| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,116,538 | T→G | 93.8% | N229H (AAC→CAC) | glpK ← | glycerol kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,116,538 | 0 | T | G | 93.8% | 259.9 / 6.3 | 80 | N229H (AAC→CAC) | glpK | glycerol kinase |
| Reads supporting (aligned to +/- strand): ref base T (2/3); new base G (35/40); total (37/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01 | |||||||||||
TCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGC > NC_000913/4116267‑4116807 | tCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGACTACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTg > 1:173899/1‑278 (MQ=255) cgcgCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGAGATCCCGGCGCTTGGAATCCGCGTGCCGCCTTTGCCGCCAATGTGAGTc > 2:493216/1‑275 (MQ=255) cAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGta < 1:56985/279‑1 (MQ=255) tGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGCCGATCCCGGAGATTGGAATACGCGTGCCGTCTTTGCCGCCAATGTGATTCTCACCGTATTCTTCGtaaa > 4:239166/1‑274 (MQ=255) tGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAacga > 2:87053/1‑274 (MQ=255) ccagacaccccGCTCCTCCCAAGGCTTAGTTCCCTCCGCCAGTCGGTCCCCACGGGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTCACCGCCCGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGc < 4:659371/266‑1 (MQ=255) ccATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCTCGGAGATTGGAATACGCGTGTCGCCTTTGCCGCCAATGTGCGTCTGACCGTATACTTCGGAAGAacgacg > 2:65823/1‑274 (MQ=255) aagcacCGAACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTGTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACt < 2:6595/271‑1 (MQ=255) cGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGta < 1:22565/246‑1 (MQ=255) cGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCCTATACTTCGGAAGAACGACGCCCTTCTGgca > 2:177158/1‑275 (MQ=255) ttCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAac > 2:442132/1‑236 (MQ=255) ttCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCCCTTc > 1:5997/1‑279 (MQ=255) ggggtgatgaggcagGCCCTTTTCTGAATTCACCGCTTTCTCGCCAGGGTTCATCGGCATAAAGCGTCCAGTGCCCTAGGTGTTCTTCGCCCTCCCTTCTTTCACGCACAACTGACCAAACCGCGCGGCATGCTGGTTACCGGCGATCCCGGAGATTGGAATACTCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTcc < 4:634829/263‑1 (MQ=255) aTGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTGCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTc < 1:62344/278‑1 (MQ=255) tggtCAGCAGGCCGTTTTCTGATTTCACCGCTTTCGCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTc < 2:610395/274‑1 (MQ=255) tggtCAGCAGGCCGTGTTCTGATTTTACGCGTTTTTCGCCAGTGGTCATCAGCATAAAGCAGCCAGTGACATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTc < 4:87053/274‑1 (MQ=255) ggtCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCa < 2:248419/275‑1 (MQ=255) gtCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCATGGTTCATAAACATAACGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCGTTCACGCACAACTGACCAAACAGCGCGGCCTGCTTGTCACCGGCGATCCCGGGGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCa < 4:557203/274‑1 (MQ=255) cagGCCGTTTTCTGATTTCACCGCTTTCGCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATATGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTAt < 1:195579/275‑1 (MQ=255) atgCCGGTTTGTGATTTCACCGTCTGTTCGCCANAGTTAATCCGGAGACAGCAAAAATGNCCGTAGGTGTTCGTCGGCCTCCCTACCATCACGCACAGCTGGCCACACCGAGCGGCCTGCTGGGCCCCGGCGATCCCGGANGTTGGGGTGCGCGTGCCGCCTTGGCCGCCAATTTGAGTCTGTGCGTATACTTCGGAAGGACGACGCGCTTCTGGCAGCATCTCGCGGGGAGTATGCAGCACTTTCAGCAATTTGTCGTCCCAGTCCAGGGTATGGATg < 3:115745/277‑1 (MQ=255) agGCCGTTTTCTGATTTCACCGCTTTCCCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCGGGGtct > 2:634830/1‑272 (MQ=255) ttCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGa > 2:78898/1‑275 (MQ=255) tCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTgtc > 2:419919/1‑241 (MQ=255) tttCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGACGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACgag < 2:239167/275‑1 (MQ=255) gtttgggccaaggtTCATCGGCAGAAAGAAGACAGTGCCATGGGTGTGCTTCGCCAGCGCTTGTTTCACGCCCAACTGACCAAACAGCGCGGCGTGCTGGTCACCGGCTATACCGGGGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGGCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACgag < 4:65823/263‑1 (MQ=255) ttCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACgaga < 2:432675/275‑1 (MQ=255) tcGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACGTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCGAGCACTTCCAGCATTTTGTCGTcc < 2:134707/234‑1 (MQ=255) gtgtTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGgt > 2:644455/1‑275 (MQ=255) gtgtTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGgt < 2:554598/275‑1 (MQ=255) ttCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCCTTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGCGAGGCGTTGGTGTa > 2:560285/1‑275 (MQ=255) atcaGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATc > 2:586241/1‑275 (MQ=255) aaaGCAGCCAGTGCCATAGGTGCTCCTCCCCATCCCATCGTTCACGACCAACTGGCCCAACAGCGCGGCCTGCTGGTCACGNGGGATCCCGGGGATTGGAATACCGGTGCCGCCTTTGCCGGCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGac < 3:173899/279‑1 (MQ=255) aaGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCGTGCACGCACAAATGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGATATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGaca < 1:158323/279‑1 (MQ=255) agcagcCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGgaagaa > 2:265642/1‑152 (MQ=255) gcCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAacgac < 4:265641/152‑1 (MQ=255) ccAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCTCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATTTTGAATAACATGGTACGAGAGGCGTTGGTGTAATCGGTCCCATGGACACg > 2:309538/1‑275 (MQ=255) ccAGTGCCATAGGTGTTCTTCGACATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGTCGATCCCGGCGATTGGAATACGCGTGCAGCCTTTGCCGCCAATGtt > 4:709743/1‑122 (MQ=255) tgtcATAGGTTTTTTTCGCCCTCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTc < 2:709744/119‑1 (MQ=255) tGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGNAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATAACCAGCACTTCCAGCAGTTTTTCTTCCCCGTCACGGGTACGGANGGTGAACAACATGGAACTAAAAGCGNTGGAGTAATTGGACACAGGGGCACAGcctcgtg > 3:128400/1‑274 (MQ=255) ccATAGGGGTTCTCCGCCACCCCTTCTGTCACGAACAACTGACCAAACAGCGCGGCCGGCTGGTCCCCGGCGATCCCGGGGATTGGAATACGCGGTCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTg < 4:380270/275‑1 (MQ=255) cATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAacgacg < 3:124351/145‑1 (MQ=255) cATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAacgacg > 1:124351/1‑145 (MQ=255) gtTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGCCGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATTTTGAACAACATGGTCCGAGAGGCGTTGGGGTAATCGGTAACATGTAAACGGCCCTGAGtactt > 4:519769/1‑270 (MQ=255) gtTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTgtc > 2:486510/1‑189 (MQ=255) gtTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATg < 1:72877/211‑1 (MQ=255) gtTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCAttt > 2:46136/1‑275 (MQ=255) gtTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTAACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTTCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTATGAGAGGCGTTGGTTTAACCGGTCACATGACCACTGCCATGAGTCAttt > 2:632917/1‑275 (MQ=255) tcttcGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAg > 4:104434/1‑194 (MQ=255) ttcGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTcc < 4:486509/189‑1 (MQ=255) cGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCa < 2:104434/194‑1 (MQ=255) cGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCTGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGCATGTTGAACACCATGGTACGAAGGGCGTTGGTTTAATCGGTCCAAGTGACCCGCCCCTGAGCAATTTTCCAAATAATCCACGttacaccc > 4:73668/1‑268 (MQ=255) cGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCa > 2:680831/1‑172 (MQ=255) caACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTcc < 4:680830/172‑1 (MQ=255) tGACCAAAAGGCGCGGCCTGCTGGGCACCGGCGATCCCGGGGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCaaa < 2:90993/275‑1 (MQ=255) aCCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCAtt > 2:628777/1‑151 (MQ=255) aaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTgt < 4:628776/151‑1 (MQ=255) aCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGagag > 4:281410/1‑194 (MQ=255) cgcgGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGt < 2:281411/194‑1 (MQ=255) gcgGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACgg > 4:301611/1‑219 (MQ=255) ccTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCt < 2:301612/219‑1 (MQ=255) gctgGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAAGCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGt < 2:303223/275‑1 (MQ=255) tccccGGGGATCCCGGGAATTGGAAACAGCTTGCCGCCTTTGCTGCCAATTGGAGTCGTACCGTGTACTTCGGAAGAACGACGAAATTCTGGCGGCATGTCGCTCGGAACATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATACGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACg < 4:560614/272‑1 (MQ=255) gCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAAACGCAATTCATCACAACGTGCCCGCCCGCGagag > 1:149658/1‑279 (MQ=255) aTCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGTCAGCATCTAGCGCGGAATATCCAGCACTTCCAGAATTTTGTCGTCCCAGTCCAgg > 4:689759/1‑138 (MQ=255) cGGAGATTGGAATACGCGTGCCGCCTTTGCCGCAAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTGCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGGCGTCCCAGTCCAGGGTAt < 2:689760/138‑1 (MQ=255) gagaTTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACGTGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCcaca < 1:128400/279‑1 (MQ=255) gaTTGGAATCGCCGTGCCGCCTTTCCCGCCAATGTGAGTCTGACCGGTTACTTCGGAGGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCGGGTAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCca < 4:46136/275‑1 (MQ=255) tGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCAtt > 4:703453/1‑109 (MQ=255) tGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTccc > 2:193202/1‑119 (MQ=255) ggAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGt > 4:380395/1‑136 (MQ=255) atgacgctgcccgtcTTTGCCTCACAGGTGAGTCGTACAGTTTACTTCGGACGATCGACGCCCTTCCGGGAGCAGTTCGAGCGGAATATCCAGCACTTCCAGCATTTTGTCGGTCCAGTCCAGTGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGt < 4:560284/265‑1 (MQ=255) aTACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTgt < 2:703454/109‑1 (MQ=255) aTACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCGGTc < 4:193201/119‑1 (MQ=255) tACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGaa < 2:380396/136‑1 (MQ=255) gcgTGCGGCCTTTGCCGCCAAGGTGAGTCTGACCGTATACTTCGGACGACGGACGTACTTCTGGCAGCAGCTCGTGCGGAATATCCAGGCCTTCCAGCATTTTGTCGTCCCAGTCCAGGGGATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAgg < 4:404789/275‑1 (MQ=255) ccgccTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATcc > 2:447890/1‑274 (MQ=255) ccgccTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGa > 4:87676/1‑169 (MQ=255) ccgccTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATTCACCGTACCAAACAGCAATTCCCCCCGCCGTGCACGCTCGCGAGCTCCTTCCACATGGTCCAgcaccc > 4:63996/1‑269 (MQ=255) ccgccTTTGCCGCCAATGTGAGTCTGACCGAATACTTCGGAAGACCGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGTCGCTGGTGTAATCGGTCACATGGCCACGGCCCCGTGTCATTTTCCAGATAAGCCACGCACCAACCGTACCAACCTCCAATTCACCACGACTTGCAGGCTCGCCACAACCTTCCCTATGGTCGAGCATcc > 4:463023/1‑274 (MQ=255) cTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACg < 2:87676/169‑1 (MQ=255) aaTGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGc < 4:447889/275‑1 (MQ=255) | TCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGC > NC_000913/4116267‑4116807 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |