQ=255)
                                                                                                                                                                                                          aCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGagag                                                                                                                                                   >  4:281410/1‑194 (MQ=255)
                                                                                                                                                                                                              cgcgGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGt                                                                                                                                               <  2:281411/194‑1 (MQ=255)
                                                                                                                                                                                                               gcgGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACgg                                                                                                                     >  4:301611/1‑219 (MQ=255)
                                                                                                                                                                                                                   ccTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCt                                                                                                                 <  2:301612/219‑1 (MQ=255)
                                                                                                                                                                                                                      gctgGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAAGCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGt                                                      <  2:303223/275‑1 (MQ=255)
                                                                                                                                                                                                                           tccccGGGGATCCCGGGAATTGGAAACAGCTTGCCGCCTTTGCTGCCAATTGGAGTCGTACCGTGTACTTCGGAAGAACGACGAAATTCTGGCGGCATGTCGCTCGGAACATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATACGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACg                                                 <  4:560614/272‑1 (MQ=255)
                                                                                                                                                                                                                                 gCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAAACGCAATTCATCACAACGTGCCCGCCCGCGagag                                       >  1:149658/1‑279 (MQ=255)
                                                                                                                                                                                                                                    aTCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGTCAGCATCTAGCGCGGAATATCCAGCACTTCCAGAATTTTGTCGTCCCAGTCCAgg                                                                                                                                                                                 >  4:689759/1‑138 (MQ=255)
                                                                                                                                                                                                                                        cGGAGATTGGAATACGCGTGCCGCCTTTGCCGCAAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTGCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGGCGTCCCAGTCCAGGGTAt                                                                                                                                                                             <  2:689760/138‑1 (MQ=255)
                                                                                                                                                                                                                                          gagaTTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACGTGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCcaca                              <  1:128400/279‑1 (MQ=255)
                                                                                                                                                                                                                                            gaTTGGAATCGCCGTGCCGCCTTTCCCGCCAATGTGAGTCTGACCGGTTACTTCGGAGGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCGGGTAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCca                                <  4:46136/275‑1 (MQ=255)
                                                                                                                                                                                                                                               tGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCAtt                                                                                                                                                                                                   >  4:703453/1‑109 (MQ=255)
                                                                                                                                                                                                                                               tGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTccc                                                                                                                                                                                         >  2:193202/1‑119 (MQ=255)
                                                                                                                                                                                                                                                ggAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGt                                                                                                                                                                       >  4:380395/1‑136 (MQ=255)
                                                                                                                                                                                                                                                  atgacgctgcccgtcTTTGCCTCACAGGTGAGTCGTACAGTTTACTTCGGACGATCGACGCCCTTCCGGGAGCAGTTCGAGCGGAATATCCAGCACTTCCAGCATTTTGTCGGTCCAGTCCAGTGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGt                          <  4:560284/265‑1 (MQ=255)
                                                                                                                                                                                                                                                   aTACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTgt                                                                                                                                                                                               <  2:703454/109‑1 (MQ=255)
                                                                                                                                                                                                                                                   aTACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCGGTc                                                                                                                                                                                     <  4:193201/119‑1 (MQ=255)
                                                                                                                                                                                                                                                    tACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGaa                                                                                                                                                                   <  2:380396/136‑1 (MQ=255)
                                                                                                                                                                                                                                                       gcgTGCGGCCTTTGCCGCCAAGGTGAGTCTGACCGTATACTTCGGACGACGGACGTACTTCTGGCAGCAGCTCGTGCGGAATATCCAGGCCTTCCAGCATTTTGTCGTCCCAGTCCAGGGGATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAgg                     <  4:404789/275‑1 (MQ=255)
                                                                                                                                                                                                                                                            ccgccTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATcc                 >  2:447890/1‑274 (MQ=255)
                                                                                                                                                                                                                                                            ccgccTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGa                                                                                                                          >  4:87676/1‑169 (MQ=255)
                                                                                                                                                                                                                                                            ccgccTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATTCACCGTACCAAACAGCAATTCCCCCCGCCGTGCACGCTCGCGAGCTCCTTCCACATGGTCCAgcaccc                 >  4:63996/1‑269 (MQ=255)
                                                                                                                                                                                                                                                            ccgccTTTGCCGCCAATGTGAGTCTGACCGAATACTTCGGAAGACCGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGTCGCTGGTGTAATCGGTCACATGGCCACGGCCCCGTGTCATTTTCCAGATAAGCCACGCACCAACCGTACCAACCTCCAATTCACCACGACTTGCAGGCTCGCCACAACCTTCCCTATGGTCGAGCATcc                 >  4:463023/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                cTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACg                                                                                                                      <  2:87676/169‑1 (MQ=255)
                                                                                                                                                                                                                                                                          aaTGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGc  <  4:447889/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                             
TCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGC  >  NC_000913/4116267‑4116807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: