Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,685,527 A→G 100% G354G (GGT→GGC fumC ← fumarate hydratase (fumarase C),aerobic Class II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,685,5270AG100.0% 196.3 / ‑6.7 60G354G (GGT→GGCfumCfumarate hydratase (fumarase C),aerobic Class II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (27/29);  minor base T (2/1);  total (30/30)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TACCCCAGCGCAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGC  >  NC_000913/1685258‑1685784
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                 
tACCCCAGCGCAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAgc                                                                                                                                                                                                                                                           <  1:81202/278‑1 (MQ=255)
      cgcgcAATGGCCGCAGCTTTTAAGGTCATCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGcccccc                                                                                                                                                                                                                                                    <  1:82187/278‑1 (MQ=255)
       gcgcAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCATGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGcc                                                                                                                                                                                                                                                        >  2:418058/1‑274 (MQ=255)
           aatggCCGCAGCTGTGAAGGTCAGCCCTTCTGTTTGCGCTTTTTTGGCGATCTCGGCGGCTTGTTCATAACCAATGTGGGTGGTAAGGGCAGTCACCAGCAACAGCGATTCATTGAGTAAGTGATTGATTCGCTCACGATTCGGTGCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCa                                                                                                                                                                                                                                                   <  4:229294/272‑1 (MQ=255)
           aaGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCa                                                                                                                                                                                                                                                   <  2:475203/275‑1 (MQ=255)
               ggcgaggCTTTTAAGGTGGGCCGTTTTTTGTGGGCTTTTGTGGCGGTCTCGGCGGCGTTGTCATAACCAATGTGGGTGTTAATCGGAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGGGGATCAGCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGtt                                                                                                                                                                                                                                               <  4:57724/269‑1 (MQ=255)
                        tttAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGTGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCTCCAGCATCAGCGATTCATTTAGTAATTTCTTGATTCGCTCACGCTTCGGTTCCATACCCACTGCGAAGTGTTTGTTAAAACTTTCCATGCCATTTGCCAGCACGGCCTCCGCTTGCAGTACATTGTGGATCCCCATCTGACGGAAGACTTTCAATTCAATGTTTCCGGAACCCCCACCCATGTTTCTCGCCa                                                                                                                                                                                                                                       >  4:509665/1‑274 (MQ=255)
                         ttAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCCGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCCCCGATTGCAGGAAATTGTGGATCACCATTGGAAGGACTGCGTTCAGTTCAAAGTACCCGGAAGCGCCCCCCATGTTGACCCCCag                                                                                                                                                                                                                                      >  4:355181/1‑273 (MQ=255)
                             agtacggccTTGTTTTTGGGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGACTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCAcgtcg                                                                                                                                                                                                                                  <  4:317952/267‑1 (MQ=255)
                                      ttcttTTTGCGCTGTTTTGGCGATCTCGGAGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCa                                                                                                                                                                                                                        <  4:207999/275‑1 (MQ=255)
                                      ttcttTATGCGCTTTTTTTGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAATCGCAGTCACCAGCATCAGCGATTCATTGAGTAAGTGATTGCGTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCa                                                                                                                                                                                                                        <  4:18263/275‑1 (MQ=255)
                                                  ttttttGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTACGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATTCCATCTGCCAGCAATCGCCCCGATTGCAGGAAATTGTTGATCCCCATTGGCCGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCCTGTTTATCGCAACCTCCGTCCCCATCCTCTGAcagcag                                                                                                                                                                                                             >  4:230082/1‑274 (MQ=255)
                                                  ttttttGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagc                                                                                                                                                                                                               <  2:355182/272‑1 (MQ=255)
                                                           gATCTCTGCGGCTTTTTCTAAACCCATGTGGGTGTTAAGCGCTGTCCCCAGCATCAGCGATTCATTTAGTAATTGATTGATTCGCTCACTATTCGGTTCAAGACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAACGCACCGATTGCAGGAAATTGTGGGTCGCCATTAGACGGAAGACGTTCAGTTCAAAGTGGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCAACACCTGACAGCAGAGCATGGTTa                                                                                                                                                                                                   <  4:124027/275‑1 (MQ=255)
                                                           aagtccggcggCTTTGTCATAACCAATGTTGGGGTTAACCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGAATCGCACACCATTGGGTTCAACACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGCCGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCAGGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                                                                                                                                   <  4:418057/270‑1 (MQ=255)
                                                            aTCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTTGATAACCATTGGACGGAAGACGTCCAGTTCAAAGTTTCCGGAAGCGCCCCCCATGTTGATCGCCCCTTCCTTCCCCAGCACCTGCCAGCAGACCATGGt                                                                                                                                                                                                     >  4:126250/1‑272 (MQ=255)
                                                                    tgcTTTGTCGTAACCAAGGTTGGTGTTAAGCGGCATTACCATCATCCGGGATTCATTGCGTATTTGATTGATTTGTTCACGATTCGGTTCACTATCCCCTGCGCGGTGGTTGTTAAATCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGTAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGGGCCCCCCATGTTAGTCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcac                                                                                                                                                                                          <  4:282754/274‑1 (MQ=255)
                                                                                     tgtgGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACt                                                                                                                                                                         >  2:279470/1‑275 (MQ=255)
                                                                                     tgtgGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACt                                                                                                                                                                         >  2:22598/1‑275 (MQ=255)
                                                                                     tgtgGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAAAGCAGAGCATGGTTAATGCCTCAAACTGTGTTGGGTTCACt                                                                                                                                                                         >  2:505774/1‑275 (MQ=255)
                                                                                     tgtgGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGACCCCCATGTTGATCGCCACGTCGTTCCCCATCCCCTGACGGCAGAGCATGGTTAATGCCTCACACTGTGCTGGCTTCAc                                                                                                                                                                          >  4:307822/1‑274 (MQ=255)
                                                                                         ggTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTgtgt                                                                                                                                                                                   <  2:307823/261‑1 (MQ=255)
                                                                                          gtgtTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTcc                                                                                                                                                                     <  2:211873/274‑1 (MQ=255)
                                                                                             ttAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCCTCACCTGACAGACGAGCATGGTTAATCCCTCACACTGTGTTGGGTTCACTTTCCCCg                                                                                                                                                                  >  2:31064/1‑274 (MQ=255)
                                                                                             ttAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCCCCCTGTGTTGGGTTCACTTTCCCCg                                                                                                                                                                  >  2:372538/1‑274 (MQ=255)
                                                                                             ttAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCgg                                                                                                                                                                 <  2:188901/275‑1 (MQ=255)
                                                                                                     aGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCACAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTg                                                                                                                                                         >  2:131901/1‑275 (MQ=255)
                                                                                                                 cAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCt                                                                                                                                             >  2:450097/1‑275 (MQ=255)
                                                                                                                                     tgttgattGGCTCACGGTTCGATTCAATACCCACTGTGCAGTGTTTGTTAAAACGTTCCATGCCAACAGCCGACAAGCGCAACGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACTTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGAt                                                                                                                          <  4:131901/272‑1 (MQ=255)
                                                                                                                                          attCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagca                                                                                                                                                                                                              >  4:180538/1‑185 (MQ=255)
                                                                                                                                          attCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGAATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCAcc                                                                                                                    >  2:355621/1‑275 (MQ=255)
                                                                                                                                              gCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                                                                                                                                          <  2:180539/185‑1 (MQ=255)
                                                                                                                                                      tgcGGATCAATAACCACTGCGCAGGGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCAGCGATTGCAGGGAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAgcg                                                                                                        <  2:509666/273‑1 (MQ=255)
                                                                                                                                                                     aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGgccag                                                                                         >  2:618479/1‑275 (MQ=255)
                                                                                                                                                                     aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGACCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCGCACACTGTGTTGGGTTCACTTTCCCCGGCAGGCTTGAGCTGCCCGGCTCCTTTTCCGGGAGTGAGATTTCACCAATTCCGCAGCGCGGGCCagcgccccg                                                                                         >  4:357176/1‑268 (MQ=255)
                                                                                                                                                                       tGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCAc                                                                                                                                                                                                                                      >  2:635370/1‑132 (MQ=255)
                                                                                                                                                                         cgtaGTGTTTGTTAAAACTTTCCATGCCAGCTGCCAGCAAGCGCACCGATTGCATGAAATTGTGTATCACCTTTGGACGGAAGACTTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGcc                                                                                                                                                                                              <  2:638071/167‑1 (MQ=255)
                                                                                                                                                                           cAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCAcgtcg                                                                                                                                                                                                                                  <  4:635369/132‑1 (MQ=255)
                                                                                                                                                                                             tCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCGGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGCCATCATTGGCGAttt                                                                  >  2:125808/1‑274 (MQ=255)
                                                                                                                                                                                             tCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGa                                                                                                                                                                                                                                             >  4:380562/1‑103 (MQ=255)
                                                                                                                                                                                                 tGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGc                                                                                                                                                                                                                                         <  2:380563/103‑1 (MQ=255)
                                                                                                                                                                                                      tcggCCAGCAAGAGCACCGATGGCAGGAAATTGTGGGTCACCATTGGACGGGAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTGCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACGTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATGGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGa                                                        <  4:52643/272‑1 (MQ=255)
                                                                                                                                                                                                                               ggAAATTGGGGATCCCCCTGGGACGGACGGCGTGCAGTTCAAAGTTTCCGGAAGCGCCCCCCAGGTTGATCGCCACGTCGTTCCCCATCCCCTGACAGCAGAGCATGGTTAATGCCTCAGACTGTGTTGGGTTCACTTTCCCCTGCATGATTGAGCGGCCCGGCTCATGTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCg                               <  4:154340/275‑1 (MQ=255)
                                                                                                                                                                                                                                 aaaTTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGt                              <  2:88643/274‑1 (MQ=255)
                                                                                                                                                                                                                                    ttttgGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGCCAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGAGTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTgcgc                          <  4:607576/272‑1 (MQ=255)
                                                                                                                                                                                                                                     tgtgGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGCCGCAGCCAACCCTTTCAAcgaccagttcgcc                         >  4:534082/1‑264 (MQ=255)
                                                                                                                                                                                                                                      gtgtATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCCGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAAc                    <  1:22936/279‑1 (MQ=255)
                                                                                                                                                                                                                                        ggagaaacagtGGGCGGATGGCGTTCAGTTCAAAGGTGCCGGAGGGGCCCCCCATGTTGATCGCCACGGCTTTCCCCATCACCTGACAGCAGAGCATGGTTAATGGTTCACACTGTTTGGGGTTCACTTGACCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCTCCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATTAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGcc                         <  4:254868/262‑1 (MQ=255)
                                                                                                                                                                                                                                                 ttGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCCGCGCGGGCCAGAGGCCAGCCAGCGGACATCATCGGCGCTTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAAccctggc              >  4:293545/1‑269 (MQ=255)
                                                                                                                                                                                                                                                 ttGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGc              >  2:506680/1‑274 (MQ=255)
                                                                                                                                                                                                                                                    gACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagca                                                                                                                                                                                                              >  4:700519/1‑79 (MQ=255)
                                                                                                                                                                                                                                                    gACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGTGCCAGAGGCCAGCCAGCGGACATCATTGTCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGACCACGGCCCTCACAg       >  1:136706/1‑278 (MQ=255)
                                                                                                                                                                                                                                                        gAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                                                                                                                                          <  2:700520/79‑1 (MQ=255)
                                                                                                                                                                                                                                                         aaGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGcc                                                                                                                                                                                              >  1:113463/1‑90 (MQ=255)
                                                                                                                                                                                                                                                         aaGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGcc                                                                                                                                                                                              <  3:113463/90‑1 (MQ=255)
                                                                                                                                                                                                                                                         aaGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGAtt                                                                                                                               >  3:111659/1‑153 (MQ=255)
                                                                                                                                                                                                                                                         aaGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGAtt                                                                                                                               <  1:111659/153‑1 (MQ=255)
                                                                                                                                                                                                                                                         aaGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTCCCGGGCTTGAGATTTCAATAATTCCGCAGCGCGTGCCAGATGCCAGCCACCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCCACCCTCTACACGCCCCTTTCGCCTGACCCAgtgtctcccagtg     >  4:170942/1‑263 (MQ=255)
                                                                                                                                                                                                                                                         aaGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCAGGATTGAGCTGCCCGGCTCATTTTGCGGGCTTGAGATTTCACCAATTCCGCAGCGTGTGCCAGAGGCCCGCCAGCGGCCATCATTGGCGATTTTCATCAGTGACCCCGCCAACCCTTTCCACNNNCCGTGGGCCTGCACAACGGCaaccccagtcca  >  3:194104/1‑267 (MQ=255)
                                                                                                                                                                                                                                                         aaGACGTTCAGTTCAAAGCTGCCGGAAGCGCCCCCCCGGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGCTTCACTTTCCACGGCATGATTGAGCTGCACGGCCCATTTTCCGTGATTGAGATTTCACCAATTCCGCAGCACGGCCCAGCGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCTACCACCCCATTAACCGCGCCGTGCTCCTGAAACAGGgcagcaacggt     >  4:638431/1‑270 (MQ=255)
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                 
TACCCCAGCGCAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGC  >  NC_000913/1685258‑1685784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: